Restriction map for
pF1KB7925
(Circular) (Six-base) MAPSORT of: pF1KB7925.seq Check: 1031 from: 1 to: 3689
pF1KB7925 3689 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 727 727
Size: 3689
Acc65I G'GTAC_C
Cuts at: 705 705
Size: 3689
AclI AA'CG_TT
Cuts at: 3529 3529
Size: 3689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1514 1946 2693 2961 1514
Size: 432 747 268 2242
Fragments arranged by size:
2242 747 432 268
AflIII A'CryG_T
Cuts at: 1041 2145 3122 1041
Size: 1104 977 1608
Fragments arranged by size:
1608 1104 977
AgeI A'CCGG_T
Cuts at: 2077 2077
Size: 3689
AlwNI CAG_nnn'CTG
Cuts at: 315 577 994 2561 315
Size: 262 417 1567 1443
Fragments arranged by size:
1567 1443 417 262
ApaI G_GGCC'C
Cuts at: 348 348
Size: 3689
ApaLI G'TGCA_C
Cuts at: 2459 2459
Size: 3689
ApoI r'AATT_y
Cuts at: 144 693 3042 3298 144
Size: 549 2349 256 535
Fragments arranged by size:
2349 549 535 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 199 223 709 199
Size: 24 486 3179
Fragments arranged by size:
3179 486 24
BamHI G'GATC_C
Cuts at: 333 714 333
Size: 381 3308
BanI G'GyrC_C
Cuts at: 705 1119 1367 1402 705
Size: 414 248 35 2992
Fragments arranged by size:
2992 414 248 35
BanII G_rGCy'C
Cuts at: 168 238 249 348 703 1733 168
Size: 70 11 99 355 1030 2124
Fragments arranged by size:
2124 1030 355 99 70 11
BbeI G_GCGC'C
Cuts at: 1123 1371 1123
Size: 248 3441
BbvCI CC'TCA_GC
Cuts at: 494 494
Size: 3689
BciVI GTATCCnnnnn_n'
Cuts at: 1587 2348 3689 1587
Size: 761 1341 1587
Fragments arranged by size:
1587 1341 761
BclI T'GATC_A
Cuts at: 1209 1209
Size: 3689
BfrBI ATG'CAT
Cuts at: 668 668
Size: 3689
BglI GCCn_nnn'nGGC
Cuts at: 496 496
Size: 3689
BglII A'GATC_T
Cuts at: 1204 3241 1204
Size: 2037 1652
BlpI GC'TnA_GC
Cuts at: 801 801
Size: 3689
Bme1580I G_kGCm'C
Cuts at: 348 1314 1407 2463 348
Size: 966 93 1056 1574
Fragments arranged by size:
1574 1056 966 93
BmrI ACTGGGnnnn_n'
Cuts at: 182 901 982 1317 3461 182
Size: 719 81 335 2144 410
Fragments arranged by size:
2144 719 410 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 2056 252
Size: 1804 1885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 367 505 602 278
Size: 89 138 97 3365
Fragments arranged by size:
3365 138 97 89
Bpu10I CC'TnA_GC
Cuts at: 494 494
Size: 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 699 865 1744 2236 2534 699
Size: 166 879 492 298 1854
Fragments arranged by size:
1854 879 492 298 166
BsaI GGTCTCn'nnnn_
Cuts at: 196 3393 196
Size: 3197 492
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 1203 3240 6
Size: 1197 2037 455
Fragments arranged by size:
2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 1120 1368 2070 3566 1120
Size: 248 702 1496 1243
Fragments arranged by size:
1496 1243 702 248
BsaWI w'CCGG_w
Cuts at: 878 1399 2077 2351 2498 2909 878
Size: 521 678 274 147 411 1658
Fragments arranged by size:
1658 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 529
Size: 30 3659
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 241
Size: 3689
BseYI C'CCAG_C
Cuts at: 358 604 1114 1872 2449 2945 358
Size: 246 510 758 577 496 1102
Fragments arranged by size:
1102 758 577 510 496 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3689
BsiEI CG_ry'CG
Cuts at: 104 1231 1277 2485 104
Size: 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 46
BsiHKAI G_wGCw'C
Cuts at: 238 703 1481 1671 2463 238
Size: 465 778 190 792 1464
Fragments arranged by size:
1464 792 778 465 190
Bsp1286I G_dGCh'C
Cuts at: 168 238 249 348 703 1314 1407 1481 1671
Size: 70 11 99 355 611 93 74 190
Cuts at: 1671 1733 2463 168
Size: 62 730 1394
Fragments arranged by size:
1394 730 611 355 190 99 93 74
70 62 11
BspEI T'CCGG_A
Cuts at: 2909 2909
Size: 3689
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 953 1255 1636 739
Size: 214 302 381 2792
Fragments arranged by size:
2792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1981 2035 3519 3684 50
Size: 1931 54 1484 165 55
Fragments arranged by size:
1931 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1601 1601
Size: 3689
BsrFI r'CCGG_y
Cuts at: 1687 1868 2077 1687
Size: 181 209 3299
Fragments arranged by size:
3299 209 181
BssSI C'ACGA_G
Cuts at: 1960 2318 1960
Size: 358 3331
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1066
Size: 3689
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstYI r'GATC_y
Cuts at: 1 333 714 1181 1204 1539 1785 2797 2883
Size: 332 381 467 23 335 246 1012 86
Cuts at: 2883 2895 3241 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 381 346 335 332 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 932 932
Size: 3689
DraI TTT'AAA
Cuts at: 688 688
Size: 3689
DrdI GACnn_nn'nnGTC
Cuts at: 1395 2253 1395
Size: 858 2831
EaeI y'GGCC_r
Cuts at: 258 1274 1448 1839 1866 3211 258
Size: 1016 174 391 27 1345 736
Fragments arranged by size:
1345 1016 736 391 174 27
EagI C'GGCC_G
Cuts at: 1274 1274
Size: 3689
EarI CTCTTCn'nnn_
Cuts at: 413 1712 1922 413
Size: 1299 210 2180
Fragments arranged by size:
2180 1299 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 607 1897 2207 2353 3499 607
Size: 1290 310 146 1146 797
Fragments arranged by size:
1290 1146 797 310 146
EcoICRI GAG'CTC
Cuts at: 236 701 236
Size: 465 3224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 367 505 602 1514 1946 2693 2961 278
Size: 89 138 97 912 432 747 268 1006
Fragments arranged by size:
1006 912 747 432 268 138 97 89
EcoO109I rG'GnC_Cy
Cuts at: 264 344 345 426 528 828 264
Size: 80 1 81 102 300 3125
Fragments arranged by size:
3125 300 102 81 80 1
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1708 1708
Size: 3689
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 1123 1371 2393 3372 1123
Size: 248 1022 979 1440
Fragments arranged by size:
1440 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 1531 1561 1563 1593 529
Size: 32 970 30 2 30 2625
Fragments arranged by size:
2625 970 32 30 30 2
HincII GTy'rAC
Cuts at: 728 728
Size: 3689
KasI G'GCGC_C
Cuts at: 1119 1367 1119
Size: 248 3441
KpnI G_GTAC'C
Cuts at: 709 709
Size: 3689
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2544 2360
Size: 184 3505
MscI TGG'CCA
Cuts at: 260 1450 260
Size: 1190 2499
MslI CAynn'nnrTG
Cuts at: 1678 1805 1678
Size: 127 3562
MspA1I CmG'CkG
Cuts at: 477 493 800 1114 1474 2487 2732 477
Size: 16 307 314 360 1013 245 1434
Fragments arranged by size:
1434 1013 360 314 307 245 16
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 1120 1368 1120
Size: 248 3441
NcoI C'CATG_G
Cuts at: 107 107
Size: 3689
NdeI CA'TA_TG
Cuts at: 364 364
Size: 3689
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NsiI A_TGCA'T
Cuts at: 670 670
Size: 3689
NspI r_CATG'y
Cuts at: 192 742 1242 2149 192
Size: 550 500 907 1732
Fragments arranged by size:
1732 907 550 500
PciI A'CATG_T
Cuts at: 2145 2145
Size: 3689
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 3657
PpuMI rG'GwC_Cy
Cuts at: 426 426
Size: 3689
PspOMI G'GGCC_C
Cuts at: 344 344
Size: 3689
PstI C_TGCA'G
Cuts at: 197 419 736 1421 197
Size: 222 317 685 2465
Fragments arranged by size:
2465 685 317 222
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3689
PvuII CAG'CTG
Cuts at: 1114 1474 1114
Size: 360 3329
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 238 703 238
Size: 465 3224
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 193 415 732 1417 2410 2601 33
Size: 160 222 317 685 993 191 1121
Fragments arranged by size:
1121 993 685 317 222 191 160
SfoI GGC'GCC
Cuts at: 1121 1369 1121
Size: 248 3441
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 711 711
Size: 3689
SmlI C'TyrA_G
Cuts at: 678 844 1759 2251 2513 678
Size: 166 915 492 262 1854
Fragments arranged by size:
1854 915 492 262 166
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StyI C'CwwG_G
Cuts at: 107 240 302 823 1177 107
Size: 133 62 521 354 2619
Fragments arranged by size:
2619 521 354 133 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1272 1405 1864 2073 1272
Size: 133 459 209 2888
Fragments arranged by size:
2888 459 209 133
TatI w'GTAC_w
Cuts at: 289 3204 289
Size: 2915 774
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 3657
Tth111I GACn'n_nGTC
Cuts at: 1486 1486
Size: 3689
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1184 1184
Size: 3689
XmaI C'CCGG_G
Cuts at: 709 709
Size: 3689
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PasI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI