Restriction map for
pF1KB7984
(Circular) (Six-base) MAPSORT of: pF1KB7984.seq Check: 3966 from: 1 to: 4085
pF1KB7984 4085 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 1101 1101
Size: 4085
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 3089 3357 1910
Size: 432 747 268 2638
Fragments arranged by size:
2638 747 432 268
AflIII A'CryG_T
Cuts at: 461 635 953 955 1437 2541 3518 461
Size: 174 318 2 482 1104 977 1028
Fragments arranged by size:
1104 1028 977 482 318 174 2
AgeI A'CCGG_T
Cuts at: 2473 2473
Size: 4085
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1021 1053 1021
Size: 32 4053
AlwNI CAG_nnn'CTG
Cuts at: 426 1390 2957 426
Size: 964 1567 1554
Fragments arranged by size:
1567 1554 964
ApaLI G'TGCA_C
Cuts at: 274 2855 274
Size: 2581 1504
ApoI r'AATT_y
Cuts at: 888 1089 3438 3694 888
Size: 201 2349 256 1279
Fragments arranged by size:
2349 1279 256 201
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 509 831 963 1105 509
Size: 322 132 142 3489
Fragments arranged by size:
3489 322 142 132
BamHI G'GATC_C
Cuts at: 1110 1110
Size: 4085
BanI G'GyrC_C
Cuts at: 352 807 897 1101 1515 1763 1798 352
Size: 455 90 204 414 248 35 2639
Fragments arranged by size:
2639 455 414 248 204 90 35
BanII G_rGCy'C
Cuts at: 617 838 1099 2129 617
Size: 221 261 1030 2573
Fragments arranged by size:
2573 1030 261 221
BbeI G_GCGC'C
Cuts at: 356 1519 1767 356
Size: 1163 248 2674
Fragments arranged by size:
2674 1163 248
BbvCI CC'TCA_GC
Cuts at: 813 813
Size: 4085
BciVI GTATCCnnnnn_n'
Cuts at: 1983 2744 4085 1983
Size: 761 1341 1983
Fragments arranged by size:
1983 1341 761
BclI T'GATC_A
Cuts at: 1605 1605
Size: 4085
BglII A'GATC_T
Cuts at: 1600 3637 1600
Size: 2037 2048
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 278 812 1710 1803 2859 278
Size: 534 898 93 1056 1504
Fragments arranged by size:
1504 1056 898 534 93
BmgBI CAC'GTC
Cuts at: 348 348
Size: 4085
BmrI ACTGGGnnnn_n'
Cuts at: 672 1297 1378 1713 3857 672
Size: 625 81 335 2144 900
Fragments arranged by size:
2144 900 625 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 289 403 448 822 987 279
Size: 10 114 45 374 165 3377
Fragments arranged by size:
3377 374 165 114 45 10
Bpu10I CC'TnA_GC
Cuts at: 813 813
Size: 4085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2140 2632 2930 1261
Size: 879 492 298 2416
Fragments arranged by size:
2416 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3789 3789
Size: 4085
BsaAI yAC'GTr
Cuts at: 956 1438 956
Size: 482 3603
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 353 827 904 1516 1764 2466 3962 353
Size: 474 77 612 248 702 1496 476
Fragments arranged by size:
1496 702 612 476 474 248 77
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2747 2894 3305 1274
Size: 521 678 274 147 411 2054
Fragments arranged by size:
2054 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 1021 1051 268
Size: 30 723 30 3302
Fragments arranged by size:
3302 723 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 243 923 165
Size: 78 680 3327
Fragments arranged by size:
3327 680 78
BseYI C'CCAG_C
Cuts at: 197 1510 2268 2845 3341 197
Size: 1313 758 577 496 941
Fragments arranged by size:
1313 941 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1045
Size: 4085
BsiEI CG_ry'CG
Cuts at: 104 170 244 1627 1673 2881 104
Size: 66 74 1383 46 1208 1308
Fragments arranged by size:
1383 1308 1208 74 66 46
BsiHKAI G_wGCw'C
Cuts at: 143 278 1099 1877 2067 2859 143
Size: 135 821 778 190 792 1369
Fragments arranged by size:
1369 821 792 778 190 135
BsmBI CGTCTCn'nnnn_
Cuts at: 177 177
Size: 4085
Bsp1286I G_dGCh'C
Cuts at: 143 278 617 812 838 1099 1710 1803 1877
Size: 135 339 195 26 261 611 93 74
Cuts at: 1877 2067 2129 2859 143
Size: 190 62 730 1369
Fragments arranged by size:
1369 730 611 339 261 195 190 135
93 74 62 26
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 3261 3261
Size: 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1135 1349 1651 2032 1135
Size: 214 302 381 3188
Fragments arranged by size:
3188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2377 2431 3915 4080 50
Size: 2327 54 1484 165 55
Fragments arranged by size:
2327 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1997 1997
Size: 4085
BsrFI r'CCGG_y
Cuts at: 1070 2083 2264 2473 1070
Size: 1013 181 209 2682
Fragments arranged by size:
2682 1013 209 181
BssSI C'ACGA_G
Cuts at: 2356 2714 2356
Size: 358 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 1462 1462
Size: 4085
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstYI r'GATC_y
Cuts at: 1 1110 1577 1600 1935 2181 3193 3279 3291
Size: 1109 467 23 335 246 1012 86 12
Cuts at: 3291 3637 1
Size: 346 449
Fragments arranged by size:
1109 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 209 893 1076 209
Size: 684 183 3218
Fragments arranged by size:
3218 684 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2035 2216 2379 2035
Size: 181 163 3741
Fragments arranged by size:
3741 181 163
BtsI GCAGTG_nn'
Cuts at: 824 1070 1328 824
Size: 246 258 3581
Fragments arranged by size:
3581 258 246
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 162 1791 2649 162
Size: 1629 858 1598
Fragments arranged by size:
1629 1598 858
EaeI y'GGCC_r
Cuts at: 167 971 1670 1844 2235 2262 3607 167
Size: 804 699 174 391 27 1345 645
Fragments arranged by size:
1345 804 699 645 391 174 27
EagI C'GGCC_G
Cuts at: 167 1670 167
Size: 1503 2582
EarI CTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 675 2293 2603 2749 3895 675
Size: 1618 310 146 1146 865
Fragments arranged by size:
1618 1146 865 310 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 289 403 448 822 987 1910 2342 3089
Size: 10 114 45 374 165 923 432 747
Cuts at: 3089 3357 279
Size: 268 1007
Fragments arranged by size:
1007 923 747 432 374 268 165 114
45 10
EcoO109I rG'GnC_Cy
Cuts at: 360 675 1224 360
Size: 315 549 3221
Fragments arranged by size:
3221 549 315
EcoRI G'AATT_C
Cuts at: 888 1089 888
Size: 201 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 145 609 2104 145
Size: 464 1495 2126
Fragments arranged by size:
2126 1495 464
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 356 1519 1767 2789 3768 356
Size: 1163 248 1022 979 673
Fragments arranged by size:
1163 1022 979 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1927 1957 1959 1989 349
Size: 32 1546 30 2 30 2445
Fragments arranged by size:
2445 1546 32 30 30 2
HincII GTy'rAC
Cuts at: 735 1124 735
Size: 389 3696
KasI G'GCGC_C
Cuts at: 352 1515 1763 352
Size: 1163 248 2674
Fragments arranged by size:
2674 1163 248
KpnI G_GTAC'C
Cuts at: 1105 1105
Size: 4085
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 1064 2756 2940 311
Size: 753 1692 184 1456
Fragments arranged by size:
1692 1456 753 184
MscI TGG'CCA
Cuts at: 973 1846 973
Size: 873 3212
MslI CAynn'nnrTG
Cuts at: 790 2074 2201 790
Size: 1284 127 2674
Fragments arranged by size:
2674 1284 127
MspA1I CmG'CkG
Cuts at: 151 393 895 1196 1510 1870 2883 3128 151
Size: 242 502 301 314 360 1013 245 1108
Fragments arranged by size:
1108 1013 502 360 314 301 245 242
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 4085
NarI GG'CG_CC
Cuts at: 353 1516 1764 353
Size: 1163 248 2674
Fragments arranged by size:
2674 1163 248
NcoI C'CATG_G
Cuts at: 209 209
Size: 4085
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 4085
NspI r_CATG'y
Cuts at: 465 639 759 795 1138 1638 2545 465
Size: 174 120 36 343 500 907 2005
Fragments arranged by size:
2005 907 500 343 174 120 36
PciI A'CATG_T
Cuts at: 461 635 2541 461
Size: 174 1906 2005
Fragments arranged by size:
2005 1906 174
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 4085
PfoI T'CCnGG_A
Cuts at: 419 419
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PmlI CAC'GTG
Cuts at: 956 956
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1021 1053 3224 3256 1021
Size: 32 2171 32 1850
Fragments arranged by size:
2171 1850 32 32
PpuMI rG'GwC_Cy
Cuts at: 360 360
Size: 4085
PshAI GACnn'nnGTC
Cuts at: 826 826
Size: 4085
PspXI vC'TCGA_Gb
Cuts at: 963 963
Size: 4085
PstI C_TGCA'G
Cuts at: 1132 1817 1132
Size: 685 3400
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 1510 1870 1510
Size: 360 3725
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SacII CC_GC'GG
Cuts at: 896 896
Size: 4085
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 1128 1813 2806 2997 33
Size: 1095 685 993 191 1121
Fragments arranged by size:
1121 1095 993 685 191
SfoI GGC'GCC
Cuts at: 354 1517 1765 354
Size: 1163 248 2674
Fragments arranged by size:
2674 1163 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 833 1107 833
Size: 274 3811
SmlI C'TyrA_G
Cuts at: 963 1240 2155 2647 2909 963
Size: 277 915 492 262 2139
Fragments arranged by size:
2139 915 492 277 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
StyI C'CwwG_G
Cuts at: 209 671 1219 1573 209
Size: 462 548 354 2721
Fragments arranged by size:
2721 548 462 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 783 1668 1801 2260 2469 783
Size: 885 133 459 209 2399
Fragments arranged by size:
2399 885 459 209 133
TatI w'GTAC_w
Cuts at: 3600 3600
Size: 4085
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 268 300 3602 3634 268
Size: 32 3302 32 719
Fragments arranged by size:
3302 719 32 32
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 1580 264
Size: 1316 2769
XhoI C'TCGA_G
Cuts at: 963 963
Size: 4085
XmaI C'CCGG_G
Cuts at: 831 1105 831
Size: 274 3811
XmnI GAAnn'nnTTC
Cuts at: 592 592
Size: 4085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspOMI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI