(Circular) (Six-base) MAPSORT of: pF1KB7913.seq  Check: 1757  from: 1  to: 3596



pF1KB7913  3596 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:    634     634 
   Size:       3596
 
Acc65I G'GTAC_C

Cuts at:    612     612 
   Size:       3596
 
AclI AA'CG_TT

Cuts at:   3436    3436 
   Size:       3596
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    527    1421    1853    2600    2868     527 
   Size:        894     432     747     268    1255

  Fragments arranged by size: 

                1255     894     747     432     268
 
AflIII A'CryG_T

Cuts at:    482     948    2052    3029     482 
   Size:        466    1104     977    1049

  Fragments arranged by size: 

                1104    1049     977     466
 
AgeI A'CCGG_T

Cuts at:   1984    1984 
   Size:       3596
 
AlwNI CAG_nnn'CTG

Cuts at:    901    2468     901 
   Size:       1567    2029
 
ApaLI G'TGCA_C

Cuts at:   2366    2366 
   Size:       3596
 
ApoI r'AATT_y

Cuts at:    600    2949    3205     600 
   Size:       2349     256     991

  Fragments arranged by size: 

                2349     991     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3596
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3596
 
AvaI C'yCGr_G

Cuts at:    616     616 
   Size:       3596
 
BamHI G'GATC_C

Cuts at:    318     488     621     318 
   Size:        170     133    3293

  Fragments arranged by size: 

                3293     170     133
 
BanI G'GyrC_C

Cuts at:    612    1026    1274    1309     612 
   Size:        414     248      35    2899

  Fragments arranged by size: 

                2899     414     248      35
 
BanII G_rGCy'C

Cuts at:    610    1640     610 
   Size:       1030    2566
 
BbeI G_GCGC'C

Cuts at:   1030    1278    1030 
   Size:        248    3348
 
BbvCI CC'TCA_GC

Cuts at:    126     126 
   Size:       3596
 
BciVI GTATCCnnnnn_n'

Cuts at:   1494    2255    3596    1494 
   Size:        761    1341    1494

  Fragments arranged by size: 

                1494    1341     761
 
BclI T'GATC_A

Cuts at:   1116    1116 
   Size:       3596
 
BglII A'GATC_T

Cuts at:   1111    3148    1111 
   Size:       2037    1559
 
BlpI GC'TnA_GC

Cuts at:    708     708 
   Size:       3596
 
Bme1580I G_kGCm'C

Cuts at:   1221    1314    2370    1221 
   Size:         93    1056    2447

  Fragments arranged by size: 

                2447    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    808     889    1224    3368     808 
   Size:         81     335    2144    1036

  Fragments arranged by size: 

                2144    1036     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1963    1963 
   Size:       3596
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    460     460 
   Size:       3596
 
Bpu10I CC'TnA_GC

Cuts at:    126     126 
   Size:       3596
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    772    1651    2143    2441     772 
   Size:        879     492     298    1927

  Fragments arranged by size: 

                1927     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3300    3300 
   Size:       3596
 
BsaAI yAC'GTr

Cuts at:    949     949 
   Size:       3596
 
BsaBI GATnn'nnATC

Cuts at:      6    1110    3147       6 
   Size:       1104    2037     455

  Fragments arranged by size: 

                2037    1104     455
 
BsaHI Gr'CG_yC

Cuts at:   1027    1275    1977    3473    1027 
   Size:        248     702    1496    1150

  Fragments arranged by size: 

                1496    1150     702     248
 
BsaWI w'CCGG_w

Cuts at:    165     572     785    1306    1984    2258    2405    2816     165 
   Size:        407     213     521     678     274     147     411     945

  Fragments arranged by size: 

                 945     678     521     411     407     274     213     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    364     364 
   Size:       3596
 
BseYI C'CCAG_C

Cuts at:   1021    1779    2356    2852    1021 
   Size:        758     577     496    1765

  Fragments arranged by size: 

                1765     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1138    1184    2392     104 
   Size:       1034      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1034      46
 
BsiHKAI G_wGCw'C

Cuts at:    610    1388    1578    2370     610 
   Size:        778     190     792    1836

  Fragments arranged by size: 

                1836     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    610    1221    1314    1388    1578    1640    2370     610 
   Size:        611      93      74     190      62     730    1836

  Fragments arranged by size: 

                1836     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:    572    2816     572 
   Size:       2244    1352
 
BspHI T'CATG_A

Cuts at:   2772    2772 
   Size:       3596
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    646     860    1162    1543     646 
   Size:        214     302     381    2699

  Fragments arranged by size: 

                2699     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1888    1942    3426    3591      50 
   Size:       1838      54    1484     165      55

  Fragments arranged by size: 

                1838    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1508    1508 
   Size:       3596
 
BsrFI r'CCGG_y

Cuts at:   1594    1775    1984    1594 
   Size:        181     209    3206

  Fragments arranged by size: 

                3206     209     181
 
BssSI C'ACGA_G

Cuts at:   1867    2225    1867 
   Size:        358    3238
 
BstAPI GCAn_nnn'nTGC

Cuts at:    973     973 
   Size:       3596
 
BstBI TT'CG_AA

Cuts at:   1957    1957 
   Size:       3596
 
BstYI r'GATC_y

Cuts at:      1     318     488     621    1088    1111    1446    1692    2704 
   Size:        317     170     133     467      23     335     246    1012

Cuts at:   2704    2790    2802    3148       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     317     246     170
                 133      86      23      12
 
BtgI C'CryG_G

Cuts at:    197     197 
   Size:       3596
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    406    1546    1727    1890     406 
   Size:       1140     181     163    2112

  Fragments arranged by size: 

                2112    1140     181     163
 
BtsI GCAGTG_nn'

Cuts at:    221     839     221 
   Size:        618    2978
 
DraI TTT'AAA

Cuts at:    595     595 
   Size:       3596
 
DrdI GACnn_nn'nnGTC

Cuts at:   1302    2160    1302 
   Size:        858    2738
 
EaeI y'GGCC_r

Cuts at:    200    1181    1355    1746    1773    3118     200 
   Size:        981     174     391      27    1345     678

  Fragments arranged by size: 

                1345     981     678     391     174      27
 
EagI C'GGCC_G

Cuts at:   1181    1181 
   Size:       3596
 
EarI CTCTTCn'nnn_

Cuts at:   1619    1829    1619 
   Size:        210    3386
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1804    2114    2260    3406    1804 
   Size:        310     146    1146    1994

  Fragments arranged by size: 

                1994    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    608     608 
   Size:       3596
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    460     527    1421    1853    2600    2868     460 
   Size:         67     894     432     747     268    1188

  Fragments arranged by size: 

                1188     894     747     432     268      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:    188     242     188 
   Size:         54    3542
 
EcoO109I rG'GnC_Cy

Cuts at:    735     735 
   Size:       3596
 
EcoRI G'AATT_C

Cuts at:    600     600 
   Size:       3596
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1615    1615 
   Size:       3596
 
FspI TGC'GCA

Cuts at:   1377    1377 
   Size:       3596
 
HaeII r_GCGC'y

Cuts at:    474    1030    1278    2300    3279     474 
   Size:        556     248    1022     979     791

  Fragments arranged by size: 

                1022     979     791     556     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1438    1468    1470    1500    1438 
   Size:         30       2      30    3534

  Fragments arranged by size: 

                3534      30      30       2
 
HincII GTy'rAC

Cuts at:    635     635 
   Size:       3596
 
HindIII A'AGCT_T

Cuts at:    530     530 
   Size:       3596
 
KasI G'GCGC_C

Cuts at:   1026    1274    1026 
   Size:        248    3348
 
KpnI G_GTAC'C

Cuts at:    616     616 
   Size:       3596
 
MluI A'CGCG_T

Cuts at:   3029    3029 
   Size:       3596
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    314     540    2267    2451     314 
   Size:        226    1727     184    1459

  Fragments arranged by size: 

                1727    1459     226     184
 
MscI TGG'CCA

Cuts at:    202    1357     202 
   Size:       1155    2441
 
MslI CAynn'nnrTG

Cuts at:   1585    1712    1585 
   Size:        127    3469
 
MspA1I CmG'CkG

Cuts at:    459     707    1021    1381    2394    2639     459 
   Size:        248     314     360    1013     245    1416

  Fragments arranged by size: 

                1416    1013     360     314     248     245
 
NaeI GCC'GGC

Cuts at:   1777    1777 
   Size:       3596
 
NarI GG'CG_CC

Cuts at:   1027    1275    1027 
   Size:        248    3348
 
NcoI C'CATG_G

Cuts at:    197     197 
   Size:       3596
 
NgoMIV G'CCGG_C

Cuts at:   1775    1775 
   Size:       3596
 
NspI r_CATG'y

Cuts at:    486     649    1149    2056     486 
   Size:        163     500     907    2026

  Fragments arranged by size: 

                2026     907     500     163
 
PciI A'CATG_T

Cuts at:    482    2052     482 
   Size:       1570    2026
 
PflMI CCAn_nnn'nTGG

Cuts at:    499     499 
   Size:       3596
 
PmeI GTTT'AAAC

Cuts at:    595     595 
   Size:       3596
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2735    2767    2735 
   Size:         32    3564
 
PstI C_TGCA'G

Cuts at:    216     365     643    1328     216 
   Size:        149     278     685    2484

  Fragments arranged by size: 

                2484     685     278     149
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3596
 
PvuII CAG'CTG

Cuts at:    459    1021    1381     459 
   Size:        562     360    2674

  Fragments arranged by size: 

                2674     562     360
 
RsrII CG'GwC_CG

Cuts at:   1791    1791 
   Size:       3596
 
SacI G_AGCT'C

Cuts at:    610     610 
   Size:       3596
 
SalI G'TCGA_C

Cuts at:    633     633 
   Size:       3596
 
SapI GCTCTTCn'nnn_

Cuts at:   1619    1829    1619 
   Size:        210    3386
 
SbfI CC_TGCA'GG

Cuts at:    643     643 
   Size:       3596
 
ScaI AGT'ACT

Cuts at:    210    3113     210 
   Size:       2903     693
 
SfcI C'TryA_G

Cuts at:     33     212     361     639    1324    2317    2508      33 
   Size:        179     149     278     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     278     191     179     149
 
SfoI GGC'GCC

Cuts at:   1028    1276    1028 
   Size:        248    3348
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3596
 
SmaI CCC'GGG

Cuts at:    618     618 
   Size:       3596
 
SmlI C'TyrA_G

Cuts at:    751    1666    2158    2420     751 
   Size:        915     492     262    1927

  Fragments arranged by size: 

                1927     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3596
 
SphI G_CATG'C

Cuts at:    649    1149     649 
   Size:        500    3096
 
StyI C'CwwG_G

Cuts at:    197     730    1084     197 
   Size:        533     354    2709

  Fragments arranged by size: 

                2709     533     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1179    1312    1771    1980    1179 
   Size:        133     459     209    2795

  Fragments arranged by size: 

                2795     459     209     133
 
TatI w'GTAC_w

Cuts at:    208    3111     208 
   Size:       2903     693
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3113    3145    3113 
   Size:         32    3564
 
Tth111I GACn'n_nGTC

Cuts at:   1393    1393 
   Size:       3596
 
XbaI T'CTAG_A

Cuts at:    627     627 
   Size:       3596
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1091    1091 
   Size:       3596
 
XmaI C'CCGG_G

Cuts at:    616     616 
   Size:       3596
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI