Restriction map for
pF1KB8182
(Circular) (Six-base) MAPSORT of: pF1KB8182.seq Check: 6152 from: 1 to: 3809
pF1KB8182 3809 bp
With 182 enzymes: SgfI *
June 16, 2009 11:53 ..
AccI GT'mk_AC
Cuts at: 847 847
Size: 3809
Acc65I G'GTAC_C
Cuts at: 825 825
Size: 3809
AclI AA'CG_TT
Cuts at: 3649 3649
Size: 3809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 679 1634 2066 2813 3081 644
Size: 35 955 432 747 268 1372
Fragments arranged by size:
1372 955 747 432 268 35
AflIII A'CryG_T
Cuts at: 1161 2265 3242 1161
Size: 1104 977 1728
Fragments arranged by size:
1728 1104 977
AgeI A'CCGG_T
Cuts at: 2197 2197
Size: 3809
AleI CACnn'nnGTG
Cuts at: 172 172
Size: 3809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 657 625
Size: 32 3777
AlwNI CAG_nnn'CTG
Cuts at: 280 1114 2681 280
Size: 834 1567 1408
Fragments arranged by size:
1567 1408 834
ApaLI G'TGCA_C
Cuts at: 2579 2579
Size: 3809
ApoI r'AATT_y
Cuts at: 689 813 3162 3418 689
Size: 124 2349 256 1080
Fragments arranged by size:
2349 1080 256 124
AseI AT'TA_AT
Cuts at: 20 20
Size: 3809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
AvaI C'yCGr_G
Cuts at: 829 829
Size: 3809
BamHI G'GATC_C
Cuts at: 834 834
Size: 3809
BanI G'GyrC_C
Cuts at: 268 825 1239 1487 1522 268
Size: 557 414 248 35 2555
Fragments arranged by size:
2555 557 414 248 35
BanII G_rGCy'C
Cuts at: 580 823 1853 580
Size: 243 1030 2536
Fragments arranged by size:
2536 1030 243
BbeI G_GCGC'C
Cuts at: 1243 1491 1243
Size: 248 3561
BbsI GAAGACnn'nnnn_
Cuts at: 491 491
Size: 3809
BbvCI CC'TCA_GC
Cuts at: 308 308
Size: 3809
BciVI GTATCCnnnnn_n'
Cuts at: 1707 2468 3809 1707
Size: 761 1341 1707
Fragments arranged by size:
1707 1341 761
BclI T'GATC_A
Cuts at: 1329 1329
Size: 3809
BfrBI ATG'CAT
Cuts at: 538 538
Size: 3809
BglII A'GATC_T
Cuts at: 1324 3361 1324
Size: 2037 1772
BlpI GC'TnA_GC
Cuts at: 921 921
Size: 3809
Bme1580I G_kGCm'C
Cuts at: 1434 1527 2583 1434
Size: 93 1056 2660
Fragments arranged by size:
2660 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 744 1021 1102 1437 3581 744
Size: 277 81 335 2144 972
Fragments arranged by size:
2144 972 335 277 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 594 2176 594
Size: 1582 2227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 586
Size: 3809
Bpu10I CC'TnA_GC
Cuts at: 308 461 308
Size: 153 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 985 1864 2356 2654 811
Size: 174 879 492 298 1966
Fragments arranged by size:
1966 879 492 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 3513 3513
Size: 3809
BsaAI yAC'GTr
Cuts at: 1162 1162
Size: 3809
BsaBI GATnn'nnATC
Cuts at: 6 1323 3360 6
Size: 1317 2037 455
Fragments arranged by size:
2037 1317 455
BsaHI Gr'CG_yC
Cuts at: 1240 1488 2190 3686 1240
Size: 248 702 1496 1363
Fragments arranged by size:
1496 1363 702 248
BsaWI w'CCGG_w
Cuts at: 998 1519 2197 2471 2618 3029 998
Size: 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 463 583 588 815 325
Size: 138 120 5 227 3319
Fragments arranged by size:
3319 227 138 120 5
BseYI C'CCAG_C
Cuts at: 1234 1992 2569 3065 1234
Size: 758 577 496 1978
Fragments arranged by size:
1978 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1351 1397 2605 104
Size: 1247 46 1208 1308
Fragments arranged by size:
1308 1247 1208 46
BsiHKAI G_wGCw'C
Cuts at: 580 823 1601 1791 2583 580
Size: 243 778 190 792 1806
Fragments arranged by size:
1806 792 778 243 190
BsmI GAATG_Cn'
Cuts at: 538 538
Size: 3809
Bsp1286I G_dGCh'C
Cuts at: 580 823 1434 1527 1601 1791 1853 2583 580
Size: 243 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 730 611 243 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3029 3029
Size: 3809
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 859 1073 1375 1756 859
Size: 214 302 381 2912
Fragments arranged by size:
2912 381 302 214
BsrBI CCG'CTC
Cuts at: 50 739 2101 2155 3639 3804 50
Size: 689 1362 54 1484 165 55
Fragments arranged by size:
1484 1362 689 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1721 1721
Size: 3809
BsrFI r'CCGG_y
Cuts at: 1807 1988 2197 1807
Size: 181 209 3419
Fragments arranged by size:
3419 209 181
BssSI C'ACGA_G
Cuts at: 2080 2438 2080
Size: 358 3451
BstAPI GCAn_nnn'nTGC
Cuts at: 1186 1186
Size: 3809
BstBI TT'CG_AA
Cuts at: 238 2170 238
Size: 1932 1877
BstEII G'GTnAC_C
Cuts at: 135 135
Size: 3809
BstXI CCAn_nnnn'nTGG
Cuts at: 378 378
Size: 3809
BstYI r'GATC_y
Cuts at: 1 834 1301 1324 1659 1905 2917 3003 3015
Size: 833 467 23 335 246 1012 86 12
Cuts at: 3015 3361 1
Size: 346 449
Fragments arranged by size:
1012 833 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 170 107
Size: 63 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1759 1940 2103 1759
Size: 181 163 3465
Fragments arranged by size:
3465 181 163
BtsI GCAGTG_nn'
Cuts at: 1052 1052
Size: 3809
DraI TTT'AAA
Cuts at: 808 808
Size: 3809
DrdI GACnn_nn'nnGTC
Cuts at: 1515 2373 1515
Size: 858 2951
EaeI y'GGCC_r
Cuts at: 743 1394 1568 1959 1986 3331 743
Size: 651 174 391 27 1345 1221
Fragments arranged by size:
1345 1221 651 391 174 27
EagI C'GGCC_G
Cuts at: 1394 1394
Size: 3809
EarI CTCTTCn'nnn_
Cuts at: 294 500 620 1832 2042 294
Size: 206 120 1212 210 2061
Fragments arranged by size:
2061 1212 210 206 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 2017 2327 2473 3619 163
Size: 1854 310 146 1146 353
Fragments arranged by size:
1854 1146 353 310 146
EcoICRI GAG'CTC
Cuts at: 578 821 578
Size: 243 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 644 679 1634 2066 2813 3081 586
Size: 58 35 955 432 747 268 1314
Fragments arranged by size:
1314 955 747 432 268 58 35
EcoO109I rG'GnC_Cy
Cuts at: 948 948
Size: 3809
EcoRI G'AATT_C
Cuts at: 813 813
Size: 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3809
FspI TGC'GCA
Cuts at: 1590 1590
Size: 3809
HaeII r_GCGC'y
Cuts at: 1243 1491 2513 3492 1243
Size: 248 1022 979 1560
Fragments arranged by size:
1560 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1651 1681 1683 1713 1651
Size: 30 2 30 3747
Fragments arranged by size:
3747 30 30 2
HincII GTy'rAC
Cuts at: 413 848 413
Size: 435 3374
HpaI GTT'AAC
Cuts at: 413 413
Size: 3809
KasI G'GCGC_C
Cuts at: 1239 1487 1239
Size: 248 3561
KpnI G_GTAC'C
Cuts at: 829 829
Size: 3809
MluI A'CGCG_T
Cuts at: 3242 3242
Size: 3809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2664 2480
Size: 184 3625
MscI TGG'CCA
Cuts at: 745 1570 745
Size: 825 2984
MslI CAynn'nnrTG
Cuts at: 169 172 1798 1925 169
Size: 3 1626 127 2053
Fragments arranged by size:
2053 1626 127 3
MspA1I CmG'CkG
Cuts at: 249 501 555 920 1234 1594 2607 2852 249
Size: 252 54 365 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 365 360 314 252 245 54
NaeI GCC'GGC
Cuts at: 1990 1990
Size: 3809
NarI GG'CG_CC
Cuts at: 1240 1488 1240
Size: 248 3561
NcoI C'CATG_G
Cuts at: 107 107
Size: 3809
NgoMIV G'CCGG_C
Cuts at: 1988 1988
Size: 3809
NsiI A_TGCA'T
Cuts at: 540 540
Size: 3809
NspI r_CATG'y
Cuts at: 789 862 1362 2269 789
Size: 73 500 907 2329
Fragments arranged by size:
2329 907 500 73
PciI A'CATG_T
Cuts at: 2265 2265
Size: 3809
PmeI GTTT'AAAC
Cuts at: 808 808
Size: 3809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3777
PstI C_TGCA'G
Cuts at: 683 856 1541 683
Size: 173 685 2951
Fragments arranged by size:
2951 685 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3809
PvuII CAG'CTG
Cuts at: 501 555 1234 1594 501
Size: 54 679 360 2716
Fragments arranged by size:
2716 679 360 54
RsrII CG'GwC_CG
Cuts at: 2004 2004
Size: 3809
SacI G_AGCT'C
Cuts at: 580 823 580
Size: 243 3566
SalI G'TCGA_C
Cuts at: 846 846
Size: 3809
SapI GCTCTTCn'nnn_
Cuts at: 1832 2042 1832
Size: 210 3599
SbfI CC_TGCA'GG
Cuts at: 856 856
Size: 3809
ScaI AGT'ACT
Cuts at: 3326 3326
Size: 3809
SfcI C'TryA_G
Cuts at: 33 588 679 852 1537 2530 2721 33
Size: 555 91 173 685 993 191 1121
Fragments arranged by size:
1121 993 685 555 191 173 91
SfoI GGC'GCC
Cuts at: 1241 1489 1241
Size: 248 3561
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
SmaI CCC'GGG
Cuts at: 831 831
Size: 3809
SmlI C'TyrA_G
Cuts at: 790 964 1879 2371 2633 790
Size: 174 915 492 262 1966
Fragments arranged by size:
1966 915 492 262 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3809
SphI G_CATG'C
Cuts at: 789 862 1362 789
Size: 73 500 3236
Fragments arranged by size:
3236 500 73
StyI C'CwwG_G
Cuts at: 107 943 1297 107
Size: 836 354 2619
Fragments arranged by size:
2619 836 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 398 530 730 763 1392 1525 1984 2193
Size: 298 132 200 33 629 133 459 209
Cuts at: 2193 100
Size: 1716
Fragments arranged by size:
1716 629 459 298 209 200 133 132
33
TatI w'GTAC_w
Cuts at: 3324 3324
Size: 3809
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 3777
Tth111I GACn'n_nGTC
Cuts at: 1606 1606
Size: 3809
XbaI T'CTAG_A
Cuts at: 840 840
Size: 3809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1304
Size: 3809
XmaI C'CCGG_G
Cuts at: 829 829
Size: 3809
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI