Restriction map for
pF1KB7983
(Circular) (Six-base) MAPSORT of: pF1KB7983.seq Check: 8348 from: 1 to: 4379
pF1KB7983 4379 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 237 237
Size: 4379
AccI GT'mk_AC
Cuts at: 992 1417 992
Size: 425 3954
Acc65I G'GTAC_C
Cuts at: 1395 1395
Size: 4379
AclI AA'CG_TT
Cuts at: 4219 4219
Size: 4379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 745 878 2204 2636 3383 3651 703
Size: 42 133 1326 432 747 268 1431
Fragments arranged by size:
1431 1326 747 432 268 133 42
AfeI AGC'GCT
Cuts at: 1089 1089
Size: 4379
AflIII A'CryG_T
Cuts at: 1731 2835 3812 1731
Size: 1104 977 2298
Fragments arranged by size:
2298 1104 977
AgeI A'CCGG_T
Cuts at: 1020 2767 1020
Size: 1747 2632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 792 824 792
Size: 32 4347
AlwNI CAG_nnn'CTG
Cuts at: 1684 3251 1684
Size: 1567 2812
ApaI G_GGCC'C
Cuts at: 219 219
Size: 4379
ApaLI G'TGCA_C
Cuts at: 3149 3149
Size: 4379
ApoI r'AATT_y
Cuts at: 239 272 1383 3732 3988 239
Size: 33 1111 2349 256 630
Fragments arranged by size:
2349 1111 630 256 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4379
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
AvaI C'yCGr_G
Cuts at: 824 1272 1399 824
Size: 448 127 3804
Fragments arranged by size:
3804 448 127
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4379
BanI G'GyrC_C
Cuts at: 577 609 1267 1276 1395 1809 2057 2092 577
Size: 32 658 9 119 414 248 35 2864
Fragments arranged by size:
2864 658 414 248 119 35 32 9
BanII G_rGCy'C
Cuts at: 219 1393 2423 219
Size: 1174 1030 2175
Fragments arranged by size:
2175 1174 1030
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4131
BbsI GAAGACnn'nnnn_
Cuts at: 939 939
Size: 4379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4345
BciVI GTATCCnnnnn_n'
Cuts at: 605 2277 3038 4379 605
Size: 1672 761 1341 605
Fragments arranged by size:
1672 1341 761 605
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4379
BglII A'GATC_T
Cuts at: 849 1894 3931 849
Size: 1045 2037 1297
Fragments arranged by size:
2037 1297 1045
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4379
Bme1580I G_kGCm'C
Cuts at: 219 391 580 1270 1279 2004 2097 3153 219
Size: 172 189 690 9 725 93 1056 1445
Fragments arranged by size:
1445 1056 725 690 189 172 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 366 1138 1591 1672 2007 4151 366
Size: 772 453 81 335 2144 594
Fragments arranged by size:
2144 772 594 453 335 81
BmtI G_CTAG'C
Cuts at: 205 503 205
Size: 298 4081
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 315 2746 315
Size: 2431 1948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 802 332
Size: 470 3909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2926 3224 1555
Size: 879 492 298 2710
Fragments arranged by size:
2710 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4083 4083
Size: 4379
BsaAI yAC'GTr
Cuts at: 1732 1732
Size: 4379
BsaBI GATnn'nnATC
Cuts at: 6 1893 3930 6
Size: 1887 2037 455
Fragments arranged by size:
2037 1887 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2760 4256 1810
Size: 248 702 1496 1933
Fragments arranged by size:
1933 1496 702 248
BsaWI w'CCGG_w
Cuts at: 565 1020 1568 2089 2767 3041 3188 3599 565
Size: 455 548 521 678 274 147 411 1345
Fragments arranged by size:
1345 678 548 521 455 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 775 805 376
Size: 30 369 30 3950
Fragments arranged by size:
3950 369 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1152 1152
Size: 4379
BseYI C'CCAG_C
Cuts at: 572 1105 1150 1804 2562 3139 3635 572
Size: 533 45 654 758 577 496 1316
Fragments arranged by size:
1316 758 654 577 533 496 45
BsiEI CG_ry'CG
Cuts at: 104 223 1921 1967 3175 104
Size: 119 1698 46 1208 1308
Fragments arranged by size:
1698 1308 1208 119 46
BsiHKAI G_wGCw'C
Cuts at: 1393 2171 2361 3153 1393
Size: 778 190 792 2619
Fragments arranged by size:
2619 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 219 391 580 1270 1279 1393 2004 2097 2171
Size: 172 189 690 9 114 611 93 74
Cuts at: 2171 2361 2423 3153 219
Size: 190 62 730 1445
Fragments arranged by size:
1445 730 690 611 190 189 172 114
93 74 62 9
BspEI T'CCGG_A
Cuts at: 3599 3599
Size: 4379
BspHI T'CATG_A
Cuts at: 3555 3555
Size: 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 272 1136 1429 1643 1945 2326 237
Size: 35 864 293 214 302 381 2290
Fragments arranged by size:
2290 864 381 302 293 214 35
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4209 4374 50
Size: 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2291 2291
Size: 4379
BsrFI r'CCGG_y
Cuts at: 1020 2377 2558 2767 1020
Size: 1357 181 209 2632
Fragments arranged by size:
2632 1357 209 181
BssSI C'ACGA_G
Cuts at: 2650 3008 2650
Size: 358 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1756 1756
Size: 4379
BstBI TT'CG_AA
Cuts at: 2740 2740
Size: 4379
BstYI r'GATC_y
Cuts at: 1 849 1404 1871 1894 2229 2475 3487 3573
Size: 848 555 467 23 335 246 1012 86
Cuts at: 3573 3585 3931 1
Size: 12 346 449
Fragments arranged by size:
1012 848 555 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1115 1115
Size: 4379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2329 2510 2673 2329
Size: 181 163 4035
Fragments arranged by size:
4035 181 163
BtsI GCAGTG_nn'
Cuts at: 166 1622 166
Size: 1456 2923
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4379
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2943 2085
Size: 858 3521
EaeI y'GGCC_r
Cuts at: 163 220 1360 1964 2138 2529 2556 3901 163
Size: 57 1140 604 174 391 27 1345 641
Fragments arranged by size:
1345 1140 641 604 391 174 57 27
EagI C'GGCC_G
Cuts at: 220 1964 220
Size: 1744 2635
EarI CTCTTCn'nnn_
Cuts at: 900 2402 2612 900
Size: 1502 210 2667
Fragments arranged by size:
2667 1502 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1221 2587 2897 3043 4189 1221
Size: 1366 310 146 1146 1411
Fragments arranged by size:
1411 1366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 703 745 802 878 2204 2636 3383 3651
Size: 371 42 57 76 1326 432 747 268
Cuts at: 3651 332
Size: 1060
Fragments arranged by size:
1326 1060 747 432 371 268 76 57
42
EcoO109I rG'GnC_Cy
Cuts at: 215 777 1518 215
Size: 562 741 3076
Fragments arranged by size:
3076 741 562
EcoRI G'AATT_C
Cuts at: 239 1383 239
Size: 1144 3235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4379
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4379
HaeII r_GCGC'y
Cuts at: 1091 1813 2061 3083 4062 1091
Size: 722 248 1022 979 1408
Fragments arranged by size:
1408 1022 979 722 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 2221 2251 2253 2283 376
Size: 32 1813 30 2 30 2472
Fragments arranged by size:
2472 1813 32 30 30 2
HincII GTy'rAC
Cuts at: 156 1418 156
Size: 1262 3117
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4131
KpnI G_GTAC'C
Cuts at: 1399 1399
Size: 4379
MluI A'CGCG_T
Cuts at: 3812 3812
Size: 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 707 3050 3234 278
Size: 429 2343 184 1423
Fragments arranged by size:
2343 1423 429 184
MscI TGG'CCA
Cuts at: 1362 2140 1362
Size: 778 3601
MslI CAynn'nnrTG
Cuts at: 2368 2495 2368
Size: 127 4252
MspA1I CmG'CkG
Cuts at: 125 212 723 1490 1804 2164 3177 3422 125
Size: 87 511 767 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 767 511 360 314 245 87
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4379
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4131
NcoI C'CATG_G
Cuts at: 1115 1115
Size: 4379
NdeI CA'TA_TG
Cuts at: 1235 1235
Size: 4379
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4379
NheI G'CTAG_C
Cuts at: 201 499 201
Size: 298 4081
NspI r_CATG'y
Cuts at: 534 1179 1432 1932 2839 534
Size: 645 253 500 907 2074
Fragments arranged by size:
2074 907 645 500 253
PasI CC'CwG_GG
Cuts at: 311 311
Size: 4379
PciI A'CATG_T
Cuts at: 2835 2835
Size: 4379
PfoI T'CCnGG_A
Cuts at: 1329 1329
Size: 4379
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 4347
PspOMI G'GGCC_C
Cuts at: 215 215
Size: 4379
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 741 773 741
Size: 32 4347
PstI C_TGCA'G
Cuts at: 248 1426 2111 248
Size: 1178 685 2516
Fragments arranged by size:
2516 1178 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4379
PvuII CAG'CTG
Cuts at: 723 1804 2164 723
Size: 1081 360 2938
Fragments arranged by size:
2938 1081 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4379
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4379
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4379
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4169
SbfI CC_TGCA'GG
Cuts at: 1426 1426
Size: 4379
ScaI AGT'ACT
Cuts at: 3896 3896
Size: 4379
SfcI C'TryA_G
Cuts at: 33 244 1083 1422 2107 3100 3291 33
Size: 211 839 339 685 993 191 1121
Fragments arranged by size:
1121 993 839 685 339 211 191
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4131
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4379
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4379
SmlI C'TyrA_G
Cuts at: 1534 2449 2941 3203 1534
Size: 915 492 262 2710
Fragments arranged by size:
2710 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4379
SphI G_CATG'C
Cuts at: 1179 1432 1932 1179
Size: 253 500 3626
Fragments arranged by size:
3626 500 253
StyI C'CwwG_G
Cuts at: 1115 1513 1867 1115
Size: 398 354 3627
Fragments arranged by size:
3627 398 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1962 2095 2554 2763 1962
Size: 133 459 209 3578
Fragments arranged by size:
3578 459 209 133
TatI w'GTAC_w
Cuts at: 3894 3894
Size: 4379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4347
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4379
XbaI T'CTAG_A
Cuts at: 1410 1410
Size: 4379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1113 1874 1113
Size: 761 3618
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4379
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PfoI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI Bpu10I BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI