Restriction map for
pF1KB8002
(Circular) (Six-base) MAPSORT of: pF1KB8002.seq Check: 1646 from: 1 to: 4241
pF1KB8002 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 1064 2066 2498 3245 3513 587
Size: 477 1002 432 747 268 1315
Fragments arranged by size:
1315 1002 747 477 432 268
AflII C'TTAA_G
Cuts at: 970 970
Size: 4241
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AleI CACnn'nnGTG
Cuts at: 710 710
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1087 1546 3113 1087
Size: 459 1567 2215
Fragments arranged by size:
2215 1567 459
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 139 190 371 741 933 1209 1245 3594 3850
Size: 51 181 370 192 276 36 2349 256
Cuts at: 3850 139
Size: 530
Fragments arranged by size:
2349 530 370 276 256 192 181 51
36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 751 1257 1671 1919 1954 751
Size: 506 414 248 35 3038
Fragments arranged by size:
3038 506 414 248 35
BanII G_rGCy'C
Cuts at: 1255 2285 1255
Size: 1030 3211
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbsI GAAGACnn'nnnn_
Cuts at: 610 1164 610
Size: 554 3687
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1230 1761 1230
Size: 531 3710
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 1866 1959 3015 1866
Size: 93 1056 3092
Fragments arranged by size:
3092 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1162 1453 1534 1869 4013 1162
Size: 291 81 335 2144 1390
Fragments arranged by size:
2144 1390 335 291 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 831 3945 831
Size: 3114 1127
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1045 1045
Size: 4241
BseYI C'CCAG_C
Cuts at: 1666 2424 3001 3497 1666
Size: 758 577 496 2410
Fragments arranged by size:
2410 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1255 2033 2223 3015 1255
Size: 778 190 792 2481
Fragments arranged by size:
2481 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1255 1866 1959 2033 2223 2285 3015 1255
Size: 611 93 74 190 62 730 2481
Fragments arranged by size:
2481 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 195 1026 3417 195
Size: 831 2391 1019
Fragments arranged by size:
2391 1019 831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 314 2153 314
Size: 1839 2402
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstEII G'GTnAC_C
Cuts at: 514 514
Size: 4241
BstYI r'GATC_y
Cuts at: 1 471 1266 1733 1756 2091 2337 3349 3435
Size: 470 795 467 23 335 246 1012 86
Cuts at: 3435 3447 3793 1
Size: 12 346 449
Fragments arranged by size:
1012 795 470 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 605 1484 605
Size: 879 3362
DraI TTT'AAA
Cuts at: 1119 1240 1119
Size: 121 4120
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 1826 2000 2391 2418 3763 1826
Size: 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1112 2449 2759 2905 4051 127
Size: 985 1337 310 146 1146 317
Fragments arranged by size:
1337 1146 985 317 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 1064 2066 2498 3245 3513 587
Size: 477 1002 432 747 268 1315
Fragments arranged by size:
1315 1002 747 477 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 265 265
Size: 4241
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 933 1245 933
Size: 312 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 414 446 523 555 1006 1038 1057 1089 2083
Size: 32 77 32 451 32 19 32 994
Cuts at: 2083 2113 2115 2145 414
Size: 30 2 30 2510
Fragments arranged by size:
2510 994 451 77 32 32 32 32
30 30 19 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2912 3096 2912
Size: 184 4057
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 552 710 2230 2357 552
Size: 158 1520 127 2436
Fragments arranged by size:
2436 1520 158 127
MspA1I CmG'CkG
Cuts at: 319 1352 1666 2026 3039 3284 319
Size: 1033 314 360 1013 245 1276
Fragments arranged by size:
1276 1033 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 107 107
Size: 4241
NdeI CA'TA_TG
Cuts at: 987 987
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 319 1666 2026 319
Size: 1347 360 2534
Fragments arranged by size:
2534 1347 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 830 1284 1969 2962 3153 33
Size: 797 454 685 993 191 1121
Fragments arranged by size:
1121 993 797 685 454 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 970 1396 2311 2803 3065 970
Size: 426 915 492 262 2146
Fragments arranged by size:
2146 915 492 426 262
SpeI A'CTAG_T
Cuts at: 67 709 67
Size: 642 3599
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StuI AGG'CCT
Cuts at: 180 180
Size: 4241
StyI C'CwwG_G
Cuts at: 107 1375 1729 107
Size: 1268 354 2619
Fragments arranged by size:
2619 1268 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1824 1957 2416 2625 1824
Size: 133 459 209 3440
Fragments arranged by size:
3440 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1204 1272 1204
Size: 68 4173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 992 1736 992
Size: 744 3497
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
XmnI GAAnn'nnTTC
Cuts at: 572 572
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI