Restriction map for
pF1KB7973
(Circular) (Six-base) MAPSORT of: pF1KB7973.seq Check: 6135 from: 1 to: 3755
pF1KB7973 3755 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 648 1580 2012 2759 3027 295
Size: 353 932 432 747 268 1023
Fragments arranged by size:
1023 932 747 432 353 268
AflIII A'CryG_T
Cuts at: 575 1107 2211 3188 575
Size: 532 1104 977 1142
Fragments arranged by size:
1142 1104 977 532
AgeI A'CCGG_T
Cuts at: 2143 2143
Size: 3755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 586 618 586
Size: 32 3723
AlwNI CAG_nnn'CTG
Cuts at: 648 699 1060 2627 648
Size: 51 361 1567 1776
Fragments arranged by size:
1776 1567 361 51
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 759 3108 3364 759
Size: 2349 256 1150
Fragments arranged by size:
2349 1150 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 130 775 130
Size: 645 3110
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 121 771 1185 1433 1468 121
Size: 650 414 248 35 2408
Fragments arranged by size:
2408 650 414 248 35
BanII G_rGCy'C
Cuts at: 769 1799 769
Size: 1030 2725
BbeI G_GCGC'C
Cuts at: 125 1189 1437 125
Size: 1064 248 2443
Fragments arranged by size:
2443 1064 248
BbsI GAAGACnn'nnnn_
Cuts at: 654 654
Size: 3755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 360 394 360
Size: 34 3721
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 266 367 867 266
Size: 101 500 3154
Fragments arranged by size:
3154 500 101
Bme1580I G_kGCm'C
Cuts at: 1380 1473 2529 1380
Size: 93 1056 2606
Fragments arranged by size:
2606 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 967 1048 1383 3527 967
Size: 81 335 2144 1195
Fragments arranged by size:
2144 1195 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1810 2302 2600 931
Size: 879 492 298 2086
Fragments arranged by size:
2086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 558 3459 558
Size: 2901 854
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 1269 3306 6
Size: 1263 2037 455
Fragments arranged by size:
2037 1263 455
BsaHI Gr'CG_yC
Cuts at: 122 284 719 1186 1434 2136 3632 122
Size: 162 435 467 248 702 1496 245
Fragments arranged by size:
1496 702 467 435 248 245 162
BsaWI w'CCGG_w
Cuts at: 944 1465 2143 2417 2564 2975 944
Size: 521 678 274 147 411 1724
Fragments arranged by size:
1724 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 433 463 433
Size: 30 3725
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 291 454 457 691 291
Size: 163 3 234 3355
Fragments arranged by size:
3355 234 163 3
BseYI C'CCAG_C
Cuts at: 1180 1938 2515 3011 1180
Size: 758 577 496 1924
Fragments arranged by size:
1924 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 709
Size: 3755
BsiEI CG_ry'CG
Cuts at: 104 1297 1343 2551 104
Size: 1193 46 1208 1308
Fragments arranged by size:
1308 1208 1193 46
BsiHKAI G_wGCw'C
Cuts at: 683 769 1547 1737 2529 683
Size: 86 778 190 792 1909
Fragments arranged by size:
1909 792 778 190 86
Bsp1286I G_dGCh'C
Cuts at: 683 769 1380 1473 1547 1737 1799 2529 683
Size: 86 611 93 74 190 62 730 1909
Fragments arranged by size:
1909 730 611 190 93 86 74 62
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 485 2931 485
Size: 2446 1309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 239 1753 1934 2143 239
Size: 1514 181 209 1851
Fragments arranged by size:
1851 1514 209 181
BssHII G'CGCG_C
Cuts at: 249 249
Size: 3755
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 1132 1132
Size: 3755
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstXI CCAn_nnnn'nTGG
Cuts at: 630 630
Size: 3755
BstYI r'GATC_y
Cuts at: 1 780 1247 1270 1605 1851 2863 2949 2961
Size: 779 467 23 335 246 1012 86 12
Cuts at: 2961 3307 1
Size: 346 449
Fragments arranged by size:
1012 779 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 998 998
Size: 3755
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 142 1461 2319 142
Size: 1319 858 1578
Fragments arranged by size:
1578 1319 858
EaeI y'GGCC_r
Cuts at: 632 1340 1514 1905 1932 3277 632
Size: 708 174 391 27 1345 1110
Fragments arranged by size:
1345 1110 708 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 268 1778 1988 268
Size: 1510 210 2035
Fragments arranged by size:
2035 1510 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1963 2273 2419 3565 1963
Size: 310 146 1146 2153
Fragments arranged by size:
2153 1146 310 146
EcoICRI GAG'CTC
Cuts at: 767 767
Size: 3755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 648 1580 2012 2759 3027 295
Size: 353 932 432 747 268 1023
Fragments arranged by size:
1023 932 747 432 353 268
EcoO109I rG'GnC_Cy
Cuts at: 135 894 135
Size: 759 2996
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3755
FspI TGC'GCA
Cuts at: 1536 1536
Size: 3755
HaeII r_GCGC'y
Cuts at: 125 1189 1437 2459 3438 125
Size: 1064 248 1022 979 442
Fragments arranged by size:
1064 1022 979 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 538 570 1597 1627 1629 1659 433
Size: 32 73 32 1027 30 2 30 2529
Fragments arranged by size:
2529 1027 73 32 32 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 121 1185 1433 121
Size: 1064 248 2443
Fragments arranged by size:
2443 1064 248
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MfeI C'AATT_G
Cuts at: 730 730
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 634 1516 634
Size: 882 2873
MslI CAynn'nnrTG
Cuts at: 490 628 1744 1871 490
Size: 138 1116 127 2374
Fragments arranged by size:
2374 1116 138 127
MspA1I CmG'CkG
Cuts at: 668 866 1180 1540 2553 2798 668
Size: 198 314 360 1013 245 1625
Fragments arranged by size:
1625 1013 360 314 245 198
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 122 1186 1434 122
Size: 1064 248 2443
Fragments arranged by size:
2443 1064 248
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NspI r_CATG'y
Cuts at: 808 1308 2215 808
Size: 500 907 2348
Fragments arranged by size:
2348 907 500
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PfoI T'CCnGG_A
Cuts at: 641 641
Size: 3755
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PpuMI rG'GwC_Cy
Cuts at: 135 135
Size: 3755
PshAI GACnn'nnGTC
Cuts at: 319 319
Size: 3755
PstI C_TGCA'G
Cuts at: 802 1487 802
Size: 685 3070
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 668 1180 1540 668
Size: 512 360 2883
Fragments arranged by size:
2883 512 360
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 769 769
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SexAI A'CCwGG_T
Cuts at: 548 560 548
Size: 12 3743
SfcI C'TryA_G
Cuts at: 33 162 798 1483 2476 2667 33
Size: 129 636 685 993 191 1121
Fragments arranged by size:
1121 993 685 636 191 129
SfoI GGC'GCC
Cuts at: 123 1187 1435 123
Size: 1064 248 2443
Fragments arranged by size:
2443 1064 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 910 1825 2317 2579 910
Size: 915 492 262 2086
Fragments arranged by size:
2086 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 808 1308 808
Size: 500 3255
StuI AGG'CCT
Cuts at: 217 217
Size: 3755
StyI C'CwwG_G
Cuts at: 139 889 1243 139
Size: 750 354 2651
Fragments arranged by size:
2651 750 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 3270 3270
Size: 3755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 550 562 1552 550
Size: 12 990 2753
Fragments arranged by size:
2753 990 12
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1250 1250
Size: 3755
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI