Restriction map for
pF1KB8166
(Circular) (Six-base) MAPSORT of: pF1KB8166.seq Check: 3836 from: 1 to: 5045
pF1KB8166 5045 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1783 1783
Size: 5045
AccI GT'mk_AC
Cuts at: 1220 1895 2083 1220
Size: 675 188 4182
Fragments arranged by size:
4182 675 188
Acc65I G'GTAC_C
Cuts at: 698 2061 698
Size: 1363 3682
AclI AA'CG_TT
Cuts at: 4885 4885
Size: 5045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 641 1481 2870 3302 4049 4317 196
Size: 445 840 1389 432 747 268 924
Fragments arranged by size:
1389 924 840 747 445 432 268
AflII C'TTAA_G
Cuts at: 1306 1306
Size: 5045
AflIII A'CryG_T
Cuts at: 2397 3501 4478 2397
Size: 1104 977 2964
Fragments arranged by size:
2964 1104 977
AgeI A'CCGG_T
Cuts at: 3433 3433
Size: 5045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1791 2002 1791
Size: 211 4834
AlwNI CAG_nnn'CTG
Cuts at: 1386 1621 1957 2350 3917 1386
Size: 235 336 393 1567 2514
Fragments arranged by size:
2514 1567 393 336 235
ApaLI G'TGCA_C
Cuts at: 3815 3815
Size: 5045
ApoI r'AATT_y
Cuts at: 409 451 710 2049 4398 4654 409
Size: 42 259 1339 2349 256 800
Fragments arranged by size:
2349 1339 800 259 256 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 5045
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5045
AvaI C'yCGr_G
Cuts at: 1526 2065 1526
Size: 539 4506
BamHI G'GATC_C
Cuts at: 495 2070 495
Size: 1575 3470
BanI G'GyrC_C
Cuts at: 698 1881 2061 2475 2723 2758 698
Size: 1183 180 414 248 35 2985
Fragments arranged by size:
2985 1183 414 248 180 35
BanII G_rGCy'C
Cuts at: 999 1252 2059 3089 999
Size: 253 807 1030 2955
Fragments arranged by size:
2955 1030 807 253
BbeI G_GCGC'C
Cuts at: 2479 2727 2479
Size: 248 4797
BbsI GAAGACnn'nnnn_
Cuts at: 146 443 1307 1646 146
Size: 297 864 339 3545
Fragments arranged by size:
3545 864 339 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 719 753 719
Size: 34 5011
BciVI GTATCCnnnnn_n'
Cuts at: 952 1830 2943 3704 5045 952
Size: 878 1113 761 1341 952
Fragments arranged by size:
1341 1113 952 878 761
BclI T'GATC_A
Cuts at: 2565 2565
Size: 5045
BglI GCCn_nnn'nGGC
Cuts at: 948 948
Size: 5045
BglII A'GATC_T
Cuts at: 1034 1356 2560 4597 1034
Size: 322 1204 2037 1482
Fragments arranged by size:
2037 1482 1204 322
BlpI GC'TnA_GC
Cuts at: 2157 2157
Size: 5045
Bme1580I G_kGCm'C
Cuts at: 2670 2763 3819 2670
Size: 93 1056 3896
Fragments arranged by size:
3896 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 981 2257 2338 2673 4817 981
Size: 1276 81 335 2144 1209
Fragments arranged by size:
2144 1276 1209 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 5045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 590
Size: 5045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 2221 3100 3592 3890 1717
Size: 504 879 492 298 2872
Fragments arranged by size:
2872 879 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 815 4749 815
Size: 3934 1111
BsaAI yAC'GTr
Cuts at: 2398 2398
Size: 5045
BsaBI GATnn'nnATC
Cuts at: 6 2559 4596 6
Size: 2553 2037 455
Fragments arranged by size:
2553 2037 455
BsaHI Gr'CG_yC
Cuts at: 2476 2724 3426 4922 2476
Size: 248 702 1496 2599
Fragments arranged by size:
2599 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2234 2755 3433 3707 3854 4265 2234
Size: 521 678 274 147 411 3014
Fragments arranged by size:
3014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1859 1889 1859
Size: 30 5015
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 962 1447 962
Size: 485 4560
BseYI C'CCAG_C
Cuts at: 2470 3228 3805 4301 2470
Size: 758 577 496 3214
Fragments arranged by size:
3214 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 2008 539
Size: 1469 3576
BsiEI CG_ry'CG
Cuts at: 104 2587 2633 3841 104
Size: 2483 46 1208 1308
Fragments arranged by size:
2483 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 735 2059 2837 3027 3819 735
Size: 1324 778 190 792 1961
Fragments arranged by size:
1961 1324 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 735 999 1252 2059 2670 2763 2837 3027 3089
Size: 264 253 807 611 93 74 190 62
Cuts at: 3089 3819 735
Size: 730 1961
Fragments arranged by size:
1961 807 730 611 264 253 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4265 4265
Size: 5045
BspHI T'CATG_A
Cuts at: 4221 4221
Size: 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 842 1783 1965 2095 2309 2611 2992 842
Size: 941 182 130 214 302 381 2895
Fragments arranged by size:
2895 941 381 302 214 182 130
BsrBI CCG'CTC
Cuts at: 50 3337 3391 4875 5040 50
Size: 3287 54 1484 165 55
Fragments arranged by size:
3287 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2957 2957
Size: 5045
BsrFI r'CCGG_y
Cuts at: 947 3043 3224 3433 947
Size: 2096 181 209 2559
Fragments arranged by size:
2559 2096 209 181
BssSI C'ACGA_G
Cuts at: 3316 3674 3316
Size: 358 4687
BstAPI GCAn_nnn'nTGC
Cuts at: 1957 2422 1957
Size: 465 4580
BstBI TT'CG_AA
Cuts at: 3406 3406
Size: 5045
BstEII G'GTnAC_C
Cuts at: 584 584
Size: 5045
BstXI CCAn_nnnn'nTGG
Cuts at: 789 789
Size: 5045
BstYI r'GATC_y
Cuts at: 1 495 1034 1356 2070 2537 2560 2895 3141
Size: 494 539 322 714 467 23 335 246
Cuts at: 3141 4153 4239 4251 4597 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 714 539 494 467 449 346 335
322 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 531 107
Size: 424 4621
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2995 3176 3339 2995
Size: 181 163 4701
Fragments arranged by size:
4701 181 163
BtsI GCAGTG_nn'
Cuts at: 735 770 2288 735
Size: 35 1518 3492
Fragments arranged by size:
3492 1518 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 599 634 599
Size: 35 5010
DraI TTT'AAA
Cuts at: 2044 2044
Size: 5045
DrdI GACnn_nn'nnGTC
Cuts at: 2751 3609 2751
Size: 858 4187
EaeI y'GGCC_r
Cuts at: 1225 2630 2804 3195 3222 4567 1225
Size: 1405 174 391 27 1345 1703
Fragments arranged by size:
1703 1405 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2630 2630
Size: 5045
EarI CTCTTCn'nnn_
Cuts at: 1694 3068 3278 1694
Size: 1374 210 3461
Fragments arranged by size:
3461 1374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 966 3253 3563 3709 4855 966
Size: 2287 310 146 1146 1156
Fragments arranged by size:
2287 1156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2057 2057
Size: 5045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 590 641 1481 2870 3302 4049 4317 196
Size: 394 51 840 1389 432 747 268 924
Fragments arranged by size:
1389 924 840 747 432 394 268 51
EcoNI CCTnn'n_nnAGG
Cuts at: 308 308
Size: 5045
EcoO109I rG'GnC_Cy
Cuts at: 1954 2184 1954
Size: 230 4815
EcoRI G'AATT_C
Cuts at: 451 2049 451
Size: 1598 3447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 226 879 1738 3064 226
Size: 653 859 1326 2207
Fragments arranged by size:
2207 1326 859 653
FspI TGC'GCA
Cuts at: 2826 2826
Size: 5045
HaeII r_GCGC'y
Cuts at: 780 2479 2727 3749 4728 780
Size: 1699 248 1022 979 1097
Fragments arranged by size:
1699 1097 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1019 1051 2887 2917 2919 2949 1019
Size: 32 1836 30 2 30 3115
Fragments arranged by size:
3115 1836 32 30 30 2
HincII GTy'rAC
Cuts at: 2084 2084
Size: 5045
HindIII A'AGCT_T
Cuts at: 124 1481 124
Size: 1357 3688
KasI G'GCGC_C
Cuts at: 2475 2723 2475
Size: 248 4797
KpnI G_GTAC'C
Cuts at: 702 2065 702
Size: 1363 3682
MfeI C'AATT_G
Cuts at: 919 919
Size: 5045
MluI A'CGCG_T
Cuts at: 4478 4478
Size: 5045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3716 3900 3716
Size: 184 4861
MscI TGG'CCA
Cuts at: 1227 2806 1227
Size: 1579 3466
MslI CAynn'nnrTG
Cuts at: 1984 3034 3161 1984
Size: 1050 127 3868
Fragments arranged by size:
3868 1050 127
MspA1I CmG'CkG
Cuts at: 751 1374 1383 1992 2156 2470 2830 3843 4088
Size: 623 9 609 164 314 360 1013 245
Cuts at: 4088 751
Size: 1708
Fragments arranged by size:
1708 1013 623 609 360 314 245 164
9
NaeI GCC'GGC
Cuts at: 3226 3226
Size: 5045
NarI GG'CG_CC
Cuts at: 2476 2724 2476
Size: 248 4797
NcoI C'CATG_G
Cuts at: 107 531 107
Size: 424 4621
NdeI CA'TA_TG
Cuts at: 329 329
Size: 5045
NgoMIV G'CCGG_C
Cuts at: 3224 3224
Size: 5045
NspI r_CATG'y
Cuts at: 2098 2598 3505 2098
Size: 500 907 3638
Fragments arranged by size:
3638 907 500
PciI A'CATG_T
Cuts at: 3501 3501
Size: 5045
PflMI CCAn_nnn'nTGG
Cuts at: 1005 1005
Size: 5045
PmeI GTTT'AAAC
Cuts at: 2044 2044
Size: 5045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 5013
PpuMI rG'GwC_Cy
Cuts at: 1954 1954
Size: 5045
PshAI GACnn'nnGTC
Cuts at: 889 889
Size: 5045
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 145 177 145
Size: 32 5013
PstI C_TGCA'G
Cuts at: 798 1391 2092 2777 798
Size: 593 701 685 3066
Fragments arranged by size:
3066 701 685 593
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5045
PvuII CAG'CTG
Cuts at: 751 1374 1383 1992 2470 2830 751
Size: 623 9 609 478 360 2966
Fragments arranged by size:
2966 623 609 478 360 9
RsrII CG'GwC_CG
Cuts at: 3240 3240
Size: 5045
SacI G_AGCT'C
Cuts at: 2059 2059
Size: 5045
SalI G'TCGA_C
Cuts at: 2082 2082
Size: 5045
SapI GCTCTTCn'nnn_
Cuts at: 3068 3278 3068
Size: 210 4835
SbfI CC_TGCA'GG
Cuts at: 2092 2092
Size: 5045
ScaI AGT'ACT
Cuts at: 154 4562 154
Size: 4408 637
SexAI A'CCwGG_T
Cuts at: 1904 1904
Size: 5045
SfcI C'TryA_G
Cuts at: 33 794 1387 1929 2088 2773 3766 3957 33
Size: 761 593 542 159 685 993 191 1121
Fragments arranged by size:
1121 993 761 685 593 542 191 159
SfoI GGC'GCC
Cuts at: 2477 2725 2477
Size: 248 4797
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5045
SmaI CCC'GGG
Cuts at: 2067 2067
Size: 5045
SmlI C'TyrA_G
Cuts at: 1306 1526 1732 2200 3115 3607 3869 1306
Size: 220 206 468 915 492 262 2482
Fragments arranged by size:
2482 915 492 468 262 220 206
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5045
SphI G_CATG'C
Cuts at: 2098 2598 2098
Size: 500 4545
StuI AGG'CCT
Cuts at: 718 1605 718
Size: 887 4158
StyI C'CwwG_G
Cuts at: 107 531 1427 1600 2179 2533 107
Size: 424 896 173 579 354 2619
Fragments arranged by size:
2619 896 579 424 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 2628 2761 3220 3429 879
Size: 1749 133 459 209 2495
Fragments arranged by size:
2495 1749 459 209 133
TatI w'GTAC_w
Cuts at: 152 218 4560 152
Size: 66 4342 637
Fragments arranged by size:
4342 637 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 300 332 725 757 4562 4594 300
Size: 32 393 32 3805 32 751
Fragments arranged by size:
3805 751 393 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2842 2842
Size: 5045
XbaI T'CTAG_A
Cuts at: 2076 2076
Size: 5045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2540
Size: 5045
XhoI C'TCGA_G
Cuts at: 1526 1526
Size: 5045
XmaI C'CCGG_G
Cuts at: 2065 2065
Size: 5045
XmnI GAAnn'nnTTC
Cuts at: 710 1204 1483 710
Size: 494 279 4272
Fragments arranged by size:
4272 494 279
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PsiI PspOMI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI