Restriction map for
pF1KB7876
(Circular) (Six-base) MAPSORT of: pF1KB7876.seq Check: 4927 from: 1 to: 5027
pF1KB7876 5027 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 2065 2065
Size: 5027
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 4867 4867
Size: 5027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 304 858 1278 1614 2852 3284 4031 4299
Size: 199 554 420 336 1238 432 747 268
Cuts at: 4299 105
Size: 833
Fragments arranged by size:
1238 833 747 554 432 420 336 268
199
AflIII A'CryG_T
Cuts at: 2379 3483 4460 2379
Size: 1104 977 2946
Fragments arranged by size:
2946 1104 977
AgeI A'CCGG_T
Cuts at: 3415 3415
Size: 5027
AlwNI CAG_nnn'CTG
Cuts at: 2332 3899 2332
Size: 1567 3460
ApaLI G'TGCA_C
Cuts at: 3797 3797
Size: 5027
ApoI r'AATT_y
Cuts at: 254 604 1652 2031 4380 4636 254
Size: 350 1048 379 2349 256 645
Fragments arranged by size:
2349 1048 645 379 350 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 2047 2047
Size: 5027
BamHI G'GATC_C
Cuts at: 2052 2052
Size: 5027
BanI G'GyrC_C
Cuts at: 2043 2457 2705 2740 2043
Size: 414 248 35 4330
Fragments arranged by size:
4330 414 248 35
BanII G_rGCy'C
Cuts at: 492 1561 2004 2041 3071 492
Size: 1069 443 37 1030 2448
Fragments arranged by size:
2448 1069 1030 443 37
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4993
BciVI GTATCCnnnnn_n'
Cuts at: 2925 3686 5027 2925
Size: 761 1341 2925
Fragments arranged by size:
2925 1341 761
BclI T'GATC_A
Cuts at: 2547 2547
Size: 5027
BfrBI ATG'CAT
Cuts at: 1354 1740 1824 1354
Size: 386 84 4557
Fragments arranged by size:
4557 386 84
BglII A'GATC_T
Cuts at: 1297 1633 2542 4579 1297
Size: 336 909 2037 1745
Fragments arranged by size:
2037 1745 909 336
BlpI GC'TnA_GC
Cuts at: 2139 2139
Size: 5027
Bme1580I G_kGCm'C
Cuts at: 2652 2745 3801 2652
Size: 93 1056 3878
Fragments arranged by size:
3878 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 758 842 962 1130 1466 2014 2239 2320 2655
Size: 84 120 168 336 548 225 81 335
Cuts at: 2655 4799 758
Size: 2144 986
Fragments arranged by size:
2144 986 548 336 335 225 168 120
84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 235 3394 235
Size: 3159 1868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 686 1106 1190 1274 1526 1610 1694 1778
Size: 366 420 84 84 252 84 84 84
Cuts at: 1778 1862 1946 320
Size: 84 84 3401
Fragments arranged by size:
3401 420 366 252 84 84 84 84
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2203 3082 3574 3872 2203
Size: 879 492 298 3358
Fragments arranged by size:
3358 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4731 4731
Size: 5027
BsaAI yAC'GTr
Cuts at: 2380 2380
Size: 5027
BsaBI GATnn'nnATC
Cuts at: 6 2541 4578 6
Size: 2535 2037 455
Fragments arranged by size:
2535 2037 455
BsaHI Gr'CG_yC
Cuts at: 2458 2706 3408 4904 2458
Size: 248 702 1496 2581
Fragments arranged by size:
2581 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2216 2737 3415 3689 3836 4247 2216
Size: 521 678 274 147 411 2996
Fragments arranged by size:
2996 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 4997
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 456 456
Size: 5027
BseYI C'CCAG_C
Cuts at: 2452 3210 3787 4283 2452
Size: 758 577 496 3196
Fragments arranged by size:
3196 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2569 2615 3823 104
Size: 2465 46 1208 1308
Fragments arranged by size:
2465 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 348 1561 2041 2819 3009 3801 348
Size: 1213 480 778 190 792 1574
Fragments arranged by size:
1574 1213 792 778 480 190
BsmI GAATG_Cn'
Cuts at: 529 787 955 1082 1528 1612 529
Size: 258 168 127 446 84 3944
Fragments arranged by size:
3944 446 258 168 127 84
Bsp1286I G_dGCh'C
Cuts at: 348 492 1561 2004 2041 2652 2745 2819 3009
Size: 144 1069 443 37 611 93 74 190
Cuts at: 3009 3071 3801 348
Size: 62 730 1574
Fragments arranged by size:
1574 1069 730 611 443 190 144 93
74 62 37
BspEI T'CCGG_A
Cuts at: 4247 4247
Size: 5027
BspHI T'CATG_A
Cuts at: 1236 4203 1236
Size: 2967 2060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2077 2291 2593 2974 2077
Size: 214 302 381 4130
Fragments arranged by size:
4130 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3319 3373 4857 5022 50
Size: 3269 54 1484 165 55
Fragments arranged by size:
3269 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1285 1621 1873 2939 1285
Size: 336 252 1066 3373
Fragments arranged by size:
3373 1066 336 252
BsrFI r'CCGG_y
Cuts at: 3025 3206 3415 3025
Size: 181 209 4637
Fragments arranged by size:
4637 209 181
BssSI C'ACGA_G
Cuts at: 3298 3656 3298
Size: 358 4669
BstAPI GCAn_nnn'nTGC
Cuts at: 2404 2404
Size: 5027
BstBI TT'CG_AA
Cuts at: 726 1902 3388 726
Size: 1176 1486 2365
Fragments arranged by size:
2365 1486 1176
BstYI r'GATC_y
Cuts at: 1 1297 1540 1633 1717 2052 2519 2542 2877
Size: 1296 243 93 84 335 467 23 335
Cuts at: 2877 3123 4135 4221 4233 4579 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1296 1012 467 449 346 335 335 246
243 93 86 84 23 12
BtgI C'CryG_G
Cuts at: 579 579
Size: 5027
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2977 3158 3321 2977
Size: 181 163 4683
Fragments arranged by size:
4683 181 163
BtsI GCAGTG_nn'
Cuts at: 327 348 1537 1705 1789 2004 2270 327
Size: 21 1189 168 84 215 266 3084
Fragments arranged by size:
3084 1189 266 215 168 84 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1179 1214 1515 1550 1599 1634 1179
Size: 35 301 35 49 35 4572
Fragments arranged by size:
4572 301 49 35 35 35
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DrdI GACnn_nn'nnGTC
Cuts at: 2733 3591 2733
Size: 858 4169
EaeI y'GGCC_r
Cuts at: 2612 2786 3177 3204 4549 2612
Size: 174 391 27 1345 3090
Fragments arranged by size:
3090 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2612 2612
Size: 5027
EarI CTCTTCn'nnn_
Cuts at: 885 1418 3050 3260 885
Size: 533 1632 210 2652
Fragments arranged by size:
2652 1632 533 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3545 3691 4837 3235
Size: 310 146 1146 3425
Fragments arranged by size:
3425 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1559 2039 1559
Size: 480 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 304 320 686 858 1106 1190 1274 1278
Size: 199 16 366 172 248 84 84 4
Cuts at: 1278 1526 1610 1614 1694 1778 1862 1946 2852
Size: 248 84 4 80 84 84 84 906
Cuts at: 2852 3284 4031 4299 105
Size: 432 747 268 833
Fragments arranged by size:
906 833 747 432 366 268 248 248
199 172 84 84 84 84 84 84
80 16 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 5027
EcoRI G'AATT_C
Cuts at: 2031 2031
Size: 5027
EcoRV GAT'ATC
Cuts at: 1094 1234 1570 1094
Size: 140 336 4551
Fragments arranged by size:
4551 336 140
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 5027
FspI TGC'GCA
Cuts at: 2808 2808
Size: 5027
HaeII r_GCGC'y
Cuts at: 2461 2709 3731 4710 2461
Size: 248 1022 979 2778
Fragments arranged by size:
2778 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2869 2899 2901 2931 2869
Size: 30 2 30 4965
Fragments arranged by size:
4965 30 30 2
HincII GTy'rAC
Cuts at: 2066 2066
Size: 5027
HindIII A'AGCT_T
Cuts at: 1877 1877
Size: 5027
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3698 3882 3698
Size: 184 4843
MscI TGG'CCA
Cuts at: 2788 2788
Size: 5027
MslI CAynn'nnrTG
Cuts at: 245 429 681 849 933 960 1487 3016 3143
Size: 184 252 168 84 27 527 1529 127
Cuts at: 3143 245
Size: 2129
Fragments arranged by size:
2129 1529 527 252 184 168 127 84
27
MspA1I CmG'CkG
Cuts at: 2138 2452 2812 3825 4070 2138
Size: 314 360 1013 245 3095
Fragments arranged by size:
3095 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3208 3208
Size: 5027
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NdeI CA'TA_TG
Cuts at: 396 846 930 396
Size: 450 84 4493
Fragments arranged by size:
4493 450 84
NgoMIV G'CCGG_C
Cuts at: 3206 3206
Size: 5027
NruI TCG'CGA
Cuts at: 1812 1812
Size: 5027
NsiI A_TGCA'T
Cuts at: 1356 1742 1826 1356
Size: 386 84 4557
Fragments arranged by size:
4557 386 84
NspI r_CATG'y
Cuts at: 282 806 1358 2080 2580 3487 282
Size: 524 552 722 500 907 1822
Fragments arranged by size:
1822 907 722 552 524 500
PciI A'CATG_T
Cuts at: 3483 3483
Size: 5027
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 118 150 4166 4198 118
Size: 32 4016 32 947
Fragments arranged by size:
4016 947 32 32
PsiI TTA'TAA
Cuts at: 596 764 596
Size: 168 4859
PstI C_TGCA'G
Cuts at: 2074 2759 2074
Size: 685 4342
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 2452 2812 2452
Size: 360 4667
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 1561 2041 1561
Size: 480 4547
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 885 3050 3260 885
Size: 2165 210 2652
Fragments arranged by size:
2652 2165 210
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 4544 4544
Size: 5027
SfcI C'TryA_G
Cuts at: 33 137 2070 2755 3748 3939 33
Size: 104 1933 685 993 191 1121
Fragments arranged by size:
1933 1121 993 685 191 104
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 2049 2049
Size: 5027
SmlI C'TyrA_G
Cuts at: 2182 3097 3589 3851 2182
Size: 915 492 262 3358
Fragments arranged by size:
3358 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 2080 2580 2080
Size: 500 4527
StyI C'CwwG_G
Cuts at: 2161 2515 2161
Size: 354 4673
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2610 2743 3202 3411 2610
Size: 133 459 209 4226
Fragments arranged by size:
4226 459 209 133
TatI w'GTAC_w
Cuts at: 4542 4542
Size: 5027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4544 4576 4544
Size: 32 4995
Tth111I GACn'n_nGTC
Cuts at: 2824 2824
Size: 5027
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2522 2522
Size: 5027
XmaI C'CCGG_G
Cuts at: 2047 2047
Size: 5027
XmnI GAAnn'nnTTC
Cuts at: 787 871 1291 1459 787
Size: 84 420 168 4355
Fragments arranged by size:
4355 420 168 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NruI NsiI NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI
HpaI MfeI NcoI NheI NotI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI