Restriction map for
pF1KB8031
(Circular) (Six-base) MAPSORT of: pF1KB8031.seq Check: 1995 from: 1 to: 4871
pF1KB8031 4871 bp
With 182 enzymes: SgfI *
May 21, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 1909 1909
Size: 4871
Acc65I G'GTAC_C
Cuts at: 1887 1887
Size: 4871
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1698 2696 3128 3875 4143 1064
Size: 634 998 432 747 268 1792
Fragments arranged by size:
1792 998 747 634 432 268
AflIII A'CryG_T
Cuts at: 1058 2223 3327 4304 1058
Size: 1165 1104 977 1625
Fragments arranged by size:
1625 1165 1104 977
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AlwNI CAG_nnn'CTG
Cuts at: 492 837 885 2176 3743 492
Size: 345 48 1291 1567 1620
Fragments arranged by size:
1620 1567 1291 345 48
ApaI G_GGCC'C
Cuts at: 513 513
Size: 4871
ApaLI G'TGCA_C
Cuts at: 482 3641 482
Size: 3159 1712
ApoI r'AATT_y
Cuts at: 1875 4224 4480 1875
Size: 2349 256 2266
Fragments arranged by size:
2349 2266 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 113 434 462 529 901 1154 1415 1891 113
Size: 321 28 67 372 253 261 476 3093
Fragments arranged by size:
3093 476 372 321 261 253 67 28
BamHI G'GATC_C
Cuts at: 1896 1896
Size: 4871
BanI G'GyrC_C
Cuts at: 444 525 924 973 1426 1521 1887 2301 2549
Size: 81 399 49 453 95 366 414 248
Cuts at: 2549 2584 444
Size: 35 2731
Fragments arranged by size:
2731 453 414 399 366 248 95 81
49 35
BanII G_rGCy'C
Cuts at: 304 513 539 1391 1470 1589 1709 1885 2915
Size: 209 26 852 79 119 120 176 1030
Cuts at: 2915 304
Size: 2260
Fragments arranged by size:
2260 1030 852 209 176 120 119 79
26
BbeI G_GCGC'C
Cuts at: 2305 2553 2305
Size: 248 4623
BbsI GAAGACnn'nnnn_
Cuts at: 801 801
Size: 4871
BbvCI CC'TCA_GC
Cuts at: 646 646
Size: 4871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 327 361 327
Size: 34 4837
BciVI GTATCCnnnnn_n'
Cuts at: 2769 3530 4871 2769
Size: 761 1341 2769
Fragments arranged by size:
2769 1341 761
BclI T'GATC_A
Cuts at: 1535 2391 1535
Size: 856 4015
BglI GCCn_nnn'nGGC
Cuts at: 569 569
Size: 4871
BglII A'GATC_T
Cuts at: 2386 4423 2386
Size: 2037 2834
BlpI GC'TnA_GC
Cuts at: 1225 1983 1225
Size: 758 4113
Bme1580I G_kGCm'C
Cuts at: 486 505 513 530 929 1526 2496 2589 3645
Size: 19 8 17 399 597 970 93 1056
Cuts at: 3645 486
Size: 1712
Fragments arranged by size:
1712 1056 970 597 399 93 19 17
8
BmgBI CAC'GTC
Cuts at: 1271 1271
Size: 4871
BmrI ACTGGGnnnn_n'
Cuts at: 507 508 1483 2083 2164 2499 4643 507
Size: 1 975 600 81 335 2144 735
Fragments arranged by size:
2144 975 735 600 335 81 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 304 3238 304
Size: 2934 1937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 799 940 1126 274
Size: 525 141 186 4019
Fragments arranged by size:
4019 525 186 141
Bpu10I CC'TnA_GC
Cuts at: 646 646
Size: 4871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 2047 2926 3418 3716 1069
Size: 978 879 492 298 2224
Fragments arranged by size:
2224 978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 775 1165 4575 775
Size: 390 3410 1071
Fragments arranged by size:
3410 1071 390
BsaAI yAC'GTr
Cuts at: 2224 2224
Size: 4871
BsaBI GATnn'nnATC
Cuts at: 6 2385 4422 6
Size: 2379 2037 455
Fragments arranged by size:
2379 2037 455
BsaHI Gr'CG_yC
Cuts at: 691 2302 2550 3252 4748 691
Size: 1611 248 702 1496 814
Fragments arranged by size:
1611 1496 814 702 248
BsaWI w'CCGG_w
Cuts at: 2060 2581 3259 3533 3680 4091 2060
Size: 521 678 274 147 411 2840
Fragments arranged by size:
2840 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1334 1364 1442 1472 1334
Size: 30 78 30 4733
Fragments arranged by size:
4733 78 30 30
BseYI C'CCAG_C
Cuts at: 147 825 1113 1164 1181 1287 2296 3054 3631
Size: 678 288 51 17 106 1009 758 577
Cuts at: 3631 4127 147
Size: 496 891
Fragments arranged by size:
1009 891 758 678 577 496 288 106
51 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 643 746 1747 321
Size: 322 103 1001 3445
Fragments arranged by size:
3445 1001 322 103
BsiEI CG_ry'CG
Cuts at: 104 2413 2459 3667 104
Size: 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 486 539 1391 1470 1589 1885 2663 2853 3645
Size: 53 852 79 119 296 778 190 792
Cuts at: 3645 486
Size: 1712
Fragments arranged by size:
1712 852 792 778 296 190 119 79
53
BsmI GAATG_Cn'
Cuts at: 1152 1152
Size: 4871
BsmBI CGTCTCn'nnnn_
Cuts at: 433 1072 1833 433
Size: 639 761 3471
Fragments arranged by size:
3471 761 639
Bsp1286I G_dGCh'C
Cuts at: 304 486 505 513 530 539 929 1391 1470
Size: 182 19 8 17 9 390 462 79
Cuts at: 1470 1526 1589 1709 1885 2496 2589 2663 2853
Size: 56 63 120 176 611 93 74 190
Cuts at: 2853 2915 3645 304
Size: 62 730 1530
Fragments arranged by size:
1530 730 611 462 390 190 182 176
120 93 79 74 63 62 56 19
17 9 8
BspEI T'CCGG_A
Cuts at: 4091 4091
Size: 4871
BspHI T'CATG_A
Cuts at: 1616 4047 1616
Size: 2431 2440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1169 1921 2135 2437 2818 1169
Size: 752 214 302 381 3222
Fragments arranged by size:
3222 752 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1651 3163 3217 4701 4866 50
Size: 1601 1512 54 1484 165 55
Fragments arranged by size:
1601 1512 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 848 1655 2783 848
Size: 807 1128 2936
Fragments arranged by size:
2936 1128 807
BsrFI r'CCGG_y
Cuts at: 447 2869 3050 3259 447
Size: 2422 181 209 2059
Fragments arranged by size:
2422 2059 209 181
BssSI C'ACGA_G
Cuts at: 3142 3500 3142
Size: 358 4513
BstAPI GCAn_nnn'nTGC
Cuts at: 973 2248 973
Size: 1275 3596
BstBI TT'CG_AA
Cuts at: 3232 3232
Size: 4871
BstXI CCAn_nnnn'nTGG
Cuts at: 755 777 1747 755
Size: 22 970 3879
Fragments arranged by size:
3879 970 22
BstYI r'GATC_y
Cuts at: 1 1896 2363 2386 2721 2967 3979 4065 4077
Size: 1895 467 23 335 246 1012 86 12
Cuts at: 4077 4423 1
Size: 346 449
Fragments arranged by size:
1895 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 733 740 1837 733
Size: 7 1097 3767
Fragments arranged by size:
3767 1097 7
BtgI C'CryG_G
Cuts at: 107 128 308 540 567 754 785 1257 1307
Size: 21 180 232 27 187 31 472 50
Cuts at: 1307 1360 1517 1746 107
Size: 53 157 229 3232
Fragments arranged by size:
3232 472 232 229 187 180 157 53
50 31 27 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 306 591 1360 1422 2821 3002 3165 306
Size: 285 769 62 1399 181 163 2012
Fragments arranged by size:
2012 1399 769 285 181 163 62
BtsI GCAGTG_nn'
Cuts at: 2114 2114
Size: 4871
ClaI AT'CG_AT
Cuts at: 1202 1202
Size: 4871
DraI TTT'AAA
Cuts at: 1870 1870
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 259 1269 2577 3435 259
Size: 1010 1308 858 1695
Fragments arranged by size:
1695 1308 1010 858
EaeI y'GGCC_r
Cuts at: 449 570 767 788 887 1034 1206 1241 1652
Size: 121 197 21 99 147 172 35 411
Cuts at: 1652 1749 2456 2630 3021 3048 4393 449
Size: 97 707 174 391 27 1345 927
Fragments arranged by size:
1345 927 707 411 391 197 174 172
147 121 99 97 35 27 21
EagI C'GGCC_G
Cuts at: 2456 2456
Size: 4871
EarI CTCTTCn'nnn_
Cuts at: 715 1837 2894 3104 715
Size: 1122 1057 210 2482
Fragments arranged by size:
2482 1122 1057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 389 1180 3079 3389 3535 4681 389
Size: 791 1899 310 146 1146 579
Fragments arranged by size:
1899 1146 791 579 310 146
EcoICRI GAG'CTC
Cuts at: 537 1389 1468 1587 1883 537
Size: 852 79 119 296 3525
Fragments arranged by size:
3525 852 296 119 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 799 940 1064 1126 1698 2696 3128 3875
Size: 525 141 124 62 572 998 432 747
Cuts at: 3875 4143 274
Size: 268 1002
Fragments arranged by size:
1002 998 747 572 525 432 268 141
124 62
EcoO109I rG'GnC_Cy
Cuts at: 207 509 744 882 1119 1158 1769 1811 2010
Size: 302 235 138 237 39 611 42 199
Cuts at: 2010 207
Size: 3068
Fragments arranged by size:
3068 611 302 237 235 199 138 42
39
EcoRI G'AATT_C
Cuts at: 1875 1875
Size: 4871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4871
FspI TGC'GCA
Cuts at: 2652 2652
Size: 4871
HaeII r_GCGC'y
Cuts at: 166 1307 2305 2553 3575 4554 166
Size: 1141 998 248 1022 979 483
Fragments arranged by size:
1141 1022 998 979 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 269 301 368 400 2713 2743 2745 2775 269
Size: 32 67 32 2313 30 2 30 2365
Fragments arranged by size:
2365 2313 67 32 32 30 30 2
HincII GTy'rAC
Cuts at: 351 695 1680 1910 351
Size: 344 985 230 3312
Fragments arranged by size:
3312 985 344 230
KasI G'GCGC_C
Cuts at: 2301 2549 2301
Size: 248 4623
KpnI G_GTAC'C
Cuts at: 1891 1891
Size: 4871
MluI A'CGCG_T
Cuts at: 4304 4304
Size: 4871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3542 3726 3542
Size: 184 4687
MscI TGG'CCA
Cuts at: 769 790 889 1036 1751 2632 769
Size: 21 99 147 715 881 3008
Fragments arranged by size:
3008 881 715 147 99 21
MslI CAynn'nnrTG
Cuts at: 753 2860 2987 753
Size: 2107 127 2637
Fragments arranged by size:
2637 2107 127
MspA1I CmG'CkG
Cuts at: 591 870 1982 2296 2656 3669 3914 591
Size: 279 1112 314 360 1013 245 1548
Fragments arranged by size:
1548 1112 1013 360 314 279 245
NaeI GCC'GGC
Cuts at: 449 3052 449
Size: 2603 2268
NarI GG'CG_CC
Cuts at: 2302 2550 2302
Size: 248 4623
NcoI C'CATG_G
Cuts at: 107 567 754 1517 107
Size: 460 187 763 3461
Fragments arranged by size:
3461 763 460 187
NgoMIV G'CCGG_C
Cuts at: 447 3050 447
Size: 2603 2268
NspI r_CATG'y
Cuts at: 631 1678 1924 2424 3331 631
Size: 1047 246 500 907 2171
Fragments arranged by size:
2171 1047 907 500 246
PasI CC'CwG_GG
Cuts at: 581 930 1548 581
Size: 349 618 3904
Fragments arranged by size:
3904 618 349
PciI A'CATG_T
Cuts at: 3327 3327
Size: 4871
PflMI CCAn_nnn'nTGG
Cuts at: 837 837
Size: 4871
PfoI T'CCnGG_A
Cuts at: 1391 1470 1391
Size: 79 4792
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4839
PpuMI rG'GwC_Cy
Cuts at: 744 882 1119 1158 744
Size: 138 237 39 4457
Fragments arranged by size:
4457 237 138 39
PspOMI G'GGCC_C
Cuts at: 509 509
Size: 4871
PstI C_TGCA'G
Cuts at: 881 969 1918 2603 881
Size: 88 949 685 3149
Fragments arranged by size:
3149 949 685 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 591 870 2296 2656 591
Size: 279 1426 360 2806
Fragments arranged by size:
2806 1426 360 279
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 539 1391 1470 1589 1885 539
Size: 852 79 119 296 3525
Fragments arranged by size:
3525 852 296 119 79
SalI G'TCGA_C
Cuts at: 1908 1908
Size: 4871
SanDI GG'GwC_CC
Cuts at: 1119 1119
Size: 4871
SapI GCTCTTCn'nnn_
Cuts at: 715 2894 3104 715
Size: 2179 210 2482
Fragments arranged by size:
2482 2179 210
SbfI CC_TGCA'GG
Cuts at: 969 1918 969
Size: 949 3922
ScaI AGT'ACT
Cuts at: 4388 4388
Size: 4871
SfcI C'TryA_G
Cuts at: 33 877 965 1233 1914 2599 3592 3783 33
Size: 844 88 268 681 685 993 191 1121
Fragments arranged by size:
1121 993 844 685 681 268 191 88
SfoI GGC'GCC
Cuts at: 2303 2551 2303
Size: 248 4623
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 115 436 531 1156 1417 1893 115
Size: 321 95 625 261 476 3093
Fragments arranged by size:
3093 625 476 321 261 95
SmlI C'TyrA_G
Cuts at: 1084 2026 2941 3433 3695 1084
Size: 942 915 492 262 2260
Fragments arranged by size:
2260 942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4871
SphI G_CATG'C
Cuts at: 1924 2424 1924
Size: 500 4371
StuI AGG'CCT
Cuts at: 226 738 1041 1249 226
Size: 512 303 208 3848
Fragments arranged by size:
3848 512 303 208
StyI C'CwwG_G
Cuts at: 107 257 567 754 854 1094 1517 2005 2359
Size: 150 310 187 100 240 423 488 354
Cuts at: 2359 107
Size: 2619
Fragments arranged by size:
2619 488 423 354 310 240 187 150
100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1450 2454 2587 3046 3255 1450
Size: 1004 133 459 209 3066
Fragments arranged by size:
3066 1004 459 209 133
TatI w'GTAC_w
Cuts at: 947 4386 947
Size: 3439 1432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1334 1366 1442 1474 4388 4420 1334
Size: 32 76 32 2914 32 1785
Fragments arranged by size:
2914 1785 76 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2668 2668
Size: 4871
XbaI T'CTAG_A
Cuts at: 1902 1902
Size: 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 327 565 798 1201 2366 211
Size: 116 238 233 403 1165 2716
Fragments arranged by size:
2716 1165 403 238 233 116
XmaI C'CCGG_G
Cuts at: 113 434 529 1154 1415 1891 113
Size: 321 95 625 261 476 3093
Fragments arranged by size:
3093 625 476 321 261 95
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BmtI BseRI BsiWI BsrGI
BssHII BstEII BstZ17I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI