Restriction map for
pF1KB7961
(Circular) (Six-base) MAPSORT of: pF1KB7961.seq Check: 3511 from: 1 to: 3908
pF1KB7961 3908 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 946 946
Size: 3908
Acc65I G'GTAC_C
Cuts at: 924 924
Size: 3908
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 686 1733 2165 2912 3180 536
Size: 150 1047 432 747 268 1264
Fragments arranged by size:
1264 1047 747 432 268 150
AflIII A'CryG_T
Cuts at: 1260 2364 3341 1260
Size: 1104 977 1827
Fragments arranged by size:
1827 1104 977
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AleI CACnn'nnGTG
Cuts at: 882 882
Size: 3908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 201 184
Size: 17 3891
AlwNI CAG_nnn'CTG
Cuts at: 1213 2780 1213
Size: 1567 2341
ApaLI G'TGCA_C
Cuts at: 2678 2678
Size: 3908
ApoI r'AATT_y
Cuts at: 637 710 827 862 912 3261 3517 637
Size: 73 117 35 50 2349 256 1028
Fragments arranged by size:
2349 1028 256 117 73 50 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 928 928
Size: 3908
BamHI G'GATC_C
Cuts at: 933 933
Size: 3908
BanI G'GyrC_C
Cuts at: 924 1338 1586 1621 924
Size: 414 248 35 3211
Fragments arranged by size:
3211 414 248 35
BanII G_rGCy'C
Cuts at: 804 922 1952 804
Size: 118 1030 2760
Fragments arranged by size:
2760 1030 118
BbeI G_GCGC'C
Cuts at: 1342 1590 1342
Size: 248 3660
BbsI GAAGACnn'nnnn_
Cuts at: 899 899
Size: 3908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3874
BciVI GTATCCnnnnn_n'
Cuts at: 1806 2567 3908 1806
Size: 761 1341 1806
Fragments arranged by size:
1806 1341 761
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BfrBI ATG'CAT
Cuts at: 178 178
Size: 3908
BglI GCCn_nnn'nGGC
Cuts at: 449 449
Size: 3908
BglII A'GATC_T
Cuts at: 1423 3460 1423
Size: 2037 1871
BlpI GC'TnA_GC
Cuts at: 1020 1020
Size: 3908
Bme1580I G_kGCm'C
Cuts at: 1533 1626 2682 1533
Size: 93 1056 2759
Fragments arranged by size:
2759 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1120 1201 1536 3680 1120
Size: 81 335 2144 1348
Fragments arranged by size:
2144 1348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 3908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 457 310
Size: 147 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1963 2455 2753 1084
Size: 879 492 298 2239
Fragments arranged by size:
2239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 641 3612 641
Size: 2971 937
BsaAI yAC'GTr
Cuts at: 1261 1261
Size: 3908
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 1339 1587 2289 3785 1339
Size: 248 702 1496 1462
Fragments arranged by size:
1496 1462 702 248
BsaWI w'CCGG_w
Cuts at: 1097 1618 2296 2570 2717 3128 1097
Size: 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 436
Size: 3908
BseYI C'CCAG_C
Cuts at: 1333 2091 2668 3164 1333
Size: 758 577 496 2077
Fragments arranged by size:
2077 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 426
Size: 3908
BsiEI CG_ry'CG
Cuts at: 104 1450 1496 2704 104
Size: 1346 46 1208 1308
Fragments arranged by size:
1346 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 922 1700 1890 2682 922
Size: 778 190 792 2148
Fragments arranged by size:
2148 792 778 190
BsmI GAATG_Cn'
Cuts at: 680 680
Size: 3908
Bsp1286I G_dGCh'C
Cuts at: 804 922 1533 1626 1700 1890 1952 2682 804
Size: 118 611 93 74 190 62 730 2030
Fragments arranged by size:
2030 730 611 190 118 93 74 62
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 3084 3084
Size: 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 659 958 1172 1474 1855 659
Size: 299 214 302 381 2712
Fragments arranged by size:
2712 381 302 299 214
BsrBI CCG'CTC
Cuts at: 50 837 2200 2254 3738 3903 50
Size: 787 1363 54 1484 165 55
Fragments arranged by size:
1484 1363 787 165 55 54
BsrDI GCAATG_nn'
Cuts at: 228 1820 228
Size: 1592 2316
BsrFI r'CCGG_y
Cuts at: 1906 2087 2296 1906
Size: 181 209 3518
Fragments arranged by size:
3518 209 181
BsrGI T'GTAC_A
Cuts at: 857 857
Size: 3908
BssSI C'ACGA_G
Cuts at: 2179 2537 2179
Size: 358 3550
BstAPI GCAn_nnn'nTGC
Cuts at: 1285 1285
Size: 3908
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstYI r'GATC_y
Cuts at: 1 933 1400 1423 1758 2004 3016 3102 3114
Size: 932 467 23 335 246 1012 86 12
Cuts at: 3114 3460 1
Size: 346 449
Fragments arranged by size:
1012 932 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1858 2039 2202 1858
Size: 181 163 3564
Fragments arranged by size:
3564 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1151
Size: 3908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 372 407 372
Size: 35 3873
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 1614 2472 1614
Size: 858 3050
EaeI y'GGCC_r
Cuts at: 1493 1667 2058 2085 3430 1493
Size: 174 391 27 1345 1971
Fragments arranged by size:
1971 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1493 1493
Size: 3908
EarI CTCTTCn'nnn_
Cuts at: 221 1931 2141 221
Size: 1710 210 1988
Fragments arranged by size:
1988 1710 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2116 2426 2572 3718 2116
Size: 310 146 1146 2306
Fragments arranged by size:
2306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 457 536 686 1733 2165 2912 3180 310
Size: 147 79 150 1047 432 747 268 1038
Fragments arranged by size:
1047 1038 747 432 268 150 147 79
EcoO109I rG'GnC_Cy
Cuts at: 1047 1047
Size: 3908
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 309 1342 1590 2612 3591 309
Size: 1033 248 1022 979 626
Fragments arranged by size:
1033 1022 979 626 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1750 1780 1782 1812 1750
Size: 30 2 30 3846
Fragments arranged by size:
3846 30 30 2
HincII GTy'rAC
Cuts at: 843 947 843
Size: 104 3804
KasI G'GCGC_C
Cuts at: 1338 1586 1338
Size: 248 3660
KpnI G_GTAC'C
Cuts at: 928 928
Size: 3908
MfeI C'AATT_G
Cuts at: 360 360
Size: 3908
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 122 332 578 2579 2763 122
Size: 210 246 2001 184 1267
Fragments arranged by size:
2001 1267 246 210 184
MscI TGG'CCA
Cuts at: 1669 1669
Size: 3908
MslI CAynn'nnrTG
Cuts at: 882 1897 2024 882
Size: 1015 127 2766
Fragments arranged by size:
2766 1015 127
MspA1I CmG'CkG
Cuts at: 402 439 1019 1333 1693 2706 2951 402
Size: 37 580 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 580 360 314 245 37
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 3908
NarI GG'CG_CC
Cuts at: 1339 1587 1339
Size: 248 3660
NdeI CA'TA_TG
Cuts at: 783 783
Size: 3908
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 3908
NsiI A_TGCA'T
Cuts at: 180 180
Size: 3908
NspI r_CATG'y
Cuts at: 961 1461 2368 961
Size: 500 907 2501
Fragments arranged by size:
2501 907 500
PciI A'CATG_T
Cuts at: 2364 2364
Size: 3908
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3876
PshAI GACnn'nnGTC
Cuts at: 280 280
Size: 3908
PstI C_TGCA'G
Cuts at: 401 955 1640 401
Size: 554 685 2669
Fragments arranged by size:
2669 685 554
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 402 439 1333 1693 402
Size: 37 894 360 2617
Fragments arranged by size:
2617 894 360 37
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 3908
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
SbfI CC_TGCA'GG
Cuts at: 955 955
Size: 3908
ScaI AGT'ACT
Cuts at: 232 3425 232
Size: 3193 715
SfcI C'TryA_G
Cuts at: 33 397 951 1636 2629 2820 33
Size: 364 554 685 993 191 1121
Fragments arranged by size:
1121 993 685 554 364 191
SfoI GGC'GCC
Cuts at: 1340 1588 1340
Size: 248 3660
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 930 930
Size: 3908
SmlI C'TyrA_G
Cuts at: 1063 1978 2470 2732 1063
Size: 915 492 262 2239
Fragments arranged by size:
2239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 961 1461 961
Size: 500 3408
StyI C'CwwG_G
Cuts at: 1042 1396 1042
Size: 354 3554
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 860 1491 1624 2083 2292 860
Size: 631 133 459 209 2476
Fragments arranged by size:
2476 631 459 209 133
TatI w'GTAC_w
Cuts at: 230 344 461 857 3423 230
Size: 114 117 396 2566 715
Fragments arranged by size:
2566 715 396 117 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 3876
Tth111I GACn'n_nGTC
Cuts at: 1705 1705
Size: 3908
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1403 1403
Size: 3908
XmaI C'CCGG_G
Cuts at: 928 928
Size: 3908
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI