Restriction map for
pF1KB8180
(Circular) (Six-base) MAPSORT of: pF1KB8180.seq Check: 8097 from: 1 to: 4388
pF1KB8180 4388 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1115 1426 1115
Size: 311 4077
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 1285 2213 2645 3392 3660 983
Size: 302 928 432 747 268 1711
Fragments arranged by size:
1711 928 747 432 302 268
AfeI AGC'GCT
Cuts at: 1161 1161
Size: 4388
AflII C'TTAA_G
Cuts at: 1066 1066
Size: 4388
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AleI CACnn'nnGTG
Cuts at: 1372 1372
Size: 4388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 685 688 995 685
Size: 3 307 4078
Fragments arranged by size:
4078 307 3
AlwNI CAG_nnn'CTG
Cuts at: 921 1693 3260 921
Size: 772 1567 2049
Fragments arranged by size:
2049 1567 772
ApaI G_GGCC'C
Cuts at: 591 948 591
Size: 357 4031
ApaLI G'TGCA_C
Cuts at: 980 3158 980
Size: 2178 2210
ApoI r'AATT_y
Cuts at: 1198 1392 3741 3997 1198
Size: 194 2349 256 1589
Fragments arranged by size:
2349 1589 256 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 941 1408 941
Size: 467 3921
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 636 853 1404 1818 2066 2101 636
Size: 217 551 414 248 35 2923
Fragments arranged by size:
2923 551 414 248 217 35
BanII G_rGCy'C
Cuts at: 116 135 591 935 948 1402 2432 116
Size: 19 456 344 13 454 1030 2072
Fragments arranged by size:
2072 1030 456 454 344 19 13
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 997 1017 997
Size: 20 4368
BbvCI CC'TCA_GC
Cuts at: 117 861 117
Size: 744 3644
BciVI GTATCCnnnnn_n'
Cuts at: 2286 3047 4388 2286
Size: 761 1341 2286
Fragments arranged by size:
2286 1341 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglI GCCn_nnn'nGGC
Cuts at: 268 586 958 1241 268
Size: 318 372 283 3415
Fragments arranged by size:
3415 372 318 283
BglII A'GATC_T
Cuts at: 746 1903 3940 746
Size: 1157 2037 1194
Fragments arranged by size:
2037 1194 1157
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 591 641 948 984 2013 2106 3162 591
Size: 50 307 36 1029 93 1056 1817
Fragments arranged by size:
1817 1056 1029 307 93 50 36
BmrI ACTGGGnnnn_n'
Cuts at: 799 1600 1681 2016 4160 799
Size: 801 81 335 2144 1027
Fragments arranged by size:
2144 1027 801 335 81
BmtI G_CTAG'C
Cuts at: 685 685
Size: 4388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 931 164
Size: 767 3621
Bpu10I CC'TnA_GC
Cuts at: 117 677 861 117
Size: 560 184 3644
Fragments arranged by size:
3644 560 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1564 2443 2935 3233 1564
Size: 879 492 298 2719
Fragments arranged by size:
2719 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1275 1275
Size: 4388
BseYI C'CCAG_C
Cuts at: 722 900 935 1813 2571 3148 3644 722
Size: 178 35 878 758 577 496 1466
Fragments arranged by size:
1466 878 758 577 496 178 35
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 577 984 1358 1402 2180 2370 3162 577
Size: 407 374 44 778 190 792 1803
Fragments arranged by size:
1803 792 778 407 374 190 44
BsmI GAATG_Cn'
Cuts at: 664 664
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 116 135 577 591 641 935 948 984 1358
Size: 19 442 14 50 294 13 36 374
Cuts at: 1358 1402 2013 2106 2180 2370 2432 3162 116
Size: 44 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 442 374 294 190 93
74 62 50 44 36 19 14 13
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1049 1438 1652 1954 2335 1049
Size: 389 214 302 381 3102
Fragments arranged by size:
3102 389 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1283 2680 2734 4218 4383 50
Size: 1233 1397 54 1484 165 55
Fragments arranged by size:
1484 1397 1233 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 408 2386 2567 2776 408
Size: 1978 181 209 2020
Fragments arranged by size:
2020 1978 209 181
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 1353 1765 1353
Size: 412 3976
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 420 420
Size: 4388
BstYI r'GATC_y
Cuts at: 1 746 845 1413 1880 1903 2238 2484 3496
Size: 745 99 568 467 23 335 246 1012
Cuts at: 3496 3582 3594 3940 1
Size: 86 12 346 449
Fragments arranged by size:
1012 745 568 467 449 346 335 246
99 86 23 12
BtgI C'CryG_G
Cuts at: 107 591 803 107
Size: 484 212 3692
Fragments arranged by size:
3692 484 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 240 510 2338 2519 2682 240
Size: 270 1828 181 163 1946
Fragments arranged by size:
1946 1828 270 181 163
BtsI GCAGTG_nn'
Cuts at: 512 672 1631 512
Size: 160 959 3269
Fragments arranged by size:
3269 959 160
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 314 410 450 626 800 1973 2147 2538 2565
Size: 96 40 176 174 1173 174 391 27
Cuts at: 2565 3910 314
Size: 1345 792
Fragments arranged by size:
1345 1173 792 391 176 174 174 96
40 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 706 2411 2621 706
Size: 1705 210 2473
Fragments arranged by size:
2473 1705 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 339 1078 2596 2906 3052 4198 339
Size: 739 1518 310 146 1146 529
Fragments arranged by size:
1518 1146 739 529 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 931 983 1285 2213 2645 3392 3660 164
Size: 767 52 302 928 432 747 268 892
Fragments arranged by size:
928 892 767 747 432 302 268 52
EcoNI CCTnn'n_nnAGG
Cuts at: 463 913 463
Size: 450 3938
EcoO109I rG'GnC_Cy
Cuts at: 144 249 906 918 1249 1269 1527 144
Size: 105 657 12 331 20 258 3005
Fragments arranged by size:
3005 657 331 258 105 20 12
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1239 2407 1239
Size: 1168 3220
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 888 1163 1822 2070 3092 4071 888
Size: 275 659 248 1022 979 1205
Fragments arranged by size:
1205 1022 979 659 275 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1313 1345 2230 2260 2262 2292 1313
Size: 32 885 30 2 30 3409
Fragments arranged by size:
3409 885 32 30 30 2
HincII GTy'rAC
Cuts at: 366 1427 366
Size: 1061 3327
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3059 3243 3059
Size: 184 4204
MscI TGG'CCA
Cuts at: 628 802 2149 628
Size: 174 1347 2867
Fragments arranged by size:
2867 1347 174
MslI CAynn'nnrTG
Cuts at: 634 802 1372 2377 2504 634
Size: 168 570 1005 127 2518
Fragments arranged by size:
2518 1005 570 168 127
MspA1I CmG'CkG
Cuts at: 539 900 1499 1813 2173 3186 3431 539
Size: 361 599 314 360 1013 245 1496
Fragments arranged by size:
1496 1013 599 361 360 314 245
NaeI GCC'GGC
Cuts at: 410 2569 410
Size: 2159 2229
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 803 107
Size: 696 3692
NgoMIV G'CCGG_C
Cuts at: 408 2567 408
Size: 2159 2229
NheI G'CTAG_C
Cuts at: 681 681
Size: 4388
NspI r_CATG'y
Cuts at: 1351 1441 1941 2848 1351
Size: 90 500 907 2891
Fragments arranged by size:
2891 907 500 90
PasI CC'CwG_GG
Cuts at: 127 127
Size: 4388
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PflMI CCAn_nnn'nTGG
Cuts at: 1143 1143
Size: 4388
PfoI T'CCnGG_A
Cuts at: 1202 1202
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PpuMI rG'GwC_Cy
Cuts at: 144 249 906 1249 144
Size: 105 657 343 3283
Fragments arranged by size:
3283 657 343 105
PspOMI G'GGCC_C
Cuts at: 587 944 587
Size: 357 4031
PstI C_TGCA'G
Cuts at: 651 1435 2120 651
Size: 784 685 2919
Fragments arranged by size:
2919 784 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 900 1813 2173 900
Size: 913 360 3115
Fragments arranged by size:
3115 913 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SanDI GG'GwC_CC
Cuts at: 144 1249 144
Size: 1105 3283
SapI GCTCTTCn'nnn_
Cuts at: 706 2411 2621 706
Size: 1705 210 2473
Fragments arranged by size:
2473 1705 210
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 481 647 1431 2116 3109 3300 33
Size: 448 166 784 685 993 191 1121
Fragments arranged by size:
1121 993 784 685 448 191 166
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 943 1410 943
Size: 467 3921
SmlI C'TyrA_G
Cuts at: 1066 1543 2458 2950 3212 1066
Size: 477 915 492 262 2242
Fragments arranged by size:
2242 915 492 477 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
StyI C'CwwG_G
Cuts at: 107 803 1522 1876 107
Size: 696 719 354 2619
Fragments arranged by size:
2619 719 696 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 572 1971 2104 2563 2772 572
Size: 1399 133 459 209 2188
Fragments arranged by size:
2188 1399 459 209 133
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4356
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 420 864 1005 1883 420
Size: 444 141 878 2925
Fragments arranged by size:
2925 878 444 141
XmaI C'CCGG_G
Cuts at: 941 1408 941
Size: 467 3921
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI