Restriction map for
pF1KB7945
(Circular) (Six-base) MAPSORT of: pF1KB7945.seq Check: 8026 from: 1 to: 4286
pF1KB7945 4286 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 594 1260 594
Size: 666 3620
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1049 1302 1049
Size: 253 4033
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2543 3290 3558 2111
Size: 432 747 268 2839
Fragments arranged by size:
2839 747 432 268
AfeI AGC'GCT
Cuts at: 1227 1227
Size: 4286
AflIII A'CryG_T
Cuts at: 1059 1638 2742 3719 1059
Size: 579 1104 977 1626
Fragments arranged by size:
1626 1104 977 579
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 260 807 1591 3158 260
Size: 547 784 1567 1388
Fragments arranged by size:
1567 1388 784 547
ApaLI G'TGCA_C
Cuts at: 3056 3056
Size: 4286
ApoI r'AATT_y
Cuts at: 308 1290 3639 3895 308
Size: 982 2349 256 699
Fragments arranged by size:
2349 982 699 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 880 1045 1194 1235 1306 880
Size: 165 149 41 71 3860
Fragments arranged by size:
3860 165 149 71 41
BamHI G'GATC_C
Cuts at: 361 1311 361
Size: 950 3336
BanI G'GyrC_C
Cuts at: 729 1049 1113 1302 1716 1964 1999 729
Size: 320 64 189 414 248 35 3016
Fragments arranged by size:
3016 414 320 248 189 64 35
BanII G_rGCy'C
Cuts at: 872 1300 2330 872
Size: 428 1030 2828
Fragments arranged by size:
2828 1030 428
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BciVI GTATCCnnnnn_n'
Cuts at: 2184 2945 4286 2184
Size: 761 1341 2184
Fragments arranged by size:
2184 1341 761
BclI T'GATC_A
Cuts at: 1806 1806
Size: 4286
BglI GCCn_nnn'nGGC
Cuts at: 916 949 916
Size: 33 4253
BglII A'GATC_T
Cuts at: 1801 3838 1801
Size: 2037 2249
BlpI GC'TnA_GC
Cuts at: 1398 1398
Size: 4286
Bme1580I G_kGCm'C
Cuts at: 1911 2004 3060 1911
Size: 93 1056 3137
Fragments arranged by size:
3137 1056 93
BmgBI CAC'GTC
Cuts at: 671 1062 671
Size: 391 3895
BmrI ACTGGGnnnn_n'
Cuts at: 1498 1579 1914 4058 1498
Size: 81 335 2144 1726
Fragments arranged by size:
2144 1726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 781 2653 781
Size: 1872 2414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 829 1200 255
Size: 574 371 3341
Fragments arranged by size:
3341 574 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2341 2833 3131 1462
Size: 879 492 298 2617
Fragments arranged by size:
2617 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3990 3990
Size: 4286
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 1800 3837 6
Size: 1794 2037 455
Fragments arranged by size:
2037 1794 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 524 726 767 1475 1996 2674 2948 3095 3506
Size: 202 41 708 521 678 274 147 411
Cuts at: 3506 524
Size: 1304
Fragments arranged by size:
1304 708 678 521 411 274 202 147
41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 188 218 1159 1189 188
Size: 30 941 30 3285
Fragments arranged by size:
3285 941 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 125 176 1253 125
Size: 51 1077 3158
Fragments arranged by size:
3158 1077 51
BseYI C'CCAG_C
Cuts at: 561 676 1711 2469 3046 3542 561
Size: 115 1035 758 577 496 1305
Fragments arranged by size:
1305 1035 758 577 496 115
BsiEI CG_ry'CG
Cuts at: 104 632 1828 1874 3082 104
Size: 528 1196 46 1208 1308
Fragments arranged by size:
1308 1208 1196 528 46
BsiHKAI G_wGCw'C
Cuts at: 622 1036 1300 2078 2268 3060 622
Size: 414 264 778 190 792 1848
Fragments arranged by size:
1848 792 778 414 264 190
BsmBI CGTCTCn'nnnn_
Cuts at: 698 698
Size: 4286
Bsp1286I G_dGCh'C
Cuts at: 622 872 1036 1300 1911 2004 2078 2268 2330
Size: 250 164 264 611 93 74 190 62
Cuts at: 2330 3060 622
Size: 730 1848
Fragments arranged by size:
1848 730 611 264 250 190 164 93
74 62
BspEI T'CCGG_A
Cuts at: 524 767 3506 524
Size: 243 2739 1304
Fragments arranged by size:
2739 1304 243
BspHI T'CATG_A
Cuts at: 3462 3462
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 308 594 1260 1336 1550 1852 2233 308
Size: 286 666 76 214 302 381 2361
Fragments arranged by size:
2361 666 381 302 286 214 76
BsrBI CCG'CTC
Cuts at: 50 471 2578 2632 4116 4281 50
Size: 421 2107 54 1484 165 55
Fragments arranged by size:
2107 1484 421 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 455 631 2284 2465 2674 455
Size: 176 1653 181 209 2067
Fragments arranged by size:
2067 1653 209 181 176
BssSI C'ACGA_G
Cuts at: 2557 2915 2557
Size: 358 3928
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1663
Size: 4286
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 393 393
Size: 4286
BstYI r'GATC_y
Cuts at: 1 361 1311 1778 1801 2136 2382 3394 3480
Size: 360 950 467 23 335 246 1012 86
Cuts at: 3480 3492 3838 1
Size: 12 346 449
Fragments arranged by size:
1012 950 467 449 360 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 291 905 107
Size: 184 614 3488
Fragments arranged by size:
3488 614 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2236 2417 2580 2236
Size: 181 163 3942
Fragments arranged by size:
3942 181 163
BtsI GCAGTG_nn'
Cuts at: 987 1262 1529 987
Size: 275 267 3744
Fragments arranged by size:
3744 275 267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 417 452 417
Size: 35 4251
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 606 1992 2850 606
Size: 1386 858 2042
Fragments arranged by size:
2042 1386 858
EaeI y'GGCC_r
Cuts at: 199 629 738 950 1871 2045 2436 2463 3808
Size: 430 109 212 921 174 391 27 1345
Cuts at: 3808 199
Size: 677
Fragments arranged by size:
1345 921 677 430 391 212 174 109
27
EagI C'GGCC_G
Cuts at: 629 1871 629
Size: 1242 3044
EarI CTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 208 2494 2804 2950 4096 208
Size: 2286 310 146 1146 398
Fragments arranged by size:
2286 1146 398 310 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 829 1200 2111 2543 3290 3558 255
Size: 574 371 911 432 747 268 983
Fragments arranged by size:
983 911 747 574 432 371 268
EcoO109I rG'GnC_Cy
Cuts at: 257 1425 257
Size: 1168 3118
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 4286
FspI TGC'GCA
Cuts at: 2067 2067
Size: 4286
HaeII r_GCGC'y
Cuts at: 683 1229 1720 1968 2990 3969 683
Size: 546 491 248 1022 979 1000
Fragments arranged by size:
1022 1000 979 546 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 220 2128 2158 2160 2190 188
Size: 32 1908 30 2 30 2284
Fragments arranged by size:
2284 1908 32 30 30 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1053 1306 1053
Size: 253 4033
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 154 2957 3141 154
Size: 2803 184 1299
Fragments arranged by size:
2803 1299 184
MscI TGG'CCA
Cuts at: 952 2047 952
Size: 1095 3191
MslI CAynn'nnrTG
Cuts at: 248 296 2275 2402 248
Size: 48 1979 127 2132
Fragments arranged by size:
2132 1979 127 48
MspA1I CmG'CkG
Cuts at: 653 807 1072 1397 1711 2071 3084 3329 653
Size: 154 265 325 314 360 1013 245 1610
Fragments arranged by size:
1610 1013 360 325 314 265 245 154
NaeI GCC'GGC
Cuts at: 633 2467 633
Size: 1834 2452
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 107 291 905 107
Size: 184 614 3488
Fragments arranged by size:
3488 614 184
NgoMIV G'CCGG_C
Cuts at: 631 2465 631
Size: 1834 2452
NspI r_CATG'y
Cuts at: 1339 1839 2746 1339
Size: 500 907 2879
Fragments arranged by size:
2879 907 500
PciI A'CATG_T
Cuts at: 2742 2742
Size: 4286
PfoI T'CCnGG_A
Cuts at: 206 1132 1177 206
Size: 926 45 3315
Fragments arranged by size:
3315 926 45
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 727 759 3425 3457 727
Size: 32 2666 32 1556
Fragments arranged by size:
2666 1556 32 32
PstI C_TGCA'G
Cuts at: 1257 1333 2018 1257
Size: 76 685 3525
Fragments arranged by size:
3525 685 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 807 1711 2071 807
Size: 904 360 3022
Fragments arranged by size:
3022 904 360
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 1143 1253 1329 2014 3007 3198 33
Size: 1110 110 76 685 993 191 1121
Fragments arranged by size:
1121 1110 993 685 191 110 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 949 949
Size: 4286
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1047 1196 1308 1047
Size: 149 112 4025
Fragments arranged by size:
4025 149 112
SmlI C'TyrA_G
Cuts at: 1441 2356 2848 3110 1441
Size: 915 492 262 2617
Fragments arranged by size:
2617 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
StyI C'CwwG_G
Cuts at: 107 291 905 1420 1774 107
Size: 184 614 515 354 2619
Fragments arranged by size:
2619 614 515 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 399 561 1869 2002 2461 2670 399
Size: 162 1308 133 459 209 2015
Fragments arranged by size:
2015 1308 459 209 162 133
TatI w'GTAC_w
Cuts at: 534 3801 534
Size: 3267 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4254
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 912 1781 387
Size: 525 869 2892
Fragments arranged by size:
2892 869 525
XmaI C'CCGG_G
Cuts at: 1045 1194 1306 1045
Size: 149 112 4025
Fragments arranged by size:
4025 149 112
XmnI GAAnn'nnTTC
Cuts at: 713 713
Size: 4286
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI