Restriction map for
pF1KB8001
(Circular) (Six-base) MAPSORT of: pF1KB8001.seq Check: 9882 from: 1 to: 3908
pF1KB8001 3908 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 554 946 554
Size: 392 3516
Acc65I G'GTAC_C
Cuts at: 924 924
Size: 3908
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 1733 2165 2912 3180 425
Size: 1308 432 747 268 1153
Fragments arranged by size:
1308 1153 747 432 268
AflIII A'CryG_T
Cuts at: 1260 2364 3341 1260
Size: 1104 977 1827
Fragments arranged by size:
1827 1104 977
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AlwNI CAG_nnn'CTG
Cuts at: 403 814 856 1213 2780 403
Size: 411 42 357 1567 1531
Fragments arranged by size:
1567 1531 411 357 42
ApaI G_GGCC'C
Cuts at: 821 821
Size: 3908
ApaLI G'TGCA_C
Cuts at: 2678 2678
Size: 3908
ApoI r'AATT_y
Cuts at: 912 3261 3517 912
Size: 2349 256 1303
Fragments arranged by size:
2349 1303 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 746 928 746
Size: 182 3726
BamHI G'GATC_C
Cuts at: 933 933
Size: 3908
BanI G'GyrC_C
Cuts at: 635 924 1338 1586 1621 635
Size: 289 414 248 35 2922
Fragments arranged by size:
2922 414 289 248 35
BanII G_rGCy'C
Cuts at: 293 821 922 1952 293
Size: 528 101 1030 2249
Fragments arranged by size:
2249 1030 528 101
BbeI G_GCGC'C
Cuts at: 639 1342 1590 639
Size: 703 248 2957
Fragments arranged by size:
2957 703 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3874
BciVI GTATCCnnnnn_n'
Cuts at: 336 851 1806 2567 3908 336
Size: 515 955 761 1341 336
Fragments arranged by size:
1341 955 761 515 336
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BglII A'GATC_T
Cuts at: 1423 3460 1423
Size: 2037 1871
BlpI GC'TnA_GC
Cuts at: 1020 1020
Size: 3908
Bme1580I G_kGCm'C
Cuts at: 754 821 1533 1626 2682 754
Size: 67 712 93 1056 1980
Fragments arranged by size:
1980 1056 712 93 67
BmrI ACTGGGnnnn_n'
Cuts at: 1120 1201 1536 3680 1120
Size: 81 335 2144 1348
Fragments arranged by size:
2144 1348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 172 2275 172
Size: 2103 1805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 3908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1963 2455 2753 1084
Size: 879 492 298 2239
Fragments arranged by size:
2239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3612 3612
Size: 3908
BsaAI yAC'GTr
Cuts at: 755 1261 755
Size: 506 3402
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 636 740 1339 1587 2289 3785 636
Size: 104 599 248 702 1496 759
Fragments arranged by size:
1496 759 702 599 248 104
BsaWI w'CCGG_w
Cuts at: 419 1097 1618 2296 2570 2717 3128 419
Size: 678 521 678 274 147 411 1199
Fragments arranged by size:
1199 678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 337
Size: 30 3878
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 667
Size: 3908
BseYI C'CCAG_C
Cuts at: 814 1333 2091 2668 3164 814
Size: 519 758 577 496 1558
Fragments arranged by size:
1558 758 577 519 496
BsiEI CG_ry'CG
Cuts at: 104 838 1450 1496 2704 104
Size: 734 612 46 1208 1308
Fragments arranged by size:
1308 1208 734 612 46
BsiHKAI G_wGCw'C
Cuts at: 863 922 1700 1890 2682 863
Size: 59 778 190 792 2089
Fragments arranged by size:
2089 792 778 190 59
BsmI GAATG_Cn'
Cuts at: 312 312
Size: 3908
Bsp1286I G_dGCh'C
Cuts at: 293 754 821 863 922 1533 1626 1700 1890
Size: 461 67 42 59 611 93 74 190
Cuts at: 1890 1952 2682 293
Size: 62 730 1519
Fragments arranged by size:
1519 730 611 461 190 93 74 67
62 59 42
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 3084 3084
Size: 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 958 1172 1474 1855 958
Size: 214 302 381 3011
Fragments arranged by size:
3011 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2200 2254 3738 3903 50
Size: 2150 54 1484 165 55
Fragments arranged by size:
2150 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 191 1820 191
Size: 1629 2279
BsrFI r'CCGG_y
Cuts at: 1906 2087 2296 1906
Size: 181 209 3518
Fragments arranged by size:
3518 209 181
BssSI C'ACGA_G
Cuts at: 2179 2537 2179
Size: 358 3550
BstAPI GCAn_nnn'nTGC
Cuts at: 1285 1285
Size: 3908
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstEII G'GTnAC_C
Cuts at: 360 360
Size: 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 240 260 357 240
Size: 20 97 3791
Fragments arranged by size:
3791 97 20
BstYI r'GATC_y
Cuts at: 1 933 1400 1423 1758 2004 3016 3102 3114
Size: 932 467 23 335 246 1012 86 12
Cuts at: 3114 3460 1
Size: 346 449
Fragments arranged by size:
1012 932 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 356 594 716 356
Size: 238 122 3548
Fragments arranged by size:
3548 238 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 628 1858 2039 2202 628
Size: 1230 181 163 2334
Fragments arranged by size:
2334 1230 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1151
Size: 3908
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 1614 2472 1614
Size: 858 3050
EaeI y'GGCC_r
Cuts at: 140 371 835 1493 1667 2058 2085 3430 140
Size: 231 464 658 174 391 27 1345 618
Fragments arranged by size:
1345 658 618 464 391 231 174 27
EagI C'GGCC_G
Cuts at: 835 1493 835
Size: 658 3250
EarI CTCTTCn'nnn_
Cuts at: 179 614 698 829 1931 2141 179
Size: 435 84 131 1102 210 1946
Fragments arranged by size:
1946 1102 435 210 131 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2116 2426 2572 3718 2116
Size: 310 146 1146 2306
Fragments arranged by size:
2306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 425 1733 2165 2912 3180 187
Size: 238 1308 432 747 268 915
Fragments arranged by size:
1308 915 747 432 268 238
EcoO109I rG'GnC_Cy
Cuts at: 246 818 1047 246
Size: 572 229 3107
Fragments arranged by size:
3107 572 229
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 639 800 1342 1590 2612 3591 639
Size: 161 542 248 1022 979 956
Fragments arranged by size:
1022 979 956 542 248 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 337 342 369 712 744 1750 1780 1782
Size: 27 5 27 343 32 1006 30 2
Cuts at: 1782 1812 310
Size: 30 2406
Fragments arranged by size:
2406 1006 343 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 761 947 761
Size: 186 3722
KasI G'GCGC_C
Cuts at: 635 1338 1586 635
Size: 703 248 2957
Fragments arranged by size:
2957 703 248
KpnI G_GTAC'C
Cuts at: 928 928
Size: 3908
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 395 416 437 2579 2763 221
Size: 174 21 21 2142 184 1366
Fragments arranged by size:
2142 1366 184 174 21 21
MscI TGG'CCA
Cuts at: 142 1669 142
Size: 1527 2381
MslI CAynn'nnrTG
Cuts at: 1897 2024 1897
Size: 127 3781
MspA1I CmG'CkG
Cuts at: 114 596 814 834 1019 1333 1693 2706 2951
Size: 482 218 20 185 314 360 1013 245
Cuts at: 2951 114
Size: 1071
Fragments arranged by size:
1071 1013 482 360 314 245 218 185
20
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 3908
NarI GG'CG_CC
Cuts at: 636 1339 1587 636
Size: 703 248 2957
Fragments arranged by size:
2957 703 248
NcoI C'CATG_G
Cuts at: 716 716
Size: 3908
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 3908
NspI r_CATG'y
Cuts at: 961 1461 2368 961
Size: 500 907 2501
Fragments arranged by size:
2501 907 500
PasI CC'CwG_GG
Cuts at: 850 850
Size: 3908
PciI A'CATG_T
Cuts at: 2364 2364
Size: 3908
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PmlI CAC'GTG
Cuts at: 755 755
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3876
PspOMI G'GGCC_C
Cuts at: 817 817
Size: 3908
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 256 288 256
Size: 32 3876
PstI C_TGCA'G
Cuts at: 131 955 1640 131
Size: 824 685 2399
Fragments arranged by size:
2399 824 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 814 1333 1693 814
Size: 519 360 3029
Fragments arranged by size:
3029 519 360
RsrII CG'GwC_CG
Cuts at: 459 2103 459
Size: 1644 2264
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SacII CC_GC'GG
Cuts at: 597 597
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
SbfI CC_TGCA'GG
Cuts at: 955 955
Size: 3908
ScaI AGT'ACT
Cuts at: 3425 3425
Size: 3908
SexAI A'CCwGG_T
Cuts at: 235 235
Size: 3908
SfcI C'TryA_G
Cuts at: 33 127 951 1636 2629 2820 33
Size: 94 824 685 993 191 1121
Fragments arranged by size:
1121 993 824 685 191 94
SfoI GGC'GCC
Cuts at: 637 1340 1588 637
Size: 703 248 2957
Fragments arranged by size:
2957 703 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 748 930 748
Size: 182 3726
SmlI C'TyrA_G
Cuts at: 1063 1978 2470 2732 1063
Size: 915 492 262 2239
Fragments arranged by size:
2239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 961 1461 961
Size: 500 3408
StuI AGG'CCT
Cuts at: 880 880
Size: 3908
StyI C'CwwG_G
Cuts at: 716 1042 1396 716
Size: 326 354 3228
Fragments arranged by size:
3228 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1491 1624 2083 2292 1491
Size: 133 459 209 3107
Fragments arranged by size:
3107 459 209 133
TatI w'GTAC_w
Cuts at: 3423 3423
Size: 3908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 3876
Tth111I GACn'n_nGTC
Cuts at: 1705 1705
Size: 3908
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1403 1403
Size: 3908
XmaI C'CCGG_G
Cuts at: 746 928 746
Size: 182 3726
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PmlI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PpuMI PshAI PsiI PspXI SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI