Restriction map for
pF1KB7875
(Circular) (Six-base) MAPSORT of: pF1KB7875.seq Check: 2181 from: 1 to: 4823
pF1KB7875 4823 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1861 1861
Size: 4823
Acc65I G'GTAC_C
Cuts at: 1839 1839
Size: 4823
AclI AA'CG_TT
Cuts at: 4663 4663
Size: 4823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 2648 3080 3827 4095 139
Size: 2509 432 747 268 867
Fragments arranged by size:
2509 867 747 432 268
AflIII A'CryG_T
Cuts at: 2175 3279 4256 2175
Size: 1104 977 2742
Fragments arranged by size:
2742 1104 977
AgeI A'CCGG_T
Cuts at: 1004 1088 1508 3211 1004
Size: 84 420 1703 2616
Fragments arranged by size:
2616 1703 420 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 171 171
Size: 4823
AlwNI CAG_nnn'CTG
Cuts at: 2128 3695 2128
Size: 1567 3256
ApaLI G'TGCA_C
Cuts at: 3593 3593
Size: 4823
ApoI r'AATT_y
Cuts at: 235 670 782 798 866 1034 1303 1370 1827
Size: 435 112 16 68 168 269 67 457
Cuts at: 1827 4176 4432 235
Size: 2349 256 626
Fragments arranged by size:
2349 626 457 435 269 256 168 112
68 67 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 1843 1843
Size: 4823
BamHI G'GATC_C
Cuts at: 1848 1848
Size: 4823
BanI G'GyrC_C
Cuts at: 1839 2253 2501 2536 1839
Size: 414 248 35 4126
Fragments arranged by size:
4126 414 248 35
BanII G_rGCy'C
Cuts at: 389 1837 2867 389
Size: 1448 1030 2345
Fragments arranged by size:
2345 1448 1030
BbeI G_GCGC'C
Cuts at: 2257 2505 2257
Size: 248 4575
BbsI GAAGACnn'nnnn_
Cuts at: 315 1673 315
Size: 1358 3465
BciVI GTATCCnnnnn_n'
Cuts at: 143 2721 3482 4823 143
Size: 2578 761 1341 143
Fragments arranged by size:
2578 1341 761 143
BclI T'GATC_A
Cuts at: 359 2343 359
Size: 1984 2839
BglII A'GATC_T
Cuts at: 2338 4375 2338
Size: 2037 2786
BlpI GC'TnA_GC
Cuts at: 272 1935 272
Size: 1663 3160
Bme1580I G_kGCm'C
Cuts at: 2448 2541 3597 2448
Size: 93 1056 3674
Fragments arranged by size:
3674 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 421 2035 2116 2451 4595 421
Size: 1614 81 335 2144 649
Fragments arranged by size:
2144 1614 649 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 4823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 265 896 980 1064 1148 1232 1316 1400
Size: 119 631 84 84 84 84 84 84
Cuts at: 1400 1484 1568 1736 1818 146
Size: 84 84 168 82 3151
Fragments arranged by size:
3151 631 168 119 84 84 84 84
84 84 84 84 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1999 2878 3370 3668 124
Size: 1875 879 492 298 1279
Fragments arranged by size:
1875 1279 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4527 4527
Size: 4823
BsaAI yAC'GTr
Cuts at: 2176 2176
Size: 4823
BsaBI GATnn'nnATC
Cuts at: 6 2337 4374 6
Size: 2331 2037 455
Fragments arranged by size:
2331 2037 455
BsaHI Gr'CG_yC
Cuts at: 2254 2502 3204 4700 2254
Size: 248 702 1496 2377
Fragments arranged by size:
2377 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1004 1088 1508 2012 2533 3211 3485 3632 4043
Size: 84 420 504 521 678 274 147 411
Cuts at: 4043 1004
Size: 1784
Fragments arranged by size:
1784 678 521 504 420 411 274 147
84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 262
Size: 4823
BseYI C'CCAG_C
Cuts at: 2248 3006 3583 4079 2248
Size: 758 577 496 2992
Fragments arranged by size:
2992 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1427 1511 562
Size: 865 84 3874
Fragments arranged by size:
3874 865 84
BsiEI CG_ry'CG
Cuts at: 104 2365 2411 3619 104
Size: 2261 46 1208 1308
Fragments arranged by size:
2261 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1837 2615 2805 3597 1837
Size: 778 190 792 3063
Fragments arranged by size:
3063 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 389 1837 2448 2541 2615 2805 2867 3597 389
Size: 1448 611 93 74 190 62 730 1615
Fragments arranged by size:
1615 1448 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4043 4043
Size: 4823
BspHI T'CATG_A
Cuts at: 3999 3999
Size: 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 322 1873 2087 2389 2770 322
Size: 1551 214 302 381 2375
Fragments arranged by size:
2375 1551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4653 4818 50
Size: 3065 54 1484 165 55
Fragments arranged by size:
3065 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 262 2735 262
Size: 2473 2350
BsrFI r'CCGG_y
Cuts at: 1004 1088 1508 2821 3002 3211 1004
Size: 84 420 1313 181 209 2616
Fragments arranged by size:
2616 1313 420 209 181 84
BssSI C'ACGA_G
Cuts at: 3094 3452 3094
Size: 358 4465
BstAPI GCAn_nnn'nTGC
Cuts at: 337 2200 337
Size: 1863 2960
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstYI r'GATC_y
Cuts at: 1 1848 2315 2338 2673 2919 3931 4017 4029
Size: 1847 467 23 335 246 1012 86 12
Cuts at: 4029 4375 1
Size: 346 449
Fragments arranged by size:
1847 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 424 2773 2954 3117 424
Size: 2349 181 163 2130
Fragments arranged by size:
2349 2130 181 163
BtsI GCAGTG_nn'
Cuts at: 266 2066 266
Size: 1800 3023
DraI TTT'AAA
Cuts at: 1822 1822
Size: 4823
DrdI GACnn_nn'nnGTC
Cuts at: 2529 3387 2529
Size: 858 3965
EaeI y'GGCC_r
Cuts at: 227 460 2408 2582 2973 3000 4345 227
Size: 233 1948 174 391 27 1345 705
Fragments arranged by size:
1948 1345 705 391 233 174 27
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 394 2846 3056 394
Size: 2452 210 2161
Fragments arranged by size:
2452 2161 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3031 3341 3487 4633 3031
Size: 310 146 1146 3221
Fragments arranged by size:
3221 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1835 1835
Size: 4823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 146 265 896 980 1064 1148 1232 1316
Size: 7 119 631 84 84 84 84 84
Cuts at: 1316 1400 1484 1568 1736 1818 2648 3080 3827
Size: 84 84 84 168 82 830 432 747
Cuts at: 3827 4095 139
Size: 268 867
Fragments arranged by size:
867 830 747 631 432 268 168 119
84 84 84 84 84 84 84 84
82 7
EcoO109I rG'GnC_Cy
Cuts at: 1962 1962
Size: 4823
EcoRI G'AATT_C
Cuts at: 782 866 1034 1370 1827 782
Size: 84 168 336 457 3778
Fragments arranged by size:
3778 457 336 168 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4823
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4823
HaeII r_GCGC'y
Cuts at: 2257 2505 3527 4506 2257
Size: 248 1022 979 2574
Fragments arranged by size:
2574 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 402 434 2665 2695 2697 2727 351
Size: 32 19 32 2231 30 2 30 2447
Fragments arranged by size:
2447 2231 32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 1862 1862
Size: 4823
HindIII A'AGCT_T
Cuts at: 911 1163 1247 1415 1583 1751 911
Size: 252 84 168 168 168 3983
Fragments arranged by size:
3983 252 168 168 168 84
KasI G'GCGC_C
Cuts at: 2253 2501 2253
Size: 248 4575
KpnI G_GTAC'C
Cuts at: 1843 1843
Size: 4823
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3494 3678 3494
Size: 184 4639
MscI TGG'CCA
Cuts at: 229 462 2584 229
Size: 233 2122 2468
Fragments arranged by size:
2468 2122 233
MslI CAynn'nnrTG
Cuts at: 2812 2939 2812
Size: 127 4696
MspA1I CmG'CkG
Cuts at: 1934 2248 2608 3621 3866 1934
Size: 314 360 1013 245 2891
Fragments arranged by size:
2891 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 2254 2502 2254
Size: 248 4575
NcoI C'CATG_G
Cuts at: 107 107
Size: 4823
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NspI r_CATG'y
Cuts at: 409 756 1876 2376 3283 409
Size: 347 1120 500 907 1949
Fragments arranged by size:
1949 1120 907 500 347
PasI CC'CwG_GG
Cuts at: 160 160
Size: 4823
PciI A'CATG_T
Cuts at: 3279 3279
Size: 4823
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4791
PsiI TTA'TAA
Cuts at: 668 668
Size: 4823
PstI C_TGCA'G
Cuts at: 1870 2555 1870
Size: 685 4138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 2248 2608 2248
Size: 360 4463
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 1837 1837
Size: 4823
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4613
SbfI CC_TGCA'GG
Cuts at: 1870 1870
Size: 4823
ScaI AGT'ACT
Cuts at: 4340 4340
Size: 4823
SexAI A'CCwGG_T
Cuts at: 317 317
Size: 4823
SfcI C'TryA_G
Cuts at: 33 309 972 1056 1866 2551 3544 3735 33
Size: 276 663 84 810 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 663 276 191 84
SfoI GGC'GCC
Cuts at: 2255 2503 2255
Size: 248 4575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4823
SmlI C'TyrA_G
Cuts at: 139 1978 2893 3385 3647 139
Size: 1839 915 492 262 1315
Fragments arranged by size:
1839 1315 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 756 1876 2376 756
Size: 1120 500 3203
Fragments arranged by size:
3203 1120 500
SspI AAT'ATT
Cuts at: 635 635
Size: 4823
StyI C'CwwG_G
Cuts at: 107 1957 2311 107
Size: 1850 354 2619
Fragments arranged by size:
2619 1850 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2406 2539 2998 3207 2406
Size: 133 459 209 4022
Fragments arranged by size:
4022 459 209 133
TatI w'GTAC_w
Cuts at: 185 4338 185
Size: 4153 670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4340 4372 4340
Size: 32 4791
Tth111I GACn'n_nGTC
Cuts at: 2620 2620
Size: 4823
XbaI T'CTAG_A
Cuts at: 369 1854 369
Size: 1485 3338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 2318
Size: 4823
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4823
XmnI GAAnn'nnTTC
Cuts at: 635 718 635
Size: 83 4740
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI