(Circular) (Six-base) MAPSORT of: pF1KB8183.seq Check: 8161 from: 1 to: 3725 pF1KB8183 3725 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 763 763 Size: 3725 Acc65I G'GTAC_C Cuts at: 741 741 Size: 3725 AclI AA'CG_TT Cuts at: 3565 3565 Size: 3725 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 530 1550 1982 2729 2997 530 Size: 1020 432 747 268 1258 Fragments arranged by size: 1258 1020 747 432 268 AflIII A'CryG_T Cuts at: 1077 2181 3158 1077 Size: 1104 977 1644 Fragments arranged by size: 1644 1104 977 AgeI A'CCGG_T Cuts at: 2113 2113 Size: 3725 AlwNI CAG_nnn'CTG Cuts at: 375 1030 2597 375 Size: 655 1567 1503 Fragments arranged by size: 1567 1503 655 ApaI G_GGCC'C Cuts at: 157 157 Size: 3725 ApaLI G'TGCA_C Cuts at: 2495 2495 Size: 3725 ApoI r'AATT_y Cuts at: 729 3078 3334 729 Size: 2349 256 1120 Fragments arranged by size: 2349 1120 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3725 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3725 AvaI C'yCGr_G Cuts at: 156 205 745 156 Size: 49 540 3136 Fragments arranged by size: 3136 540 49 BamHI G'GATC_C Cuts at: 750 750 Size: 3725 BanI G'GyrC_C Cuts at: 136 710 741 1155 1403 1438 136 Size: 574 31 414 248 35 2423 Fragments arranged by size: 2423 574 414 248 35 31 BanII G_rGCy'C Cuts at: 157 205 739 1769 157 Size: 48 534 1030 2113 Fragments arranged by size: 2113 1030 534 48 BbeI G_GCGC'C Cuts at: 140 714 1159 1407 140 Size: 574 445 248 2458 Fragments arranged by size: 2458 574 445 248 BbsI GAAGACnn'nnnn_ Cuts at: 509 509 Size: 3725 BciVI GTATCCnnnnn_n' Cuts at: 1623 2384 3725 1623 Size: 761 1341 1623 Fragments arranged by size: 1623 1341 761 BclI T'GATC_A Cuts at: 1245 1245 Size: 3725 BglII A'GATC_T Cuts at: 1240 3277 1240 Size: 2037 1688 BlpI GC'TnA_GC Cuts at: 837 837 Size: 3725 Bme1580I G_kGCm'C Cuts at: 157 1350 1443 2499 157 Size: 1193 93 1056 1383 Fragments arranged by size: 1383 1193 1056 93 BmgBI CAC'GTC Cuts at: 507 507 Size: 3725 BmrI ACTGGGnnnn_n' Cuts at: 689 937 1018 1353 3497 689 Size: 248 81 335 2144 917 Fragments arranged by size: 2144 917 335 248 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 615 2092 615 Size: 1477 2248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 901 1780 2272 2570 901 Size: 879 492 298 2056 Fragments arranged by size: 2056 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3429 3429 Size: 3725 BsaAI yAC'GTr Cuts at: 1078 1078 Size: 3725 BsaBI GATnn'nnATC Cuts at: 6 1239 3276 6 Size: 1233 2037 455 Fragments arranged by size: 2037 1233 455 BsaHI Gr'CG_yC Cuts at: 137 711 1156 1404 2106 3602 137 Size: 574 445 248 702 1496 260 Fragments arranged by size: 1496 702 574 445 260 248 BsaWI w'CCGG_w Cuts at: 914 1435 2113 2387 2534 2945 914 Size: 521 678 274 147 411 1694 Fragments arranged by size: 1694 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 199 493 505 591 199 Size: 294 12 86 3333 Fragments arranged by size: 3333 294 86 12 BseYI C'CCAG_C Cuts at: 237 1150 1908 2485 2981 237 Size: 913 758 577 496 981 Fragments arranged by size: 981 913 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1267 1313 2521 104 Size: 1163 46 1208 1308 Fragments arranged by size: 1308 1208 1163 46 BsiHKAI G_wGCw'C Cuts at: 355 739 1517 1707 2499 355 Size: 384 778 190 792 1581 Fragments arranged by size: 1581 792 778 384 190 Bsp1286I G_dGCh'C Cuts at: 157 205 355 739 1350 1443 1517 1707 1769 Size: 48 150 384 611 93 74 190 62 Cuts at: 1769 2499 157 Size: 730 1383 Fragments arranged by size: 1383 730 611 384 190 150 93 74 62 48 BspEI T'CCGG_A Cuts at: 2945 2945 Size: 3725 BspHI T'CATG_A Cuts at: 2901 2901 Size: 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 309 775 989 1291 1672 309 Size: 466 214 302 381 2362 Fragments arranged by size: 2362 466 381 302 214 BsrBI CCG'CTC Cuts at: 50 2017 2071 3555 3720 50 Size: 1967 54 1484 165 55 Fragments arranged by size: 1967 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1637 1637 Size: 3725 BsrFI r'CCGG_y Cuts at: 1723 1904 2113 1723 Size: 181 209 3335 Fragments arranged by size: 3335 209 181 BssSI C'ACGA_G Cuts at: 1996 2354 1996 Size: 358 3367 BstAPI GCAn_nnn'nTGC Cuts at: 375 1102 375 Size: 727 2998 BstBI TT'CG_AA Cuts at: 2086 2086 Size: 3725 BstEII G'GTnAC_C Cuts at: 651 651 Size: 3725 BstYI r'GATC_y Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931 Size: 749 467 23 335 246 1012 86 12 Cuts at: 2931 3277 1 Size: 346 449 Fragments arranged by size: 1012 749 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3725 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1675 1856 2019 1675 Size: 181 163 3381 Fragments arranged by size: 3381 181 163 BtsI GCAGTG_nn' Cuts at: 968 968 Size: 3725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 262 297 262 Size: 35 3690 DraI TTT'AAA Cuts at: 724 724 Size: 3725 DrdI GACnn_nn'nnGTC Cuts at: 1431 2289 1431 Size: 858 2867 EaeI y'GGCC_r Cuts at: 167 1310 1484 1875 1902 3247 167 Size: 1143 174 391 27 1345 645 Fragments arranged by size: 1345 1143 645 391 174 27 EagI C'GGCC_G Cuts at: 1310 1310 Size: 3725 EarI CTCTTCn'nnn_ Cuts at: 229 428 470 1748 1958 229 Size: 199 42 1278 210 1996 Fragments arranged by size: 1996 1278 210 199 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 196 1933 2243 2389 3535 196 Size: 1737 310 146 1146 386 Fragments arranged by size: 1737 1146 386 310 146 EcoICRI GAG'CTC Cuts at: 737 737 Size: 3725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 530 1550 1982 2729 2997 530 Size: 1020 432 747 268 1258 Fragments arranged by size: 1258 1020 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 153 321 864 153 Size: 168 543 3014 Fragments arranged by size: 3014 543 168 EcoRI G'AATT_C Cuts at: 729 729 Size: 3725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1744 1744 Size: 3725 FspI TGC'GCA Cuts at: 1506 1506 Size: 3725 HaeII r_GCGC'y Cuts at: 140 376 714 1159 1407 2429 3408 140 Size: 236 338 445 248 1022 979 457 Fragments arranged by size: 1022 979 457 445 338 248 236 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1567 1597 1599 1629 1567 Size: 30 2 30 3663 Fragments arranged by size: 3663 30 30 2 HincII GTy'rAC Cuts at: 764 764 Size: 3725 KasI G'GCGC_C Cuts at: 136 710 1155 1403 136 Size: 574 445 248 2458 Fragments arranged by size: 2458 574 445 248 KpnI G_GTAC'C Cuts at: 745 745 Size: 3725 MluI A'CGCG_T Cuts at: 3158 3158 Size: 3725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2396 2580 2396 Size: 184 3541 MscI TGG'CCA Cuts at: 1486 1486 Size: 3725 MslI CAynn'nnrTG Cuts at: 1714 1841 1714 Size: 127 3598 MspA1I CmG'CkG Cuts at: 836 1150 1510 2523 2768 836 Size: 314 360 1013 245 1793 Fragments arranged by size: 1793 1013 360 314 245 NaeI GCC'GGC Cuts at: 1906 1906 Size: 3725 NarI GG'CG_CC Cuts at: 137 711 1156 1404 137 Size: 574 445 248 2458 Fragments arranged by size: 2458 574 445 248 NcoI C'CATG_G Cuts at: 107 107 Size: 3725 NgoMIV G'CCGG_C Cuts at: 1904 1904 Size: 3725 NspI r_CATG'y Cuts at: 369 778 1278 2185 369 Size: 409 500 907 1909 Fragments arranged by size: 1909 907 500 409 PciI A'CATG_T Cuts at: 2181 2181 Size: 3725 PmeI GTTT'AAAC Cuts at: 724 724 Size: 3725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2864 2896 2864 Size: 32 3693 PpuMI rG'GwC_Cy Cuts at: 321 321 Size: 3725 PspOMI G'GGCC_C Cuts at: 153 153 Size: 3725 PstI C_TGCA'G Cuts at: 772 1457 772 Size: 685 3040 PvuI CG_AT'CG Cuts at: 104 104 Size: 3725 PvuII CAG'CTG Cuts at: 1150 1510 1150 Size: 360 3365 RsrII CG'GwC_CG Cuts at: 1920 1920 Size: 3725 SacI G_AGCT'C Cuts at: 739 739 Size: 3725 SalI G'TCGA_C Cuts at: 762 762 Size: 3725 SapI GCTCTTCn'nnn_ Cuts at: 1748 1958 1748 Size: 210 3515 SbfI CC_TGCA'GG Cuts at: 772 772 Size: 3725 ScaI AGT'ACT Cuts at: 3242 3242 Size: 3725 SfcI C'TryA_G Cuts at: 33 768 1453 2446 2637 33 Size: 735 685 993 191 1121 Fragments arranged by size: 1121 993 735 685 191 SfoI GGC'GCC Cuts at: 138 712 1157 1405 138 Size: 574 445 248 2458 Fragments arranged by size: 2458 574 445 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3725 SmaI CCC'GGG Cuts at: 158 747 158 Size: 589 3136 SmlI C'TyrA_G Cuts at: 880 1795 2287 2549 880 Size: 915 492 262 2056 Fragments arranged by size: 2056 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3725 SphI G_CATG'C Cuts at: 778 1278 778 Size: 500 3225 StyI C'CwwG_G Cuts at: 107 329 859 1213 107 Size: 222 530 354 2619 Fragments arranged by size: 2619 530 354 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1308 1441 1900 2109 1308 Size: 133 459 209 2924 Fragments arranged by size: 2924 459 209 133 TatI w'GTAC_w Cuts at: 3240 3240 Size: 3725 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3242 3274 3242 Size: 32 3693 Tth111I GACn'n_nGTC Cuts at: 661 1522 661 Size: 861 2864 XbaI T'CTAG_A Cuts at: 756 756 Size: 3725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1220 1220 Size: 3725 XmaI C'CCGG_G Cuts at: 156 745 156 Size: 589 3136 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI