Restriction map for
pF1KB8177
(Circular) (Six-base) MAPSORT of: pF1KB8177.seq Check: 3313 from: 1 to: 4715
pF1KB8177 4715 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 642 642
Size: 4715
AccI GT'mk_AC
Cuts at: 1485 1753 1485
Size: 268 4447
Acc65I G'GTAC_C
Cuts at: 594 1731 594
Size: 1137 3578
AclI AA'CG_TT
Cuts at: 4555 4555
Size: 4715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 972 2540 2972 3719 3987 554
Size: 418 1568 432 747 268 1282
Fragments arranged by size:
1568 1282 747 432 418 268
AflIII A'CryG_T
Cuts at: 665 2067 3171 4148 665
Size: 1402 1104 977 1232
Fragments arranged by size:
1402 1232 1104 977
AgeI A'CCGG_T
Cuts at: 3103 3103
Size: 4715
AleI CACnn'nnGTG
Cuts at: 314 550 845 314
Size: 236 295 4184
Fragments arranged by size:
4184 295 236
AlwNI CAG_nnn'CTG
Cuts at: 619 1588 2020 3587 619
Size: 969 432 1567 1747
Fragments arranged by size:
1747 1567 969 432
ApaLI G'TGCA_C
Cuts at: 307 1692 3485 307
Size: 1385 1793 1537
Fragments arranged by size:
1793 1537 1385
ApoI r'AATT_y
Cuts at: 609 1311 1331 1719 4068 4324 609
Size: 702 20 388 2349 256 1000
Fragments arranged by size:
2349 1000 702 388 256 20
AseI AT'TA_AT
Cuts at: 20 653 20
Size: 633 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
AvaI C'yCGr_G
Cuts at: 1735 1735
Size: 4715
BamHI G'GATC_C
Cuts at: 1740 1740
Size: 4715
BanI G'GyrC_C
Cuts at: 594 1731 2145 2393 2428 594
Size: 1137 414 248 35 2881
Fragments arranged by size:
2881 1137 414 248 35
BanII G_rGCy'C
Cuts at: 746 788 1729 2759 746
Size: 42 941 1030 2702
Fragments arranged by size:
2702 1030 941 42
BbeI G_GCGC'C
Cuts at: 2149 2397 2149
Size: 248 4467
BbsI GAAGACnn'nnnn_
Cuts at: 965 1256 965
Size: 291 4424
BciVI GTATCCnnnnn_n'
Cuts at: 2613 3374 4715 2613
Size: 761 1341 2613
Fragments arranged by size:
2613 1341 761
BclI T'GATC_A
Cuts at: 1032 1577 2235 1032
Size: 545 658 3512
Fragments arranged by size:
3512 658 545
BglII A'GATC_T
Cuts at: 983 2230 4267 983
Size: 1247 2037 1431
Fragments arranged by size:
2037 1431 1247
BlpI GC'TnA_GC
Cuts at: 1827 1827
Size: 4715
Bme1580I G_kGCm'C
Cuts at: 311 1696 2340 2433 3489 311
Size: 1385 644 93 1056 1537
Fragments arranged by size:
1537 1385 1056 644 93
BmrI ACTGGGnnnn_n'
Cuts at: 333 1927 2008 2343 4487 333
Size: 1594 81 335 2144 561
Fragments arranged by size:
2144 1594 561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 4715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1177 1309 1696 434
Size: 743 132 387 3453
Fragments arranged by size:
3453 743 387 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 1891 2770 3262 3560 98
Size: 1793 879 492 298 1253
Fragments arranged by size:
1793 1253 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 493 750 1190 1600 4419 493
Size: 257 440 410 2819 789
Fragments arranged by size:
2819 789 440 410 257
BsaAI yAC'GTr
Cuts at: 2068 2068
Size: 4715
BsaBI GATnn'nnATC
Cuts at: 6 1037 2229 4266 6
Size: 1031 1192 2037 455
Fragments arranged by size:
2037 1192 1031 455
BsaHI Gr'CG_yC
Cuts at: 2146 2394 3096 4592 2146
Size: 248 702 1496 2269
Fragments arranged by size:
2269 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1904 2425 3103 3377 3524 3935 1904
Size: 521 678 274 147 411 2684
Fragments arranged by size:
2684 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 152 182 424 454 152
Size: 30 242 30 4413
Fragments arranged by size:
4413 242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 726 1585 445
Size: 281 859 3575
Fragments arranged by size:
3575 859 281
BseYI C'CCAG_C
Cuts at: 736 962 2140 2898 3475 3971 736
Size: 226 1178 758 577 496 1480
Fragments arranged by size:
1480 1178 758 577 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 1562 363
Size: 1199 3516
BsiEI CG_ry'CG
Cuts at: 104 2257 2303 3511 104
Size: 2153 46 1208 1308
Fragments arranged by size:
2153 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 311 954 1696 1729 2507 2697 3489 311
Size: 643 742 33 778 190 792 1537
Fragments arranged by size:
1537 792 778 742 643 190 33
BsmI GAATG_Cn'
Cuts at: 365 365
Size: 4715
Bsp1286I G_dGCh'C
Cuts at: 311 746 788 954 1696 1729 2340 2433 2507
Size: 435 42 166 742 33 611 93 74
Cuts at: 2507 2697 2759 3489 311
Size: 190 62 730 1537
Fragments arranged by size:
1537 742 730 611 435 190 166 93
74 62 42 33
BspEI T'CCGG_A
Cuts at: 3935 3935
Size: 4715
BspHI T'CATG_A
Cuts at: 3891 3891
Size: 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 642 1765 1979 2281 2662 642
Size: 1123 214 302 381 2695
Fragments arranged by size:
2695 1123 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3007 3061 4545 4710 50
Size: 2957 54 1484 165 55
Fragments arranged by size:
2957 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1265 1517 2627 1265
Size: 252 1110 3353
Fragments arranged by size:
3353 1110 252
BsrFI r'CCGG_y
Cuts at: 199 488 2713 2894 3103 199
Size: 289 2225 181 209 1811
Fragments arranged by size:
2225 1811 289 209 181
BsrGI T'GTAC_A
Cuts at: 401 576 401
Size: 175 4540
BssSI C'ACGA_G
Cuts at: 2986 3344 2986
Size: 358 4357
BstAPI GCAn_nnn'nTGC
Cuts at: 2092 2092
Size: 4715
BstBI TT'CG_AA
Cuts at: 3076 3076
Size: 4715
BstYI r'GATC_y
Cuts at: 1 983 991 1646 1740 2207 2230 2565 2811
Size: 982 8 655 94 467 23 335 246
Cuts at: 2811 3823 3909 3921 4267 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 982 655 467 449 346 335 246
94 86 23 12 8
BtgI C'CryG_G
Cuts at: 503 521 503
Size: 18 4697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2665 2846 3009 2665
Size: 181 163 4371
Fragments arranged by size:
4371 181 163
BtsI GCAGTG_nn'
Cuts at: 1958 1958
Size: 4715
DraI TTT'AAA
Cuts at: 1714 1714
Size: 4715
DrdI GACnn_nn'nnGTC
Cuts at: 2421 3279 2421
Size: 858 3857
EaeI y'GGCC_r
Cuts at: 2300 2474 2865 2892 4237 2300
Size: 174 391 27 1345 2778
Fragments arranged by size:
2778 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2300 2300
Size: 4715
EarI CTCTTCn'nnn_
Cuts at: 1696 2738 2948 1696
Size: 1042 210 3463
Fragments arranged by size:
3463 1042 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2923 3233 3379 4525 2923
Size: 310 146 1146 3113
Fragments arranged by size:
3113 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1727 1727
Size: 4715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 554 972 1177 1309 1696 2540 2972 3719
Size: 120 418 205 132 387 844 432 747
Cuts at: 3719 3987 434
Size: 268 1162
Fragments arranged by size:
1162 844 747 432 418 387 268 205
132 120
EcoNI CCTnn'n_nnAGG
Cuts at: 1123 1123
Size: 4715
EcoO109I rG'GnC_Cy
Cuts at: 1364 1854 1364
Size: 490 4225
EcoRI G'AATT_C
Cuts at: 609 1719 609
Size: 1110 3605
EcoRV GAT'ATC
Cuts at: 370 370
Size: 4715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4715
FspI TGC'GCA
Cuts at: 2496 2496
Size: 4715
HaeII r_GCGC'y
Cuts at: 2149 2397 3419 4398 2149
Size: 248 1022 979 2466
Fragments arranged by size:
2466 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1569 1601 2557 2587 2589 2619 1569
Size: 32 956 30 2 30 3665
Fragments arranged by size:
3665 956 32 30 30 2
HincII GTy'rAC
Cuts at: 1626 1754 1626
Size: 128 4587
KasI G'GCGC_C
Cuts at: 2145 2393 2145
Size: 248 4467
KpnI G_GTAC'C
Cuts at: 598 1735 598
Size: 1137 3578
MluI A'CGCG_T
Cuts at: 4148 4148
Size: 4715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 306 1056 1613 3386 3570 306
Size: 750 557 1773 184 1451
Fragments arranged by size:
1773 1451 750 557 184
MscI TGG'CCA
Cuts at: 2476 2476
Size: 4715
MslI CAynn'nnrTG
Cuts at: 314 550 845 2704 2831 314
Size: 236 295 1859 127 2198
Fragments arranged by size:
2198 1859 295 236 127
MspA1I CmG'CkG
Cuts at: 466 1399 1826 2140 2500 3513 3758 466
Size: 933 427 314 360 1013 245 1423
Fragments arranged by size:
1423 1013 933 427 360 314 245
NaeI GCC'GGC
Cuts at: 2896 2896
Size: 4715
NarI GG'CG_CC
Cuts at: 2146 2394 2146
Size: 248 4467
NcoI C'CATG_G
Cuts at: 503 521 503
Size: 18 4697
NgoMIV G'CCGG_C
Cuts at: 2894 2894
Size: 4715
NspI r_CATG'y
Cuts at: 669 1768 2268 3175 669
Size: 1099 500 907 2209
Fragments arranged by size:
2209 1099 907 500
PasI CC'CwG_GG
Cuts at: 619 619
Size: 4715
PciI A'CATG_T
Cuts at: 665 3171 665
Size: 2506 2209
PflMI CCAn_nnn'nTGG
Cuts at: 799 799
Size: 4715
PfoI T'CCnGG_A
Cuts at: 1325 1360 1325
Size: 35 4680
PmeI GTTT'AAAC
Cuts at: 1714 1714
Size: 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 4683
PpuMI rG'GwC_Cy
Cuts at: 1364 1364
Size: 4715
PstI C_TGCA'G
Cuts at: 1762 2447 1762
Size: 685 4030
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4715
PvuII CAG'CTG
Cuts at: 466 1399 2140 2500 466
Size: 933 741 360 2681
Fragments arranged by size:
2681 933 741 360
RsrII CG'GwC_CG
Cuts at: 2910 2910
Size: 4715
SacI G_AGCT'C
Cuts at: 1729 1729
Size: 4715
SalI G'TCGA_C
Cuts at: 1752 1752
Size: 4715
SapI GCTCTTCn'nnn_
Cuts at: 2738 2948 2738
Size: 210 4505
SbfI CC_TGCA'GG
Cuts at: 1762 1762
Size: 4715
ScaI AGT'ACT
Cuts at: 4232 4232
Size: 4715
SexAI A'CCwGG_T
Cuts at: 290 671 290
Size: 381 4334
SfcI C'TryA_G
Cuts at: 33 770 1758 2443 3436 3627 33
Size: 737 988 685 993 191 1121
Fragments arranged by size:
1121 993 988 737 685 191
SfoI GGC'GCC
Cuts at: 2147 2395 2147
Size: 248 4467
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
SmaI CCC'GGG
Cuts at: 1737 1737
Size: 4715
SmlI C'TyrA_G
Cuts at: 113 1870 2785 3277 3539 113
Size: 1757 915 492 262 1289
Fragments arranged by size:
1757 1289 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4715
SphI G_CATG'C
Cuts at: 1768 2268 1768
Size: 500 4215
SspI AAT'ATT
Cuts at: 1202 1202
Size: 4715
StyI C'CwwG_G
Cuts at: 503 521 746 1849 2203 503
Size: 18 225 1103 354 3015
Fragments arranged by size:
3015 1103 354 225 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 976 2298 2431 2890 3099 976
Size: 1322 133 459 209 2592
Fragments arranged by size:
2592 1322 459 209 133
TatI w'GTAC_w
Cuts at: 401 576 4230 401
Size: 175 3654 886
Fragments arranged by size:
3654 886 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 4683
Tth111I GACn'n_nGTC
Cuts at: 459 2512 459
Size: 2053 2662
XbaI T'CTAG_A
Cuts at: 1746 1746
Size: 4715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1152 1671 2210 1152
Size: 519 539 3657
Fragments arranged by size:
3657 539 519
XmaI C'CCGG_G
Cuts at: 1735 1735
Size: 4715
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI