Restriction map for
pF1KB8184
(Circular) (Six-base) MAPSORT of: pF1KB8184.seq Check: 6286 from: 1 to: 4079
pF1KB8184 4079 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 688 775 688
Size: 87 3992
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1904 2336 3083 3351 643
Size: 1261 432 747 268 1371
Fragments arranged by size:
1371 1261 747 432 268
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AleI CACnn'nnGTG
Cuts at: 518 518
Size: 4079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 617 617
Size: 4079
AlwNI CAG_nnn'CTG
Cuts at: 439 1384 2951 439
Size: 945 1567 1567
Fragments arranged by size:
1567 1567 945
ApaLI G'TGCA_C
Cuts at: 2849 2849
Size: 4079
ApoI r'AATT_y
Cuts at: 982 1083 3432 3688 982
Size: 101 2349 256 1373
Fragments arranged by size:
2349 1373 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 274 1099 274
Size: 825 3254
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 700 1095 1509 1757 1792 700
Size: 395 414 248 35 2987
Fragments arranged by size:
2987 414 395 248 35
BanII G_rGCy'C
Cuts at: 311 1093 2123 311
Size: 782 1030 2267
Fragments arranged by size:
2267 1030 782
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 683 649
Size: 34 4045
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BglI GCCn_nnn'nGGC
Cuts at: 253 427 915 253
Size: 174 488 3417
Fragments arranged by size:
3417 488 174
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 541 1191 541
Size: 650 3429
Bme1580I G_kGCm'C
Cuts at: 1704 1797 2853 1704
Size: 93 1056 2930
Fragments arranged by size:
2930 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1372 1707 3851 1291
Size: 81 335 2144 1519
Fragments arranged by size:
2144 1519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 873 2446 873
Size: 1573 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1048
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2134 2626 2924 1255
Size: 879 492 298 2410
Fragments arranged by size:
2410 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3783 3783
Size: 4079
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1268 1789 2467 2741 2888 3299 1061
Size: 207 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 207 147
BseYI C'CCAG_C
Cuts at: 1504 2262 2839 3335 1504
Size: 758 577 496 2248
Fragments arranged by size:
2248 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 655 796 960 242
Size: 413 141 164 3361
Fragments arranged by size:
3361 413 164 141
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1093 1871 2061 2853 1093
Size: 778 190 792 2319
Fragments arranged by size:
2319 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 311 1093 1704 1797 1871 2061 2123 2853 311
Size: 782 611 93 74 190 62 730 1537
Fragments arranged by size:
1537 782 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 775 1129 1343 1645 2026 688
Size: 87 354 214 302 381 2741
Fragments arranged by size:
2741 381 354 302 214 87
BsrBI CCG'CTC
Cuts at: 50 293 332 342 555 2371 2425 3909 4074
Size: 243 39 10 213 1816 54 1484 165
Cuts at: 4074 50
Size: 55
Fragments arranged by size:
1816 1484 243 213 165 55 54 39
10
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4079
BsrFI r'CCGG_y
Cuts at: 139 914 2077 2258 2467 139
Size: 775 1163 181 209 1751
Fragments arranged by size:
1751 1163 775 209 181
BssHII G'CGCG_C
Cuts at: 806 905 806
Size: 99 3980
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 439 929 1456 439
Size: 490 527 3062
Fragments arranged by size:
3062 527 490
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 486 567 486
Size: 81 3998
BstYI r'GATC_y
Cuts at: 1 1104 1571 1594 1929 2175 3187 3273 3285
Size: 1103 467 23 335 246 1012 86 12
Cuts at: 3285 3631 1
Size: 346 449
Fragments arranged by size:
1103 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 479 560 479
Size: 81 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 186 195 230 375 422 943 1322 186
Size: 9 35 145 47 521 379 2943
Fragments arranged by size:
2943 521 379 145 47 35 9
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 452 476 530 717 797 966 1664 1838 2229
Size: 24 54 187 80 169 698 174 391
Cuts at: 2229 2256 3601 452
Size: 27 1345 930
Fragments arranged by size:
1345 930 698 391 187 174 169 80
54 27 24
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 360 772 2102 2312 360
Size: 412 1330 210 2127
Fragments arranged by size:
2127 1330 412 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 416 2287 2597 2743 3889 416
Size: 1871 310 146 1146 606
Fragments arranged by size:
1871 1146 606 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1048 1904 2336 3083 3351 643
Size: 405 856 432 747 268 1371
Fragments arranged by size:
1371 856 747 432 405 268
EcoO109I rG'GnC_Cy
Cuts at: 1218 1218
Size: 4079
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 4079
FspI TGC'GCA
Cuts at: 605 1860 605
Size: 1255 2824
HaeII r_GCGC'y
Cuts at: 746 1513 1761 2783 3762 746
Size: 767 248 1022 979 1063
Fragments arranged by size:
1063 1022 979 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 954 986 1921 1951 1953 1983 954
Size: 32 935 30 2 30 3050
Fragments arranged by size:
3050 935 32 30 30 2
HincII GTy'rAC
Cuts at: 1118 1118
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 262 749 1069 2750 2934 262
Size: 487 320 1681 184 1407
Fragments arranged by size:
1681 1407 487 320 184
MscI TGG'CCA
Cuts at: 454 532 719 968 1840 454
Size: 78 187 249 872 2693
Fragments arranged by size:
2693 872 249 187 78
MslI CAynn'nnrTG
Cuts at: 484 518 616 2068 2195 484
Size: 34 98 1452 127 2368
Fragments arranged by size:
2368 1452 127 98 34
MspA1I CmG'CkG
Cuts at: 173 439 585 651 1021 1190 1504 1864 2877
Size: 266 146 66 370 169 314 360 1013
Cuts at: 2877 3122 173
Size: 245 1130
Fragments arranged by size:
1130 1013 370 360 314 266 245 169
146 66
NaeI GCC'GGC
Cuts at: 141 916 2260 141
Size: 775 1344 1960
Fragments arranged by size:
1960 1344 775
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 479 479
Size: 4079
NgoMIV G'CCGG_C
Cuts at: 139 914 2258 139
Size: 775 1344 1960
Fragments arranged by size:
1960 1344 775
NspI r_CATG'y
Cuts at: 903 1132 1632 2539 903
Size: 229 500 907 2443
Fragments arranged by size:
2443 907 500 229
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PstI C_TGCA'G
Cuts at: 435 596 1126 1811 435
Size: 161 530 685 2703
Fragments arranged by size:
2703 685 530 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 585 1504 1864 585
Size: 919 360 2800
Fragments arranged by size:
2800 919 360
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 431 592 1122 1807 2800 2991 33
Size: 398 161 530 685 993 191 1121
Fragments arranged by size:
1121 993 685 530 398 191 161
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 1234 2149 2641 2903 1234
Size: 915 492 262 2410
Fragments arranged by size:
2410 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StuI AGG'CCT
Cuts at: 764 764
Size: 4079
StyI C'CwwG_G
Cuts at: 479 1213 1567 479
Size: 734 354 2991
Fragments arranged by size:
2991 734 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1662 1795 2254 2463 1662
Size: 133 459 209 3278
Fragments arranged by size:
3278 459 209 133
TatI w'GTAC_w
Cuts at: 3594 3594
Size: 4079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1574 1574
Size: 4079
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI