Restriction map for
pF1KB8129
(Circular) (Six-base) MAPSORT of: pF1KB8129.seq Check: 7089 from: 1 to: 4136
pF1KB8129 4136 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 207 207
Size: 4136
AccI GT'mk_AC
Cuts at: 876 1174 876
Size: 298 3838
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 625 1961 2393 3140 3408 206
Size: 419 1336 432 747 268 934
Fragments arranged by size:
1336 934 747 432 419 268
AflIII A'CryG_T
Cuts at: 868 1488 2592 3569 868
Size: 620 1104 977 1435
Fragments arranged by size:
1435 1104 977 620
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AhdI GACnn_n'nnGTC
Cuts at: 872 872
Size: 4136
AlwNI CAG_nnn'CTG
Cuts at: 307 1002 1065 1102 1441 3008 307
Size: 695 63 37 339 1567 1435
Fragments arranged by size:
1567 1435 695 339 63 37
ApaLI G'TGCA_C
Cuts at: 423 2906 423
Size: 2483 1653
ApoI r'AATT_y
Cuts at: 649 1140 3489 3745 649
Size: 491 2349 256 1040
Fragments arranged by size:
2349 1040 491 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 362 854 1156 362
Size: 492 302 3342
Fragments arranged by size:
3342 492 302
AvrII C'CTAG_G
Cuts at: 123 123
Size: 4136
BamHI G'GATC_C
Cuts at: 119 1161 119
Size: 1042 3094
BanI G'GyrC_C
Cuts at: 1152 1566 1814 1849 1152
Size: 414 248 35 3439
Fragments arranged by size:
3439 414 248 35
BanII G_rGCy'C
Cuts at: 280 1150 2180 280
Size: 870 1030 2236
Fragments arranged by size:
2236 1030 870
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BbsI GAAGACnn'nnnn_
Cuts at: 770 770
Size: 4136
BbvCI CC'TCA_GC
Cuts at: 619 619
Size: 4136
BciVI GTATCCnnnnn_n'
Cuts at: 2034 2795 4136 2034
Size: 761 1341 2034
Fragments arranged by size:
2034 1341 761
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BglII A'GATC_T
Cuts at: 1651 3688 1651
Size: 2037 2099
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 427 555 891 1761 1854 2910 427
Size: 128 336 870 93 1056 1653
Fragments arranged by size:
1653 1056 870 336 128 93
BmrI ACTGGGnnnn_n'
Cuts at: 607 1348 1429 1764 3908 607
Size: 741 81 335 2144 835
Fragments arranged by size:
2144 835 741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1128 2503 1128
Size: 1375 2761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 220 368 395 499 1075 1079 196
Size: 24 148 27 104 576 4 3253
Fragments arranged by size:
3253 576 148 104 27 24 4
Bpu10I CC'TnA_GC
Cuts at: 619 619
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 940 1312 2191 2683 2981 901
Size: 39 372 879 492 298 2056
Fragments arranged by size:
2056 879 492 372 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 871 1489 871
Size: 618 3518
BsaBI GATnn'nnATC
Cuts at: 6 151 1650 3687 6
Size: 145 1499 2037 455
Fragments arranged by size:
2037 1499 455 145
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1325 1846 2524 2798 2945 3356 1325
Size: 521 678 274 147 411 2105
Fragments arranged by size:
2105 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 341 371 341
Size: 30 4106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 850 1138 850
Size: 288 3848
BseYI C'CCAG_C
Cuts at: 543 587 1036 1561 2319 2896 3392 543
Size: 44 449 525 758 577 496 1287
Fragments arranged by size:
1287 758 577 525 496 449 44
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 427 1150 1928 2118 2910 427
Size: 723 778 190 792 1653
Fragments arranged by size:
1653 792 778 723 190
BsmBI CGTCTCn'nnnn_
Cuts at: 505 505
Size: 4136
Bsp1286I G_dGCh'C
Cuts at: 280 427 555 891 1150 1761 1854 1928 2118
Size: 147 128 336 259 611 93 74 190
Cuts at: 2118 2180 2910 280
Size: 62 730 1506
Fragments arranged by size:
1506 730 611 336 259 190 147 128
93 74 62
BspEI T'CCGG_A
Cuts at: 3356 3356
Size: 4136
BspHI T'CATG_A
Cuts at: 773 3312 773
Size: 2539 1597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 207 1119 1186 1400 1702 2083 207
Size: 912 67 214 302 381 2260
Fragments arranged by size:
2260 912 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2428 2482 3966 4131 50
Size: 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 368 2048 368
Size: 1680 2456
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 1002 1065 1513 1002
Size: 63 448 3625
Fragments arranged by size:
3625 448 63
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstEII G'GTnAC_C
Cuts at: 747 747
Size: 4136
BstXI CCAn_nnnn'nTGG
Cuts at: 759 759
Size: 4136
BstYI r'GATC_y
Cuts at: 1 119 146 1161 1628 1651 1986 2232 3244
Size: 118 27 1015 467 23 335 246 1012
Cuts at: 3244 3330 3342 3688 1
Size: 86 12 346 449
Fragments arranged by size:
1015 1012 467 449 346 335 246 118
86 27 23 12
BtgI C'CryG_G
Cuts at: 156 758 156
Size: 602 3534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1379
Size: 4136
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DraIII CAC_nnn'GTG
Cuts at: 601 601
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 309 944 1721 1895 2286 2313 3658 309
Size: 635 777 174 391 27 1345 787
Fragments arranged by size:
1345 787 777 635 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 1012 2159 2369 1012
Size: 1147 210 2779
Fragments arranged by size:
2779 1147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1014 2344 2654 2800 3946 1014
Size: 1330 310 146 1146 1204
Fragments arranged by size:
1330 1204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 206 220 368 395 499 625 1075 1079
Size: 10 14 148 27 104 126 450 4
Cuts at: 1079 1961 2393 3140 3408 196
Size: 882 432 747 268 924
Fragments arranged by size:
924 882 747 450 432 268 148 126
104 27 14 10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 127 214 127
Size: 87 4049
EcoO109I rG'GnC_Cy
Cuts at: 207 232 850 1275 207
Size: 25 618 425 3068
Fragments arranged by size:
3068 618 425 25
EcoRI G'AATT_C
Cuts at: 1140 1140
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 4136
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1570 1818 2840 3819 1570
Size: 248 1022 979 1887
Fragments arranged by size:
1887 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 858 890 1978 2008 2010 2040 224
Size: 32 602 32 1088 30 2 30 2320
Fragments arranged by size:
2320 1088 602 32 32 30 30 2
HincII GTy'rAC
Cuts at: 877 1175 877
Size: 298 3838
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2991 2807
Size: 184 3952
MscI TGG'CCA
Cuts at: 946 1897 946
Size: 951 3185
MslI CAynn'nnrTG
Cuts at: 772 778 2125 2252 772
Size: 6 1347 127 2656
Fragments arranged by size:
2656 1347 127 6
MspA1I CmG'CkG
Cuts at: 543 999 1053 1247 1561 1921 2934 3179 543
Size: 456 54 194 314 360 1013 245 1500
Fragments arranged by size:
1500 1013 456 360 314 245 194 54
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NcoI C'CATG_G
Cuts at: 758 758
Size: 4136
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 595 1189 1689 2596 595
Size: 594 500 907 2135
Fragments arranged by size:
2135 907 594 500
PciI A'CATG_T
Cuts at: 2592 2592
Size: 4136
PfoI T'CCnGG_A
Cuts at: 962 1095 962
Size: 133 4003
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PmlI CAC'GTG
Cuts at: 871 871
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 4104
PpuMI rG'GwC_Cy
Cuts at: 207 232 207
Size: 25 4111
PstI C_TGCA'G
Cuts at: 494 542 584 1070 1183 1868 494
Size: 48 42 486 113 685 2762
Fragments arranged by size:
2762 685 486 113 48 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 543 999 1053 1561 1921 543
Size: 456 54 508 360 2758
Fragments arranged by size:
2758 508 456 360 54
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 875 1173 875
Size: 298 3838
SapI GCTCTTCn'nnn_
Cuts at: 1012 2159 2369 1012
Size: 1147 210 2779
Fragments arranged by size:
2779 1147 210
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 3653 3653
Size: 4136
SfcI C'TryA_G
Cuts at: 33 490 538 570 580 1066 1179 1864 2857
Size: 457 48 32 10 486 113 685 993
Cuts at: 2857 3048 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 486 457 191 113 48
32 10
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 856 1158 856
Size: 302 3834
SmlI C'TyrA_G
Cuts at: 880 955 1291 2206 2698 2960 880
Size: 75 336 915 492 262 2056
Fragments arranged by size:
2056 915 492 336 262 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 595 1189 1689 595
Size: 594 500 3042
Fragments arranged by size:
3042 594 500
StyI C'CwwG_G
Cuts at: 123 758 1270 1624 123
Size: 635 512 354 2635
Fragments arranged by size:
2635 635 512 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 589 1719 1852 2311 2520 589
Size: 1130 133 459 209 2205
Fragments arranged by size:
2205 1130 459 209 133
TatI w'GTAC_w
Cuts at: 3651 3651
Size: 4136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 341 373 3653 3685 341
Size: 32 3280 32 792
Fragments arranged by size:
3280 792 32 32
Tth111I GACn'n_nGTC
Cuts at: 391 1933 391
Size: 1542 2594
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1631 1631
Size: 4136
XmaI C'CCGG_G
Cuts at: 854 1156 854
Size: 302 3834
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI