Restriction map for
pF1KB7932
(Circular) (Six-base) MAPSORT of: pF1KB7932.seq Check: 4002 from: 1 to: 3752
pF1KB7932 3752 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 574 790 574
Size: 216 3536
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 447 1577 2009 2756 3024 203
Size: 244 1130 432 747 268 931
Fragments arranged by size:
1130 931 747 432 268 244
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 1057 2624 1057
Size: 1567 2185
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 772 772
Size: 3752
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 208 253 768 1182 1430 1465 208
Size: 45 515 414 248 35 2495
Fragments arranged by size:
2495 515 414 248 45 35
BanII G_rGCy'C
Cuts at: 320 766 1796 320
Size: 446 1030 2276
Fragments arranged by size:
2276 1030 446
BbeI G_GCGC'C
Cuts at: 212 257 1186 1434 212
Size: 45 929 248 2530
Fragments arranged by size:
2530 929 248 45
BbsI GAAGACnn'nnnn_
Cuts at: 194 680 194
Size: 486 3266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3718
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglI GCCn_nnn'nGGC
Cuts at: 369 369
Size: 3752
BglII A'GATC_T
Cuts at: 666 1267 3304 666
Size: 601 2037 1114
Fragments arranged by size:
2037 1114 601
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 1377 1470 2526 1377
Size: 93 1056 2603
Fragments arranged by size:
2603 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 138 259 964 1045 1380 3524 138
Size: 121 705 81 335 2144 366
Fragments arranged by size:
2144 705 366 335 121 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 721 580
Size: 141 3611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1807 2299 2597 928
Size: 879 492 298 2083
Fragments arranged by size:
2083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 164 209 254 1183 1431 2133 3629 164
Size: 45 45 929 248 702 1496 287
Fragments arranged by size:
1496 929 702 287 248 45 45
BsaWI w'CCGG_w
Cuts at: 605 941 1462 2140 2414 2561 2972 605
Size: 336 521 678 274 147 411 1385
Fragments arranged by size:
1385 678 521 411 336 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 214 229 274 277 169
Size: 45 15 45 3 3644
Fragments arranged by size:
3644 45 45 15 3
BseYI C'CCAG_C
Cuts at: 1177 1935 2512 3008 1177
Size: 758 577 496 1921
Fragments arranged by size:
1921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 709
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 283 1294 1340 2548 104
Size: 179 1011 46 1208 1308
Fragments arranged by size:
1308 1208 1011 179 46
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 320 766 1377 1470 1544 1734 1796 2526 320
Size: 446 611 93 74 190 62 730 1546
Fragments arranged by size:
1546 730 611 446 190 93 74 62
BspEI T'CCGG_A
Cuts at: 605 2972 605
Size: 2367 1385
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 705 2044 2098 3582 3747 50
Size: 655 1339 54 1484 165 55
Fragments arranged by size:
1484 1339 655 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 1664 722
Size: 942 2810
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstEII G'GTnAC_C
Cuts at: 448 448
Size: 3752
BstYI r'GATC_y
Cuts at: 1 666 777 1244 1267 1602 1848 2860 2946
Size: 665 111 467 23 335 246 1012 86
Cuts at: 2946 2958 3304 1
Size: 12 346 449
Fragments arranged by size:
1012 665 467 449 346 335 246 111
86 23 12
BstZ17I GTA'TAC
Cuts at: 575 575
Size: 3752
BtgI C'CryG_G
Cuts at: 283 283
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 603 1702 1883 2046 603
Size: 1099 181 163 2309
Fragments arranged by size:
2309 1099 181 163
BtsI GCAGTG_nn'
Cuts at: 562 995 562
Size: 433 3319
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 280 1337 1511 1902 1929 3274 280
Size: 1057 174 391 27 1345 758
Fragments arranged by size:
1345 1057 758 391 174 27
EagI C'GGCC_G
Cuts at: 280 1337 280
Size: 1057 2695
EarI CTCTTCn'nnn_
Cuts at: 164 224 466 1775 1985 164
Size: 60 242 1309 210 1931
Fragments arranged by size:
1931 1309 242 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 1960 2270 2416 3562 136
Size: 1824 310 146 1146 326
Fragments arranged by size:
1824 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 447 580 721 1577 2009 2756 3024 203
Size: 244 133 141 856 432 747 268 931
Fragments arranged by size:
931 856 747 432 268 244 141 133
EcoO109I rG'GnC_Cy
Cuts at: 891 891
Size: 3752
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 212 257 1186 1434 2456 3435 212
Size: 45 929 248 1022 979 529
Fragments arranged by size:
1022 979 929 529 248 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 629 661 1594 1624 1626 1656 629
Size: 32 933 30 2 30 2725
Fragments arranged by size:
2725 933 32 30 30 2
HincII GTy'rAC
Cuts at: 498 534 791 498
Size: 36 257 3459
Fragments arranged by size:
3459 257 36
HpaI GTT'AAC
Cuts at: 498 498
Size: 3752
KasI G'GCGC_C
Cuts at: 208 253 1182 1430 208
Size: 45 929 248 2530
Fragments arranged by size:
2530 929 248 45
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 683 1741 1868 683
Size: 1058 127 2567
Fragments arranged by size:
2567 1058 127
MspA1I CmG'CkG
Cuts at: 285 863 1177 1537 2550 2795 285
Size: 578 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 578 360 314 245
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 209 254 1183 1431 209
Size: 45 929 248 2530
Fragments arranged by size:
2530 929 248 45
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NotI GC'GGCC_GC
Cuts at: 280 280
Size: 3752
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SacII CC_GC'GG
Cuts at: 286 286
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 466 1775 1985 466
Size: 1309 210 2233
Fragments arranged by size:
2233 1309 210
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 210 255 1184 1432 210
Size: 45 929 248 2530
Fragments arranged by size:
2530 929 248 45
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 907 1822 2314 2576 907
Size: 915 492 262 2083
Fragments arranged by size:
2083 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 886 1240 886
Size: 354 3398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 695 1335 1468 1927 2136 695
Size: 640 133 459 209 2311
Fragments arranged by size:
2311 640 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 646 1549 646
Size: 903 2849
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NcoI NdeI NheI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI