Restriction map for
pF1KB8144
(Circular) (Six-base) MAPSORT of: pF1KB8144.seq Check: 9069 from: 1 to: 3920
pF1KB8144 3920 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 139 139
Size: 3920
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1745 2177 2924 3192 142
Size: 1603 432 747 268 870
Fragments arranged by size:
1603 870 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AleI CACnn'nnGTG
Cuts at: 663 663
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 1225 2792 1225
Size: 1567 2353
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 368 766 924 3273 3529 368
Size: 398 158 2349 256 759
Fragments arranged by size:
2349 759 398 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 243 940 243
Size: 697 3223
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 936 1350 1598 1633 936
Size: 414 248 35 3223
Fragments arranged by size:
3223 414 248 35
BanII G_rGCy'C
Cuts at: 934 1964 934
Size: 1030 2890
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 465 465
Size: 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 634 668 90
Size: 34 510 34 3342
Fragments arranged by size:
3342 510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglI GCCn_nnn'nGGC
Cuts at: 218 218
Size: 3920
BglII A'GATC_T
Cuts at: 738 1435 3472 738
Size: 697 2037 1186
Fragments arranged by size:
2037 1186 697
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 1545 1638 2694 1545
Size: 93 1056 2771
Fragments arranged by size:
2771 1056 93
BmgBI CAC'GTC
Cuts at: 152 665 152
Size: 513 3407
BmrI ACTGGGnnnn_n'
Cuts at: 272 1132 1213 1548 3692 272
Size: 860 81 335 2144 500
Fragments arranged by size:
2144 860 500 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 401 2287 401
Size: 1886 2034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 275
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 689 3624 689
Size: 2935 985
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 114 1345 2103 2680 3176 114
Size: 1231 758 577 496 858
Fragments arranged by size:
1231 858 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 934 1545 1638 1712 1902 1964 2694 934
Size: 611 93 74 190 62 730 2160
Fragments arranged by size:
2160 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 139 970 1184 1486 1867 139
Size: 831 214 302 381 2192
Fragments arranged by size:
2192 831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 201 1832 201
Size: 1631 2289
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 140 1297 140
Size: 1157 2763
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstXI CCAn_nnnn'nTGG
Cuts at: 114 912 114
Size: 798 3122
BstYI r'GATC_y
Cuts at: 1 188 738 945 1412 1435 1770 2016 3028
Size: 187 550 207 467 23 335 246 1012
Cuts at: 3028 3114 3126 3472 1
Size: 86 12 346 449
Fragments arranged by size:
1012 550 467 449 346 335 246 207
187 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 518 1163 518
Size: 645 3275
DraI TTT'AAA
Cuts at: 471 919 471
Size: 448 3472
DrdI GACnn_nn'nnGTC
Cuts at: 153 1626 2484 153
Size: 1473 858 1589
Fragments arranged by size:
1589 1473 858
EaeI y'GGCC_r
Cuts at: 210 1505 1679 2070 2097 3442 210
Size: 1295 174 391 27 1345 688
Fragments arranged by size:
1345 1295 688 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 643 1943 2153 643
Size: 1300 210 2410
Fragments arranged by size:
2410 1300 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 303 2128 2438 2584 3730 303
Size: 1825 310 146 1146 493
Fragments arranged by size:
1825 1146 493 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 275 1745 2177 2924 3192 142
Size: 133 1470 432 747 268 870
Fragments arranged by size:
1470 870 747 432 268 133
EcoO109I rG'GnC_Cy
Cuts at: 1059 1059
Size: 3920
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 1354 1602 2624 3603 1354
Size: 248 1022 979 1671
Fragments arranged by size:
1671 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 611 643 697 729 1762 1792 1794 1824 611
Size: 32 54 32 1033 30 2 30 2707
Fragments arranged by size:
2707 1033 54 32 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 663 1909 2036 663
Size: 1246 127 2547
Fragments arranged by size:
2547 1246 127
MspA1I CmG'CkG
Cuts at: 114 230 272 1031 1345 1705 2718 2963 114
Size: 116 42 759 314 360 1013 245 1071
Fragments arranged by size:
1071 1013 759 360 314 245 116 42
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NdeI CA'TA_TG
Cuts at: 853 853
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PfoI T'CCnGG_A
Cuts at: 629 629
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PstI C_TGCA'G
Cuts at: 136 525 967 1652 136
Size: 389 442 685 2404
Fragments arranged by size:
2404 685 442 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 1345 1705 1345
Size: 360 3560
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 132 521 963 1648 2641 2832 33
Size: 99 389 442 685 993 191 1121
Fragments arranged by size:
1121 993 685 442 389 191 99
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 745 1054 1408 745
Size: 309 354 3257
Fragments arranged by size:
3257 354 309
SwaI ATTT'AAAT
Cuts at: 471 471
Size: 3920
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 150 1717 150
Size: 1567 2353
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 205 1415 205
Size: 1210 2710
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
XmnI GAAnn'nnTTC
Cuts at: 740 740
Size: 3920
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI XhoI ZraI