Restriction map for
pF1KB7878
(Circular) (Six-base) MAPSORT of: pF1KB7878.seq Check: 8219 from: 1 to: 5030
pF1KB7878 5030 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 907 907
Size: 5030
AccI GT'mk_AC
Cuts at: 1914 2068 1914
Size: 154 4876
Acc65I G'GTAC_C
Cuts at: 2046 2046
Size: 5030
AclI AA'CG_TT
Cuts at: 4870 4870
Size: 5030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1378 2855 3287 4034 4302 1190
Size: 188 1477 432 747 268 1918
Fragments arranged by size:
1918 1477 747 432 268 188
AflIII A'CryG_T
Cuts at: 761 2382 3486 4463 761
Size: 1621 1104 977 1328
Fragments arranged by size:
1621 1328 1104 977
AgeI A'CCGG_T
Cuts at: 3418 3418
Size: 5030
AhdI GACnn_n'nnGTC
Cuts at: 549 549
Size: 5030
AlwNI CAG_nnn'CTG
Cuts at: 1924 2335 3902 1924
Size: 411 1567 3052
Fragments arranged by size:
3052 1567 411
ApaLI G'TGCA_C
Cuts at: 3800 3800
Size: 5030
ApoI r'AATT_y
Cuts at: 1939 2034 4383 4639 1939
Size: 95 2349 256 2330
Fragments arranged by size:
2349 2330 256 95
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 2050 2050
Size: 5030
BamHI G'GATC_C
Cuts at: 135 219 2055 135
Size: 84 1836 3110
Fragments arranged by size:
3110 1836 84
BanI G'GyrC_C
Cuts at: 2046 2460 2708 2743 2046
Size: 414 248 35 4333
Fragments arranged by size:
4333 414 248 35
BanII G_rGCy'C
Cuts at: 2044 3074 2044
Size: 1030 4000
BbeI G_GCGC'C
Cuts at: 2464 2712 2464
Size: 248 4782
BbsI GAAGACnn'nnnn_
Cuts at: 401 760 1358 1571 1969 401
Size: 359 598 213 398 3462
Fragments arranged by size:
3462 598 398 359 213
BbvCI CC'TCA_GC
Cuts at: 113 686 113
Size: 573 4457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 197 231 90
Size: 34 73 34 4889
Fragments arranged by size:
4889 73 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 375 2000 2928 3689 5030 375
Size: 1625 928 761 1341 375
Fragments arranged by size:
1625 1341 928 761 375
BclI T'GATC_A
Cuts at: 2550 2550
Size: 5030
BglII A'GATC_T
Cuts at: 198 2545 4582 198
Size: 2347 2037 646
Fragments arranged by size:
2347 2037 646
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 2655 2748 3804 2655
Size: 93 1056 3881
Fragments arranged by size:
3881 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 378 832 1734 2242 2323 2658 4802 378
Size: 454 902 508 81 335 2144 606
Fragments arranged by size:
2144 902 606 508 454 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3397 3397
Size: 5030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1579 1631 2006 340
Size: 1239 52 375 3364
Fragments arranged by size:
3364 1239 375 52
Bpu10I CC'TnA_GC
Cuts at: 113 686 113
Size: 573 4457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 3085 3577 3875 2206
Size: 879 492 298 3361
Fragments arranged by size:
3361 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1452 4734 1452
Size: 3282 1748
BsaAI yAC'GTr
Cuts at: 2383 2383
Size: 5030
BsaBI GATnn'nnATC
Cuts at: 6 2544 4581 6
Size: 2538 2037 455
Fragments arranged by size:
2538 2037 455
BsaHI Gr'CG_yC
Cuts at: 2461 2709 3411 4907 2461
Size: 248 702 1496 2584
Fragments arranged by size:
2584 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2219 2740 3418 3692 3839 4250 2219
Size: 521 678 274 147 411 2999
Fragments arranged by size:
2999 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1979 2009 1979
Size: 30 5000
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1195 1204 1270 1273 1582 1195
Size: 9 66 3 309 4643
Fragments arranged by size:
4643 309 66 9 3
BseYI C'CCAG_C
Cuts at: 1924 2455 3213 3790 4286 1924
Size: 531 758 577 496 2668
Fragments arranged by size:
2668 758 577 531 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 932
Size: 5030
BsiEI CG_ry'CG
Cuts at: 104 2572 2618 3826 104
Size: 2468 46 1208 1308
Fragments arranged by size:
2468 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 196 1112 2044 2822 3012 3804 196
Size: 916 932 778 190 792 1422
Fragments arranged by size:
1422 932 916 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 196 1112 2044 2655 2748 2822 3012 3074 3804
Size: 916 932 611 93 74 190 62 730
Cuts at: 3804 196
Size: 1422
Fragments arranged by size:
1422 932 916 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4250 4250
Size: 5030
BspHI T'CATG_A
Cuts at: 4206 4206
Size: 5030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 907 2080 2294 2596 2977 907
Size: 1173 214 302 381 2960
Fragments arranged by size:
2960 1173 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3322 3376 4860 5025 50
Size: 3272 54 1484 165 55
Fragments arranged by size:
3272 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1611 2942 1611
Size: 1331 3699
BsrFI r'CCGG_y
Cuts at: 851 3028 3209 3418 851
Size: 2177 181 209 2463
Fragments arranged by size:
2463 2177 209 181
BssSI C'ACGA_G
Cuts at: 3301 3659 3301
Size: 358 4672
BstAPI GCAn_nnn'nTGC
Cuts at: 2407 2407
Size: 5030
BstBI TT'CG_AA
Cuts at: 159 3391 159
Size: 3232 1798
BstXI CCAn_nnnn'nTGG
Cuts at: 1469 1469
Size: 5030
BstYI r'GATC_y
Cuts at: 1 135 198 219 2055 2522 2545 2880 3126
Size: 134 63 21 1836 467 23 335 246
Cuts at: 3126 4138 4224 4236 4582 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1836 1012 467 449 346 335 246 134
86 63 23 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2980 3161 3324 2980
Size: 181 163 4686
Fragments arranged by size:
4686 181 163
BtsI GCAGTG_nn'
Cuts at: 920 1236 2273 920
Size: 316 1037 3677
Fragments arranged by size:
3677 1037 316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 431 466 431
Size: 35 4995
DraI TTT'AAA
Cuts at: 984 2029 984
Size: 1045 3985
DrdI GACnn_nn'nnGTC
Cuts at: 2736 3594 2736
Size: 858 4172
EaeI y'GGCC_r
Cuts at: 2615 2789 3180 3207 4552 2615
Size: 174 391 27 1345 3093
Fragments arranged by size:
3093 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5030
EarI CTCTTCn'nnn_
Cuts at: 491 621 956 1100 1262 1268 1381 3053 3263
Size: 130 335 144 162 6 113 1672 210
Cuts at: 3263 491
Size: 2258
Fragments arranged by size:
2258 1672 335 210 162 144 130 113
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 128 3238 3548 3694 4840 128
Size: 3110 310 146 1146 318
Fragments arranged by size:
3110 1146 318 310 146
EcoICRI GAG'CTC
Cuts at: 2042 2042
Size: 5030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1190 1378 1579 1631 2006 2855 3287 4034
Size: 850 188 201 52 375 849 432 747
Cuts at: 4034 4302 340
Size: 268 1068
Fragments arranged by size:
1068 850 849 747 432 375 268 201
188 52
EcoNI CCTnn'n_nnAGG
Cuts at: 953 1018 1360 953
Size: 65 342 4623
Fragments arranged by size:
4623 342 65
EcoO109I rG'GnC_Cy
Cuts at: 690 1027 1770 1864 2169 690
Size: 337 743 94 305 3551
Fragments arranged by size:
3551 743 337 305 94
EcoRI G'AATT_C
Cuts at: 2034 2034
Size: 5030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 801 3049 801
Size: 2248 2782
FspI TGC'GCA
Cuts at: 2811 2811
Size: 5030
HaeII r_GCGC'y
Cuts at: 2464 2712 3734 4713 2464
Size: 248 1022 979 2781
Fragments arranged by size:
2781 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 183 215 535 567 933 965 1044 1076 1303
Size: 32 320 32 366 32 79 32 227
Cuts at: 1303 1335 1563 1595 2872 2902 2904 2934 183
Size: 32 228 32 1277 30 2 30 2279
Fragments arranged by size:
2279 1277 366 320 228 227 79 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1947 2069 1947
Size: 122 4908
HindIII A'AGCT_T
Cuts at: 1882 1882
Size: 5030
HpaI GTT'AAC
Cuts at: 1947 1947
Size: 5030
KasI G'GCGC_C
Cuts at: 2460 2708 2460
Size: 248 4782
KpnI G_GTAC'C
Cuts at: 2050 2050
Size: 5030
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 541 1157 3701 3885 541
Size: 616 2544 184 1686
Fragments arranged by size:
2544 1686 616 184
MscI TGG'CCA
Cuts at: 2791 2791
Size: 5030
MslI CAynn'nnrTG
Cuts at: 878 1467 3019 3146 878
Size: 589 1552 127 2762
Fragments arranged by size:
2762 1552 589 127
MspA1I CmG'CkG
Cuts at: 425 652 2141 2455 2815 3828 4073 425
Size: 227 1489 314 360 1013 245 1382
Fragments arranged by size:
1489 1382 1013 360 314 245 227
NaeI GCC'GGC
Cuts at: 853 3211 853
Size: 2358 2672
NarI GG'CG_CC
Cuts at: 2461 2709 2461
Size: 248 4782
NgoMIV G'CCGG_C
Cuts at: 851 3209 851
Size: 2358 2672
NspI r_CATG'y
Cuts at: 765 2083 2583 3490 765
Size: 1318 500 907 2305
Fragments arranged by size:
2305 1318 907 500
PciI A'CATG_T
Cuts at: 761 3486 761
Size: 2725 2305
PflMI CCAn_nnn'nTGG
Cuts at: 1924 1924
Size: 5030
PfoI T'CCnGG_A
Cuts at: 338 338
Size: 5030
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 690 1027 1864 690
Size: 337 837 3856
Fragments arranged by size:
3856 837 337
PshAI GACnn'nnGTC
Cuts at: 1648 1680 1648
Size: 32 4998
PsiI TTA'TAA
Cuts at: 505 505
Size: 5030
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 886 918 886
Size: 32 4998
PstI C_TGCA'G
Cuts at: 683 1971 2077 2762 683
Size: 1288 106 685 2951
Fragments arranged by size:
2951 1288 685 106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5030
PvuII CAG'CTG
Cuts at: 425 652 2455 2815 425
Size: 227 1803 360 2640
Fragments arranged by size:
2640 1803 360 227
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 2044 2044
Size: 5030
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 1100 3053 3263 1100
Size: 1953 210 2867
Fragments arranged by size:
2867 1953 210
SbfI CC_TGCA'GG
Cuts at: 2077 2077
Size: 5030
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SfcI C'TryA_G
Cuts at: 33 679 1346 1967 2073 2758 3751 3942 33
Size: 646 667 621 106 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 646 621 191 106
SfoI GGC'GCC
Cuts at: 2462 2710 2462
Size: 248 4782
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 2052 2052
Size: 5030
SmlI C'TyrA_G
Cuts at: 2185 3100 3592 3854 2185
Size: 915 492 262 3361
Fragments arranged by size:
3361 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 2083 2583 2083
Size: 500 4530
StuI AGG'CCT
Cuts at: 1991 1991
Size: 5030
StyI C'CwwG_G
Cuts at: 1678 2164 2518 1678
Size: 486 354 4190
Fragments arranged by size:
4190 486 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1132 1900 2613 2746 3205 3414 1132
Size: 768 713 133 459 209 2748
Fragments arranged by size:
2748 768 713 459 209 133
TatI w'GTAC_w
Cuts at: 1072 4545 1072
Size: 3473 1557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 4998
Tth111I GACn'n_nGTC
Cuts at: 706 2827 706
Size: 2121 2909
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1027 2525 1027
Size: 1498 3532
XmaI C'CCGG_G
Cuts at: 2050 2050
Size: 5030
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
DraIII EcoRV FseI FspAI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI