Restriction map for
pF1KB8178
(Circular) (Six-base) MAPSORT of: pF1KB8178.seq Check: 7804 from: 1 to: 4418
pF1KB8178 4418 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1213 1456 1213
Size: 243 4175
Acc65I G'GTAC_C
Cuts at: 1051 1434 1051
Size: 383 4035
AclI AA'CG_TT
Cuts at: 843 4258 843
Size: 3415 1003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2675 3422 3690 2243
Size: 432 747 268 2971
Fragments arranged by size:
2971 747 432 268
AflIII A'CryG_T
Cuts at: 885 1208 1770 2874 3851 885
Size: 323 562 1104 977 1452
Fragments arranged by size:
1452 1104 977 562 323
AgeI A'CCGG_T
Cuts at: 2806 2806
Size: 4418
AhdI GACnn_n'nnGTC
Cuts at: 544 1302 544
Size: 758 3660
AlwNI CAG_nnn'CTG
Cuts at: 147 472 684 742 1723 3290 147
Size: 325 212 58 981 1567 1275
Fragments arranged by size:
1567 1275 981 325 212 58
ApaLI G'TGCA_C
Cuts at: 3188 3188
Size: 4418
ApoI r'AATT_y
Cuts at: 163 486 1177 1422 3771 4027 163
Size: 323 691 245 2349 256 554
Fragments arranged by size:
2349 691 554 323 256 245
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 1438 1438
Size: 4418
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1035 1068 1035
Size: 33 4385
BamHI G'GATC_C
Cuts at: 895 1443 895
Size: 548 3870
BanI G'GyrC_C
Cuts at: 1051 1434 1848 2096 2131 1051
Size: 383 414 248 35 3338
Fragments arranged by size:
3338 414 383 248 35
BanII G_rGCy'C
Cuts at: 1379 1432 2462 1379
Size: 53 1030 3335
Fragments arranged by size:
3335 1030 53
BbeI G_GCGC'C
Cuts at: 1852 2100 1852
Size: 248 4170
BciVI GTATCCnnnnn_n'
Cuts at: 1389 2316 3077 4418 1389
Size: 927 761 1341 1389
Fragments arranged by size:
1389 1341 927 761
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BfrBI ATG'CAT
Cuts at: 287 1267 287
Size: 980 3438
BglI GCCn_nnn'nGGC
Cuts at: 679 679
Size: 4418
BglII A'GATC_T
Cuts at: 503 1933 3970 503
Size: 1430 2037 951
Fragments arranged by size:
2037 1430 951
BlpI GC'TnA_GC
Cuts at: 997 1530 997
Size: 533 3885
Bme1580I G_kGCm'C
Cuts at: 2043 2136 3192 2043
Size: 93 1056 3269
Fragments arranged by size:
3269 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 600 700 1630 1711 2046 4190 600
Size: 100 930 81 335 2144 828
Fragments arranged by size:
2144 930 828 335 100 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 562 706 124
Size: 438 144 3836
Fragments arranged by size:
3836 438 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1594 2473 2965 3263 1594
Size: 879 492 298 2749
Fragments arranged by size:
2749 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4122 4122
Size: 4418
BsaAI yAC'GTr
Cuts at: 1771 1771
Size: 4418
BsaBI GATnn'nnATC
Cuts at: 6 1932 3969 6
Size: 1926 2037 455
Fragments arranged by size:
2037 1926 455
BsaHI Gr'CG_yC
Cuts at: 1849 2097 2799 4295 1849
Size: 248 702 1496 1972
Fragments arranged by size:
1972 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1607 2128 2806 3080 3227 3638 1607
Size: 521 678 274 147 411 2387
Fragments arranged by size:
2387 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 703
Size: 4418
BseYI C'CCAG_C
Cuts at: 1843 2601 3178 3674 1843
Size: 758 577 496 2587
Fragments arranged by size:
2587 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 496
Size: 4418
BsiEI CG_ry'CG
Cuts at: 104 1960 2006 3214 104
Size: 1856 46 1208 1308
Fragments arranged by size:
1856 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 695 1379 1432 2210 2400 3192 695
Size: 684 53 778 190 792 1921
Fragments arranged by size:
1921 792 778 684 190 53
BsmI GAATG_Cn'
Cuts at: 289 497 991 289
Size: 208 494 3716
Fragments arranged by size:
3716 494 208
Bsp1286I G_dGCh'C
Cuts at: 695 1379 1432 2043 2136 2210 2400 2462 3192
Size: 684 53 611 93 74 190 62 730
Cuts at: 3192 695
Size: 1921
Fragments arranged by size:
1921 730 684 611 190 93 74 62
53
BspEI T'CCGG_A
Cuts at: 3638 3638
Size: 4418
BspHI T'CATG_A
Cuts at: 3594 3594
Size: 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1468 1682 1984 2365 1468
Size: 214 302 381 3521
Fragments arranged by size:
3521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2710 2764 4248 4413 50
Size: 2660 54 1484 165 55
Fragments arranged by size:
2660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 434 2330 434
Size: 1896 2522
BsrFI r'CCGG_y
Cuts at: 2416 2597 2806 2416
Size: 181 209 4028
Fragments arranged by size:
4028 209 181
BsrGI T'GTAC_A
Cuts at: 347 347
Size: 4418
BssSI C'ACGA_G
Cuts at: 2689 3047 2689
Size: 358 4060
BstAPI GCAn_nnn'nTGC
Cuts at: 232 1795 232
Size: 1563 2855
BstBI TT'CG_AA
Cuts at: 2779 2779
Size: 4418
BstEII G'GTnAC_C
Cuts at: 595 595
Size: 4418
BstXI CCAn_nnnn'nTGG
Cuts at: 835 1258 835
Size: 423 3995
BstYI r'GATC_y
Cuts at: 1 503 895 1443 1910 1933 2268 2514 3526
Size: 502 392 548 467 23 335 246 1012
Cuts at: 3526 3612 3624 3970 1
Size: 86 12 346 449
Fragments arranged by size:
1012 548 502 467 449 392 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1214 1214
Size: 4418
Bsu36I CC'TnA_GG
Cuts at: 865 865
Size: 4418
BtgI C'CryG_G
Cuts at: 107 107
Size: 4418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2368 2549 2712 2368
Size: 181 163 4074
Fragments arranged by size:
4074 181 163
BtsI GCAGTG_nn'
Cuts at: 113 233 644 1661 113
Size: 120 411 1017 2870
Fragments arranged by size:
2870 1017 411 120
DraI TTT'AAA
Cuts at: 1417 1417
Size: 4418
DrdI GACnn_nn'nnGTC
Cuts at: 477 2124 2982 477
Size: 1647 858 1913
Fragments arranged by size:
1913 1647 858
EaeI y'GGCC_r
Cuts at: 2003 2177 2568 2595 3940 2003
Size: 174 391 27 1345 2481
Fragments arranged by size:
2481 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2003 2003
Size: 4418
EarI CTCTTCn'nnn_
Cuts at: 2441 2651 2441
Size: 210 4208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2626 2936 3082 4228 2626
Size: 310 146 1146 2816
Fragments arranged by size:
2816 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1377 1430 1377
Size: 53 4365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 562 706 2243 2675 3422 3690 124
Size: 438 144 1537 432 747 268 852
Fragments arranged by size:
1537 852 747 438 432 268 144
EcoNI CCTnn'n_nnAGG
Cuts at: 1408 1408
Size: 4418
EcoO109I rG'GnC_Cy
Cuts at: 662 681 787 978 1557 662
Size: 19 106 191 579 3523
Fragments arranged by size:
3523 579 191 106 19
EcoRI G'AATT_C
Cuts at: 1422 1422
Size: 4418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4418
FspI TGC'GCA
Cuts at: 2199 2199
Size: 4418
HaeII r_GCGC'y
Cuts at: 1852 2100 3122 4101 1852
Size: 248 1022 979 2169
Fragments arranged by size:
2169 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 363 395 530 562 572 604 651 683 724
Size: 32 135 32 10 32 47 32 41
Cuts at: 724 756 1288 1320 2260 2290 2292 2322 363
Size: 32 532 32 940 30 2 30 2459
Fragments arranged by size:
2459 940 532 135 47 41 32 32
32 32 32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 1457 1457
Size: 4418
KasI G'GCGC_C
Cuts at: 1848 2096 1848
Size: 248 4170
KpnI G_GTAC'C
Cuts at: 1055 1438 1055
Size: 383 4035
MfeI C'AATT_G
Cuts at: 194 1195 194
Size: 1001 3417
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 336 1181 3089 3273 336
Size: 845 1908 184 1481
Fragments arranged by size:
1908 1481 845 184
MscI TGG'CCA
Cuts at: 2179 2179
Size: 4418
MslI CAynn'nnrTG
Cuts at: 833 884 2407 2534 833
Size: 51 1523 127 2717
Fragments arranged by size:
2717 1523 127 51
MspA1I CmG'CkG
Cuts at: 147 1301 1529 1843 2203 3216 3461 147
Size: 1154 228 314 360 1013 245 1104
Fragments arranged by size:
1154 1104 1013 360 314 245 228
NaeI GCC'GGC
Cuts at: 2599 2599
Size: 4418
NarI GG'CG_CC
Cuts at: 1849 2097 1849
Size: 248 4170
NcoI C'CATG_G
Cuts at: 107 107
Size: 4418
NgoMIV G'CCGG_C
Cuts at: 2597 2597
Size: 4418
NsiI A_TGCA'T
Cuts at: 289 1269 289
Size: 980 3438
NspI r_CATG'y
Cuts at: 156 889 1212 1471 1971 2878 156
Size: 733 323 259 500 907 1696
Fragments arranged by size:
1696 907 733 500 323 259
PciI A'CATG_T
Cuts at: 885 1208 2874 885
Size: 323 1666 2429
Fragments arranged by size:
2429 1666 323
PflMI CCAn_nnn'nTGG
Cuts at: 684 1200 684
Size: 516 3902
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4386
PstI C_TGCA'G
Cuts at: 651 1465 2150 651
Size: 814 685 2919
Fragments arranged by size:
2919 814 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 147 1301 1843 2203 147
Size: 1154 542 360 2362
Fragments arranged by size:
2362 1154 542 360
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 1379 1432 1379
Size: 53 4365
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SapI GCTCTTCn'nnn_
Cuts at: 2441 2651 2441
Size: 210 4208
SbfI CC_TGCA'GG
Cuts at: 1465 1465
Size: 4418
ScaI AGT'ACT
Cuts at: 3935 3935
Size: 4418
SfcI C'TryA_G
Cuts at: 33 647 1461 2146 3139 3330 33
Size: 614 814 685 993 191 1121
Fragments arranged by size:
1121 993 814 685 614 191
SfoI GGC'GCC
Cuts at: 1850 2098 1850
Size: 248 4170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SmaI CCC'GGG
Cuts at: 1440 1440
Size: 4418
SmlI C'TyrA_G
Cuts at: 1573 2488 2980 3242 1573
Size: 915 492 262 2749
Fragments arranged by size:
2749 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 1471 1971 1471
Size: 500 3918
StyI C'CwwG_G
Cuts at: 107 942 1552 1906 107
Size: 835 610 354 2619
Fragments arranged by size:
2619 835 610 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 691 2001 2134 2593 2802 691
Size: 1310 133 459 209 2307
Fragments arranged by size:
2307 1310 459 209 133
TatI w'GTAC_w
Cuts at: 347 3933 347
Size: 3586 832
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4386
Tth111I GACn'n_nGTC
Cuts at: 2215 2215
Size: 4418
XbaI T'CTAG_A
Cuts at: 1449 1449
Size: 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 681 1913 681
Size: 1232 3186
XmaI C'CCGG_G
Cuts at: 1438 1438
Size: 4418
XmnI GAAnn'nnTTC
Cuts at: 401 401
Size: 4418
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BssHII ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI