Restriction map for
pF1KB8109
(Circular) (Six-base) MAPSORT of: pF1KB8109.seq Check: 9851 from: 1 to: 3572
pF1KB8109 3572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 335 610 335
Size: 275 3297
Acc65I G'GTAC_C
Cuts at: 588 588
Size: 3572
AclI AA'CG_TT
Cuts at: 3412 3412
Size: 3572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1397 1829 2576 2844 530
Size: 867 432 747 268 1258
Fragments arranged by size:
1258 867 747 432 268
AflIII A'CryG_T
Cuts at: 924 2028 3005 924
Size: 1104 977 1491
Fragments arranged by size:
1491 1104 977
AgeI A'CCGG_T
Cuts at: 1960 1960
Size: 3572
AlwNI CAG_nnn'CTG
Cuts at: 877 2444 877
Size: 1567 2005
ApaLI G'TGCA_C
Cuts at: 2342 2342
Size: 3572
ApoI r'AATT_y
Cuts at: 576 2925 3181 576
Size: 2349 256 967
Fragments arranged by size:
2349 967 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
AvaI C'yCGr_G
Cuts at: 248 592 248
Size: 344 3228
BamHI G'GATC_C
Cuts at: 597 597
Size: 3572
BanI G'GyrC_C
Cuts at: 534 588 1002 1250 1285 534
Size: 54 414 248 35 2821
Fragments arranged by size:
2821 414 248 54 35
BanII G_rGCy'C
Cuts at: 586 1616 586
Size: 1030 2542
BbeI G_GCGC'C
Cuts at: 1006 1254 1006
Size: 248 3324
BbsI GAAGACnn'nnnn_
Cuts at: 184 195 184
Size: 11 3561
BciVI GTATCCnnnnn_n'
Cuts at: 1470 2231 3572 1470
Size: 761 1341 1470
Fragments arranged by size:
1470 1341 761
BclI T'GATC_A
Cuts at: 1092 1092
Size: 3572
BglII A'GATC_T
Cuts at: 278 1087 3124 278
Size: 809 2037 726
Fragments arranged by size:
2037 809 726
BlpI GC'TnA_GC
Cuts at: 684 684
Size: 3572
Bme1580I G_kGCm'C
Cuts at: 361 537 1197 1290 2346 361
Size: 176 660 93 1056 1587
Fragments arranged by size:
1587 1056 660 176 93
BmrI ACTGGGnnnn_n'
Cuts at: 784 865 1200 3344 784
Size: 81 335 2144 1012
Fragments arranged by size:
2144 1012 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1627 2119 2417 748
Size: 879 492 298 1903
Fragments arranged by size:
1903 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 552 3276 552
Size: 2724 848
BsaAI yAC'GTr
Cuts at: 925 925
Size: 3572
BsaBI GATnn'nnATC
Cuts at: 6 1086 3123 6
Size: 1080 2037 455
Fragments arranged by size:
2037 1080 455
BsaHI Gr'CG_yC
Cuts at: 1003 1251 1953 3449 1003
Size: 248 702 1496 1126
Fragments arranged by size:
1496 1126 702 248
BsaWI w'CCGG_w
Cuts at: 761 1282 1960 2234 2381 2792 761
Size: 521 678 274 147 411 1541
Fragments arranged by size:
1541 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 229
Size: 30 3542
BseYI C'CCAG_C
Cuts at: 997 1755 2332 2828 997
Size: 758 577 496 1741
Fragments arranged by size:
1741 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 167 171 222 1114 1160 2368 104
Size: 63 4 51 892 46 1208 1308
Fragments arranged by size:
1308 1208 892 63 51 46 4
BsiHKAI G_wGCw'C
Cuts at: 586 1364 1554 2346 586
Size: 778 190 792 1812
Fragments arranged by size:
1812 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 361 537 586 1197 1290 1364 1554 1616 2346
Size: 176 49 611 93 74 190 62 730
Cuts at: 2346 361
Size: 1587
Fragments arranged by size:
1587 730 611 190 176 93 74 62
49
BspEI T'CCGG_A
Cuts at: 2792 2792
Size: 3572
BspHI T'CATG_A
Cuts at: 2748 2748
Size: 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 836 1138 1519 622
Size: 214 302 381 2675
Fragments arranged by size:
2675 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1864 1918 3402 3567 50
Size: 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1484 1484
Size: 3572
BsrFI r'CCGG_y
Cuts at: 1570 1751 1960 1570
Size: 181 209 3182
Fragments arranged by size:
3182 209 181
BssSI C'ACGA_G
Cuts at: 1843 2201 1843
Size: 358 3214
BstAPI GCAn_nnn'nTGC
Cuts at: 949 949
Size: 3572
BstBI TT'CG_AA
Cuts at: 1933 1933
Size: 3572
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3572
BstYI r'GATC_y
Cuts at: 1 278 597 1064 1087 1422 1668 2680 2766
Size: 277 319 467 23 335 246 1012 86
Cuts at: 2766 2778 3124 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 319 277 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3572
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1522 1703 1866 1522
Size: 181 163 3228
Fragments arranged by size:
3228 181 163
BtsI GCAGTG_nn'
Cuts at: 815 815
Size: 3572
DraI TTT'AAA
Cuts at: 571 571
Size: 3572
DrdI GACnn_nn'nnGTC
Cuts at: 474 1278 2136 474
Size: 804 858 1910
Fragments arranged by size:
1910 858 804
EaeI y'GGCC_r
Cuts at: 219 1157 1331 1722 1749 3094 219
Size: 938 174 391 27 1345 697
Fragments arranged by size:
1345 938 697 391 174 27
EagI C'GGCC_G
Cuts at: 219 1157 219
Size: 938 2634
EarI CTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1780 2090 2236 3382 1780
Size: 310 146 1146 1970
Fragments arranged by size:
1970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 584 584
Size: 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1397 1829 2576 2844 530
Size: 867 432 747 268 1258
Fragments arranged by size:
1258 867 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 545 545
Size: 3572
EcoO109I rG'GnC_Cy
Cuts at: 711 711
Size: 3572
EcoRI G'AATT_C
Cuts at: 576 576
Size: 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1591 1591
Size: 3572
FspI TGC'GCA
Cuts at: 1353 1353
Size: 3572
HaeII r_GCGC'y
Cuts at: 1006 1254 2276 3255 1006
Size: 248 1022 979 1323
Fragments arranged by size:
1323 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 1414 1444 1446 1476 229
Size: 32 1153 30 2 30 2325
Fragments arranged by size:
2325 1153 32 30 30 2
HincII GTy'rAC
Cuts at: 611 611
Size: 3572
HindIII A'AGCT_T
Cuts at: 318 318
Size: 3572
KasI G'GCGC_C
Cuts at: 1002 1250 1002
Size: 248 3324
KpnI G_GTAC'C
Cuts at: 592 592
Size: 3572
MluI A'CGCG_T
Cuts at: 3005 3005
Size: 3572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2427 2243
Size: 184 3388
MscI TGG'CCA
Cuts at: 1333 1333
Size: 3572
MslI CAynn'nnrTG
Cuts at: 112 289 1561 1688 112
Size: 177 1272 127 1996
Fragments arranged by size:
1996 1272 177 127
MspA1I CmG'CkG
Cuts at: 683 997 1357 2370 2615 683
Size: 314 360 1013 245 1640
Fragments arranged by size:
1640 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1753 1753
Size: 3572
NarI GG'CG_CC
Cuts at: 1003 1251 1003
Size: 248 3324
NcoI C'CATG_G
Cuts at: 107 107
Size: 3572
NdeI CA'TA_TG
Cuts at: 564 564
Size: 3572
NgoMIV G'CCGG_C
Cuts at: 1751 1751
Size: 3572
NspI r_CATG'y
Cuts at: 185 625 1125 2032 185
Size: 440 500 907 1725
Fragments arranged by size:
1725 907 500 440
PciI A'CATG_T
Cuts at: 2028 2028
Size: 3572
PflMI CCAn_nnn'nTGG
Cuts at: 234 234
Size: 3572
PmeI GTTT'AAAC
Cuts at: 571 571
Size: 3572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 3540
PstI C_TGCA'G
Cuts at: 476 619 1304 476
Size: 143 685 2744
Fragments arranged by size:
2744 685 143
PvuI CG_AT'CG
Cuts at: 104 167 104
Size: 63 3509
PvuII CAG'CTG
Cuts at: 997 1357 997
Size: 360 3212
RsrII CG'GwC_CG
Cuts at: 1767 1767
Size: 3572
SacI G_AGCT'C
Cuts at: 586 586
Size: 3572
SalI G'TCGA_C
Cuts at: 609 609
Size: 3572
SapI GCTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
SbfI CC_TGCA'GG
Cuts at: 619 619
Size: 3572
ScaI AGT'ACT
Cuts at: 3089 3089
Size: 3572
SfcI C'TryA_G
Cuts at: 33 472 615 1300 2293 2484 33
Size: 439 143 685 993 191 1121
Fragments arranged by size:
1121 993 685 439 191 143
SfoI GGC'GCC
Cuts at: 1004 1252 1004
Size: 248 3324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
SmaI CCC'GGG
Cuts at: 250 594 250
Size: 344 3228
SmlI C'TyrA_G
Cuts at: 727 1642 2134 2396 727
Size: 915 492 262 1903
Fragments arranged by size:
1903 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3572
SphI G_CATG'C
Cuts at: 625 1125 625
Size: 500 3072
StyI C'CwwG_G
Cuts at: 107 706 1060 107
Size: 599 354 2619
Fragments arranged by size:
2619 599 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1288 1747 1956 1155
Size: 133 459 209 2771
Fragments arranged by size:
2771 459 209 133
TatI w'GTAC_w
Cuts at: 3087 3087
Size: 3572
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3540
Tth111I GACn'n_nGTC
Cuts at: 1369 1369
Size: 3572
XbaI T'CTAG_A
Cuts at: 603 603
Size: 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1067 1067
Size: 3572
XmaI C'CCGG_G
Cuts at: 248 592 248
Size: 344 3228
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI