Restriction map for
pF1KB8017
(Circular) (Six-base) MAPSORT of: pF1KB8017.seq Check: 6792 from: 1 to: 5054
pF1KB8017 5054 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 590 1958 590
Size: 1368 3686
AccI GT'mk_AC
Cuts at: 2092 2092
Size: 5054
Acc65I G'GTAC_C
Cuts at: 2070 2070
Size: 5054
AclI AA'CG_TT
Cuts at: 4894 4894
Size: 5054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 433 2879 3311 4058 4326 240
Size: 193 2446 432 747 268 968
Fragments arranged by size:
2446 968 747 432 268 193
AfeI AGC'GCT
Cuts at: 1624 1624
Size: 5054
AflIII A'CryG_T
Cuts at: 445 2406 3510 4487 445
Size: 1961 1104 977 1012
Fragments arranged by size:
1961 1104 1012 977
AgeI A'CCGG_T
Cuts at: 3442 3442
Size: 5054
AhdI GACnn_n'nnGTC
Cuts at: 643 643
Size: 5054
AleI CACnn'nnGTG
Cuts at: 484 601 1633 484
Size: 117 1032 3905
Fragments arranged by size:
3905 1032 117
AlwNI CAG_nnn'CTG
Cuts at: 228 558 1756 2359 3926 228
Size: 330 1198 603 1567 1356
Fragments arranged by size:
1567 1356 1198 603 330
ApaI G_GGCC'C
Cuts at: 342 1963 342
Size: 1621 3433
ApaLI G'TGCA_C
Cuts at: 3824 3824
Size: 5054
ApoI r'AATT_y
Cuts at: 750 2058 4407 4663 750
Size: 1308 2349 256 1141
Fragments arranged by size:
2349 1308 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
AvaI C'yCGr_G
Cuts at: 424 868 1836 1879 2074 424
Size: 444 968 43 195 3404
Fragments arranged by size:
3404 968 444 195 43
BamHI G'GATC_C
Cuts at: 720 2079 720
Size: 1359 3695
BanI G'GyrC_C
Cuts at: 682 1656 2070 2484 2732 2767 682
Size: 974 414 414 248 35 2969
Fragments arranged by size:
2969 974 414 414 248 35
BanII G_rGCy'C
Cuts at: 342 739 931 1036 1963 2068 3098 342
Size: 397 192 105 927 105 1030 2298
Fragments arranged by size:
2298 1030 927 397 192 105 105
BbeI G_GCGC'C
Cuts at: 2488 2736 2488
Size: 248 4806
BbsI GAAGACnn'nnnn_
Cuts at: 1579 1579
Size: 5054
BciVI GTATCCnnnnn_n'
Cuts at: 2952 3713 5054 2952
Size: 761 1341 2952
Fragments arranged by size:
2952 1341 761
BclI T'GATC_A
Cuts at: 1667 2574 1667
Size: 907 4147
BglI GCCn_nnn'nGGC
Cuts at: 674 674
Size: 5054
BglII A'GATC_T
Cuts at: 2569 4606 2569
Size: 2037 3017
BlpI GC'TnA_GC
Cuts at: 2166 2166
Size: 5054
Bme1580I G_kGCm'C
Cuts at: 342 515 812 1963 2679 2772 3828 342
Size: 173 297 1151 716 93 1056 1568
Fragments arranged by size:
1568 1151 1056 716 297 173 93
BmgBI CAC'GTC
Cuts at: 762 762
Size: 5054
BmrI ACTGGGnnnn_n'
Cuts at: 345 901 1039 1966 2266 2347 2682 4826 345
Size: 556 138 927 300 81 335 2144 573
Fragments arranged by size:
2144 927 573 556 335 300 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 5054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 205 841 1939 1990 156
Size: 49 636 1098 51 3220
Fragments arranged by size:
3220 1098 636 51 49
Bpu10I CC'TnA_GC
Cuts at: 925 925
Size: 5054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1237 1282 1585 2230 3109 3601 3899 1008
Size: 229 45 303 645 879 492 298 2163
Fragments arranged by size:
2163 879 645 492 303 298 229 45
BsaI GGTCTCn'nnnn_
Cuts at: 761 907 1016 1228 1478 1915 1990 4758 761
Size: 146 109 212 250 437 75 2768 1057
Fragments arranged by size:
2768 1057 437 250 212 146 109 75
BsaAI yAC'GTr
Cuts at: 446 2407 446
Size: 1961 3093
BsaBI GATnn'nnATC
Cuts at: 6 2568 4605 6
Size: 2562 2037 455
Fragments arranged by size:
2562 2037 455
BsaHI Gr'CG_yC
Cuts at: 2485 2733 3435 4931 2485
Size: 248 702 1496 2608
Fragments arranged by size:
2608 1496 702 248
BsaWI w'CCGG_w
Cuts at: 770 1220 2243 2764 3442 3716 3863 4274 770
Size: 450 1023 521 678 274 147 411 1550
Fragments arranged by size:
1550 1023 678 521 450 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 400 430 1447 1477 1507 400
Size: 30 1017 30 30 3947
Fragments arranged by size:
3947 1017 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1005 1246 1005
Size: 241 4813
BseYI C'CCAG_C
Cuts at: 403 612 903 946 1046 1140 2479 3237 3814
Size: 209 291 43 100 94 1339 758 577
Cuts at: 3814 4310 403
Size: 496 1147
Fragments arranged by size:
1339 1147 758 577 496 291 209 100
94 43
BsiEI CG_ry'CG
Cuts at: 104 1114 1677 2596 2642 3850 104
Size: 1010 563 919 46 1208 1308
Fragments arranged by size:
1308 1208 1010 919 563 46
BsiHKAI G_wGCw'C
Cuts at: 370 494 692 931 1298 1316 2068 2846 3036
Size: 124 198 239 367 18 752 778 190
Cuts at: 3036 3828 370
Size: 792 1596
Fragments arranged by size:
1596 792 778 752 367 239 198 190
124 18
BsmBI CGTCTCn'nnnn_
Cuts at: 1561 1561
Size: 5054
Bsp1286I G_dGCh'C
Cuts at: 342 370 494 515 692 739 812 931 1036
Size: 28 124 21 177 47 73 119 105
Cuts at: 1036 1298 1316 1963 2068 2679 2772 2846 3036
Size: 262 18 647 105 611 93 74 190
Cuts at: 3036 3098 3828 342
Size: 62 730 1568
Fragments arranged by size:
1568 730 647 611 262 190 177 124
119 105 105 93 74 73 62 47
28 21 18
BspEI T'CCGG_A
Cuts at: 4274 4274
Size: 5054
BspHI T'CATG_A
Cuts at: 4230 4230
Size: 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 836 1772 1958 2104 2318 2620 3001 590
Size: 246 936 186 146 214 302 381 2643
Fragments arranged by size:
2643 936 381 302 246 214 186 146
BsrBI CCG'CTC
Cuts at: 50 2018 3346 3400 4884 5049 50
Size: 1968 1328 54 1484 165 55
Fragments arranged by size:
1968 1484 1328 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2966 2966
Size: 5054
BsrFI r'CCGG_y
Cuts at: 1091 1942 2003 3052 3233 3442 1091
Size: 851 61 1049 181 209 2703
Fragments arranged by size:
2703 1049 851 209 181 61
BssSI C'ACGA_G
Cuts at: 3325 3683 3325
Size: 358 4696
BstAPI GCAn_nnn'nTGC
Cuts at: 558 1151 2431 558
Size: 593 1280 3181
Fragments arranged by size:
3181 1280 593
BstBI TT'CG_AA
Cuts at: 3415 3415
Size: 5054
BstEII G'GTnAC_C
Cuts at: 534 534
Size: 5054
BstXI CCAn_nnnn'nTGG
Cuts at: 390 390
Size: 5054
BstYI r'GATC_y
Cuts at: 1 200 554 720 773 2079 2546 2569 2904
Size: 199 354 166 53 1306 467 23 335
Cuts at: 2904 3150 4162 4248 4260 4606 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1306 1012 467 449 354 346 335 246
199 166 86 53 23 12
Bsu36I CC'TnA_GG
Cuts at: 1583 2036 1583
Size: 453 4601
BtgI C'CryG_G
Cuts at: 360 897 1128 1405 1631 1775 360
Size: 537 231 277 226 144 3639
Fragments arranged by size:
3639 537 277 231 226 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1287 1422 3004 3185 3348 1287
Size: 135 1582 181 163 2993
Fragments arranged by size:
2993 1582 181 163 135
BtsI GCAGTG_nn'
Cuts at: 279 513 2297 279
Size: 234 1784 3036
Fragments arranged by size:
3036 1784 234
DraI TTT'AAA
Cuts at: 2053 2053
Size: 5054
DraIII CAC_nnn'GTG
Cuts at: 1183 1183
Size: 5054
DrdI GACnn_nn'nnGTC
Cuts at: 2760 3618 2760
Size: 858 4196
EaeI y'GGCC_r
Cuts at: 192 210 1093 1111 1158 1674 1940 1944 2639
Size: 18 883 18 47 516 266 4 695
Cuts at: 2639 2813 3204 3231 4576 192
Size: 174 391 27 1345 670
Fragments arranged by size:
1345 883 695 670 516 391 266 174
47 27 18 18 4
EagI C'GGCC_G
Cuts at: 1111 1674 2639 1111
Size: 563 965 3526
Fragments arranged by size:
3526 965 563
EarI CTCTTCn'nnn_
Cuts at: 322 1879 3077 3287 322
Size: 1557 1198 210 2089
Fragments arranged by size:
2089 1557 1198 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3262 3572 3718 4864 3262
Size: 310 146 1146 3452
Fragments arranged by size:
3452 1146 310 146
EcoICRI GAG'CTC
Cuts at: 929 2066 929
Size: 1137 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 205 240 433 841 1939 1990 2879 3311
Size: 49 35 193 408 1098 51 889 432
Cuts at: 3311 4058 4326 156
Size: 747 268 884
Fragments arranged by size:
1098 889 884 747 432 408 268 193
51 49 35
EcoNI CCTnn'n_nnAGG
Cuts at: 1255 1255
Size: 5054
EcoO109I rG'GnC_Cy
Cuts at: 225 452 907 1056 1587 2040 2193 225
Size: 227 455 149 531 453 153 3086
Fragments arranged by size:
3086 531 455 453 227 153 149
EcoRI G'AATT_C
Cuts at: 750 2058 750
Size: 1308 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1170 3073 1170
Size: 1903 3151
FseI GG_CCGG'CC
Cuts at: 1946 1946
Size: 5054
FspI TGC'GCA
Cuts at: 2835 2835
Size: 5054
HaeII r_GCGC'y
Cuts at: 1022 1626 2488 2736 3758 4737 1022
Size: 604 862 248 1022 979 1339
Fragments arranged by size:
1339 1022 979 862 604 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 629 661 758 790 1426 1458 1477 1509 1690
Size: 32 97 32 636 32 19 32 181
Cuts at: 1690 1722 2896 2926 2928 2958 629
Size: 32 1174 30 2 30 2725
Fragments arranged by size:
2725 1174 636 181 97 32 32 32
32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 116 2093 116
Size: 1977 3077
KasI G'GCGC_C
Cuts at: 2484 2732 2484
Size: 248 4806
KpnI G_GTAC'C
Cuts at: 2074 2074
Size: 5054
MluI A'CGCG_T
Cuts at: 4487 4487
Size: 5054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 744 1097 3725 3909 744
Size: 353 2628 184 1889
Fragments arranged by size:
2628 1889 353 184
MscI TGG'CCA
Cuts at: 212 2815 212
Size: 2603 2451
MslI CAynn'nnrTG
Cuts at: 484 601 1309 1633 1855 3043 3170 484
Size: 117 708 324 222 1188 127 2368
Fragments arranged by size:
2368 1188 708 324 222 127 117
MspA1I CmG'CkG
Cuts at: 861 950 1968 1977 2165 2479 2839 3852 4097
Size: 89 1018 9 188 314 360 1013 245
Cuts at: 4097 861
Size: 1818
Fragments arranged by size:
1818 1018 1013 360 314 245 188 89
9
NaeI GCC'GGC
Cuts at: 1093 1944 3235 1093
Size: 851 1291 2912
Fragments arranged by size:
2912 1291 851
NarI GG'CG_CC
Cuts at: 2485 2733 2485
Size: 248 4806
NcoI C'CATG_G
Cuts at: 360 1405 1775 360
Size: 1045 370 3639
Fragments arranged by size:
3639 1045 370
NgoMIV G'CCGG_C
Cuts at: 1091 1942 3233 1091
Size: 851 1291 2912
Fragments arranged by size:
2912 1291 851
NspI r_CATG'y
Cuts at: 1935 2107 2607 3514 1935
Size: 172 500 907 3475
Fragments arranged by size:
3475 907 500 172
PciI A'CATG_T
Cuts at: 3510 3510
Size: 5054
PflMI CCAn_nnn'nTGG
Cuts at: 531 717 903 531
Size: 186 186 4682
Fragments arranged by size:
4682 186 186
PfoI T'CCnGG_A
Cuts at: 1805 1805
Size: 5054
PmeI GTTT'AAAC
Cuts at: 2053 2053
Size: 5054
PmlI CAC'GTG
Cuts at: 446 446
Size: 5054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 5022
PpuMI rG'GwC_Cy
Cuts at: 452 1056 2040 452
Size: 604 984 3466
Fragments arranged by size:
3466 984 604
PshAI GACnn'nnGTC
Cuts at: 541 1061 541
Size: 520 4534
PspOMI G'GGCC_C
Cuts at: 338 1959 338
Size: 1621 3433
PspXI vC'TCGA_Gb
Cuts at: 868 868
Size: 5054
PstI C_TGCA'G
Cuts at: 274 1000 2101 2786 274
Size: 726 1101 685 2542
Fragments arranged by size:
2542 1101 726 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5054
PvuII CAG'CTG
Cuts at: 861 1968 1977 2479 2839 861
Size: 1107 9 502 360 3076
Fragments arranged by size:
3076 1107 502 360 9
RsrII CG'GwC_CG
Cuts at: 3249 3249
Size: 5054
SacI G_AGCT'C
Cuts at: 931 2068 931
Size: 1137 3917
SalI G'TCGA_C
Cuts at: 2091 2091
Size: 5054
SapI GCTCTTCn'nnn_
Cuts at: 322 3077 3287 322
Size: 2755 210 2089
Fragments arranged by size:
2755 2089 210
SbfI CC_TGCA'GG
Cuts at: 2101 2101
Size: 5054
ScaI AGT'ACT
Cuts at: 295 4571 295
Size: 4276 778
SfcI C'TryA_G
Cuts at: 33 270 996 2097 2782 3775 3966 33
Size: 237 726 1101 685 993 191 1121
Fragments arranged by size:
1121 1101 993 726 685 237 191
SfoI GGC'GCC
Cuts at: 2486 2734 2486
Size: 248 4806
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
SmaI CCC'GGG
Cuts at: 2076 2076
Size: 5054
SmlI C'TyrA_G
Cuts at: 868 987 1252 1297 1600 2209 3124 3616 3878
Size: 119 265 45 303 609 915 492 262
Cuts at: 3878 868
Size: 2044
Fragments arranged by size:
2044 915 609 492 303 265 262 119
45
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5054
SphI G_CATG'C
Cuts at: 1935 2107 2607 1935
Size: 172 500 4382
Fragments arranged by size:
4382 500 172
StyI C'CwwG_G
Cuts at: 360 383 1405 1430 1775 2188 2542 360
Size: 23 1022 25 345 413 354 2872
Fragments arranged by size:
2872 1022 413 354 345 25 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 353 2637 2770 3229 3438 353
Size: 2284 133 459 209 1969
Fragments arranged by size:
2284 1969 459 209 133
TatI w'GTAC_w
Cuts at: 293 503 1349 4569 293
Size: 210 846 3220 778
Fragments arranged by size:
3220 846 778 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 686 718 4571 4603 686
Size: 32 3853 32 1137
Fragments arranged by size:
3853 1137 32 32
Tth111I GACn'n_nGTC
Cuts at: 2851 2851
Size: 5054
XbaI T'CTAG_A
Cuts at: 2085 2085
Size: 5054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2549 2549
Size: 5054
XhoI C'TCGA_G
Cuts at: 868 868
Size: 5054
XmaI C'CCGG_G
Cuts at: 2074 2074
Size: 5054
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsgI BsiWI BsmI BsrGI BssHII
BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI