Restriction map for
pF1KB8137
(Circular) (Six-base) MAPSORT of: pF1KB8137.seq Check: 4741 from: 1 to: 4148
pF1KB8137 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 909 1164 909
Size: 255 3893
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1973 2405 3152 3420 736
Size: 1237 432 747 268 1464
Fragments arranged by size:
1464 1237 747 432 268
AfeI AGC'GCT
Cuts at: 837 837
Size: 4148
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1025 1025
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 669 1453 3020 669
Size: 784 1567 1797
Fragments arranged by size:
1797 1567 784
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 277 807 1152 3501 3757 277
Size: 530 345 2349 256 668
Fragments arranged by size:
2349 668 530 345 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 615 704 1168 615
Size: 89 464 3595
Fragments arranged by size:
3595 464 89
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 459 492 459
Size: 33 4115
BamHI G'GATC_C
Cuts at: 1173 1173
Size: 4148
BanI G'GyrC_C
Cuts at: 909 1164 1578 1826 1861 909
Size: 255 414 248 35 3196
Fragments arranged by size:
3196 414 255 248 35
BanII G_rGCy'C
Cuts at: 1162 2192 1162
Size: 1030 3118
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BbsI GAAGACnn'nnnn_
Cuts at: 724 880 724
Size: 156 3992
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BfrBI ATG'CAT
Cuts at: 636 636
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 1773 1866 2922 1773
Size: 93 1056 2999
Fragments arranged by size:
2999 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1360 1441 1776 3920 1360
Size: 81 335 2144 1588
Fragments arranged by size:
2144 1588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1324 2203 2695 2993 1085
Size: 239 879 492 298 2240
Fragments arranged by size:
2240 879 492 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 1099 3852 1099
Size: 2753 1395
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 263 293 1579 1827 2529 4025 263
Size: 30 1286 248 702 1496 386
Fragments arranged by size:
1496 1286 702 386 248 30
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1048 1048
Size: 4148
BseYI C'CCAG_C
Cuts at: 488 838 1059 1573 2331 2908 3404 488
Size: 350 221 514 758 577 496 1232
Fragments arranged by size:
1232 758 577 514 496 350 221
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1162 1940 2130 2922 1162
Size: 778 190 792 2388
Fragments arranged by size:
2388 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1162 1773 1866 1940 2130 2192 2922 1162
Size: 611 93 74 190 62 730 2388
Fragments arranged by size:
2388 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 563 3324 563
Size: 2761 1387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 730 1198 1412 1714 2095 730
Size: 468 214 302 381 2783
Fragments arranged by size:
2783 468 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2060 2060
Size: 4148
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BsrGI T'GTAC_A
Cuts at: 520 520
Size: 4148
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstEII G'GTnAC_C
Cuts at: 396 483 396
Size: 87 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 459 1023 459
Size: 564 3584
BstYI r'GATC_y
Cuts at: 1 1173 1640 1663 1998 2244 3256 3342 3354
Size: 1172 467 23 335 246 1012 86 12
Cuts at: 3354 3700 1
Size: 346 449
Fragments arranged by size:
1172 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 479 1075 479
Size: 596 3552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1147 1147
Size: 4148
DrdI GACnn_nn'nnGTC
Cuts at: 1854 2712 1854
Size: 858 3290
EaeI y'GGCC_r
Cuts at: 1013 1733 1907 2298 2325 3670 1013
Size: 720 174 391 27 1345 1491
Fragments arranged by size:
1491 1345 720 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 746 2356 2666 2812 3958 746
Size: 1610 310 146 1146 936
Fragments arranged by size:
1610 1146 936 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1973 2405 3152 3420 736
Size: 1237 432 747 268 1464
Fragments arranged by size:
1464 1237 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 353 1287 353
Size: 934 3214
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 741 2167 741
Size: 1426 2722
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 839 1582 1830 2852 3831 839
Size: 743 248 1022 979 1156
Fragments arranged by size:
1156 1022 979 743 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 329 361 596 628 738 770 1096 1128 1990
Size: 32 235 32 110 32 326 32 862
Cuts at: 1990 2020 2022 2052 329
Size: 30 2 30 2425
Fragments arranged by size:
2425 862 326 235 110 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1187 1187
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 913 1168 913
Size: 255 3893
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 851 2819 3003 851
Size: 1968 184 1996
Fragments arranged by size:
1996 1968 184
MscI TGG'CCA
Cuts at: 1909 1909
Size: 4148
MslI CAynn'nnrTG
Cuts at: 457 641 2137 2264 457
Size: 184 1496 127 2341
Fragments arranged by size:
2341 1496 184 127
MspA1I CmG'CkG
Cuts at: 883 1259 1573 1933 2946 3191 883
Size: 376 314 360 1013 245 1840
Fragments arranged by size:
1840 1013 376 360 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NcoI C'CATG_G
Cuts at: 479 479
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NsiI A_TGCA'T
Cuts at: 638 638
Size: 4148
NspI r_CATG'y
Cuts at: 1201 1701 2608 1201
Size: 500 907 2741
Fragments arranged by size:
2741 907 500
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 353 353
Size: 4148
PshAI GACnn'nnGTC
Cuts at: 225 225
Size: 4148
PstI C_TGCA'G
Cuts at: 227 1195 1880 227
Size: 968 685 2495
Fragments arranged by size:
2495 968 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 883 1573 1933 883
Size: 690 360 3098
Fragments arranged by size:
3098 690 360
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 223 1191 1876 2869 3060 33
Size: 190 968 685 993 191 1121
Fragments arranged by size:
1121 993 968 685 191 190
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 1100 1303 2218 2710 2972 1100
Size: 203 915 492 262 2276
Fragments arranged by size:
2276 915 492 262 203
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
SspI AAT'ATT
Cuts at: 650 650
Size: 4148
StyI C'CwwG_G
Cuts at: 479 512 1282 1636 479
Size: 33 770 354 2991
Fragments arranged by size:
2991 770 354 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 415 502 1731 1864 2323 2532 415
Size: 87 1229 133 459 209 2031
Fragments arranged by size:
2031 1229 459 209 133 87
TatI w'GTAC_w
Cuts at: 520 3663 520
Size: 3143 1005
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 433 465 3665 3697 433
Size: 32 3200 32 884
Fragments arranged by size:
3200 884 32 32
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI