Restriction map for
pF1KB8157
(Circular) (Six-base) MAPSORT of: pF1KB8157.seq Check: 4680 from: 1 to: 4760
pF1KB8157 4760 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1370 1684 1798 1370
Size: 314 114 4332
Fragments arranged by size:
4332 314 114
Acc65I G'GTAC_C
Cuts at: 654 1776 654
Size: 1122 3638
AclI AA'CG_TT
Cuts at: 4600 4600
Size: 4760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1722 2585 3017 3764 4032 1722
Size: 863 432 747 268 2450
Fragments arranged by size:
2450 863 747 432 268
AflIII A'CryG_T
Cuts at: 2112 3216 4193 2112
Size: 1104 977 2679
Fragments arranged by size:
2679 1104 977
AgeI A'CCGG_T
Cuts at: 3148 3148
Size: 4760
AlwNI CAG_nnn'CTG
Cuts at: 2065 3632 2065
Size: 1567 3193
ApaI G_GGCC'C
Cuts at: 1444 1444
Size: 4760
ApaLI G'TGCA_C
Cuts at: 3530 3530
Size: 4760
ApoI r'AATT_y
Cuts at: 480 666 1185 1195 1764 4113 4369 480
Size: 186 519 10 569 2349 256 871
Fragments arranged by size:
2349 871 569 519 256 186 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 4760
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
AvaI C'yCGr_G
Cuts at: 1780 1780
Size: 4760
AvrII C'CTAG_G
Cuts at: 238 238
Size: 4760
BamHI G'GATC_C
Cuts at: 1785 1785
Size: 4760
BanI G'GyrC_C
Cuts at: 654 1294 1776 2190 2438 2473 654
Size: 640 482 414 248 35 2941
Fragments arranged by size:
2941 640 482 414 248 35
BanII G_rGCy'C
Cuts at: 1444 1774 2804 1444
Size: 330 1030 3400
Fragments arranged by size:
3400 1030 330
BbeI G_GCGC'C
Cuts at: 2194 2442 2194
Size: 248 4512
BbsI GAAGACnn'nnnn_
Cuts at: 1466 1466
Size: 4760
BciVI GTATCCnnnnn_n'
Cuts at: 969 2658 3419 4760 969
Size: 1689 761 1341 969
Fragments arranged by size:
1689 1341 969 761
BclI T'GATC_A
Cuts at: 2280 2280
Size: 4760
BglII A'GATC_T
Cuts at: 648 2275 4312 648
Size: 1627 2037 1096
Fragments arranged by size:
2037 1627 1096
BlpI GC'TnA_GC
Cuts at: 299 1872 299
Size: 1573 3187
Bme1580I G_kGCm'C
Cuts at: 1444 2385 2478 3534 1444
Size: 941 93 1056 2670
Fragments arranged by size:
2670 1056 941 93
BmrI ACTGGGnnnn_n'
Cuts at: 1972 2053 2388 4532 1972
Size: 81 335 2144 2200
Fragments arranged by size:
2200 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 4760
Bpu10I CC'TnA_GC
Cuts at: 304 1617 304
Size: 1313 3447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1936 2815 3307 3605 1936
Size: 879 492 298 3091
Fragments arranged by size:
3091 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4464 4464
Size: 4760
BsaAI yAC'GTr
Cuts at: 2113 2113
Size: 4760
BsaBI GATnn'nnATC
Cuts at: 6 1012 2274 4311 6
Size: 1006 1262 2037 455
Fragments arranged by size:
2037 1262 1006 455
BsaHI Gr'CG_yC
Cuts at: 2191 2439 3141 4637 2191
Size: 248 702 1496 2314
Fragments arranged by size:
2314 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1477 1949 2470 3148 3422 3569 3980 1477
Size: 472 521 678 274 147 411 2257
Fragments arranged by size:
2257 678 521 472 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1326 1356 1639 1669 1326
Size: 30 283 30 4417
Fragments arranged by size:
4417 283 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1047 1328 1331 1567 1675 1047
Size: 281 3 236 108 4132
Fragments arranged by size:
4132 281 236 108 3
BseYI C'CCAG_C
Cuts at: 2185 2943 3520 4016 2185
Size: 758 577 496 2929
Fragments arranged by size:
2929 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2302 2348 3556 104
Size: 2198 46 1208 1308
Fragments arranged by size:
2198 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 270 1774 2552 2742 3534 270
Size: 1504 778 190 792 1496
Fragments arranged by size:
1504 1496 792 778 190
BsmI GAATG_Cn'
Cuts at: 786 786
Size: 4760
Bsp1286I G_dGCh'C
Cuts at: 270 1444 1774 2385 2478 2552 2742 2804 3534
Size: 1174 330 611 93 74 190 62 730
Cuts at: 3534 270
Size: 1496
Fragments arranged by size:
1496 1174 730 611 330 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3980 3980
Size: 4760
BspHI T'CATG_A
Cuts at: 3936 3936
Size: 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 1663 1810 2024 2326 2707 157
Size: 1506 147 214 302 381 2210
Fragments arranged by size:
2210 1506 381 302 214 147
BsrBI CCG'CTC
Cuts at: 50 3052 3106 4590 4755 50
Size: 3002 54 1484 165 55
Fragments arranged by size:
3002 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2672 2672
Size: 4760
BsrFI r'CCGG_y
Cuts at: 2758 2939 3148 2758
Size: 181 209 4370
Fragments arranged by size:
4370 209 181
BssSI C'ACGA_G
Cuts at: 3031 3389 3031
Size: 358 4402
BstAPI GCAn_nnn'nTGC
Cuts at: 149 2137 149
Size: 1988 2772
BstBI TT'CG_AA
Cuts at: 3121 3121
Size: 4760
BstXI CCAn_nnnn'nTGG
Cuts at: 1240 1240
Size: 4760
BstYI r'GATC_y
Cuts at: 1 561 648 1785 2252 2275 2610 2856 3868
Size: 560 87 1137 467 23 335 246 1012
Cuts at: 3868 3954 3966 4312 1
Size: 86 12 346 449
Fragments arranged by size:
1137 1012 560 467 449 346 335 246
87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 584 584
Size: 4760
BtgI C'CryG_G
Cuts at: 962 962
Size: 4760
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2710 2891 3054 2710
Size: 181 163 4416
Fragments arranged by size:
4416 181 163
BtsI GCAGTG_nn'
Cuts at: 2003 2003
Size: 4760
DraI TTT'AAA
Cuts at: 1759 1759
Size: 4760
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3324 2466
Size: 858 3902
EaeI y'GGCC_r
Cuts at: 1451 2345 2519 2910 2937 4282 1451
Size: 894 174 391 27 1345 1929
Fragments arranged by size:
1929 1345 894 391 174 27
EagI C'GGCC_G
Cuts at: 2345 2345
Size: 4760
EarI CTCTTCn'nnn_
Cuts at: 290 587 1215 1631 1712 2783 2993 290
Size: 297 628 416 81 1071 210 2057
Fragments arranged by size:
2057 1071 628 416 297 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2968 3278 3424 4570 2968
Size: 310 146 1146 3158
Fragments arranged by size:
3158 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1772 1772
Size: 4760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1722 2585 3017 3764 4032 1722
Size: 863 432 747 268 2450
Fragments arranged by size:
2450 863 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1132 1132
Size: 4760
EcoO109I rG'GnC_Cy
Cuts at: 822 1440 1441 1899 822
Size: 618 1 458 3683
Fragments arranged by size:
3683 618 458 1
EcoRI G'AATT_C
Cuts at: 480 1185 1764 480
Size: 705 579 3476
Fragments arranged by size:
3476 705 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 841 2779 841
Size: 1938 2822
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4760
HaeII r_GCGC'y
Cuts at: 2194 2442 3464 4443 2194
Size: 248 1022 979 2511
Fragments arranged by size:
2511 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1326 1358 1639 1671 2602 2632 2634 2664 1326
Size: 32 281 32 931 30 2 30 3422
Fragments arranged by size:
3422 931 281 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1678 1799 1678
Size: 121 4639
KasI G'GCGC_C
Cuts at: 2190 2438 2190
Size: 248 4512
KpnI G_GTAC'C
Cuts at: 658 1780 658
Size: 1122 3638
MluI A'CGCG_T
Cuts at: 4193 4193
Size: 4760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 530 858 3431 3615 530
Size: 328 2573 184 1675
Fragments arranged by size:
2573 1675 328 184
MscI TGG'CCA
Cuts at: 1453 2521 1453
Size: 1068 3692
MslI CAynn'nnrTG
Cuts at: 2749 2876 2749
Size: 127 4633
MspA1I CmG'CkG
Cuts at: 1871 2185 2545 3558 3803 1871
Size: 314 360 1013 245 2828
Fragments arranged by size:
2828 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2941 2941
Size: 4760
NarI GG'CG_CC
Cuts at: 2191 2439 2191
Size: 248 4512
NcoI C'CATG_G
Cuts at: 962 962
Size: 4760
NgoMIV G'CCGG_C
Cuts at: 2939 2939
Size: 4760
NspI r_CATG'y
Cuts at: 984 1626 1813 2313 3220 984
Size: 642 187 500 907 2524
Fragments arranged by size:
2524 907 642 500 187
PciI A'CATG_T
Cuts at: 3216 3216
Size: 4760
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4728
PspOMI G'GGCC_C
Cuts at: 1440 1440
Size: 4760
PstI C_TGCA'G
Cuts at: 1635 1807 2492 1635
Size: 172 685 3903
Fragments arranged by size:
3903 685 172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4760
PvuII CAG'CTG
Cuts at: 2185 2545 2185
Size: 360 4400
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4760
SacI G_AGCT'C
Cuts at: 1774 1774
Size: 4760
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4760
SapI GCTCTTCn'nnn_
Cuts at: 290 2783 2993 290
Size: 2493 210 2057
Fragments arranged by size:
2493 2057 210
SbfI CC_TGCA'GG
Cuts at: 1807 1807
Size: 4760
ScaI AGT'ACT
Cuts at: 1157 4277 1157
Size: 3120 1640
SfcI C'TryA_G
Cuts at: 33 1179 1631 1667 1803 2488 3481 3672 33
Size: 1146 452 36 136 685 993 191 1121
Fragments arranged by size:
1146 1121 993 685 452 191 136 36
SfoI GGC'GCC
Cuts at: 2192 2440 2192
Size: 248 4512
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4760
SmlI C'TyrA_G
Cuts at: 1915 2830 3322 3584 1915
Size: 915 492 262 3091
Fragments arranged by size:
3091 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4760
SphI G_CATG'C
Cuts at: 984 1813 2313 984
Size: 829 500 3431
Fragments arranged by size:
3431 829 500
StuI AGG'CCT
Cuts at: 117 117
Size: 4760
StyI C'CwwG_G
Cuts at: 238 962 995 1894 2248 238
Size: 724 33 899 354 2750
Fragments arranged by size:
2750 899 724 354 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2343 2476 2935 3144 2343
Size: 133 459 209 3959
Fragments arranged by size:
3959 459 209 133
TatI w'GTAC_w
Cuts at: 494 1155 1741 4275 494
Size: 661 586 2534 979
Fragments arranged by size:
2534 979 661 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4277 4309 4277
Size: 32 4728
Tth111I GACn'n_nGTC
Cuts at: 2557 2557
Size: 4760
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2255 2255
Size: 4760
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4760
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI