Restriction map for
pF1KB7990
(Circular) (Six-base) MAPSORT of: pF1KB7990.seq Check: 4096 from: 1 to: 4091
pF1KB7990 4091 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 927 927
Size: 4091
AccI GT'mk_AC
Cuts at: 1129 1129
Size: 4091
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1916 2348 3095 3363 491
Size: 1425 432 747 268 1219
Fragments arranged by size:
1425 1219 747 432 268
AfeI AGC'GCT
Cuts at: 835 835
Size: 4091
AflIII A'CryG_T
Cuts at: 1443 2547 3524 1443
Size: 1104 977 2010
Fragments arranged by size:
2010 1104 977
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AhdI GACnn_n'nnGTC
Cuts at: 141 141
Size: 4091
AleI CACnn'nnGTG
Cuts at: 433 433
Size: 4091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 543 543
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 285 718 1396 2963 285
Size: 433 678 1567 1413
Fragments arranged by size:
1567 1413 678 433
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 211 1095 3444 3700 211
Size: 884 2349 256 602
Fragments arranged by size:
2349 884 602 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 124 146 691 1111 124
Size: 22 545 420 3104
Fragments arranged by size:
3104 545 420 22
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 427 934 1107 1521 1769 1804 427
Size: 507 173 414 248 35 2714
Fragments arranged by size:
2714 507 414 248 173 35
BanII G_rGCy'C
Cuts at: 782 887 1105 2135 782
Size: 105 218 1030 2738
Fragments arranged by size:
2738 1030 218 105
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BbsI GAAGACnn'nnnn_
Cuts at: 942 942
Size: 4091
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 311 413 1611 311
Size: 102 1198 2791
Fragments arranged by size:
2791 1198 102
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 620 1203 620
Size: 583 3508
Bme1580I G_kGCm'C
Cuts at: 937 1716 1809 2865 937
Size: 779 93 1056 2163
Fragments arranged by size:
2163 1056 779 93
BmrI ACTGGGnnnn_n'
Cuts at: 667 1303 1384 1719 3863 667
Size: 636 81 335 2144 895
Fragments arranged by size:
2144 895 636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 201 2458 201
Size: 2257 1834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 604 667 991 171
Size: 433 63 324 3271
Fragments arranged by size:
3271 433 324 63
Bpu10I CC'TnA_GC
Cuts at: 234 234
Size: 4091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 4091
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 540 1522 1770 2472 3968 540
Size: 982 248 702 1496 663
Fragments arranged by size:
1496 982 702 663 248
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 640 670 964 994 580
Size: 30 30 30 294 30 3677
Fragments arranged by size:
3677 294 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 462 490 493 592 601 604 709 895 462
Size: 28 3 99 9 3 105 186 3658
Fragments arranged by size:
3658 186 105 99 28 9 3 3
BseYI C'CCAG_C
Cuts at: 711 830 862 1516 2274 2851 3347 711
Size: 119 32 654 758 577 496 1455
Fragments arranged by size:
1455 758 654 577 496 119 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 874 319
Size: 555 3536
BsiEI CG_ry'CG
Cuts at: 104 706 1633 1679 2887 104
Size: 602 927 46 1208 1308
Fragments arranged by size:
1308 1208 927 602 46
BsiHKAI G_wGCw'C
Cuts at: 1105 1883 2073 2865 1105
Size: 778 190 792 2331
Fragments arranged by size:
2331 792 778 190
BsmI GAATG_Cn'
Cuts at: 244 244
Size: 4091
Bsp1286I G_dGCh'C
Cuts at: 782 887 937 1105 1716 1809 1883 2073 2135
Size: 105 50 168 611 93 74 190 62
Cuts at: 2135 2865 782
Size: 730 2008
Fragments arranged by size:
2008 730 611 190 168 105 93 74
62 50
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 927 1141 1355 1657 2038 293
Size: 634 214 214 302 381 2346
Fragments arranged by size:
2346 634 381 302 214 214
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 857 2003 857
Size: 1146 2945
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 732 1468 732
Size: 736 3355
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstEII G'GTnAC_C
Cuts at: 906 906
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 1077 1077
Size: 4091
BstYI r'GATC_y
Cuts at: 1 1116 1583 1606 1941 2187 3199 3285 3297
Size: 1115 467 23 335 246 1012 86 12
Cuts at: 3297 3643 1
Size: 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 434 434
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 246 295 744 1334 246
Size: 49 449 590 3003
Fragments arranged by size:
3003 590 449 49
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 201 1676 1850 2241 2268 3613 201
Size: 1475 174 391 27 1345 679
Fragments arranged by size:
1475 1345 679 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 467 782 2114 2324 467
Size: 315 1332 210 2234
Fragments arranged by size:
2234 1332 315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 583 2299 2609 2755 3901 583
Size: 1716 310 146 1146 773
Fragments arranged by size:
1716 1146 773 310 146
EcoICRI GAG'CTC
Cuts at: 1103 1103
Size: 4091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 491 604 667 991 1916 2348 3095 3363
Size: 320 113 63 324 925 432 747 268
Cuts at: 3363 171
Size: 899
Fragments arranged by size:
925 899 747 432 324 320 268 113
63
EcoNI CCTnn'n_nnAGG
Cuts at: 233 607 787 973 233
Size: 374 180 186 3351
Fragments arranged by size:
3351 374 186 180
EcoO109I rG'GnC_Cy
Cuts at: 282 1230 282
Size: 948 3143
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 170 736 1872 170
Size: 566 1136 2389
Fragments arranged by size:
2389 1136 566
HaeII r_GCGC'y
Cuts at: 702 761 837 1525 1773 2795 3774 702
Size: 59 76 688 248 1022 979 1019
Fragments arranged by size:
1022 1019 979 688 248 76 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 448 480 580 612 688 720 784
Size: 32 289 32 100 32 76 32 64
Cuts at: 784 816 964 996 1033 1065 1933 1963 1965
Size: 32 148 32 37 32 868 30 2
Cuts at: 1965 1995 127
Size: 30 2223
Fragments arranged by size:
2223 868 289 148 100 76 64 37
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 2762 2946 350
Size: 2412 184 1495
Fragments arranged by size:
2412 1495 184
MscI TGG'CCA
Cuts at: 1852 1852
Size: 4091
MslI CAynn'nnrTG
Cuts at: 319 433 658 904 2080 2207 319
Size: 114 225 246 1176 127 2203
Fragments arranged by size:
2203 1176 246 225 127 114
MspA1I CmG'CkG
Cuts at: 158 191 462 1037 1202 1516 1876 2889 3134
Size: 33 271 575 165 314 360 1013 245
Cuts at: 3134 158
Size: 1115
Fragments arranged by size:
1115 1013 575 360 314 271 245 165
33
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PasI CC'CwG_GG
Cuts at: 718 718
Size: 4091
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PflMI CCAn_nnn'nTGG
Cuts at: 406 718 406
Size: 312 3779
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PpuMI rG'GwC_Cy
Cuts at: 282 282
Size: 4091
PstI C_TGCA'G
Cuts at: 290 522 566 608 629 728 1138 1823 290
Size: 232 44 42 21 99 410 685 2558
Fragments arranged by size:
2558 685 410 232 99 44 42 21
PvuI CG_AT'CG
Cuts at: 104 706 104
Size: 602 3489
PvuII CAG'CTG
Cuts at: 462 1516 1876 462
Size: 1054 360 2677
Fragments arranged by size:
2677 1054 360
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 1105 1105
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 286 351 518 562 604 625 724 761
Size: 253 65 167 44 42 21 99 37
Cuts at: 761 1134 1819 2812 3003 33
Size: 373 685 993 191 1121
Fragments arranged by size:
1121 993 685 373 253 191 167 99
65 44 42 37 21
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 126 148 1113 126
Size: 22 965 3104
Fragments arranged by size:
3104 965 22
SmlI C'TyrA_G
Cuts at: 1246 2161 2653 2915 1246
Size: 915 492 262 2422
Fragments arranged by size:
2422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StuI AGG'CCT
Cuts at: 602 602
Size: 4091
StyI C'CwwG_G
Cuts at: 1010 1225 1579 1010
Size: 215 354 3522
Fragments arranged by size:
3522 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1674 1807 2266 2475 1674
Size: 133 459 209 3290
Fragments arranged by size:
3290 459 209 133
TatI w'GTAC_w
Cuts at: 3606 3606
Size: 4091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 274 306 3608 3640 163
Size: 32 79 32 3302 32 614
Fragments arranged by size:
3302 614 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 124 146 1111 124
Size: 22 965 3104
Fragments arranged by size:
3104 965 22
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI