Restriction map for
pF1KB7909
(Circular) (Six-base) MAPSORT of: pF1KB7909.seq Check: 7664 from: 1 to: 3515
pF1KB7909 3515 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 553 553
Size: 3515
Acc65I G'GTAC_C
Cuts at: 531 531
Size: 3515
AclI AA'CG_TT
Cuts at: 3355 3355
Size: 3515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 394 1340 1772 2519 2787 355
Size: 39 946 432 747 268 1083
Fragments arranged by size:
1083 946 747 432 268 39
AflIII A'CryG_T
Cuts at: 867 1971 2948 867
Size: 1104 977 1434
Fragments arranged by size:
1434 1104 977
AgeI A'CCGG_T
Cuts at: 1903 1903
Size: 3515
AleI CACnn'nnGTG
Cuts at: 472 472
Size: 3515
AlwNI CAG_nnn'CTG
Cuts at: 820 2387 820
Size: 1567 1948
ApaLI G'TGCA_C
Cuts at: 2285 2285
Size: 3515
ApoI r'AATT_y
Cuts at: 519 2868 3124 519
Size: 2349 256 910
Fragments arranged by size:
2349 910 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
AvaI C'yCGr_G
Cuts at: 535 535
Size: 3515
BamHI G'GATC_C
Cuts at: 223 433 540 223
Size: 210 107 3198
Fragments arranged by size:
3198 210 107
BanI G'GyrC_C
Cuts at: 531 945 1193 1228 531
Size: 414 248 35 2818
Fragments arranged by size:
2818 414 248 35
BanII G_rGCy'C
Cuts at: 395 529 1559 395
Size: 134 1030 2351
Fragments arranged by size:
2351 1030 134
BbeI G_GCGC'C
Cuts at: 949 1197 949
Size: 248 3267
BbsI GAAGACnn'nnnn_
Cuts at: 343 343
Size: 3515
BciVI GTATCCnnnnn_n'
Cuts at: 492 1413 2174 3515 492
Size: 921 761 1341 492
Fragments arranged by size:
1341 921 761 492
BclI T'GATC_A
Cuts at: 1035 1035
Size: 3515
BglI GCCn_nnn'nGGC
Cuts at: 128 450 128
Size: 322 3193
BglII A'GATC_T
Cuts at: 1030 3067 1030
Size: 2037 1478
BlpI GC'TnA_GC
Cuts at: 382 627 382
Size: 245 3270
Bme1580I G_kGCm'C
Cuts at: 446 467 1140 1233 2289 446
Size: 21 673 93 1056 1672
Fragments arranged by size:
1672 1056 673 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 727 808 1143 3287 727
Size: 81 335 2144 955
Fragments arranged by size:
2144 955 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1570 2062 2360 691
Size: 879 492 298 1846
Fragments arranged by size:
1846 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 3515
BsaAI yAC'GTr
Cuts at: 868 868
Size: 3515
BsaBI GATnn'nnATC
Cuts at: 6 1029 3066 6
Size: 1023 2037 455
Fragments arranged by size:
2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 946 1194 1896 3392 946
Size: 248 702 1496 1069
Fragments arranged by size:
1496 1069 702 248
BsaWI w'CCGG_w
Cuts at: 704 1225 1903 2177 2324 2735 704
Size: 521 678 274 147 411 1484
Fragments arranged by size:
1484 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 236 940 1698 2275 2771 236
Size: 704 758 577 496 980
Fragments arranged by size:
980 758 704 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 3515
BsiEI CG_ry'CG
Cuts at: 104 184 1057 1103 2311 104
Size: 80 873 46 1208 1308
Fragments arranged by size:
1308 1208 873 80 46
BsiHKAI G_wGCw'C
Cuts at: 529 1307 1497 2289 529
Size: 778 190 792 1755
Fragments arranged by size:
1755 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 395 446 467 529 1140 1233 1307 1497 1559
Size: 51 21 62 611 93 74 190 62
Cuts at: 1559 2289 395
Size: 730 1621
Fragments arranged by size:
1621 730 611 190 93 74 62 62
51 21
BspEI T'CCGG_A
Cuts at: 2735 2735
Size: 3515
BspHI T'CATG_A
Cuts at: 2691 2691
Size: 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 565 779 1081 1462 278
Size: 287 214 302 381 2331
Fragments arranged by size:
2331 381 302 287 214
BsrBI CCG'CTC
Cuts at: 50 1807 1861 3345 3510 50
Size: 1757 54 1484 165 55
Fragments arranged by size:
1757 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 1427
Size: 3515
BsrFI r'CCGG_y
Cuts at: 1513 1694 1903 1513
Size: 181 209 3125
Fragments arranged by size:
3125 209 181
BssHII G'CGCG_C
Cuts at: 131 131
Size: 3515
BssSI C'ACGA_G
Cuts at: 1786 2144 1786
Size: 358 3157
BstAPI GCAn_nnn'nTGC
Cuts at: 892 892
Size: 3515
BstBI TT'CG_AA
Cuts at: 1876 1876
Size: 3515
BstXI CCAn_nnnn'nTGG
Cuts at: 456 456
Size: 3515
BstYI r'GATC_y
Cuts at: 1 223 433 540 1007 1030 1365 1611 2623
Size: 222 210 107 467 23 335 246 1012
Cuts at: 2623 2709 2721 3067 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 222 210
107 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 218 218
Size: 3515
BtgI C'CryG_G
Cuts at: 107 467 107
Size: 360 3155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1465 1646 1809 1465
Size: 181 163 3171
Fragments arranged by size:
3171 181 163
BtsI GCAGTG_nn'
Cuts at: 467 758 467
Size: 291 3224
ClaI AT'CG_AT
Cuts at: 320 320
Size: 3515
DraI TTT'AAA
Cuts at: 514 514
Size: 3515
DrdI GACnn_nn'nnGTC
Cuts at: 1221 2079 1221
Size: 858 2657
EaeI y'GGCC_r
Cuts at: 181 1100 1274 1665 1692 3037 181
Size: 919 174 391 27 1345 659
Fragments arranged by size:
1345 919 659 391 174 27
EagI C'GGCC_G
Cuts at: 181 1100 181
Size: 919 2596
EarI CTCTTCn'nnn_
Cuts at: 401 1538 1748 401
Size: 1137 210 2168
Fragments arranged by size:
2168 1137 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 427 1723 2033 2179 3325 427
Size: 1296 310 146 1146 617
Fragments arranged by size:
1296 1146 617 310 146
EcoICRI GAG'CTC
Cuts at: 527 527
Size: 3515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 394 1340 1772 2519 2787 355
Size: 39 946 432 747 268 1083
Fragments arranged by size:
1083 946 747 432 268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 306 306
Size: 3515
EcoO109I rG'GnC_Cy
Cuts at: 486 654 486
Size: 168 3347
EcoRI G'AATT_C
Cuts at: 519 519
Size: 3515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 360 1534 360
Size: 1174 2341
FspI TGC'GCA
Cuts at: 1296 1296
Size: 3515
HaeII r_GCGC'y
Cuts at: 949 1197 2219 3198 949
Size: 248 1022 979 1266
Fragments arranged by size:
1266 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1357 1387 1389 1419 1357
Size: 30 2 30 3453
Fragments arranged by size:
3453 30 30 2
HincII GTy'rAC
Cuts at: 554 554
Size: 3515
KasI G'GCGC_C
Cuts at: 945 1193 945
Size: 248 3267
KpnI G_GTAC'C
Cuts at: 535 535
Size: 3515
MluI A'CGCG_T
Cuts at: 2948 2948
Size: 3515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2370 2186
Size: 184 3331
MscI TGG'CCA
Cuts at: 1276 1276
Size: 3515
MslI CAynn'nnrTG
Cuts at: 472 1504 1631 472
Size: 1032 127 2356
Fragments arranged by size:
2356 1032 127
MspA1I CmG'CkG
Cuts at: 293 381 424 626 940 1300 2313 2558 293
Size: 88 43 202 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 360 314 245 202 88 43
NaeI GCC'GGC
Cuts at: 1696 1696
Size: 3515
NarI GG'CG_CC
Cuts at: 946 1194 946
Size: 248 3267
NcoI C'CATG_G
Cuts at: 107 107
Size: 3515
NgoMIV G'CCGG_C
Cuts at: 1694 1694
Size: 3515
NspI r_CATG'y
Cuts at: 568 1068 1975 568
Size: 500 907 2108
Fragments arranged by size:
2108 907 500
PciI A'CATG_T
Cuts at: 1971 1971
Size: 3515
PmeI GTTT'AAAC
Cuts at: 514 514
Size: 3515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2654 2686 2654
Size: 32 3483
PpuMI rG'GwC_Cy
Cuts at: 486 486
Size: 3515
PstI C_TGCA'G
Cuts at: 562 1247 562
Size: 685 2830
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3515
PvuII CAG'CTG
Cuts at: 381 940 1300 381
Size: 559 360 2596
Fragments arranged by size:
2596 559 360
RsrII CG'GwC_CG
Cuts at: 1710 1710
Size: 3515
SacI G_AGCT'C
Cuts at: 529 529
Size: 3515
SalI G'TCGA_C
Cuts at: 552 552
Size: 3515
SapI GCTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
SbfI CC_TGCA'GG
Cuts at: 562 562
Size: 3515
ScaI AGT'ACT
Cuts at: 3032 3032
Size: 3515
SfcI C'TryA_G
Cuts at: 33 396 558 1243 2236 2427 33
Size: 363 162 685 993 191 1121
Fragments arranged by size:
1121 993 685 363 191 162
SfoI GGC'GCC
Cuts at: 947 1195 947
Size: 248 3267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
SmaI CCC'GGG
Cuts at: 537 537
Size: 3515
SmlI C'TyrA_G
Cuts at: 670 1585 2077 2339 670
Size: 915 492 262 1846
Fragments arranged by size:
1846 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3515
SphI G_CATG'C
Cuts at: 568 1068 568
Size: 500 3015
StyI C'CwwG_G
Cuts at: 107 649 1003 107
Size: 542 354 2619
Fragments arranged by size:
2619 542 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1098 1231 1690 1899 1098
Size: 133 459 209 2714
Fragments arranged by size:
2714 459 209 133
TatI w'GTAC_w
Cuts at: 3030 3030
Size: 3515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 425 457 3032 3064 334
Size: 32 59 32 2575 32 785
Fragments arranged by size:
2575 785 59 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1312 1312
Size: 3515
XbaI T'CTAG_A
Cuts at: 546 546
Size: 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1010 1010
Size: 3515
XmaI C'CCGG_G
Cuts at: 535 535
Size: 3515
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI