Restriction map for
pF1KB7948
(Circular) (Six-base) MAPSORT of: pF1KB7948.seq Check: 9397 from: 1 to: 4238
pF1KB7948 4238 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 648 648
Size: 4238
AccI GT'mk_AC
Cuts at: 1070 1276 1070
Size: 206 4032
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 2063 2495 3242 3510 169
Size: 1894 432 747 268 897
Fragments arranged by size:
1894 897 747 432 268
AflIII A'CryG_T
Cuts at: 909 1590 2694 3671 909
Size: 681 1104 977 1476
Fragments arranged by size:
1476 1104 977 681
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 295 1543 3110 295
Size: 1248 1567 1423
Fragments arranged by size:
1567 1423 1248
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 268 323 1242 3591 3847 268
Size: 55 919 2349 256 659
Fragments arranged by size:
2349 919 659 256 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 485 1258 485
Size: 773 3465
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1254 1668 1916 1951 1254
Size: 414 248 35 3541
Fragments arranged by size:
3541 414 248 35
BanII G_rGCy'C
Cuts at: 1252 2282 1252
Size: 1030 3208
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbsI GAAGACnn'nnnn_
Cuts at: 122 122
Size: 4238
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 1148 1350 1148
Size: 202 4036
Bme1580I G_kGCm'C
Cuts at: 1863 1956 3012 1863
Size: 93 1056 3089
Fragments arranged by size:
3089 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 360 1450 1531 1866 4010 360
Size: 1090 81 335 2144 588
Fragments arranged by size:
2144 1090 588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3942 3942
Size: 4238
BsaAI yAC'GTr
Cuts at: 144 1591 144
Size: 1447 2791
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1160 1178 1160
Size: 18 4220
BseYI C'CCAG_C
Cuts at: 1663 2421 2998 3494 1663
Size: 758 577 496 2407
Fragments arranged by size:
2407 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 884 626
Size: 258 3980
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1149 1252 2030 2220 3012 1149
Size: 103 778 190 792 2375
Fragments arranged by size:
2375 792 778 190 103
Bsp1286I G_dGCh'C
Cuts at: 1149 1252 1863 1956 2030 2220 2282 3012 1149
Size: 103 611 93 74 190 62 730 2375
Fragments arranged by size:
2375 730 611 190 103 93 74 62
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 648 1288 1502 1804 2185 648
Size: 640 214 302 381 2701
Fragments arranged by size:
2701 640 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 2150 252
Size: 1898 2340
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 295 304 565 871 1615 295
Size: 9 261 306 744 2918
Fragments arranged by size:
2918 744 306 261 9
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstEII G'GTnAC_C
Cuts at: 148 148
Size: 4238
BstXI CCAn_nnnn'nTGG
Cuts at: 1006 1006
Size: 4238
BstYI r'GATC_y
Cuts at: 1 1263 1730 1753 2088 2334 3346 3432 3444
Size: 1262 467 23 335 246 1012 86 12
Cuts at: 3444 3790 1
Size: 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1071 1071
Size: 4238
BtgI C'CryG_G
Cuts at: 1081 1081
Size: 4238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 1481
Size: 4238
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 1823 1997 2388 2415 3760 1823
Size: 174 391 27 1345 2301
Fragments arranged by size:
2301 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 2063 2495 3242 3510 169
Size: 1894 432 747 268 897
Fragments arranged by size:
1894 897 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1157 1157
Size: 4238
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
EcoRV GAT'ATC
Cuts at: 444 444
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1672 1920 2942 3921 1672
Size: 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 2080 2110 2112 2142 433
Size: 32 1615 30 2 30 2529
Fragments arranged by size:
2529 1615 32 30 30 2
HincII GTy'rAC
Cuts at: 755 972 1277 755
Size: 217 305 3716
Fragments arranged by size:
3716 305 217
HpaI GTT'AAC
Cuts at: 755 755
Size: 4238
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MfeI C'AATT_G
Cuts at: 618 618
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 987 2909 3093 987
Size: 1922 184 2132
Fragments arranged by size:
2132 1922 184
MscI TGG'CCA
Cuts at: 1999 1999
Size: 4238
MslI CAynn'nnrTG
Cuts at: 284 398 860 2227 2354 284
Size: 114 462 1367 127 2168
Fragments arranged by size:
2168 1367 462 127 114
MspA1I CmG'CkG
Cuts at: 292 1349 1663 2023 3036 3281 292
Size: 1057 314 360 1013 245 1249
Fragments arranged by size:
1249 1057 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NcoI C'CATG_G
Cuts at: 1081 1081
Size: 4238
NdeI CA'TA_TG
Cuts at: 578 578
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 913 1291 1791 2698 913
Size: 378 500 907 2453
Fragments arranged by size:
2453 907 500 378
PciI A'CATG_T
Cuts at: 909 2694 909
Size: 1785 2453
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PshAI GACnn'nnGTC
Cuts at: 172 977 172
Size: 805 3433
PsiI TTA'TAA
Cuts at: 545 545
Size: 4238
PstI C_TGCA'G
Cuts at: 1285 1970 1285
Size: 685 3553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 292 1663 2023 292
Size: 1371 360 2507
Fragments arranged by size:
2507 1371 360
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 975 1281 1966 2959 3150 33
Size: 942 306 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 306 191
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 487 1260 487
Size: 773 3465
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SnaBI TAC'GTA
Cuts at: 144 144
Size: 4238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StyI C'CwwG_G
Cuts at: 1081 1158 1372 1726 1081
Size: 77 214 354 3593
Fragments arranged by size:
3593 354 214 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 586 1034 1123 1821 1954 2413 2622 586
Size: 448 89 698 133 459 209 2202
Fragments arranged by size:
2202 698 459 448 209 133 89
TatI w'GTAC_w
Cuts at: 652 721 728 3753 652
Size: 69 7 3025 1137
Fragments arranged by size:
3025 1137 69 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 406 1204 1733 406
Size: 798 529 2911
Fragments arranged by size:
2911 798 529
XmaI C'CCGG_G
Cuts at: 485 1258 485
Size: 773 3465
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI XmnI
ZraI