(Circular) (Six-base) MAPSORT of: pF1KB8108.seq Check: 1909 from: 1 to: 4547 pF1KB8108 4547 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 1585 1585 Size: 4547 Acc65I G'GTAC_C Cuts at: 1563 1563 Size: 4547 AclI AA'CG_TT Cuts at: 4387 4387 Size: 4547 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1423 2372 2804 3551 3819 1423 Size: 949 432 747 268 2151 Fragments arranged by size: 2151 949 747 432 268 AflIII A'CryG_T Cuts at: 1278 1507 1899 3003 3980 1278 Size: 229 392 1104 977 1845 Fragments arranged by size: 1845 1104 977 392 229 AgeI A'CCGG_T Cuts at: 2935 2935 Size: 4547 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 422 454 974 1006 1475 1507 422 Size: 32 520 32 469 32 3462 Fragments arranged by size: 3462 520 469 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 1852 3419 1852 Size: 1567 2980 ApaLI G'TGCA_C Cuts at: 3317 3317 Size: 4547 ApoI r'AATT_y Cuts at: 1263 1551 3900 4156 1263 Size: 288 2349 256 1654 Fragments arranged by size: 2349 1654 288 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4547 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4547 AvaI C'yCGr_G Cuts at: 1567 1567 Size: 4547 BamHI G'GATC_C Cuts at: 162 1572 162 Size: 1410 3137 BanI G'GyrC_C Cuts at: 1563 1977 2225 2260 1563 Size: 414 248 35 3850 Fragments arranged by size: 3850 414 248 35 BanII G_rGCy'C Cuts at: 1561 2591 1561 Size: 1030 3517 BbeI G_GCGC'C Cuts at: 1981 2229 1981 Size: 248 4299 BbsI GAAGACnn'nnnn_ Cuts at: 716 1066 716 Size: 350 4197 BbvCI CC'TCA_GC Cuts at: 200 200 Size: 4547 BciVI GTATCCnnnnn_n' Cuts at: 488 2445 3206 4547 488 Size: 1957 761 1341 488 Fragments arranged by size: 1957 1341 761 488 BclI T'GATC_A Cuts at: 2067 2067 Size: 4547 BglI GCCn_nnn'nGGC Cuts at: 196 1290 196 Size: 1094 3453 BglII A'GATC_T Cuts at: 2062 4099 2062 Size: 2037 2510 BlpI GC'TnA_GC Cuts at: 1659 1659 Size: 4547 Bme1580I G_kGCm'C Cuts at: 2172 2265 3321 2172 Size: 93 1056 3398 Fragments arranged by size: 3398 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1759 1840 2175 4319 1759 Size: 81 335 2144 1987 Fragments arranged by size: 2144 1987 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2914 2914 Size: 4547 Bpu10I CC'TnA_GC Cuts at: 200 200 Size: 4547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1723 2602 3094 3392 1723 Size: 879 492 298 2878 Fragments arranged by size: 2878 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4251 4251 Size: 4547 BsaAI yAC'GTr Cuts at: 654 1900 654 Size: 1246 3301 BsaBI GATnn'nnATC Cuts at: 6 691 1436 2061 4098 6 Size: 685 745 625 2037 455 Fragments arranged by size: 2037 745 685 625 455 BsaHI Gr'CG_yC Cuts at: 1978 2226 2928 4424 1978 Size: 248 702 1496 2101 Fragments arranged by size: 2101 1496 702 248 BsaWI w'CCGG_w Cuts at: 1736 2257 2935 3209 3356 3767 1736 Size: 521 678 274 147 411 2516 Fragments arranged by size: 2516 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 281 311 435 465 281 Size: 30 124 30 4363 Fragments arranged by size: 4363 124 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 136 1544 136 Size: 1408 3139 BseYI C'CCAG_C Cuts at: 1972 2730 3307 3803 1972 Size: 758 577 496 2716 Fragments arranged by size: 2716 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2089 2135 3343 104 Size: 1985 46 1208 1308 Fragments arranged by size: 1985 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1561 2339 2529 3321 1561 Size: 778 190 792 2787 Fragments arranged by size: 2787 792 778 190 BsmI GAATG_Cn' Cuts at: 514 514 Size: 4547 Bsp1286I G_dGCh'C Cuts at: 1561 2172 2265 2339 2529 2591 3321 1561 Size: 611 93 74 190 62 730 2787 Fragments arranged by size: 2787 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3767 3767 Size: 4547 BspHI T'CATG_A Cuts at: 1230 3723 1230 Size: 2493 2054 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1597 1811 2113 2494 1597 Size: 214 302 381 3650 Fragments arranged by size: 3650 381 302 214 BsrBI CCG'CTC Cuts at: 50 2839 2893 4377 4542 50 Size: 2789 54 1484 165 55 Fragments arranged by size: 2789 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2459 2459 Size: 4547 BsrFI r'CCGG_y Cuts at: 814 2545 2726 2935 814 Size: 1731 181 209 2426 Fragments arranged by size: 2426 1731 209 181 BssSI C'ACGA_G Cuts at: 2818 3176 2818 Size: 358 4189 BstAPI GCAn_nnn'nTGC Cuts at: 1924 1924 Size: 4547 BstBI TT'CG_AA Cuts at: 2908 2908 Size: 4547 BstXI CCAn_nnnn'nTGG Cuts at: 151 151 Size: 4547 BstYI r'GATC_y Cuts at: 1 162 1431 1572 2039 2062 2397 2643 3655 Size: 161 1269 141 467 23 335 246 1012 Cuts at: 3655 3741 3753 4099 1 Size: 86 12 346 449 Fragments arranged by size: 1269 1012 467 449 346 335 246 161 141 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4547 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 392 2497 2678 2841 392 Size: 2105 181 163 2098 Fragments arranged by size: 2105 2098 181 163 BtsI GCAGTG_nn' Cuts at: 1790 1790 Size: 4547 DraI TTT'AAA Cuts at: 1546 1546 Size: 4547 DrdI GACnn_nn'nnGTC Cuts at: 2253 3111 2253 Size: 858 3689 EaeI y'GGCC_r Cuts at: 2132 2306 2697 2724 4069 2132 Size: 174 391 27 1345 2610 Fragments arranged by size: 2610 1345 391 174 27 EagI C'GGCC_G Cuts at: 2132 2132 Size: 4547 EarI CTCTTCn'nnn_ Cuts at: 232 418 590 2570 2780 232 Size: 186 172 1980 210 1999 Fragments arranged by size: 1999 1980 210 186 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2755 3065 3211 4357 2755 Size: 310 146 1146 2945 Fragments arranged by size: 2945 1146 310 146 EcoICRI GAG'CTC Cuts at: 1559 1559 Size: 4547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1423 2372 2804 3551 3819 1423 Size: 949 432 747 268 2151 Fragments arranged by size: 2151 949 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 247 1686 247 Size: 1439 3108 EcoRI G'AATT_C Cuts at: 1551 1551 Size: 4547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 4547 FspI TGC'GCA Cuts at: 2328 2328 Size: 4547 HaeII r_GCGC'y Cuts at: 1981 2229 3251 4230 1981 Size: 248 1022 979 2298 Fragments arranged by size: 2298 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 268 300 439 471 764 796 2389 2419 2421 Size: 32 139 32 293 32 1593 30 2 Cuts at: 2421 2451 268 Size: 30 2364 Fragments arranged by size: 2364 1593 293 139 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1586 1586 Size: 4547 KasI G'GCGC_C Cuts at: 1977 2225 1977 Size: 248 4299 KpnI G_GTAC'C Cuts at: 1567 1567 Size: 4547 MfeI C'AATT_G Cuts at: 1103 1193 1103 Size: 90 4457 MluI A'CGCG_T Cuts at: 3980 3980 Size: 4547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 299 686 1254 1528 3218 3402 299 Size: 387 568 274 1690 184 1444 Fragments arranged by size: 1690 1444 568 387 274 184 MscI TGG'CCA Cuts at: 2308 2308 Size: 4547 MslI CAynn'nnrTG Cuts at: 149 875 1446 2536 2663 149 Size: 726 571 1090 127 2033 Fragments arranged by size: 2033 1090 726 571 127 MspA1I CmG'CkG Cuts at: 544 1658 1972 2332 3345 3590 544 Size: 1114 314 360 1013 245 1501 Fragments arranged by size: 1501 1114 1013 360 314 245 NaeI GCC'GGC Cuts at: 2728 2728 Size: 4547 NarI GG'CG_CC Cuts at: 1978 2226 1978 Size: 248 4299 NcoI C'CATG_G Cuts at: 107 107 Size: 4547 NgoMIV G'CCGG_C Cuts at: 2726 2726 Size: 4547 NspI r_CATG'y Cuts at: 1126 1282 1511 1600 2100 3007 1126 Size: 156 229 89 500 907 2666 Fragments arranged by size: 2666 907 500 229 156 89 PciI A'CATG_T Cuts at: 1278 1507 3003 1278 Size: 229 1496 2822 Fragments arranged by size: 2822 1496 229 PfoI T'CCnGG_A Cuts at: 136 136 Size: 4547 PmeI GTTT'AAAC Cuts at: 1546 1546 Size: 4547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3686 3718 3686 Size: 32 4515 PstI C_TGCA'G Cuts at: 258 627 1594 2279 258 Size: 369 967 685 2526 Fragments arranged by size: 2526 967 685 369 PvuI CG_AT'CG Cuts at: 104 104 Size: 4547 PvuII CAG'CTG Cuts at: 544 1972 2332 544 Size: 1428 360 2759 Fragments arranged by size: 2759 1428 360 RsrII CG'GwC_CG Cuts at: 2742 2742 Size: 4547 SacI G_AGCT'C Cuts at: 1561 1561 Size: 4547 SalI G'TCGA_C Cuts at: 1584 1584 Size: 4547 SapI GCTCTTCn'nnn_ Cuts at: 232 2570 2780 232 Size: 2338 210 1999 Fragments arranged by size: 2338 1999 210 SbfI CC_TGCA'GG Cuts at: 1594 1594 Size: 4547 ScaI AGT'ACT Cuts at: 4064 4064 Size: 4547 SfcI C'TryA_G Cuts at: 33 254 623 1590 2275 3268 3459 33 Size: 221 369 967 685 993 191 1121 Fragments arranged by size: 1121 993 967 685 369 221 191 SfoI GGC'GCC Cuts at: 1979 2227 1979 Size: 248 4299 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4547 SmaI CCC'GGG Cuts at: 1569 1569 Size: 4547 SmlI C'TyrA_G Cuts at: 1702 2617 3109 3371 1702 Size: 915 492 262 2878 Fragments arranged by size: 2878 915 492 262 SnaBI TAC'GTA Cuts at: 654 654 Size: 4547 SpeI A'CTAG_T Cuts at: 67 67 Size: 4547 SphI G_CATG'C Cuts at: 1600 2100 1600 Size: 500 4047 StuI AGG'CCT Cuts at: 755 755 Size: 4547 StyI C'CwwG_G Cuts at: 107 1681 2035 107 Size: 1574 354 2619 Fragments arranged by size: 2619 1574 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 537 2130 2263 2722 2931 537 Size: 1593 133 459 209 2153 Fragments arranged by size: 2153 1593 459 209 133 TatI w'GTAC_w Cuts at: 1210 4062 1210 Size: 2852 1695 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 281 313 900 932 1121 1153 4064 4096 281 Size: 32 587 32 189 32 2911 32 732 Fragments arranged by size: 2911 732 587 189 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2344 2344 Size: 4547 XbaI T'CTAG_A Cuts at: 1578 1578 Size: 4547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 2042 114 Size: 1928 2619 XmaI C'CCGG_G Cuts at: 1567 1567 Size: 4547 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BpmI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI