Restriction map for
pF1KB7996
(Circular) (Six-base) MAPSORT of: pF1KB7996.seq Check: 2888 from: 1 to: 4523
pF1KB7996 4523 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 594 816 837 846 594
Size: 222 21 9 4271
Fragments arranged by size:
4271 222 21 9
AccI GT'mk_AC
Cuts at: 1561 1561
Size: 4523
Acc65I G'GTAC_C
Cuts at: 1539 1539
Size: 4523
AclI AA'CG_TT
Cuts at: 4363 4363
Size: 4523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 2348 2780 3527 3795 126
Size: 2222 432 747 268 854
Fragments arranged by size:
2222 854 747 432 268
AfeI AGC'GCT
Cuts at: 899 899
Size: 4523
AflIII A'CryG_T
Cuts at: 1875 2979 3956 1875
Size: 1104 977 2442
Fragments arranged by size:
2442 1104 977
AgeI A'CCGG_T
Cuts at: 2911 2911
Size: 4523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4523
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1129 1161 1129
Size: 32 4491
AlwNI CAG_nnn'CTG
Cuts at: 408 549 1828 3395 408
Size: 141 1279 1567 1536
Fragments arranged by size:
1567 1536 1279 141
ApaLI G'TGCA_C
Cuts at: 3293 3293
Size: 4523
ApoI r'AATT_y
Cuts at: 1527 3876 4132 1527
Size: 2349 256 1918
Fragments arranged by size:
2349 1918 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4523
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
AvaI C'yCGr_G
Cuts at: 193 258 296 503 883 1189 1543 193
Size: 65 38 207 380 306 354 3173
Fragments arranged by size:
3173 380 354 306 207 65 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 147 180 147
Size: 33 4490
BamHI G'GATC_C
Cuts at: 1548 1548
Size: 4523
BanI G'GyrC_C
Cuts at: 310 1229 1539 1953 2201 2236 310
Size: 919 310 414 248 35 2597
Fragments arranged by size:
2597 919 414 310 248 35
BanII G_rGCy'C
Cuts at: 250 265 419 1253 1537 2567 250
Size: 15 154 834 284 1030 2206
Fragments arranged by size:
2206 1030 834 284 154 15
BbeI G_GCGC'C
Cuts at: 314 1957 2205 314
Size: 1643 248 2632
Fragments arranged by size:
2632 1643 248
BbsI GAAGACnn'nnnn_
Cuts at: 1420 1493 1420
Size: 73 4450
BbvCI CC'TCA_GC
Cuts at: 901 901
Size: 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 927 961 927
Size: 34 4489
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3182 4523 2421
Size: 761 1341 2421
Fragments arranged by size:
2421 1341 761
BclI T'GATC_A
Cuts at: 1424 2043 1424
Size: 619 3904
BglI GCCn_nnn'nGGC
Cuts at: 245 1156 245
Size: 911 3612
BglII A'GATC_T
Cuts at: 2038 4075 2038
Size: 2037 2486
BlpI GC'TnA_GC
Cuts at: 493 1635 493
Size: 1142 3381
Bme1580I G_kGCm'C
Cuts at: 1337 2148 2241 3297 1337
Size: 811 93 1056 2563
Fragments arranged by size:
2563 1056 811 93
BmgBI CAC'GTC
Cuts at: 717 717
Size: 4523
BmrI ACTGGGnnnn_n'
Cuts at: 1735 1816 2151 4295 1735
Size: 81 335 2144 1963
Fragments arranged by size:
2144 1963 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 481 592 1156 1174 1327 213
Size: 268 111 564 18 153 3409
Fragments arranged by size:
3409 564 268 153 111 18
Bpu10I CC'TnA_GC
Cuts at: 901 1117 901
Size: 216 4307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 775 1699 2578 3070 3368 628
Size: 147 924 879 492 298 1783
Fragments arranged by size:
1783 924 879 492 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 812 1432 4227 812
Size: 620 2795 1108
Fragments arranged by size:
2795 1108 620
BsaAI yAC'GTr
Cuts at: 1876 1876
Size: 4523
BsaBI GATnn'nnATC
Cuts at: 6 2037 4074 6
Size: 2031 2037 455
Fragments arranged by size:
2037 2031 455
BsaHI Gr'CG_yC
Cuts at: 311 1056 1954 2202 2904 4400 311
Size: 745 898 248 702 1496 434
Fragments arranged by size:
1496 898 745 702 434 248
BsaWI w'CCGG_w
Cuts at: 1004 1712 2233 2911 3185 3332 3743 1004
Size: 708 521 678 274 147 411 1784
Fragments arranged by size:
1784 708 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 949 979 1129 1159 949
Size: 30 150 30 4313
Fragments arranged by size:
4313 150 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 255 673 676 820 823 973 1153 1207 1210
Size: 418 3 144 3 150 180 54 3
Cuts at: 1210 1231 1243 1330 255
Size: 21 12 87 3448
Fragments arranged by size:
3448 418 180 150 144 87 54 21
12 3 3 3
BseYI C'CCAG_C
Cuts at: 243 1948 2706 3283 3779 243
Size: 1705 758 577 496 987
Fragments arranged by size:
1705 987 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 784 871 622
Size: 162 87 4274
Fragments arranged by size:
4274 162 87
BsiEI CG_ry'CG
Cuts at: 104 426 2065 2111 3319 104
Size: 322 1639 46 1208 1308
Fragments arranged by size:
1639 1308 1208 322 46
BsiHKAI G_wGCw'C
Cuts at: 265 419 692 704 1253 1537 2315 2505 3297
Size: 154 273 12 549 284 778 190 792
Cuts at: 3297 265
Size: 1491
Fragments arranged by size:
1491 792 778 549 284 273 190 154
12
BsmBI CGTCTCn'nnnn_
Cuts at: 658 658
Size: 4523
Bsp1286I G_dGCh'C
Cuts at: 250 265 419 692 704 1253 1337 1537 2148
Size: 15 154 273 12 549 84 200 611
Cuts at: 2148 2241 2315 2505 2567 3297 250
Size: 93 74 190 62 730 1476
Fragments arranged by size:
1476 730 611 549 273 200 190 154
93 84 74 62 15 12
BspEI T'CCGG_A
Cuts at: 3743 3743
Size: 4523
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 594 816 830 837 846 953 1573 1787 2089
Size: 222 14 7 9 107 620 214 302
Cuts at: 2089 2470 594
Size: 381 2647
Fragments arranged by size:
2647 620 381 302 222 214 107 14
9 7
BsrBI CCG'CTC
Cuts at: 50 301 307 2815 2869 4353 4518 50
Size: 251 6 2508 54 1484 165 55
Fragments arranged by size:
2508 1484 251 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 2435 2435
Size: 4523
BsrFI r'CCGG_y
Cuts at: 136 516 2521 2702 2911 136
Size: 380 2005 181 209 1748
Fragments arranged by size:
2005 1748 380 209 181
BssHII G'CGCG_C
Cuts at: 520 522 914 520
Size: 2 392 4129
Fragments arranged by size:
4129 392 2
BssSI C'ACGA_G
Cuts at: 697 802 1012 2794 3152 697
Size: 105 210 1782 358 2068
Fragments arranged by size:
2068 1782 358 210 105
BstAPI GCAn_nnn'nTGC
Cuts at: 408 549 1900 408
Size: 141 1351 3031
Fragments arranged by size:
3031 1351 141
BstBI TT'CG_AA
Cuts at: 2884 2884
Size: 4523
BstYI r'GATC_y
Cuts at: 1 1061 1325 1548 2015 2038 2373 2619 3631
Size: 1060 264 223 467 23 335 246 1012
Cuts at: 3631 3717 3729 4075 1
Size: 86 12 346 449
Fragments arranged by size:
1060 1012 467 449 346 335 264 246
223 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1234 1379 1234
Size: 145 4378
BtgI C'CryG_G
Cuts at: 350 872 350
Size: 522 4001
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 915 2473 2654 2817 915
Size: 1558 181 163 2621
Fragments arranged by size:
2621 1558 181 163
BtsI GCAGTG_nn'
Cuts at: 1491 1766 1491
Size: 275 4248
DraI TTT'AAA
Cuts at: 1522 1522
Size: 4523
DrdI GACnn_nn'nnGTC
Cuts at: 2229 3087 2229
Size: 858 3665
EaeI y'GGCC_r
Cuts at: 353 380 2108 2282 2673 2700 4045 353
Size: 27 1728 174 391 27 1345 831
Fragments arranged by size:
1728 1345 831 391 174 27 27
EagI C'GGCC_G
Cuts at: 2108 2108
Size: 4523
EarI CTCTTCn'nnn_
Cuts at: 709 2546 2756 709
Size: 1837 210 2476
Fragments arranged by size:
2476 1837 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 640 970 2731 3041 3187 4333 640
Size: 330 1761 310 146 1146 830
Fragments arranged by size:
1761 1146 830 330 310 146
EcoICRI GAG'CTC
Cuts at: 263 417 1251 1535 263
Size: 154 834 284 3251
Fragments arranged by size:
3251 834 284 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 213 481 592 1156 1174 1327 2348 2780
Size: 87 268 111 564 18 153 1021 432
Cuts at: 2780 3527 3795 126
Size: 747 268 854
Fragments arranged by size:
1021 854 747 564 432 268 268 153
111 87 18
EcoNI CCTnn'n_nnAGG
Cuts at: 367 949 1024 367
Size: 582 75 3866
Fragments arranged by size:
3866 582 75
EcoO109I rG'GnC_Cy
Cuts at: 197 360 630 1017 1662 197
Size: 163 270 387 645 3058
Fragments arranged by size:
3058 645 387 270 163
EcoRI G'AATT_C
Cuts at: 1527 1527
Size: 4523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 2542 471
Size: 2071 2452
FspI TGC'GCA
Cuts at: 2304 2304
Size: 4523
HaeII r_GCGC'y
Cuts at: 314 339 643 901 1114 1181 1957 2205 3227
Size: 25 304 258 213 67 776 248 1022
Cuts at: 3227 4206 314
Size: 979 631
Fragments arranged by size:
1022 979 776 631 304 258 248 213
67 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 750 782 1046 1053 1078 1085 2365 2395 2397
Size: 32 264 7 25 7 1280 30 2
Cuts at: 2397 2427 750
Size: 30 2846
Fragments arranged by size:
2846 1280 264 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 1562 1562
Size: 4523
KasI G'GCGC_C
Cuts at: 310 1953 2201 310
Size: 1643 248 2632
Fragments arranged by size:
2632 1643 248
KpnI G_GTAC'C
Cuts at: 1543 1543
Size: 4523
MluI A'CGCG_T
Cuts at: 3956 3956
Size: 4523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 1016 3194 3378 635
Size: 381 2178 184 1780
Fragments arranged by size:
2178 1780 381 184
MscI TGG'CCA
Cuts at: 382 2284 382
Size: 1902 2621
MslI CAynn'nnrTG
Cuts at: 2512 2639 2512
Size: 127 4396
MspA1I CmG'CkG
Cuts at: 534 1634 1948 2308 3321 3566 534
Size: 1100 314 360 1013 245 1491
Fragments arranged by size:
1491 1100 1013 360 314 245
NaeI GCC'GGC
Cuts at: 138 518 2704 138
Size: 380 2186 1957
Fragments arranged by size:
2186 1957 380
NarI GG'CG_CC
Cuts at: 311 1954 2202 311
Size: 1643 248 2632
Fragments arranged by size:
2632 1643 248
NcoI C'CATG_G
Cuts at: 350 350
Size: 4523
NgoMIV G'CCGG_C
Cuts at: 136 516 2702 136
Size: 380 2186 1957
Fragments arranged by size:
2186 1957 380
NspI r_CATG'y
Cuts at: 1576 2076 2983 1576
Size: 500 907 3116
Fragments arranged by size:
3116 907 500
PciI A'CATG_T
Cuts at: 2979 2979
Size: 4523
PmeI GTTT'AAAC
Cuts at: 1522 1522
Size: 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 769 801 1129 1161 3662 3694 769
Size: 32 328 32 2501 32 1598
Fragments arranged by size:
2501 1598 328 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 630 630
Size: 4523
PspXI vC'TCGA_Gb
Cuts at: 1189 1189
Size: 4523
PstI C_TGCA'G
Cuts at: 413 827 950 1570 2255 413
Size: 414 123 620 685 2681
Fragments arranged by size:
2681 685 620 414 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4523
PvuII CAG'CTG
Cuts at: 534 1948 2308 534
Size: 1414 360 2749
Fragments arranged by size:
2749 1414 360
RsrII CG'GwC_CG
Cuts at: 2718 2718
Size: 4523
SacI G_AGCT'C
Cuts at: 265 419 1253 1537 265
Size: 154 834 284 3251
Fragments arranged by size:
3251 834 284 154
SalI G'TCGA_C
Cuts at: 1560 1560
Size: 4523
SapI GCTCTTCn'nnn_
Cuts at: 709 2546 2756 709
Size: 1837 210 2476
Fragments arranged by size:
2476 1837 210
SbfI CC_TGCA'GG
Cuts at: 827 950 1570 827
Size: 123 620 3780
Fragments arranged by size:
3780 620 123
ScaI AGT'ACT
Cuts at: 4040 4040
Size: 4523
SfcI C'TryA_G
Cuts at: 33 409 823 946 1566 2251 3244 3435 33
Size: 376 414 123 620 685 993 191 1121
Fragments arranged by size:
1121 993 685 620 414 376 191 123
SfoI GGC'GCC
Cuts at: 312 1955 2203 312
Size: 1643 248 2632
Fragments arranged by size:
2632 1643 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
SmaI CCC'GGG
Cuts at: 195 505 1545 195
Size: 310 1040 3173
Fragments arranged by size:
3173 1040 310
SmlI C'TyrA_G
Cuts at: 643 790 1189 1678 2593 3085 3347 643
Size: 147 399 489 915 492 262 1819
Fragments arranged by size:
1819 915 492 489 399 262 147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4523
SphI G_CATG'C
Cuts at: 1576 2076 1576
Size: 500 4023
StyI C'CwwG_G
Cuts at: 350 1657 2011 350
Size: 1307 354 2862
Fragments arranged by size:
2862 1307 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 2106 2239 2698 2907 160
Size: 1946 133 459 209 1776
Fragments arranged by size:
1946 1776 459 209 133
TatI w'GTAC_w
Cuts at: 4038 4038
Size: 4523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4491
Tth111I GACn'n_nGTC
Cuts at: 1464 2320 1464
Size: 856 3667
XbaI T'CTAG_A
Cuts at: 1554 1554
Size: 4523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2018
Size: 4523
XhoI C'TCGA_G
Cuts at: 1189 1189
Size: 4523
XmaI C'CCGG_G
Cuts at: 193 503 1543 193
Size: 310 1040 3173
Fragments arranged by size:
3173 1040 310
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI ApaI AscI AvrII BfrBI
BmtI BsiWI BsmI BsrGI BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI