Restriction map for
pF1KB8006
(Circular) (Six-base) MAPSORT of: pF1KB8006.seq Check: 4642 from: 1 to: 5432
pF1KB8006 5432 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 423 1362 423
Size: 939 4493
AccI GT'mk_AC
Cuts at: 1931 2470 1931
Size: 539 4893
Acc65I G'GTAC_C
Cuts at: 2448 2448
Size: 5432
AclI AA'CG_TT
Cuts at: 5272 5272
Size: 5432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 2134 3257 3689 4436 4704 1883
Size: 251 1123 432 747 268 2611
Fragments arranged by size:
2611 1123 747 432 268 251
AfeI AGC'GCT
Cuts at: 194 194
Size: 5432
AflII C'TTAA_G
Cuts at: 442 451 442
Size: 9 5423
AflIII A'CryG_T
Cuts at: 2015 2166 2784 3888 4865 2015
Size: 151 618 1104 977 2582
Fragments arranged by size:
2582 1104 977 618 151
AgeI A'CCGG_T
Cuts at: 3820 3820
Size: 5432
AhdI GACnn_n'nnGTC
Cuts at: 927 927
Size: 5432
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 431 2285 431
Size: 1854 3578
AlwNI CAG_nnn'CTG
Cuts at: 1357 2157 2737 4304 1357
Size: 800 580 1567 2485
Fragments arranged by size:
2485 1567 800 580
ApaI G_GGCC'C
Cuts at: 149 1319 1365 149
Size: 1170 46 4216
Fragments arranged by size:
4216 1170 46
ApaLI G'TGCA_C
Cuts at: 2412 4202 2412
Size: 1790 3642
ApoI r'AATT_y
Cuts at: 813 1423 1477 2436 4785 5041 813
Size: 610 54 959 2349 256 1204
Fragments arranged by size:
2349 1204 959 610 256 54
AseI AT'TA_AT
Cuts at: 20 20
Size: 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
AvaI C'yCGr_G
Cuts at: 1079 1311 1417 1814 1891 2452 1079
Size: 232 106 397 77 561 4059
Fragments arranged by size:
4059 561 397 232 106 77
BamHI G'GATC_C
Cuts at: 340 2457 340
Size: 2117 3315
BanI G'GyrC_C
Cuts at: 1735 2448 2862 3110 3145 1735
Size: 713 414 248 35 4022
Fragments arranged by size:
4022 713 414 248 35
BanII G_rGCy'C
Cuts at: 127 149 180 1319 1365 2408 2446 3476 127
Size: 22 31 1139 46 1043 38 1030 2083
Fragments arranged by size:
2083 1139 1043 1030 46 38 31 22
BbeI G_GCGC'C
Cuts at: 2866 3114 2866
Size: 248 5184
BbvCI CC'TCA_GC
Cuts at: 296 1346 2302 2336 296
Size: 1050 956 34 3392
Fragments arranged by size:
3392 1050 956 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 308 832 866 1492 1526 274
Size: 34 524 34 626 34 4180
Fragments arranged by size:
4180 626 524 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3330 4091 5432 3330
Size: 761 1341 3330
Fragments arranged by size:
3330 1341 761
BclI T'GATC_A
Cuts at: 2952 2952
Size: 5432
BglI GCCn_nnn'nGGC
Cuts at: 1360 1360
Size: 5432
BglII A'GATC_T
Cuts at: 2947 4984 2947
Size: 2037 3395
BlpI GC'TnA_GC
Cuts at: 1493 2544 1493
Size: 1051 4381
Bme1580I G_kGCm'C
Cuts at: 149 879 1319 1365 2416 3057 3150 4206 149
Size: 730 440 46 1051 641 93 1056 1375
Fragments arranged by size:
1375 1056 1051 730 641 440 93 46
BmgBI CAC'GTC
Cuts at: 537 582 2018 2424 537
Size: 45 1436 406 3545
Fragments arranged by size:
3545 1436 406 45
BmrI ACTGGGnnnn_n'
Cuts at: 2187 2644 2725 3060 5204 2187
Size: 457 81 335 2144 2415
Fragments arranged by size:
2415 2144 457 335 81
BmtI G_CTAG'C
Cuts at: 2335 2335
Size: 5432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 144 146 194 1311 3799 144
Size: 2 48 1117 2488 1777
Fragments arranged by size:
2488 1777 1117 48 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1291 1459 1070
Size: 221 168 5043
Fragments arranged by size:
5043 221 168
Bpu10I CC'TnA_GC
Cuts at: 296 562 1252 1346 2302 2336 296
Size: 266 690 94 956 34 3392
Fragments arranged by size:
3392 956 690 266 94 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2608 3487 3979 4277 1663
Size: 945 879 492 298 2818
Fragments arranged by size:
2818 945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5136 5136
Size: 5432
BsaAI yAC'GTr
Cuts at: 2785 2785
Size: 5432
BsaBI GATnn'nnATC
Cuts at: 6 2946 4983 6
Size: 2940 2037 455
Fragments arranged by size:
2940 2037 455
BsaHI Gr'CG_yC
Cuts at: 2383 2863 3111 3813 5309 2383
Size: 480 248 702 1496 2506
Fragments arranged by size:
2506 1496 702 480 248
BsaWI w'CCGG_w
Cuts at: 422 2621 3142 3820 4094 4241 4652 422
Size: 2199 521 678 274 147 411 1202
Fragments arranged by size:
2199 1202 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 176 597 627 1043 1073 146
Size: 30 421 30 416 30 4505
Fragments arranged by size:
4505 421 416 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 673 1133 2332 2362 352
Size: 321 460 1199 30 3422
Fragments arranged by size:
3422 1199 460 321 30
BseYI C'CCAG_C
Cuts at: 1951 2390 2857 3615 4192 4688 1951
Size: 439 467 758 577 496 2695
Fragments arranged by size:
2695 758 577 496 467 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 970 1639 2143 2278 263
Size: 707 669 504 135 3417
Fragments arranged by size:
3417 707 669 504 135
BsiEI CG_ry'CG
Cuts at: 104 2974 3020 4228 104
Size: 2870 46 1208 1308
Fragments arranged by size:
2870 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 180 704 983 2416 2446 3224 3414 4206 180
Size: 524 279 1433 30 778 190 792 1406
Fragments arranged by size:
1433 1406 792 778 524 279 190 30
Bsp1286I G_dGCh'C
Cuts at: 127 149 180 704 879 983 1319 1365 2408
Size: 22 31 524 175 104 336 46 1043
Cuts at: 2408 2416 2446 3057 3150 3224 3414 3476 4206
Size: 8 30 611 93 74 190 62 730
Cuts at: 4206 127
Size: 1353
Fragments arranged by size:
1353 1043 730 611 524 336 190 175
104 93 74 62 46 31 30 22
8
BspEI T'CCGG_A
Cuts at: 422 4652 422
Size: 4230 1202
BspHI T'CATG_A
Cuts at: 1031 4608 1031
Size: 3577 1855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 1362 2300 2482 2696 2998 3379 423
Size: 939 938 182 214 302 381 2476
Fragments arranged by size:
2476 939 938 381 302 214 182
BsrBI CCG'CTC
Cuts at: 50 135 1497 3724 3778 5262 5427 50
Size: 85 1362 2227 54 1484 165 55
Fragments arranged by size:
2227 1484 1362 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 3344 3344
Size: 5432
BsrFI r'CCGG_y
Cuts at: 3430 3611 3820 3430
Size: 181 209 5042
Fragments arranged by size:
5042 209 181
BsrGI T'GTAC_A
Cuts at: 515 515
Size: 5432
BssSI C'ACGA_G
Cuts at: 1657 1981 3703 4061 1657
Size: 324 1722 358 3028
Fragments arranged by size:
3028 1722 358 324
BstAPI GCAn_nnn'nTGC
Cuts at: 2091 2809 2091
Size: 718 4714
BstBI TT'CG_AA
Cuts at: 1428 3793 1428
Size: 2365 3067
BstEII G'GTnAC_C
Cuts at: 1086 1743 1086
Size: 657 4775
BstXI CCAn_nnnn'nTGG
Cuts at: 1048 1048
Size: 5432
BstYI r'GATC_y
Cuts at: 1 340 1768 1973 2144 2457 2924 2947 3282
Size: 339 1428 205 171 313 467 23 335
Cuts at: 3282 3528 4540 4626 4638 4984 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1428 1012 467 449 346 339 335 313
246 205 171 86 23 12
BtgI C'CryG_G
Cuts at: 107 2001 2417 107
Size: 1894 416 3122
Fragments arranged by size:
3122 1894 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3382 3563 3726 3382
Size: 181 163 5088
Fragments arranged by size:
5088 181 163
BtsI GCAGTG_nn'
Cuts at: 704 1215 2675 704
Size: 511 1460 3461
Fragments arranged by size:
3461 1460 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 972 1007 972
Size: 35 5397
DraI TTT'AAA
Cuts at: 2431 2431
Size: 5432
DraIII CAC_nnn'GTG
Cuts at: 2091 2091
Size: 5432
DrdI GACnn_nn'nnGTC
Cuts at: 1927 3138 3996 1927
Size: 1211 858 3363
Fragments arranged by size:
3363 1211 858
EaeI y'GGCC_r
Cuts at: 384 993 2202 3017 3191 3582 3609 4954 384
Size: 609 1209 815 174 391 27 1345 862
Fragments arranged by size:
1345 1209 862 815 609 391 174 27
EagI C'GGCC_G
Cuts at: 3017 3017
Size: 5432
EarI CTCTTCn'nnn_
Cuts at: 332 797 1859 2050 2320 3455 3665 332
Size: 465 1062 191 270 1135 210 2099
Fragments arranged by size:
2099 1135 1062 465 270 210 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 559 926 1549 1675 2376 3640 3950 4096 5242
Size: 367 623 126 701 1264 310 146 1146
Cuts at: 5242 559
Size: 749
Fragments arranged by size:
1264 1146 749 701 623 367 310 146
126
EcoICRI GAG'CTC
Cuts at: 178 2444 178
Size: 2266 3166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1291 1459 1883 2134 3257 3689 4436 4704
Size: 221 168 424 251 1123 432 747 268
Cuts at: 4704 1070
Size: 1798
Fragments arranged by size:
1798 1123 747 432 424 268 251 221
168
EcoNI CCTnn'n_nnAGG
Cuts at: 1095 1369 1095
Size: 274 5158
EcoO109I rG'GnC_Cy
Cuts at: 300 1315 1316 1361 1362 2571 300
Size: 1015 1 45 1 1209 3161
Fragments arranged by size:
3161 1209 1015 45 1 1
EcoRI G'AATT_C
Cuts at: 2436 2436
Size: 5432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 886 1745 3451 886
Size: 859 1706 2867
Fragments arranged by size:
2867 1706 859
FspI TGC'GCA
Cuts at: 2194 3213 2194
Size: 1019 4413
HaeII r_GCGC'y
Cuts at: 196 2866 3114 4136 5115 196
Size: 2670 248 1022 979 513
Fragments arranged by size:
2670 1022 979 513 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 265 279 297 913 945 3274 3304 3306
Size: 18 14 18 616 32 2329 30 2
Cuts at: 3306 3336 247
Size: 30 2343
Fragments arranged by size:
2343 2329 616 32 30 30 18 18
14 2
HincII GTy'rAC
Cuts at: 2471 2471
Size: 5432
KasI G'GCGC_C
Cuts at: 2862 3110 2862
Size: 248 5184
KpnI G_GTAC'C
Cuts at: 2452 2452
Size: 5432
MluI A'CGCG_T
Cuts at: 4865 4865
Size: 5432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2100 4103 4287 2100
Size: 2003 184 3245
Fragments arranged by size:
3245 2003 184
MscI TGG'CCA
Cuts at: 386 3193 386
Size: 2807 2625
MslI CAynn'nnrTG
Cuts at: 2165 3421 3548 2165
Size: 1256 127 4049
Fragments arranged by size:
4049 1256 127
MspA1I CmG'CkG
Cuts at: 1014 2154 2543 2857 3217 4230 4475 1014
Size: 1140 389 314 360 1013 245 1971
Fragments arranged by size:
1971 1140 1013 389 360 314 245
NaeI GCC'GGC
Cuts at: 3613 3613
Size: 5432
NarI GG'CG_CC
Cuts at: 2863 3111 2863
Size: 248 5184
NcoI C'CATG_G
Cuts at: 107 2001 107
Size: 1894 3538
NgoMIV G'CCGG_C
Cuts at: 3611 3611
Size: 5432
NheI G'CTAG_C
Cuts at: 2331 2331
Size: 5432
NspI r_CATG'y
Cuts at: 589 1668 2170 2199 2485 2985 3892 589
Size: 1079 502 29 286 500 907 2129
Fragments arranged by size:
2129 1079 907 502 500 286 29
PasI CC'CwG_GG
Cuts at: 1357 1365 1357
Size: 8 5424
PciI A'CATG_T
Cuts at: 2166 3888 2166
Size: 1722 3710
PflMI CCAn_nnn'nTGG
Cuts at: 2007 2007
Size: 5432
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 4571
Size: 32 5400
PpuMI rG'GwC_Cy
Cuts at: 300 300
Size: 5432
PsiI TTA'TAA
Cuts at: 1172 1172
Size: 5432
PspOMI G'GGCC_C
Cuts at: 145 1315 1361 145
Size: 1170 46 4216
Fragments arranged by size:
4216 1170 46
PspXI vC'TCGA_Gb
Cuts at: 1891 1891
Size: 5432
PstI C_TGCA'G
Cuts at: 493 1266 2264 2479 3164 493
Size: 773 998 215 685 2761
Fragments arranged by size:
2761 998 773 685 215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5432
PvuII CAG'CTG
Cuts at: 2154 2857 3217 2154
Size: 703 360 4369
Fragments arranged by size:
4369 703 360
RsrII CG'GwC_CG
Cuts at: 420 3627 420
Size: 3207 2225
SacI G_AGCT'C
Cuts at: 180 2446 180
Size: 2266 3166
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5432
SapI GCTCTTCn'nnn_
Cuts at: 3455 3665 3455
Size: 210 5222
SbfI CC_TGCA'GG
Cuts at: 2479 2479
Size: 5432
ScaI AGT'ACT
Cuts at: 4949 4949
Size: 5432
SexAI A'CCwGG_T
Cuts at: 1090 1090
Size: 5432
SfcI C'TryA_G
Cuts at: 33 378 489 1004 1262 2260 2475 3160 4153
Size: 345 111 515 258 998 215 685 993
Cuts at: 4153 4344 33
Size: 191 1121
Fragments arranged by size:
1121 998 993 685 515 345 258 215
191 111
SfoI GGC'GCC
Cuts at: 2864 3112 2864
Size: 248 5184
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
SmaI CCC'GGG
Cuts at: 1313 2454 1313
Size: 1141 4291
SmlI C'TyrA_G
Cuts at: 442 451 1417 1678 1891 2587 3502 3994 4256
Size: 9 966 261 213 696 915 492 262
Cuts at: 4256 442
Size: 1618
Fragments arranged by size:
1618 966 915 696 492 262 261 213
9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5432
SphI G_CATG'C
Cuts at: 589 2485 2985 589
Size: 1896 500 3036
Fragments arranged by size:
3036 1896 500
StuI AGG'CCT
Cuts at: 808 869 808
Size: 61 5371
StyI C'CwwG_G
Cuts at: 107 2001 2566 2920 107
Size: 1894 565 354 2619
Fragments arranged by size:
2619 1894 565 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1291 1614 1999 2177 3015 3148 3607 3816 1291
Size: 323 385 178 838 133 459 209 2907
Fragments arranged by size:
2907 838 459 385 323 209 178 133
TatI w'GTAC_w
Cuts at: 515 1661 4947 515
Size: 1146 3286 1000
Fragments arranged by size:
3286 1146 1000
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 146 178 404 436 597 629 1043 1075 1814
Size: 32 226 32 161 32 414 32 739
Cuts at: 1814 1846 2129 2161 4949 4981 146
Size: 32 283 32 2788 32 597
Fragments arranged by size:
2788 739 597 414 283 226 161 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3229 3229
Size: 5432
XbaI T'CTAG_A
Cuts at: 2463 2463
Size: 5432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 931 2927 931
Size: 1996 3436
XhoI C'TCGA_G
Cuts at: 1417 1891 1417
Size: 474 4958
XmaI C'CCGG_G
Cuts at: 1311 2452 1311
Size: 1141 4291
XmnI GAAnn'nnTTC
Cuts at: 1477 2036 1477
Size: 559 4873
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PmeI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AloI AscI AvrII BaeI BbsI BfrBI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PfoI PmlI PshAI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI