Restriction map for
pF1KB8170
(Circular) (Six-base) MAPSORT of: pF1KB8170.seq Check: 7221 from: 1 to: 4220
pF1KB8170 4220 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1258 1258
Size: 4220
Acc65I G'GTAC_C
Cuts at: 1236 1236
Size: 4220
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 379 469 560 658 850 1008 1186 2045
Size: 192 90 91 98 192 158 178 859
Cuts at: 2045 2477 3224 3492 187
Size: 432 747 268 915
Fragments arranged by size:
915 859 747 432 268 192 192 178
158 98 91 90
AfeI AGC'GCT
Cuts at: 703 703
Size: 4220
AflIII A'CryG_T
Cuts at: 959 1572 2676 3653 959
Size: 613 1104 977 1526
Fragments arranged by size:
1526 1104 977 613
AgeI A'CCGG_T
Cuts at: 2608 2608
Size: 4220
AhdI GACnn_n'nnGTC
Cuts at: 150 150
Size: 4220
AleI CACnn'nnGTG
Cuts at: 190 190
Size: 4220
AlwNI CAG_nnn'CTG
Cuts at: 423 569 757 909 982 1525 3092 423
Size: 146 188 152 73 543 1567 1551
Fragments arranged by size:
1567 1551 543 188 152 146 73
ApaI G_GGCC'C
Cuts at: 454 711 747 454
Size: 257 36 3927
Fragments arranged by size:
3927 257 36
ApaLI G'TGCA_C
Cuts at: 2990 2990
Size: 4220
ApoI r'AATT_y
Cuts at: 508 1224 3573 3829 508
Size: 716 2349 256 899
Fragments arranged by size:
2349 899 716 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 746 1240 746
Size: 494 3726
BamHI G'GATC_C
Cuts at: 539 1245 539
Size: 706 3514
BanI G'GyrC_C
Cuts at: 284 880 1236 1650 1898 1933 284
Size: 596 356 414 248 35 2571
Fragments arranged by size:
2571 596 414 356 248 35
BanII G_rGCy'C
Cuts at: 390 454 587 711 747 1234 2264 390
Size: 64 133 124 36 487 1030 2346
Fragments arranged by size:
2346 1030 487 133 124 64 36
BbeI G_GCGC'C
Cuts at: 288 1654 1902 288
Size: 1366 248 2606
Fragments arranged by size:
2606 1366 248
BbsI GAAGACnn'nnnn_
Cuts at: 263 263
Size: 4220
BbvCI CC'TCA_GC
Cuts at: 1210 1210
Size: 4220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 207 241 207
Size: 34 4186
BciVI GTATCCnnnnn_n'
Cuts at: 2118 2879 4220 2118
Size: 761 1341 2118
Fragments arranged by size:
2118 1341 761
BclI T'GATC_A
Cuts at: 1740 1740
Size: 4220
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 659 1139 1160 1332 659
Size: 480 21 172 3547
Fragments arranged by size:
3547 480 172 21
Bme1580I G_kGCm'C
Cuts at: 454 711 747 885 1182 1845 1938 2994 454
Size: 257 36 138 297 663 93 1056 1680
Fragments arranged by size:
1680 1056 663 297 257 138 93 36
BmgBI CAC'GTC
Cuts at: 531 531
Size: 4220
BmrI ACTGGGnnnn_n'
Cuts at: 1432 1513 1848 3992 1432
Size: 81 335 2144 1660
Fragments arranged by size:
2144 1660 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 277 745 1069 271
Size: 6 468 324 3422
Fragments arranged by size:
3422 468 324 6
Bpu10I CC'TnA_GC
Cuts at: 1210 1210
Size: 4220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 646 1396 2275 2767 3065 319
Size: 327 750 879 492 298 1474
Fragments arranged by size:
1474 879 750 492 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 253 365 3924 253
Size: 112 3559 549
Fragments arranged by size:
3559 549 112
BsaAI yAC'GTr
Cuts at: 1573 1573
Size: 4220
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 285 1651 1899 2601 4097 285
Size: 1366 248 702 1496 408
Fragments arranged by size:
1496 1366 702 408 248
BsaWI w'CCGG_w
Cuts at: 1409 1930 2608 2882 3029 3440 1409
Size: 521 678 274 147 411 2189
Fragments arranged by size:
2189 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 625 643 742 1081 244
Size: 381 18 99 339 3383
Fragments arranged by size:
3383 381 339 99 18
BseYI C'CCAG_C
Cuts at: 363 698 704 845 1645 2403 2980 3476 363
Size: 335 6 141 800 758 577 496 1107
Fragments arranged by size:
1107 800 758 577 496 335 141 6
BsiEI CG_ry'CG
Cuts at: 104 1762 1808 3016 104
Size: 1658 46 1208 1308
Fragments arranged by size:
1658 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 390 1234 2012 2202 2994 390
Size: 844 778 190 792 1616
Fragments arranged by size:
1616 844 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 390 454 587 711 747 885 1182 1234 1845
Size: 64 133 124 36 138 297 52 611
Cuts at: 1845 1938 2012 2202 2264 2994 390
Size: 93 74 190 62 730 1616
Fragments arranged by size:
1616 730 611 297 190 138 133 124
93 74 64 62 52 36
BspEI T'CCGG_A
Cuts at: 3440 3440
Size: 4220
BspHI T'CATG_A
Cuts at: 3396 3396
Size: 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 728 1214 1270 1484 1786 2167 728
Size: 486 56 214 302 381 2781
Fragments arranged by size:
2781 486 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 2512 2566 4050 4215 50
Size: 2462 54 1484 165 55
Fragments arranged by size:
2462 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2132 2132
Size: 4220
BsrFI r'CCGG_y
Cuts at: 281 2218 2399 2608 281
Size: 1937 181 209 1893
Fragments arranged by size:
1937 1893 209 181
BssSI C'ACGA_G
Cuts at: 2491 2849 2491
Size: 358 3862
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1206 1597 1177
Size: 29 391 3800
Fragments arranged by size:
3800 391 29
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstXI CCAn_nnnn'nTGG
Cuts at: 382 382
Size: 4220
BstYI r'GATC_y
Cuts at: 1 314 494 539 671 1245 1712 1735 2070
Size: 313 180 45 132 574 467 23 335
Cuts at: 2070 2316 3328 3414 3426 3772 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 574 467 449 346 335 313 246
180 132 86 45 23 12
BtgI C'CryG_G
Cuts at: 535 535
Size: 4220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2170 2351 2514 2170
Size: 181 163 3876
Fragments arranged by size:
3876 181 163
BtsI GCAGTG_nn'
Cuts at: 197 1463 197
Size: 1266 2954
DraI TTT'AAA
Cuts at: 1219 1219
Size: 4220
DraIII CAC_nnn'GTG
Cuts at: 1177 1177
Size: 4220
DrdI GACnn_nn'nnGTC
Cuts at: 267 1926 2784 267
Size: 1659 858 1703
Fragments arranged by size:
1703 1659 858
EaeI y'GGCC_r
Cuts at: 1805 1979 2370 2397 3742 1805
Size: 174 391 27 1345 2283
Fragments arranged by size:
2283 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2428 2738 2884 4030 2428
Size: 310 146 1146 2618
Fragments arranged by size:
2618 1146 310 146
EcoICRI GAG'CTC
Cuts at: 388 1232 388
Size: 844 3376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 271 277 379 469 560 658 745 850
Size: 84 6 102 90 91 98 87 105
Cuts at: 850 1008 1069 1186 2045 2477 3224 3492 187
Size: 158 61 117 859 432 747 268 915
Fragments arranged by size:
915 859 747 432 268 158 117 105
102 98 91 90 87 84 61 6
EcoNI CCTnn'n_nnAGG
Cuts at: 458 724 901 913 458
Size: 266 177 12 3765
Fragments arranged by size:
3765 266 177 12
EcoO109I rG'GnC_Cy
Cuts at: 451 717 743 906 1083 1131 1359 451
Size: 266 26 163 177 48 228 3312
Fragments arranged by size:
3312 266 228 177 163 48 26
EcoRI G'AATT_C
Cuts at: 508 1224 508
Size: 716 3504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 4220
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 288 705 897 1654 1902 2924 3903 288
Size: 417 192 757 248 1022 979 605
Fragments arranged by size:
1022 979 757 605 417 248 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 2062 2092 2094 2124 136
Size: 32 1894 30 2 30 2232
Fragments arranged by size:
2232 1894 32 30 30 2
HincII GTy'rAC
Cuts at: 1259 1259
Size: 4220
KasI G'GCGC_C
Cuts at: 284 1650 1898 284
Size: 1366 248 2606
Fragments arranged by size:
2606 1366 248
KpnI G_GTAC'C
Cuts at: 1240 1240
Size: 4220
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2891 3075 2891
Size: 184 4036
MscI TGG'CCA
Cuts at: 1981 1981
Size: 4220
MslI CAynn'nnrTG
Cuts at: 190 2209 2336 190
Size: 2019 127 2074
Fragments arranged by size:
2074 2019 127
MspA1I CmG'CkG
Cuts at: 249 754 1331 1645 2005 3018 3263 249
Size: 505 577 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 577 505 360 314 245
NaeI GCC'GGC
Cuts at: 283 2401 283
Size: 2118 2102
NarI GG'CG_CC
Cuts at: 285 1651 1899 285
Size: 1366 248 2606
Fragments arranged by size:
2606 1366 248
NgoMIV G'CCGG_C
Cuts at: 281 2399 281
Size: 2118 2102
NspI r_CATG'y
Cuts at: 963 1273 1773 2680 963
Size: 310 500 907 2503
Fragments arranged by size:
2503 907 500 310
PasI CC'CwG_GG
Cuts at: 681 1080 681
Size: 399 3821
PciI A'CATG_T
Cuts at: 959 2676 959
Size: 1717 2503
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 4220
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 4188
PpuMI rG'GwC_Cy
Cuts at: 717 1131 717
Size: 414 3806
PspOMI G'GGCC_C
Cuts at: 450 707 743 450
Size: 257 36 3927
Fragments arranged by size:
3927 257 36
PstI C_TGCA'G
Cuts at: 248 905 1267 1952 248
Size: 657 362 685 2516
Fragments arranged by size:
2516 685 657 362
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 249 1645 2005 249
Size: 1396 360 2464
Fragments arranged by size:
2464 1396 360
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 390 1234 390
Size: 844 3376
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
SbfI CC_TGCA'GG
Cuts at: 905 1267 905
Size: 362 3858
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 244 901 1263 1948 2941 3132 33
Size: 211 657 362 685 993 191 1121
Fragments arranged by size:
1121 993 685 657 362 211 191
SfoI GGC'GCC
Cuts at: 286 1652 1900 286
Size: 1366 248 2606
Fragments arranged by size:
2606 1366 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 748 1242 748
Size: 494 3726
SmlI C'TyrA_G
Cuts at: 298 625 1375 2290 2782 3044 298
Size: 327 750 915 492 262 1474
Fragments arranged by size:
1474 915 750 492 327 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1273 1773 1273
Size: 500 3720
SrfI GCCC'GGGC
Cuts at: 748 748
Size: 4220
SspI AAT'ATT
Cuts at: 924 924
Size: 4220
StuI AGG'CCT
Cuts at: 779 779
Size: 4220
StyI C'CwwG_G
Cuts at: 1354 1708 1354
Size: 354 3866
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 811 1803 1936 2395 2604 811
Size: 992 133 459 209 2427
Fragments arranged by size:
2427 992 459 209 133
TatI w'GTAC_w
Cuts at: 3735 3735
Size: 4220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4188
Tth111I GACn'n_nGTC
Cuts at: 2017 2017
Size: 4220
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 1715
Size: 4220
XmaI C'CCGG_G
Cuts at: 746 1240 746
Size: 494 3726
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI AscI AvrII BaeI
BfrBI BglI BmtI BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SwaI XhoI XmnI ZraI