Restriction map for
pF1KB7879
(Circular) (Six-base) MAPSORT of: pF1KB7879.seq Check: 3153 from: 1 to: 4838
pF1KB7879 4838 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 349 349
Size: 4838
AccI GT'mk_AC
Cuts at: 1876 1876
Size: 4838
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 855 2663 3095 3842 4110 753
Size: 102 1808 432 747 268 1481
Fragments arranged by size:
1808 1481 747 432 268 102
AflIII A'CryG_T
Cuts at: 2190 3294 4271 2190
Size: 1104 977 2757
Fragments arranged by size:
2757 1104 977
AgeI A'CCGG_T
Cuts at: 3226 3226
Size: 4838
AleI CACnn'nnGTG
Cuts at: 386 386
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 118 462 2143 3710 118
Size: 344 1681 1567 1246
Fragments arranged by size:
1681 1567 1246 344
ApaLI G'TGCA_C
Cuts at: 3608 3608
Size: 4838
ApoI r'AATT_y
Cuts at: 317 544 1727 1842 4191 4447 317
Size: 227 1183 115 2349 256 708
Fragments arranged by size:
2349 1183 708 256 227 115
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 1858 1858
Size: 4838
AvrII C'CTAG_G
Cuts at: 352 352
Size: 4838
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 128 300 1854 2268 2516 2551 128
Size: 172 1554 414 248 35 2415
Fragments arranged by size:
2415 1554 414 248 172 35
BanII G_rGCy'C
Cuts at: 1852 2882 1852
Size: 1030 3808
BbeI G_GCGC'C
Cuts at: 132 2272 2520 132
Size: 2140 248 2450
Fragments arranged by size:
2450 2140 248
BbsI GAAGACnn'nnnn_
Cuts at: 190 612 694 190
Size: 422 82 4334
Fragments arranged by size:
4334 422 82
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3497 4838 2736
Size: 761 1341 2736
Fragments arranged by size:
2736 1341 761
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BfrBI ATG'CAT
Cuts at: 708 708
Size: 4838
BglII A'GATC_T
Cuts at: 2353 4390 2353
Size: 2037 2801
BlpI GC'TnA_GC
Cuts at: 1950 1950
Size: 4838
Bme1580I G_kGCm'C
Cuts at: 148 2463 2556 3612 148
Size: 2315 93 1056 1374
Fragments arranged by size:
2315 1374 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 644 1409 2050 2131 2466 4610 644
Size: 765 641 81 335 2144 872
Fragments arranged by size:
2144 872 765 641 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 365 3205 365
Size: 2840 1998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1169 1253 1337 1505 1589 1757 1085
Size: 84 84 84 168 84 168 4166
Fragments arranged by size:
4166 168 168 84 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 595 595
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2893 3385 3683 2014
Size: 879 492 298 3169
Fragments arranged by size:
3169 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4542 4542
Size: 4838
BsaAI yAC'GTr
Cuts at: 2191 2191
Size: 4838
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 129 2269 2517 3219 4715 129
Size: 2140 248 702 1496 252
Fragments arranged by size:
2140 1496 702 252 248
BsaWI w'CCGG_w
Cuts at: 2027 2548 3226 3500 3647 4058 2027
Size: 521 678 274 147 411 2807
Fragments arranged by size:
2807 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 178
Size: 30 4808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 310 1018 202
Size: 108 708 4022
Fragments arranged by size:
4022 708 108
BseYI C'CCAG_C
Cuts at: 267 2263 3021 3598 4094 267
Size: 1996 758 577 496 1011
Fragments arranged by size:
1996 1011 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 1692 852
Size: 840 3998
BsiEI CG_ry'CG
Cuts at: 104 2380 2426 3634 104
Size: 2276 46 1208 1308
Fragments arranged by size:
2276 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1852 2630 2820 3612 1852
Size: 778 190 792 3078
Fragments arranged by size:
3078 792 778 190
BsmI GAATG_Cn'
Cuts at: 1591 1759 1591
Size: 168 4670
Bsp1286I G_dGCh'C
Cuts at: 148 1852 2463 2556 2630 2820 2882 3612 148
Size: 1704 611 93 74 190 62 730 1374
Fragments arranged by size:
1704 1374 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4058 4058
Size: 4838
BspHI T'CATG_A
Cuts at: 1467 4014 1467
Size: 2547 2291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 349 1888 2102 2404 2785 349
Size: 1539 214 302 381 2402
Fragments arranged by size:
2402 1539 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3130 3184 4668 4833 50
Size: 3080 54 1484 165 55
Fragments arranged by size:
3080 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 2750
Size: 4838
BsrFI r'CCGG_y
Cuts at: 2836 3017 3226 2836
Size: 181 209 4448
Fragments arranged by size:
4448 209 181
BsrGI T'GTAC_A
Cuts at: 324 324
Size: 4838
BssSI C'ACGA_G
Cuts at: 3109 3467 3109
Size: 358 4480
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 2215
Size: 4838
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstEII G'GTnAC_C
Cuts at: 1117 1117
Size: 4838
BstYI r'GATC_y
Cuts at: 1 1366 1863 2330 2353 2688 2934 3946 4032
Size: 1365 497 467 23 335 246 1012 86
Cuts at: 4032 4044 4390 1
Size: 12 346 449
Fragments arranged by size:
1365 1012 497 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4838
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4494
Fragments arranged by size:
4494 181 163
BtsI GCAGTG_nn'
Cuts at: 500 840 893 1596 1680 2081 500
Size: 340 53 703 84 401 3257
Fragments arranged by size:
3257 703 401 340 84 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1326 1361 1326
Size: 35 4803
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DraIII CAC_nnn'GTG
Cuts at: 1542 1542
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3402 2544
Size: 858 3980
EaeI y'GGCC_r
Cuts at: 2423 2597 2988 3015 4360 2423
Size: 174 391 27 1345 2901
Fragments arranged by size:
2901 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2423 2423
Size: 4838
EarI CTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3046 3356 3502 4648 3046
Size: 310 146 1146 3236
Fragments arranged by size:
3236 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1850 1850
Size: 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 855 1085 1169 1253 1337 1505 1589 1757
Size: 102 230 84 84 84 168 84 168
Cuts at: 1757 2663 3095 3842 4110 753
Size: 906 432 747 268 1481
Fragments arranged by size:
1481 906 747 432 268 230 168 168
102 84 84 84 84
EcoNI CCTnn'n_nnAGG
Cuts at: 356 356
Size: 4838
EcoO109I rG'GnC_Cy
Cuts at: 904 1072 1325 1977 904
Size: 168 253 652 3765
Fragments arranged by size:
3765 652 253 168
EcoRI G'AATT_C
Cuts at: 1727 1842 1727
Size: 115 4723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1221 2857 1221
Size: 1636 3202
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 132 2272 2520 3542 4521 132
Size: 2140 248 1022 979 449
Fragments arranged by size:
2140 1022 979 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 978 1010 1146 1178 1358 1390 1536 1568 2680
Size: 32 136 32 180 32 146 32 1112
Cuts at: 2680 2710 2712 2742 978
Size: 30 2 30 3074
Fragments arranged by size:
3074 1112 180 146 136 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1877 1877
Size: 4838
KasI G'GCGC_C
Cuts at: 128 2268 2516 128
Size: 2140 248 2450
Fragments arranged by size:
2450 2140 248
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MfeI C'AATT_G
Cuts at: 1416 1416
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3509 3693 3509
Size: 184 4654
MscI TGG'CCA
Cuts at: 2599 2599
Size: 4838
MslI CAynn'nnrTG
Cuts at: 386 1220 1500 2827 2954 386
Size: 834 280 1327 127 2270
Fragments arranged by size:
2270 1327 834 280 127
MspA1I CmG'CkG
Cuts at: 115 1949 2263 2623 3636 3881 115
Size: 1834 314 360 1013 245 1072
Fragments arranged by size:
1834 1072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3019 3019
Size: 4838
NarI GG'CG_CC
Cuts at: 129 2269 2517 129
Size: 2140 248 2450
Fragments arranged by size:
2450 2140 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4838
NdeI CA'TA_TG
Cuts at: 1497 1497
Size: 4838
NgoMIV G'CCGG_C
Cuts at: 3017 3017
Size: 4838
NsiI A_TGCA'T
Cuts at: 710 710
Size: 4838
NspI r_CATG'y
Cuts at: 482 1891 2391 3298 482
Size: 1409 500 907 2022
Fragments arranged by size:
2022 1409 907 500
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4838
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 904 904
Size: 4838
PsiI TTA'TAA
Cuts at: 1667 1667
Size: 4838
PstI C_TGCA'G
Cuts at: 1885 2570 1885
Size: 685 4153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 2263 2623 2263
Size: 360 4478
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 1852 1852
Size: 4838
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SfcI C'TryA_G
Cuts at: 33 549 680 1881 2566 3559 3750 33
Size: 516 131 1201 685 993 191 1121
Fragments arranged by size:
1201 1121 993 685 516 191 131
SfoI GGC'GCC
Cuts at: 130 2270 2518 130
Size: 2140 248 2450
Fragments arranged by size:
2450 2140 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SmaI CCC'GGG
Cuts at: 1860 1860
Size: 4838
SmlI C'TyrA_G
Cuts at: 1993 2908 3400 3662 1993
Size: 915 492 262 3169
Fragments arranged by size:
3169 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
StuI AGG'CCT
Cuts at: 1242 1746 1828 1242
Size: 504 82 4252
Fragments arranged by size:
4252 504 82
StyI C'CwwG_G
Cuts at: 107 352 1972 2326 107
Size: 245 1620 354 2619
Fragments arranged by size:
2619 1620 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2421 2554 3013 3222 2421
Size: 133 459 209 4037
Fragments arranged by size:
4037 459 209 133
TatI w'GTAC_w
Cuts at: 324 4353 324
Size: 4029 809
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 812 844 4355 4387 178
Size: 32 602 32 3511 32 629
Fragments arranged by size:
3511 629 602 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4838
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2333
Size: 4838
XmaI C'CCGG_G
Cuts at: 1858 1858
Size: 4838
XmnI GAAnn'nnTTC
Cuts at: 805 805
Size: 4838
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HindIII HpaI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI