Restriction map for pF1KB8108
 (Circular) (Six-base) MAPSORT of: pF1KB8108.seq  Check: 1909  from: 1  to: 4547



pF1KB8108  4547 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:   1585    1585 
   Size:       4547
 
Acc65I G'GTAC_C

Cuts at:   1563    1563 
   Size:       4547
 
AclI AA'CG_TT

Cuts at:   4387    4387 
   Size:       4547
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1423    2372    2804    3551    3819    1423 
   Size:        949     432     747     268    2151

  Fragments arranged by size: 

                2151     949     747     432     268
 
AflIII A'CryG_T

Cuts at:   1278    1507    1899    3003    3980    1278 
   Size:        229     392    1104     977    1845

  Fragments arranged by size: 

                1845    1104     977     392     229
 
AgeI A'CCGG_T

Cuts at:   2935    2935 
   Size:       4547
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    422     454     974    1006    1475    1507     422 
   Size:         32     520      32     469      32    3462

  Fragments arranged by size: 

                3462     520     469      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1852    3419    1852 
   Size:       1567    2980
 
ApaLI G'TGCA_C

Cuts at:   3317    3317 
   Size:       4547
 
ApoI r'AATT_y

Cuts at:   1263    1551    3900    4156    1263 
   Size:        288    2349     256    1654

  Fragments arranged by size: 

                2349    1654     288     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4547
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4547
 
AvaI C'yCGr_G

Cuts at:   1567    1567 
   Size:       4547
 
BamHI G'GATC_C

Cuts at:    162    1572     162 
   Size:       1410    3137
 
BanI G'GyrC_C

Cuts at:   1563    1977    2225    2260    1563 
   Size:        414     248      35    3850

  Fragments arranged by size: 

                3850     414     248      35
 
BanII G_rGCy'C

Cuts at:   1561    2591    1561 
   Size:       1030    3517
 
BbeI G_GCGC'C

Cuts at:   1981    2229    1981 
   Size:        248    4299
 
BbsI GAAGACnn'nnnn_

Cuts at:    716    1066     716 
   Size:        350    4197
 
BbvCI CC'TCA_GC

Cuts at:    200     200 
   Size:       4547
 
BciVI GTATCCnnnnn_n'

Cuts at:    488    2445    3206    4547     488 
   Size:       1957     761    1341     488

  Fragments arranged by size: 

                1957    1341     761     488
 
BclI T'GATC_A

Cuts at:   2067    2067 
   Size:       4547
 
BglI GCCn_nnn'nGGC

Cuts at:    196    1290     196 
   Size:       1094    3453
 
BglII A'GATC_T

Cuts at:   2062    4099    2062 
   Size:       2037    2510
 
BlpI GC'TnA_GC

Cuts at:   1659    1659 
   Size:       4547
 
Bme1580I G_kGCm'C

Cuts at:   2172    2265    3321    2172 
   Size:         93    1056    3398

  Fragments arranged by size: 

                3398    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1759    1840    2175    4319    1759 
   Size:         81     335    2144    1987

  Fragments arranged by size: 

                2144    1987     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2914    2914 
   Size:       4547
 
Bpu10I CC'TnA_GC

Cuts at:    200     200 
   Size:       4547
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1723    2602    3094    3392    1723 
   Size:        879     492     298    2878

  Fragments arranged by size: 

                2878     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4251    4251 
   Size:       4547
 
BsaAI yAC'GTr

Cuts at:    654    1900     654 
   Size:       1246    3301
 
BsaBI GATnn'nnATC

Cuts at:      6     691    1436    2061    4098       6 
   Size:        685     745     625    2037     455

  Fragments arranged by size: 

                2037     745     685     625     455
 
BsaHI Gr'CG_yC

Cuts at:   1978    2226    2928    4424    1978 
   Size:        248     702    1496    2101

  Fragments arranged by size: 

                2101    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1736    2257    2935    3209    3356    3767    1736 
   Size:        521     678     274     147     411    2516

  Fragments arranged by size: 

                2516     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    281     311     435     465     281 
   Size:         30     124      30    4363

  Fragments arranged by size: 

                4363     124      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    136    1544     136 
   Size:       1408    3139
 
BseYI C'CCAG_C

Cuts at:   1972    2730    3307    3803    1972 
   Size:        758     577     496    2716

  Fragments arranged by size: 

                2716     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2089    2135    3343     104 
   Size:       1985      46    1208    1308

  Fragments arranged by size: 

                1985    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1561    2339    2529    3321    1561 
   Size:        778     190     792    2787

  Fragments arranged by size: 

                2787     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    514     514 
   Size:       4547
 
Bsp1286I G_dGCh'C

Cuts at:   1561    2172    2265    2339    2529    2591    3321    1561 
   Size:        611      93      74     190      62     730    2787

  Fragments arranged by size: 

                2787     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3767    3767 
   Size:       4547
 
BspHI T'CATG_A

Cuts at:   1230    3723    1230 
   Size:       2493    2054
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1597    1811    2113    2494    1597 
   Size:        214     302     381    3650

  Fragments arranged by size: 

                3650     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2839    2893    4377    4542      50 
   Size:       2789      54    1484     165      55

  Fragments arranged by size: 

                2789    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2459    2459 
   Size:       4547
 
BsrFI r'CCGG_y

Cuts at:    814    2545    2726    2935     814 
   Size:       1731     181     209    2426

  Fragments arranged by size: 

                2426    1731     209     181
 
BssSI C'ACGA_G

Cuts at:   2818    3176    2818 
   Size:        358    4189
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1924    1924 
   Size:       4547
 
BstBI TT'CG_AA

Cuts at:   2908    2908 
   Size:       4547
 
BstXI CCAn_nnnn'nTGG

Cuts at:    151     151 
   Size:       4547
 
BstYI r'GATC_y

Cuts at:      1     162    1431    1572    2039    2062    2397    2643    3655 
   Size:        161    1269     141     467      23     335     246    1012

Cuts at:   3655    3741    3753    4099       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1269    1012     467     449     346     335     246     161
                 141      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4547
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    392    2497    2678    2841     392 
   Size:       2105     181     163    2098

  Fragments arranged by size: 

                2105    2098     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1790    1790 
   Size:       4547
 
DraI TTT'AAA

Cuts at:   1546    1546 
   Size:       4547
 
DrdI GACnn_nn'nnGTC

Cuts at:   2253    3111    2253 
   Size:        858    3689
 
EaeI y'GGCC_r

Cuts at:   2132    2306    2697    2724    4069    2132 
   Size:        174     391      27    1345    2610

  Fragments arranged by size: 

                2610    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2132    2132 
   Size:       4547
 
EarI CTCTTCn'nnn_

Cuts at:    232     418     590    2570    2780     232 
   Size:        186     172    1980     210    1999

  Fragments arranged by size: 

                1999    1980     210     186     172
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2755    3065    3211    4357    2755 
   Size:        310     146    1146    2945

  Fragments arranged by size: 

                2945    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1559    1559 
   Size:       4547
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1423    2372    2804    3551    3819    1423 
   Size:        949     432     747     268    2151

  Fragments arranged by size: 

                2151     949     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    247    1686     247 
   Size:       1439    3108
 
EcoRI G'AATT_C

Cuts at:   1551    1551 
   Size:       4547
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2566    2566 
   Size:       4547
 
FspI TGC'GCA

Cuts at:   2328    2328 
   Size:       4547
 
HaeII r_GCGC'y

Cuts at:   1981    2229    3251    4230    1981 
   Size:        248    1022     979    2298

  Fragments arranged by size: 

                2298    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    268     300     439     471     764     796    2389    2419    2421 
   Size:         32     139      32     293      32    1593      30       2

Cuts at:   2421    2451     268 
   Size:         30    2364

  Fragments arranged by size: 

                2364    1593     293     139      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1586    1586 
   Size:       4547
 
KasI G'GCGC_C

Cuts at:   1977    2225    1977 
   Size:        248    4299
 
KpnI G_GTAC'C

Cuts at:   1567    1567 
   Size:       4547
 
MfeI C'AATT_G

Cuts at:   1103    1193    1103 
   Size:         90    4457
 
MluI A'CGCG_T

Cuts at:   3980    3980 
   Size:       4547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    299     686    1254    1528    3218    3402     299 
   Size:        387     568     274    1690     184    1444

  Fragments arranged by size: 

                1690    1444     568     387     274     184
 
MscI TGG'CCA

Cuts at:   2308    2308 
   Size:       4547
 
MslI CAynn'nnrTG

Cuts at:    149     875    1446    2536    2663     149 
   Size:        726     571    1090     127    2033

  Fragments arranged by size: 

                2033    1090     726     571     127
 
MspA1I CmG'CkG

Cuts at:    544    1658    1972    2332    3345    3590     544 
   Size:       1114     314     360    1013     245    1501

  Fragments arranged by size: 

                1501    1114    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2728    2728 
   Size:       4547
 
NarI GG'CG_CC

Cuts at:   1978    2226    1978 
   Size:        248    4299
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4547
 
NgoMIV G'CCGG_C

Cuts at:   2726    2726 
   Size:       4547
 
NspI r_CATG'y

Cuts at:   1126    1282    1511    1600    2100    3007    1126 
   Size:        156     229      89     500     907    2666

  Fragments arranged by size: 

                2666     907     500     229     156      89
 
PciI A'CATG_T

Cuts at:   1278    1507    3003    1278 
   Size:        229    1496    2822

  Fragments arranged by size: 

                2822    1496     229
 
PfoI T'CCnGG_A

Cuts at:    136     136 
   Size:       4547
 
PmeI GTTT'AAAC

Cuts at:   1546    1546 
   Size:       4547
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3686    3718    3686 
   Size:         32    4515
 
PstI C_TGCA'G

Cuts at:    258     627    1594    2279     258 
   Size:        369     967     685    2526

  Fragments arranged by size: 

                2526     967     685     369
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4547
 
PvuII CAG'CTG

Cuts at:    544    1972    2332     544 
   Size:       1428     360    2759

  Fragments arranged by size: 

                2759    1428     360
 
RsrII CG'GwC_CG

Cuts at:   2742    2742 
   Size:       4547
 
SacI G_AGCT'C

Cuts at:   1561    1561 
   Size:       4547
 
SalI G'TCGA_C

Cuts at:   1584    1584 
   Size:       4547
 
SapI GCTCTTCn'nnn_

Cuts at:    232    2570    2780     232 
   Size:       2338     210    1999

  Fragments arranged by size: 

                2338    1999     210
 
SbfI CC_TGCA'GG

Cuts at:   1594    1594 
   Size:       4547
 
ScaI AGT'ACT

Cuts at:   4064    4064 
   Size:       4547
 
SfcI C'TryA_G

Cuts at:     33     254     623    1590    2275    3268    3459      33 
   Size:        221     369     967     685     993     191    1121

  Fragments arranged by size: 

                1121     993     967     685     369     221     191
 
SfoI GGC'GCC

Cuts at:   1979    2227    1979 
   Size:        248    4299
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4547
 
SmaI CCC'GGG

Cuts at:   1569    1569 
   Size:       4547
 
SmlI C'TyrA_G

Cuts at:   1702    2617    3109    3371    1702 
   Size:        915     492     262    2878

  Fragments arranged by size: 

                2878     915     492     262
 
SnaBI TAC'GTA

Cuts at:    654     654 
   Size:       4547
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4547
 
SphI G_CATG'C

Cuts at:   1600    2100    1600 
   Size:        500    4047
 
StuI AGG'CCT

Cuts at:    755     755 
   Size:       4547
 
StyI C'CwwG_G

Cuts at:    107    1681    2035     107 
   Size:       1574     354    2619

  Fragments arranged by size: 

                2619    1574     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    537    2130    2263    2722    2931     537 
   Size:       1593     133     459     209    2153

  Fragments arranged by size: 

                2153    1593     459     209     133
 
TatI w'GTAC_w

Cuts at:   1210    4062    1210 
   Size:       2852    1695
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    281     313     900     932    1121    1153    4064    4096     281 
   Size:         32     587      32     189      32    2911      32     732

  Fragments arranged by size: 

                2911     732     587     189      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2344    2344 
   Size:       4547
 
XbaI T'CTAG_A

Cuts at:   1578    1578 
   Size:       4547
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    2042     114 
   Size:       1928    2619
 
XmaI C'CCGG_G

Cuts at:   1567    1567 
   Size:       4547
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI    BmgBI     BmtI     BpmI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI     SspI     SwaI
     XhoI     XmnI     ZraI
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