Restriction map for
pF1KB8018
(Circular) (Six-base) MAPSORT of: pF1KB8018.seq Check: 774 from: 1 to: 4778
pF1KB8018 4778 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1607 1607
Size: 4778
AccI GT'mk_AC
Cuts at: 1816 1816
Size: 4778
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 4618 4618
Size: 4778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2603 3035 3782 4050 1129
Size: 1474 432 747 268 1857
Fragments arranged by size:
1857 1474 747 432 268
AflII C'TTAA_G
Cuts at: 973 973
Size: 4778
AflIII A'CryG_T
Cuts at: 810 2130 3234 4211 810
Size: 1320 1104 977 1377
Fragments arranged by size:
1377 1320 1104 977
AgeI A'CCGG_T
Cuts at: 1553 3166 1553
Size: 1613 3165
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1715 1747 1715
Size: 32 4746
AlwNI CAG_nnn'CTG
Cuts at: 228 249 709 2083 3650 228
Size: 21 460 1374 1567 1356
Fragments arranged by size:
1567 1374 1356 460 21
ApaLI G'TGCA_C
Cuts at: 317 3548 317
Size: 3231 1547
ApoI r'AATT_y
Cuts at: 834 1260 1782 4131 4387 834
Size: 426 522 2349 256 1225
Fragments arranged by size:
2349 1225 522 426 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 1175 1457 1798 1175
Size: 282 341 4155
Fragments arranged by size:
4155 341 282
BamHI G'GATC_C
Cuts at: 1803 1803
Size: 4778
BanI G'GyrC_C
Cuts at: 493 1794 2208 2456 2491 493
Size: 1301 414 248 35 2780
Fragments arranged by size:
2780 1301 414 248 35
BanII G_rGCy'C
Cuts at: 133 488 531 1540 1792 2822 133
Size: 355 43 1009 252 1030 2089
Fragments arranged by size:
2089 1030 1009 355 252 43
BbeI G_GCGC'C
Cuts at: 497 2212 2460 497
Size: 1715 248 2815
Fragments arranged by size:
2815 1715 248
BbvCI CC'TCA_GC
Cuts at: 1698 1698
Size: 4778
BciVI GTATCCnnnnn_n'
Cuts at: 1092 2676 3437 4778 1092
Size: 1584 761 1341 1092
Fragments arranged by size:
1584 1341 1092 761
BclI T'GATC_A
Cuts at: 2298 2298
Size: 4778
BglI GCCn_nnn'nGGC
Cuts at: 502 511 502
Size: 9 4769
BglII A'GATC_T
Cuts at: 850 1635 2293 4330 850
Size: 785 658 2037 1298
Fragments arranged by size:
2037 1298 785 658
BlpI GC'TnA_GC
Cuts at: 1890 1890
Size: 4778
Bme1580I G_kGCm'C
Cuts at: 321 2403 2496 3552 321
Size: 2082 93 1056 1547
Fragments arranged by size:
2082 1547 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1071 1990 2071 2406 4550 1071
Size: 919 81 335 2144 1299
Fragments arranged by size:
2144 1299 919 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1557 1559 3145 1557
Size: 2 1586 3190
Fragments arranged by size:
3190 1586 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 754 1545 271
Size: 483 791 3504
Fragments arranged by size:
3504 791 483
Bpu10I CC'TnA_GC
Cuts at: 1698 1698
Size: 4778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1954 2833 3325 3623 1954
Size: 879 492 298 3109
Fragments arranged by size:
3109 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4482 4482
Size: 4778
BsaAI yAC'GTr
Cuts at: 813 2131 813
Size: 1318 3460
BsaBI GATnn'nnATC
Cuts at: 6 2292 4329 6
Size: 2286 2037 455
Fragments arranged by size:
2286 2037 455
BsaHI Gr'CG_yC
Cuts at: 494 2209 2457 3159 4655 494
Size: 1715 248 702 1496 617
Fragments arranged by size:
1715 1496 702 617 248
BsaWI w'CCGG_w
Cuts at: 955 1553 1967 2488 3166 3440 3587 3998 955
Size: 598 414 521 678 274 147 411 1735
Fragments arranged by size:
1735 678 598 521 414 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 277 307 996 1026 1044 1074 1543 1573 1715
Size: 30 689 30 18 30 469 30 142
Cuts at: 1715 1745 277
Size: 30 3310
Fragments arranged by size:
3310 689 469 142 30 30 30 30
30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 1139 1530 1686 268
Size: 871 391 156 3360
Fragments arranged by size:
3360 871 391 156
BseYI C'CCAG_C
Cuts at: 258 2203 2961 3538 4034 258
Size: 1945 758 577 496 1002
Fragments arranged by size:
1945 1002 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1574 2320 2366 3574 104
Size: 1470 746 46 1208 1308
Fragments arranged by size:
1470 1308 1208 746 46
BsiHKAI G_wGCw'C
Cuts at: 321 488 1540 1792 2570 2760 3552 321
Size: 167 1052 252 778 190 792 1547
Fragments arranged by size:
1547 1052 792 778 252 190 167
BsmBI CGTCTCn'nnnn_
Cuts at: 1281 1281
Size: 4778
Bsp1286I G_dGCh'C
Cuts at: 133 321 488 531 1540 1792 2403 2496 2570
Size: 188 167 43 1009 252 611 93 74
Cuts at: 2570 2760 2822 3552 133
Size: 190 62 730 1359
Fragments arranged by size:
1359 1009 730 611 252 190 188 167
93 74 62 43
BspEI T'CCGG_A
Cuts at: 3998 3998
Size: 4778
BspHI T'CATG_A
Cuts at: 3954 3954
Size: 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1607 1828 2042 2344 2725 1607
Size: 221 214 302 381 3660
Fragments arranged by size:
3660 381 302 221 214
BsrBI CCG'CTC
Cuts at: 50 1512 1578 3070 3124 4608 4773 50
Size: 1462 66 1492 54 1484 165 55
Fragments arranged by size:
1492 1484 1462 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 1228 1301 2690 1228
Size: 73 1389 3316
Fragments arranged by size:
3316 1389 73
BsrFI r'CCGG_y
Cuts at: 1493 1553 2776 2957 3166 1493
Size: 60 1223 181 209 3105
Fragments arranged by size:
3105 1223 209 181 60
BsrGI T'GTAC_A
Cuts at: 782 1047 782
Size: 265 4513
BssSI C'ACGA_G
Cuts at: 3049 3407 3049
Size: 358 4420
BstAPI GCAn_nnn'nTGC
Cuts at: 228 2155 228
Size: 1927 2851
BstBI TT'CG_AA
Cuts at: 3139 3139
Size: 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 141 141
Size: 4778
BstYI r'GATC_y
Cuts at: 1 705 850 1134 1635 1803 2270 2293 2628
Size: 704 145 284 501 168 467 23 335
Cuts at: 2628 2874 3886 3972 3984 4330 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 704 501 467 449 346 335 284
246 168 145 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1241 1241
Size: 4778
BtgI C'CryG_G
Cuts at: 107 1393 107
Size: 1286 3492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2728 2909 3072 2728
Size: 181 163 4434
Fragments arranged by size:
4434 181 163
BtsI GCAGTG_nn'
Cuts at: 2021 2021
Size: 4778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 942 977 1382 1417 942
Size: 35 405 35 4303
Fragments arranged by size:
4303 405 35 35
DraI TTT'AAA
Cuts at: 668 1777 668
Size: 1109 3669
DrdI GACnn_nn'nnGTC
Cuts at: 419 1077 1460 2484 3342 419
Size: 658 383 1024 858 1855
Fragments arranged by size:
1855 1024 858 658 383
EaeI y'GGCC_r
Cuts at: 2363 2537 2928 2955 4300 2363
Size: 174 391 27 1345 2841
Fragments arranged by size:
2841 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2363 2363
Size: 4778
EarI CTCTTCn'nnn_
Cuts at: 1526 2801 3011 1526
Size: 1275 210 3293
Fragments arranged by size:
3293 1275 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1142 2986 3296 3442 4588 1142
Size: 1844 310 146 1146 1332
Fragments arranged by size:
1844 1332 1146 310 146
EcoICRI GAG'CTC
Cuts at: 486 1538 1790 486
Size: 1052 252 3474
Fragments arranged by size:
3474 1052 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 754 1129 1545 2603 3035 3782 4050 271
Size: 483 375 416 1058 432 747 268 999
Fragments arranged by size:
1058 999 747 483 432 416 375 268
EcoNI CCTnn'n_nnAGG
Cuts at: 767 767
Size: 4778
EcoO109I rG'GnC_Cy
Cuts at: 246 453 504 1917 246
Size: 207 51 1413 3107
Fragments arranged by size:
3107 1413 207 51
EcoRI G'AATT_C
Cuts at: 1260 1782 1260
Size: 522 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 443 636 2797 443
Size: 193 2161 2424
Fragments arranged by size:
2424 2161 193
FspI TGC'GCA
Cuts at: 2559 2559
Size: 4778
HaeII r_GCGC'y
Cuts at: 497 984 1652 2212 2460 3482 4461 497
Size: 487 668 560 248 1022 979 814
Fragments arranged by size:
1022 979 814 668 560 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 225 226 257 933 965 1044 1076 2620
Size: 31 1 31 676 32 79 32 1544
Cuts at: 2620 2650 2652 2682 194
Size: 30 2 30 2290
Fragments arranged by size:
2290 1544 676 79 32 32 31 31
30 30 2 1
HincII GTy'rAC
Cuts at: 1766 1817 1766
Size: 51 4727
KasI G'GCGC_C
Cuts at: 493 2208 2456 493
Size: 1715 248 2815
Fragments arranged by size:
2815 1715 248
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MluI A'CGCG_T
Cuts at: 4211 4211
Size: 4778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 275 3449 3633 143
Size: 132 3174 184 1288
Fragments arranged by size:
3174 1288 184 132
MscI TGG'CCA
Cuts at: 2539 2539
Size: 4778
MslI CAynn'nnrTG
Cuts at: 869 1360 2767 2894 869
Size: 491 1407 127 2753
Fragments arranged by size:
2753 1407 491 127
MspA1I CmG'CkG
Cuts at: 225 918 1742 1889 2203 2563 3576 3821 225
Size: 693 824 147 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 824 693 360 314 245 147
NaeI GCC'GGC
Cuts at: 2959 2959
Size: 4778
NarI GG'CG_CC
Cuts at: 494 2209 2457 494
Size: 1715 248 2815
Fragments arranged by size:
2815 1715 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4778
NgoMIV G'CCGG_C
Cuts at: 2957 2957
Size: 4778
NspI r_CATG'y
Cuts at: 731 783 1831 2331 3238 731
Size: 52 1048 500 907 2271
Fragments arranged by size:
2271 1048 907 500 52
PciI A'CATG_T
Cuts at: 3234 3234
Size: 4778
PflMI CCAn_nnn'nTGG
Cuts at: 1234 1384 1234
Size: 150 4628
PfoI T'CCnGG_A
Cuts at: 1130 1664 1130
Size: 534 4244
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PmlI CAC'GTG
Cuts at: 813 813
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1715 1747 3917 3949 1715
Size: 32 2170 32 2544
Fragments arranged by size:
2544 2170 32 32
PpuMI rG'GwC_Cy
Cuts at: 246 246
Size: 4778
PspXI vC'TCGA_Gb
Cuts at: 1457 1457
Size: 4778
PstI C_TGCA'G
Cuts at: 233 846 1825 2510 233
Size: 613 979 685 2501
Fragments arranged by size:
2501 979 685 613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 225 918 1742 2203 2563 225
Size: 693 824 461 360 2440
Fragments arranged by size:
2440 824 693 461 360
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 488 1540 1792 488
Size: 1052 252 3474
Fragments arranged by size:
3474 1052 252
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 2801 3011 2801
Size: 210 4568
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 4295 4295
Size: 4778
SfcI C'TryA_G
Cuts at: 33 229 602 842 1821 2506 3499 3690 33
Size: 196 373 240 979 685 993 191 1121
Fragments arranged by size:
1121 993 979 685 373 240 196 191
SfoI GGC'GCC
Cuts at: 495 2210 2458 495
Size: 1715 248 2815
Fragments arranged by size:
2815 1715 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 1177 1800 1177
Size: 623 4155
SmlI C'TyrA_G
Cuts at: 973 1457 1933 2848 3340 3602 973
Size: 484 476 915 492 262 2149
Fragments arranged by size:
2149 915 492 484 476 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4778
SphI G_CATG'C
Cuts at: 1831 2331 1831
Size: 500 4278
StuI AGG'CCT
Cuts at: 773 773
Size: 4778
StyI C'CwwG_G
Cuts at: 107 500 1912 2266 107
Size: 393 1412 354 2619
Fragments arranged by size:
2619 1412 393 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1588 2361 2494 2953 3162 1588
Size: 773 133 459 209 3204
Fragments arranged by size:
3204 773 459 209 133
TatI w'GTAC_w
Cuts at: 782 1047 4293 782
Size: 265 3246 1267
Fragments arranged by size:
3246 1267 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4295 4327 4295
Size: 32 4746
Tth111I GACn'n_nGTC
Cuts at: 877 2575 877
Size: 1698 3080
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 507 2273 141
Size: 366 1766 2646
Fragments arranged by size:
2646 1766 366
XhoI C'TCGA_G
Cuts at: 1457 1457
Size: 4778
XmaI C'CCGG_G
Cuts at: 1175 1798 1175
Size: 623 4155
XmnI GAAnn'nnTTC
Cuts at: 881 1264 881
Size: 383 4395
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI