Restriction map for
pF1KB7890
(Circular) (Six-base) MAPSORT of: pF1KB7890.seq Check: 4592 from: 1 to: 5069
pF1KB7890 5069 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 2107 2107
Size: 5069
Acc65I G'GTAC_C
Cuts at: 2085 2085
Size: 5069
AclI AA'CG_TT
Cuts at: 1797 4909 1797
Size: 3112 1957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1225 1580 1739 2036 2894 3326 4073 4341
Size: 378 355 159 297 858 432 747 268
Cuts at: 4341 847
Size: 1575
Fragments arranged by size:
1575 858 747 432 378 355 297 268
159
AfeI AGC'GCT
Cuts at: 1670 1670
Size: 5069
AflIII A'CryG_T
Cuts at: 2421 3525 4502 2421
Size: 1104 977 2988
Fragments arranged by size:
2988 1104 977
AgeI A'CCGG_T
Cuts at: 3457 3457
Size: 5069
AhdI GACnn_n'nnGTC
Cuts at: 678 678
Size: 5069
AleI CACnn'nnGTG
Cuts at: 1300 1300
Size: 5069
AlwNI CAG_nnn'CTG
Cuts at: 825 2374 3941 825
Size: 1549 1567 1953
Fragments arranged by size:
1953 1567 1549
ApaI G_GGCC'C
Cuts at: 1325 1325
Size: 5069
ApaLI G'TGCA_C
Cuts at: 3839 3839
Size: 5069
ApoI r'AATT_y
Cuts at: 797 815 1123 1686 2073 4422 4678 797
Size: 18 308 563 387 2349 256 1188
Fragments arranged by size:
2349 1188 563 387 308 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
AvaI C'yCGr_G
Cuts at: 231 2089 231
Size: 1858 3211
BamHI G'GATC_C
Cuts at: 1179 2094 1179
Size: 915 4154
BanI G'GyrC_C
Cuts at: 2085 2499 2747 2782 2085
Size: 414 248 35 4372
Fragments arranged by size:
4372 414 248 35
BanII G_rGCy'C
Cuts at: 1325 2083 3113 1325
Size: 758 1030 3281
Fragments arranged by size:
3281 1030 758
BbeI G_GCGC'C
Cuts at: 2503 2751 2503
Size: 248 4821
BbsI GAAGACnn'nnnn_
Cuts at: 263 1242 1418 2015 263
Size: 979 176 597 3317
Fragments arranged by size:
3317 979 597 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 847 881 847
Size: 34 5035
BciVI GTATCCnnnnn_n'
Cuts at: 2967 3728 5069 2967
Size: 761 1341 2967
Fragments arranged by size:
2967 1341 761
BclI T'GATC_A
Cuts at: 617 2589 617
Size: 1972 3097
BfrBI ATG'CAT
Cuts at: 1860 1860
Size: 5069
BglI GCCn_nnn'nGGC
Cuts at: 1632 1632
Size: 5069
BglII A'GATC_T
Cuts at: 417 2584 4621 417
Size: 2167 2037 865
Fragments arranged by size:
2167 2037 865
BlpI GC'TnA_GC
Cuts at: 2181 2181
Size: 5069
Bme1580I G_kGCm'C
Cuts at: 239 1325 2694 2787 3843 239
Size: 1086 1369 93 1056 1465
Fragments arranged by size:
1465 1369 1086 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1228 2281 2362 2697 4841 1228
Size: 1053 81 335 2144 1456
Fragments arranged by size:
2144 1456 1053 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 5069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 406 965 1103 1268 1283 1629 1762 1898
Size: 178 559 138 165 15 346 133 136
Cuts at: 1898 228
Size: 3399
Fragments arranged by size:
3399 559 346 178 165 138 136 133
15
Bpu10I CC'TnA_GC
Cuts at: 1768 1768
Size: 5069
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1637 2245 3124 3616 3914 1637
Size: 608 879 492 298 2792
Fragments arranged by size:
2792 879 608 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 383 742 1747 4773 383
Size: 359 1005 3026 679
Fragments arranged by size:
3026 1005 679 359
BsaAI yAC'GTr
Cuts at: 1460 1934 2422 1460
Size: 474 488 4107
Fragments arranged by size:
4107 488 474
BsaBI GATnn'nnATC
Cuts at: 6 2583 4620 6
Size: 2577 2037 455
Fragments arranged by size:
2577 2037 455
BsaHI Gr'CG_yC
Cuts at: 2500 2748 3450 4946 2500
Size: 248 702 1496 2623
Fragments arranged by size:
2623 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2258 2779 3457 3731 3878 4289 2258
Size: 521 678 274 147 411 3038
Fragments arranged by size:
3038 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 5069
BseYI C'CCAG_C
Cuts at: 976 2494 3252 3829 4325 976
Size: 1518 758 577 496 1720
Fragments arranged by size:
1720 1518 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2611 2657 3865 104
Size: 2507 46 1208 1308
Fragments arranged by size:
2507 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1807 2083 2861 3051 3843 1807
Size: 276 778 190 792 3033
Fragments arranged by size:
3033 792 778 276 190
BsmI GAATG_Cn'
Cuts at: 1521 1521
Size: 5069
Bsp1286I G_dGCh'C
Cuts at: 239 1325 1807 2083 2694 2787 2861 3051 3113
Size: 1086 482 276 611 93 74 190 62
Cuts at: 3113 3843 239
Size: 730 1465
Fragments arranged by size:
1465 1086 730 611 482 276 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4289 4289
Size: 5069
BspHI T'CATG_A
Cuts at: 270 4245 270
Size: 3975 1094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2119 2333 2635 3016 2119
Size: 214 302 381 4172
Fragments arranged by size:
4172 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3361 3415 4899 5064 50
Size: 3311 54 1484 165 55
Fragments arranged by size:
3311 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1552 1958 2981 1552
Size: 406 1023 3640
Fragments arranged by size:
3640 1023 406
BsrFI r'CCGG_y
Cuts at: 3067 3248 3457 3067
Size: 181 209 4679
Fragments arranged by size:
4679 209 181
BssSI C'ACGA_G
Cuts at: 3340 3698 3340
Size: 358 4711
BstAPI GCAn_nnn'nTGC
Cuts at: 2446 2446
Size: 5069
BstBI TT'CG_AA
Cuts at: 254 1944 3430 254
Size: 1690 1486 1893
Fragments arranged by size:
1893 1690 1486
BstYI r'GATC_y
Cuts at: 1 417 558 1179 2094 2561 2584 2919 3165
Size: 416 141 621 915 467 23 335 246
Cuts at: 3165 4177 4263 4275 4621 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 915 621 467 449 416 346 335
246 141 86 23 12
BtgI C'CryG_G
Cuts at: 107 221 107
Size: 114 4955
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3019 3200 3363 3019
Size: 181 163 4725
Fragments arranged by size:
4725 181 163
BtsI GCAGTG_nn'
Cuts at: 2312 2312
Size: 5069
DraI TTT'AAA
Cuts at: 1026 2068 1026
Size: 1042 4027
DraIII CAC_nnn'GTG
Cuts at: 901 901
Size: 5069
DrdI GACnn_nn'nnGTC
Cuts at: 2775 3633 2775
Size: 858 4211
EaeI y'GGCC_r
Cuts at: 1454 2654 2828 3219 3246 4591 1454
Size: 1200 174 391 27 1345 1932
Fragments arranged by size:
1932 1345 1200 391 174 27
EagI C'GGCC_G
Cuts at: 2654 2654
Size: 5069
EarI CTCTTCn'nnn_
Cuts at: 256 351 3092 3302 256
Size: 95 2741 210 2023
Fragments arranged by size:
2741 2023 210 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3277 3587 3733 4879 3277
Size: 310 146 1146 3467
Fragments arranged by size:
3467 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2081 2081
Size: 5069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 406 847 965 1103 1225 1268 1283 1580
Size: 178 441 118 138 122 43 15 297
Cuts at: 1580 1629 1739 1762 1898 2036 2894 3326 4073
Size: 49 110 23 136 138 858 432 747
Cuts at: 4073 4341 228
Size: 268 956
Fragments arranged by size:
956 858 747 441 432 297 268 178
138 138 136 122 118 110 49 43
23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 151 151
Size: 5069
EcoO109I rG'GnC_Cy
Cuts at: 1322 1529 1840 2208 1322
Size: 207 311 368 4183
Fragments arranged by size:
4183 368 311 207
EcoRI G'AATT_C
Cuts at: 815 2073 815
Size: 1258 3811
EcoRV GAT'ATC
Cuts at: 1923 1923
Size: 5069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 720 1027 3088 720
Size: 307 2061 2701
Fragments arranged by size:
2701 2061 307
FspI TGC'GCA
Cuts at: 2850 2850
Size: 5069
HaeII r_GCGC'y
Cuts at: 1672 2503 2751 3773 4752 1672
Size: 831 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 831 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 575 664 696 1869 1901 2911 2941 2943
Size: 32 89 32 1173 32 1010 30 2
Cuts at: 2943 2973 543
Size: 30 2639
Fragments arranged by size:
2639 1173 1010 89 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2108 2108
Size: 5069
HindIII A'AGCT_T
Cuts at: 521 521
Size: 5069
KasI G'GCGC_C
Cuts at: 2499 2747 2499
Size: 248 4821
KpnI G_GTAC'C
Cuts at: 2089 2089
Size: 5069
MfeI C'AATT_G
Cuts at: 544 544
Size: 5069
MluI A'CGCG_T
Cuts at: 4502 4502
Size: 5069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 116 3740 3924 116
Size: 3624 184 1261
Fragments arranged by size:
3624 1261 184
MscI TGG'CCA
Cuts at: 1456 2830 1456
Size: 1374 3695
MslI CAynn'nnrTG
Cuts at: 1300 3058 3185 1300
Size: 1758 127 3184
Fragments arranged by size:
3184 1758 127
MspA1I CmG'CkG
Cuts at: 2180 2494 2854 3867 4112 2180
Size: 314 360 1013 245 3137
Fragments arranged by size:
3137 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3250 3250
Size: 5069
NarI GG'CG_CC
Cuts at: 2500 2748 2500
Size: 248 4821
NcoI C'CATG_G
Cuts at: 107 107
Size: 5069
NgoMIV G'CCGG_C
Cuts at: 3248 3248
Size: 5069
NsiI A_TGCA'T
Cuts at: 1862 1862
Size: 5069
NspI r_CATG'y
Cuts at: 2122 2622 3529 2122
Size: 500 907 3662
Fragments arranged by size:
3662 907 500
PciI A'CATG_T
Cuts at: 3525 3525
Size: 5069
PflMI CCAn_nnn'nTGG
Cuts at: 1176 1176
Size: 5069
PfoI T'CCnGG_A
Cuts at: 1525 1778 1525
Size: 253 4816
PmeI GTTT'AAAC
Cuts at: 2068 2068
Size: 5069
PmlI CAC'GTG
Cuts at: 1460 1460
Size: 5069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 5037
PpuMI rG'GwC_Cy
Cuts at: 1529 1840 1529
Size: 311 4758
PshAI GACnn'nnGTC
Cuts at: 1242 1242
Size: 5069
PsiI TTA'TAA
Cuts at: 761 761
Size: 5069
PspOMI G'GGCC_C
Cuts at: 1321 1321
Size: 5069
PstI C_TGCA'G
Cuts at: 2116 2801 2116
Size: 685 4384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5069
PvuII CAG'CTG
Cuts at: 2494 2854 2494
Size: 360 4709
RsrII CG'GwC_CG
Cuts at: 3264 3264
Size: 5069
SacI G_AGCT'C
Cuts at: 2083 2083
Size: 5069
SalI G'TCGA_C
Cuts at: 2106 2106
Size: 5069
SanDI GG'GwC_CC
Cuts at: 1840 1840
Size: 5069
SapI GCTCTTCn'nnn_
Cuts at: 3092 3302 3092
Size: 210 4859
SbfI CC_TGCA'GG
Cuts at: 2116 2116
Size: 5069
ScaI AGT'ACT
Cuts at: 1650 4586 1650
Size: 2936 2133
SexAI A'CCwGG_T
Cuts at: 2052 2052
Size: 5069
SfcI C'TryA_G
Cuts at: 33 1326 2112 2797 3790 3981 33
Size: 1293 786 685 993 191 1121
Fragments arranged by size:
1293 1121 993 786 685 191
SfoI GGC'GCC
Cuts at: 2501 2749 2501
Size: 248 4821
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
SmaI CCC'GGG
Cuts at: 2091 2091
Size: 5069
SmlI C'TyrA_G
Cuts at: 1652 2224 3139 3631 3893 1652
Size: 572 915 492 262 2828
Fragments arranged by size:
2828 915 572 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5069
SphI G_CATG'C
Cuts at: 2122 2622 2122
Size: 500 4569
SspI AAT'ATT
Cuts at: 1733 1733
Size: 5069
StyI C'CwwG_G
Cuts at: 107 2203 2557 107
Size: 2096 354 2619
Fragments arranged by size:
2619 2096 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 644 2652 2785 3244 3453 644
Size: 2008 133 459 209 2260
Fragments arranged by size:
2260 2008 459 209 133
TatI w'GTAC_w
Cuts at: 718 1648 4584 718
Size: 930 2936 1203
Fragments arranged by size:
2936 1203 930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4586 4618 4586
Size: 32 5037
Tth111I GACn'n_nGTC
Cuts at: 2866 2866
Size: 5069
XbaI T'CTAG_A
Cuts at: 2100 2100
Size: 5069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2564 2564
Size: 5069
XmaI C'CCGG_G
Cuts at: 2089 2089
Size: 5069
XmnI GAAnn'nnTTC
Cuts at: 815 815
Size: 5069
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI AscI AvrII BaeI
BbvCI BmgBI BmtI BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI