Restriction map for
pF1KB8160
(Circular) (Six-base) MAPSORT of: pF1KB8160.seq Check: 8209 from: 1 to: 3893
pF1KB8160 3893 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 931 931
Size: 3893
Acc65I G'GTAC_C
Cuts at: 909 909
Size: 3893
AclI AA'CG_TT
Cuts at: 3733 3733
Size: 3893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1718 2150 2897 3165 1718
Size: 432 747 268 2446
Fragments arranged by size:
2446 747 432 268
AflIII A'CryG_T
Cuts at: 413 1245 2349 3326 413
Size: 832 1104 977 980
Fragments arranged by size:
1104 980 977 832
AgeI A'CCGG_T
Cuts at: 2281 2281
Size: 3893
AleI CACnn'nnGTG
Cuts at: 520 520
Size: 3893
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 277
Size: 32 3861
AlwNI CAG_nnn'CTG
Cuts at: 257 531 1198 2765 257
Size: 274 667 1567 1385
Fragments arranged by size:
1567 1385 667 274
ApaLI G'TGCA_C
Cuts at: 2663 2663
Size: 3893
ApoI r'AATT_y
Cuts at: 188 291 880 897 3246 3502 188
Size: 103 589 17 2349 256 579
Fragments arranged by size:
2349 589 579 256 103 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 3893
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
AvaI C'yCGr_G
Cuts at: 913 913
Size: 3893
BamHI G'GATC_C
Cuts at: 918 918
Size: 3893
BanI G'GyrC_C
Cuts at: 376 756 909 1323 1571 1606 376
Size: 380 153 414 248 35 2663
Fragments arranged by size:
2663 414 380 248 153 35
BanII G_rGCy'C
Cuts at: 155 251 907 1937 155
Size: 96 656 1030 2111
Fragments arranged by size:
2111 1030 656 96
BbeI G_GCGC'C
Cuts at: 1327 1575 1327
Size: 248 3645
BbsI GAAGACnn'nnnn_
Cuts at: 416 416
Size: 3893
BbvCI CC'TCA_GC
Cuts at: 817 817
Size: 3893
BciVI GTATCCnnnnn_n'
Cuts at: 1791 2552 3893 1791
Size: 761 1341 1791
Fragments arranged by size:
1791 1341 761
BclI T'GATC_A
Cuts at: 1413 1413
Size: 3893
BfrBI ATG'CAT
Cuts at: 752 752
Size: 3893
BglI GCCn_nnn'nGGC
Cuts at: 358 358
Size: 3893
BglII A'GATC_T
Cuts at: 1408 3445 1408
Size: 2037 1856
BlpI GC'TnA_GC
Cuts at: 250 1005 250
Size: 755 3138
Bme1580I G_kGCm'C
Cuts at: 581 773 869 1518 1611 2667 581
Size: 192 96 649 93 1056 1807
Fragments arranged by size:
1807 1056 649 192 96 93
BmrI ACTGGGnnnn_n'
Cuts at: 858 1105 1186 1521 3665 858
Size: 247 81 335 2144 1086
Fragments arranged by size:
2144 1086 335 247 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 3893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 169 608 105
Size: 64 439 3390
Fragments arranged by size:
3390 439 64
Bpu10I CC'TnA_GC
Cuts at: 817 817
Size: 3893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1948 2440 2738 1069
Size: 879 492 298 2224
Fragments arranged by size:
2224 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 3893
BsaAI yAC'GTr
Cuts at: 1246 1246
Size: 3893
BsaBI GATnn'nnATC
Cuts at: 6 1407 3444 6
Size: 1401 2037 455
Fragments arranged by size:
2037 1401 455
BsaHI Gr'CG_yC
Cuts at: 1324 1572 2274 3770 1324
Size: 248 702 1496 1447
Fragments arranged by size:
1496 1447 702 248
BsaWI w'CCGG_w
Cuts at: 1082 1603 2281 2555 2702 3113 1082
Size: 521 678 274 147 411 1862
Fragments arranged by size:
1862 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 440 440
Size: 3893
BseYI C'CCAG_C
Cuts at: 1318 2076 2653 3149 1318
Size: 758 577 496 2062
Fragments arranged by size:
2062 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 544 418
Size: 126 3767
BsiEI CG_ry'CG
Cuts at: 104 1435 1481 2689 104
Size: 1331 46 1208 1308
Fragments arranged by size:
1331 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 155 251 907 1685 1875 2667 155
Size: 96 656 778 190 792 1381
Fragments arranged by size:
1381 792 778 656 190 96
BsmI GAATG_Cn'
Cuts at: 403 754 403
Size: 351 3542
Bsp1286I G_dGCh'C
Cuts at: 155 251 581 773 869 907 1518 1611 1685
Size: 96 330 192 96 38 611 93 74
Cuts at: 1685 1875 1937 2667 155
Size: 190 62 730 1381
Fragments arranged by size:
1381 730 611 330 192 190 96 96
93 74 62 38
BspEI T'CCGG_A
Cuts at: 3113 3113
Size: 3893
BspHI T'CATG_A
Cuts at: 405 3069 405
Size: 2664 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 717 943 1157 1459 1840 717
Size: 226 214 302 381 2770
Fragments arranged by size:
2770 381 302 226 214
BsrBI CCG'CTC
Cuts at: 50 2185 2239 3723 3888 50
Size: 2135 54 1484 165 55
Fragments arranged by size:
2135 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 569 581 1805 569
Size: 12 1224 2657
Fragments arranged by size:
2657 1224 12
BsrFI r'CCGG_y
Cuts at: 759 1891 2072 2281 759
Size: 1132 181 209 2371
Fragments arranged by size:
2371 1132 209 181
BsrGI T'GTAC_A
Cuts at: 857 857
Size: 3893
BssSI C'ACGA_G
Cuts at: 772 2164 2522 772
Size: 1392 358 2143
Fragments arranged by size:
2143 1392 358
BstAPI GCAn_nnn'nTGC
Cuts at: 675 1270 675
Size: 595 3298
BstBI TT'CG_AA
Cuts at: 636 2254 636
Size: 1618 2275
BstYI r'GATC_y
Cuts at: 1 830 918 1385 1408 1743 1989 3001 3087
Size: 829 88 467 23 335 246 1012 86
Cuts at: 3087 3099 3445 1
Size: 12 346 449
Fragments arranged by size:
1012 829 467 449 346 335 246 88
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 468 625 468
Size: 157 3736
BtgI C'CryG_G
Cuts at: 107 161 306 107
Size: 54 145 3694
Fragments arranged by size:
3694 145 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1843 2024 2187 1843
Size: 181 163 3549
Fragments arranged by size:
3549 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 1136
Size: 3893
DraI TTT'AAA
Cuts at: 892 892
Size: 3893
DrdI GACnn_nn'nnGTC
Cuts at: 1599 2457 1599
Size: 858 3035
EaeI y'GGCC_r
Cuts at: 164 1478 1652 2043 2070 3415 164
Size: 1314 174 391 27 1345 642
Fragments arranged by size:
1345 1314 642 391 174 27
EagI C'GGCC_G
Cuts at: 1478 1478
Size: 3893
EarI CTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2101 2411 2557 3703 2101
Size: 310 146 1146 2291
Fragments arranged by size:
2291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 153 249 905 153
Size: 96 656 3141
Fragments arranged by size:
3141 656 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 169 608 1718 2150 2897 3165 105
Size: 64 439 1110 432 747 268 833
Fragments arranged by size:
1110 833 747 439 432 268 64
EcoO109I rG'GnC_Cy
Cuts at: 1032 1032
Size: 3893
EcoRI G'AATT_C
Cuts at: 880 897 880
Size: 17 3876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 759 1912 759
Size: 1153 2740
FspI TGC'GCA
Cuts at: 1674 1674
Size: 3893
HaeII r_GCGC'y
Cuts at: 257 1327 1575 2597 3576 257
Size: 1070 248 1022 979 574
Fragments arranged by size:
1070 1022 979 574 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 430 462 1735 1765 1767 1797 103
Size: 32 295 32 1273 30 2 30 2199
Fragments arranged by size:
2199 1273 295 32 32 30 30 2
HincII GTy'rAC
Cuts at: 932 932
Size: 3893
KasI G'GCGC_C
Cuts at: 1323 1571 1323
Size: 248 3645
KpnI G_GTAC'C
Cuts at: 913 913
Size: 3893
MluI A'CGCG_T
Cuts at: 3326 3326
Size: 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2564 2748 2564
Size: 184 3709
MscI TGG'CCA
Cuts at: 1654 1654
Size: 3893
MslI CAynn'nnrTG
Cuts at: 166 396 520 574 1882 2009 166
Size: 230 124 54 1308 127 2050
Fragments arranged by size:
2050 1308 230 127 124 54
MspA1I CmG'CkG
Cuts at: 147 565 666 1004 1318 1678 2691 2936 147
Size: 418 101 338 314 360 1013 245 1104
Fragments arranged by size:
1104 1013 418 360 338 314 245 101
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 3893
NarI GG'CG_CC
Cuts at: 1324 1572 1324
Size: 248 3645
NcoI C'CATG_G
Cuts at: 107 161 306 107
Size: 54 145 3694
Fragments arranged by size:
3694 145 54
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 3893
NsiI A_TGCA'T
Cuts at: 754 754
Size: 3893
NspI r_CATG'y
Cuts at: 417 858 946 1446 2353 417
Size: 441 88 500 907 1957
Fragments arranged by size:
1957 907 500 441 88
PasI CC'CwG_GG
Cuts at: 455 455
Size: 3893
PciI A'CATG_T
Cuts at: 413 2349 413
Size: 1936 1957
PmeI GTTT'AAAC
Cuts at: 892 892
Size: 3893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 538 570 3032 3064 538
Size: 32 2462 32 1367
Fragments arranged by size:
2462 1367 32 32
PstI C_TGCA'G
Cuts at: 146 659 940 1625 146
Size: 513 281 685 2414
Fragments arranged by size:
2414 685 513 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3893
PvuII CAG'CTG
Cuts at: 147 1318 1678 147
Size: 1171 360 2362
Fragments arranged by size:
2362 1171 360
RsrII CG'GwC_CG
Cuts at: 2088 2088
Size: 3893
SacI G_AGCT'C
Cuts at: 155 251 907 155
Size: 96 656 3141
Fragments arranged by size:
3141 656 96
SalI G'TCGA_C
Cuts at: 930 930
Size: 3893
SapI GCTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
SbfI CC_TGCA'GG
Cuts at: 940 940
Size: 3893
ScaI AGT'ACT
Cuts at: 3410 3410
Size: 3893
SfcI C'TryA_G
Cuts at: 33 142 655 693 936 1621 2614 2805 33
Size: 109 513 38 243 685 993 191 1121
Fragments arranged by size:
1121 993 685 513 243 191 109 38
SfoI GGC'GCC
Cuts at: 1325 1573 1325
Size: 248 3645
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
SmaI CCC'GGG
Cuts at: 915 915
Size: 3893
SmlI C'TyrA_G
Cuts at: 1048 1963 2455 2717 1048
Size: 915 492 262 2224
Fragments arranged by size:
2224 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3893
SphI G_CATG'C
Cuts at: 946 1446 946
Size: 500 3393
StuI AGG'CCT
Cuts at: 466 466
Size: 3893
StyI C'CwwG_G
Cuts at: 107 161 306 518 1027 1381 107
Size: 54 145 212 509 354 2619
Fragments arranged by size:
2619 509 354 212 145 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 319 1476 1609 2068 2277 319
Size: 1157 133 459 209 1935
Fragments arranged by size:
1935 1157 459 209 133
TatI w'GTAC_w
Cuts at: 857 3408 857
Size: 2551 1342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 3861
Tth111I GACn'n_nGTC
Cuts at: 1690 1690
Size: 3893
XbaI T'CTAG_A
Cuts at: 265 924 265
Size: 659 3234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 1388 390
Size: 998 2895
XmaI C'CCGG_G
Cuts at: 913 913
Size: 3893
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BcgI BmgBI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI