Restriction map for pF1KB8183
 (Circular) (Six-base) MAPSORT of: pF1KB8183.seq  Check: 8161  from: 1  to: 3725



pF1KB8183  3725 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    763     763 
   Size:       3725
 
Acc65I G'GTAC_C

Cuts at:    741     741 
   Size:       3725
 
AclI AA'CG_TT

Cuts at:   3565    3565 
   Size:       3725
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    530    1550    1982    2729    2997     530 
   Size:       1020     432     747     268    1258

  Fragments arranged by size: 

                1258    1020     747     432     268
 
AflIII A'CryG_T

Cuts at:   1077    2181    3158    1077 
   Size:       1104     977    1644

  Fragments arranged by size: 

                1644    1104     977
 
AgeI A'CCGG_T

Cuts at:   2113    2113 
   Size:       3725
 
AlwNI CAG_nnn'CTG

Cuts at:    375    1030    2597     375 
   Size:        655    1567    1503

  Fragments arranged by size: 

                1567    1503     655
 
ApaI G_GGCC'C

Cuts at:    157     157 
   Size:       3725
 
ApaLI G'TGCA_C

Cuts at:   2495    2495 
   Size:       3725
 
ApoI r'AATT_y

Cuts at:    729    3078    3334     729 
   Size:       2349     256    1120

  Fragments arranged by size: 

                2349    1120     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3725
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3725
 
AvaI C'yCGr_G

Cuts at:    156     205     745     156 
   Size:         49     540    3136

  Fragments arranged by size: 

                3136     540      49
 
BamHI G'GATC_C

Cuts at:    750     750 
   Size:       3725
 
BanI G'GyrC_C

Cuts at:    136     710     741    1155    1403    1438     136 
   Size:        574      31     414     248      35    2423

  Fragments arranged by size: 

                2423     574     414     248      35      31
 
BanII G_rGCy'C

Cuts at:    157     205     739    1769     157 
   Size:         48     534    1030    2113

  Fragments arranged by size: 

                2113    1030     534      48
 
BbeI G_GCGC'C

Cuts at:    140     714    1159    1407     140 
   Size:        574     445     248    2458

  Fragments arranged by size: 

                2458     574     445     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    509     509 
   Size:       3725
 
BciVI GTATCCnnnnn_n'

Cuts at:   1623    2384    3725    1623 
   Size:        761    1341    1623

  Fragments arranged by size: 

                1623    1341     761
 
BclI T'GATC_A

Cuts at:   1245    1245 
   Size:       3725
 
BglII A'GATC_T

Cuts at:   1240    3277    1240 
   Size:       2037    1688
 
BlpI GC'TnA_GC

Cuts at:    837     837 
   Size:       3725
 
Bme1580I G_kGCm'C

Cuts at:    157    1350    1443    2499     157 
   Size:       1193      93    1056    1383

  Fragments arranged by size: 

                1383    1193    1056      93
 
BmgBI CAC'GTC

Cuts at:    507     507 
   Size:       3725
 
BmrI ACTGGGnnnn_n'

Cuts at:    689     937    1018    1353    3497     689 
   Size:        248      81     335    2144     917

  Fragments arranged by size: 

                2144     917     335     248      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    615    2092     615 
   Size:       1477    2248
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    901    1780    2272    2570     901 
   Size:        879     492     298    2056

  Fragments arranged by size: 

                2056     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3429    3429 
   Size:       3725
 
BsaAI yAC'GTr

Cuts at:   1078    1078 
   Size:       3725
 
BsaBI GATnn'nnATC

Cuts at:      6    1239    3276       6 
   Size:       1233    2037     455

  Fragments arranged by size: 

                2037    1233     455
 
BsaHI Gr'CG_yC

Cuts at:    137     711    1156    1404    2106    3602     137 
   Size:        574     445     248     702    1496     260

  Fragments arranged by size: 

                1496     702     574     445     260     248
 
BsaWI w'CCGG_w

Cuts at:    914    1435    2113    2387    2534    2945     914 
   Size:        521     678     274     147     411    1694

  Fragments arranged by size: 

                1694     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    199     493     505     591     199 
   Size:        294      12      86    3333

  Fragments arranged by size: 

                3333     294      86      12
 
BseYI C'CCAG_C

Cuts at:    237    1150    1908    2485    2981     237 
   Size:        913     758     577     496     981

  Fragments arranged by size: 

                 981     913     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1267    1313    2521     104 
   Size:       1163      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1163      46
 
BsiHKAI G_wGCw'C

Cuts at:    355     739    1517    1707    2499     355 
   Size:        384     778     190     792    1581

  Fragments arranged by size: 

                1581     792     778     384     190
 
Bsp1286I G_dGCh'C

Cuts at:    157     205     355     739    1350    1443    1517    1707    1769 
   Size:         48     150     384     611      93      74     190      62

Cuts at:   1769    2499     157 
   Size:        730    1383

  Fragments arranged by size: 

                1383     730     611     384     190     150      93      74
                  62      48
 
BspEI T'CCGG_A

Cuts at:   2945    2945 
   Size:       3725
 
BspHI T'CATG_A

Cuts at:   2901    2901 
   Size:       3725
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    309     775     989    1291    1672     309 
   Size:        466     214     302     381    2362

  Fragments arranged by size: 

                2362     466     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2017    2071    3555    3720      50 
   Size:       1967      54    1484     165      55

  Fragments arranged by size: 

                1967    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1637    1637 
   Size:       3725
 
BsrFI r'CCGG_y

Cuts at:   1723    1904    2113    1723 
   Size:        181     209    3335

  Fragments arranged by size: 

                3335     209     181
 
BssSI C'ACGA_G

Cuts at:   1996    2354    1996 
   Size:        358    3367
 
BstAPI GCAn_nnn'nTGC

Cuts at:    375    1102     375 
   Size:        727    2998
 
BstBI TT'CG_AA

Cuts at:   2086    2086 
   Size:       3725
 
BstEII G'GTnAC_C

Cuts at:    651     651 
   Size:       3725
 
BstYI r'GATC_y

Cuts at:      1     750    1217    1240    1575    1821    2833    2919    2931 
   Size:        749     467      23     335     246    1012      86      12

Cuts at:   2931    3277       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     749     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3725
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1675    1856    2019    1675 
   Size:        181     163    3381

  Fragments arranged by size: 

                3381     181     163
 
BtsI GCAGTG_nn'

Cuts at:    968     968 
   Size:       3725
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    262     297     262 
   Size:         35    3690
 
DraI TTT'AAA

Cuts at:    724     724 
   Size:       3725
 
DrdI GACnn_nn'nnGTC

Cuts at:   1431    2289    1431 
   Size:        858    2867
 
EaeI y'GGCC_r

Cuts at:    167    1310    1484    1875    1902    3247     167 
   Size:       1143     174     391      27    1345     645

  Fragments arranged by size: 

                1345    1143     645     391     174      27
 
EagI C'GGCC_G

Cuts at:   1310    1310 
   Size:       3725
 
EarI CTCTTCn'nnn_

Cuts at:    229     428     470    1748    1958     229 
   Size:        199      42    1278     210    1996

  Fragments arranged by size: 

                1996    1278     210     199      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    196    1933    2243    2389    3535     196 
   Size:       1737     310     146    1146     386

  Fragments arranged by size: 

                1737    1146     386     310     146
 
EcoICRI GAG'CTC

Cuts at:    737     737 
   Size:       3725
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    530    1550    1982    2729    2997     530 
   Size:       1020     432     747     268    1258

  Fragments arranged by size: 

                1258    1020     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    153     321     864     153 
   Size:        168     543    3014

  Fragments arranged by size: 

                3014     543     168
 
EcoRI G'AATT_C

Cuts at:    729     729 
   Size:       3725
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1744    1744 
   Size:       3725
 
FspI TGC'GCA

Cuts at:   1506    1506 
   Size:       3725
 
HaeII r_GCGC'y

Cuts at:    140     376     714    1159    1407    2429    3408     140 
   Size:        236     338     445     248    1022     979     457

  Fragments arranged by size: 

                1022     979     457     445     338     248     236
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1567    1597    1599    1629    1567 
   Size:         30       2      30    3663

  Fragments arranged by size: 

                3663      30      30       2
 
HincII GTy'rAC

Cuts at:    764     764 
   Size:       3725
 
KasI G'GCGC_C

Cuts at:    136     710    1155    1403     136 
   Size:        574     445     248    2458

  Fragments arranged by size: 

                2458     574     445     248
 
KpnI G_GTAC'C

Cuts at:    745     745 
   Size:       3725
 
MluI A'CGCG_T

Cuts at:   3158    3158 
   Size:       3725
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2396    2580    2396 
   Size:        184    3541
 
MscI TGG'CCA

Cuts at:   1486    1486 
   Size:       3725
 
MslI CAynn'nnrTG

Cuts at:   1714    1841    1714 
   Size:        127    3598
 
MspA1I CmG'CkG

Cuts at:    836    1150    1510    2523    2768     836 
   Size:        314     360    1013     245    1793

  Fragments arranged by size: 

                1793    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1906    1906 
   Size:       3725
 
NarI GG'CG_CC

Cuts at:    137     711    1156    1404     137 
   Size:        574     445     248    2458

  Fragments arranged by size: 

                2458     574     445     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3725
 
NgoMIV G'CCGG_C

Cuts at:   1904    1904 
   Size:       3725
 
NspI r_CATG'y

Cuts at:    369     778    1278    2185     369 
   Size:        409     500     907    1909

  Fragments arranged by size: 

                1909     907     500     409
 
PciI A'CATG_T

Cuts at:   2181    2181 
   Size:       3725
 
PmeI GTTT'AAAC

Cuts at:    724     724 
   Size:       3725
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2864    2896    2864 
   Size:         32    3693
 
PpuMI rG'GwC_Cy

Cuts at:    321     321 
   Size:       3725
 
PspOMI G'GGCC_C

Cuts at:    153     153 
   Size:       3725
 
PstI C_TGCA'G

Cuts at:    772    1457     772 
   Size:        685    3040
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3725
 
PvuII CAG'CTG

Cuts at:   1150    1510    1150 
   Size:        360    3365
 
RsrII CG'GwC_CG

Cuts at:   1920    1920 
   Size:       3725
 
SacI G_AGCT'C

Cuts at:    739     739 
   Size:       3725
 
SalI G'TCGA_C

Cuts at:    762     762 
   Size:       3725
 
SapI GCTCTTCn'nnn_

Cuts at:   1748    1958    1748 
   Size:        210    3515
 
SbfI CC_TGCA'GG

Cuts at:    772     772 
   Size:       3725
 
ScaI AGT'ACT

Cuts at:   3242    3242 
   Size:       3725
 
SfcI C'TryA_G

Cuts at:     33     768    1453    2446    2637      33 
   Size:        735     685     993     191    1121

  Fragments arranged by size: 

                1121     993     735     685     191
 
SfoI GGC'GCC

Cuts at:    138     712    1157    1405     138 
   Size:        574     445     248    2458

  Fragments arranged by size: 

                2458     574     445     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3725
 
SmaI CCC'GGG

Cuts at:    158     747     158 
   Size:        589    3136
 
SmlI C'TyrA_G

Cuts at:    880    1795    2287    2549     880 
   Size:        915     492     262    2056

  Fragments arranged by size: 

                2056     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3725
 
SphI G_CATG'C

Cuts at:    778    1278     778 
   Size:        500    3225
 
StyI C'CwwG_G

Cuts at:    107     329     859    1213     107 
   Size:        222     530     354    2619

  Fragments arranged by size: 

                2619     530     354     222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1308    1441    1900    2109    1308 
   Size:        133     459     209    2924

  Fragments arranged by size: 

                2924     459     209     133
 
TatI w'GTAC_w

Cuts at:   3240    3240 
   Size:       3725
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3242    3274    3242 
   Size:         32    3693
 
Tth111I GACn'n_nGTC

Cuts at:    661    1522     661 
   Size:        861    2864
 
XbaI T'CTAG_A

Cuts at:    756     756 
   Size:       3725
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1220    1220 
   Size:       3725
 
XmaI C'CCGG_G

Cuts at:    156     745     156 
   Size:        589    3136
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI     BmtI
     BpmI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII    BstXI  BstZ17I   Bsu36I     ClaI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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