Restriction map for
pF1KB7874
(Circular) (Six-base) MAPSORT of: pF1KB7874.seq Check: 6197 from: 1 to: 5024
pF1KB7874 5024 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 2062 2062
Size: 5024
Acc65I G'GTAC_C
Cuts at: 445 523 2040 445
Size: 78 1517 3429
Fragments arranged by size:
3429 1517 78
AclI AA'CG_TT
Cuts at: 4864 4864
Size: 5024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1019 2849 3281 4028 4296 1019
Size: 1830 432 747 268 1747
Fragments arranged by size:
1830 1747 747 432 268
AflIII A'CryG_T
Cuts at: 180 375 2376 3480 4457 180
Size: 195 2001 1104 977 747
Fragments arranged by size:
2001 1104 977 747 195
AgeI A'CCGG_T
Cuts at: 3412 3412
Size: 5024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1769 1808 1769
Size: 39 4985
AlwNI CAG_nnn'CTG
Cuts at: 709 1279 2329 3896 709
Size: 570 1050 1567 1837
Fragments arranged by size:
1837 1567 1050 570
ApaLI G'TGCA_C
Cuts at: 1141 3794 1141
Size: 2653 2371
ApoI r'AATT_y
Cuts at: 2028 4377 4633 2028
Size: 2349 256 2419
Fragments arranged by size:
2419 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5024
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5024
AvaI C'yCGr_G
Cuts at: 2044 2044
Size: 5024
BamHI G'GATC_C
Cuts at: 2049 2049
Size: 5024
BanI G'GyrC_C
Cuts at: 445 523 1847 1918 2040 2454 2702 2737 445
Size: 78 1324 71 122 414 248 35 2732
Fragments arranged by size:
2732 1324 414 248 122 78 71 35
BanII G_rGCy'C
Cuts at: 1439 2038 3068 1439
Size: 599 1030 3395
Fragments arranged by size:
3395 1030 599
BbeI G_GCGC'C
Cuts at: 2458 2706 2458
Size: 248 4776
BbsI GAAGACnn'nnnn_
Cuts at: 1381 1381
Size: 5024
BbvCI CC'TCA_GC
Cuts at: 1734 1769 1734
Size: 35 4989
BciVI GTATCCnnnnn_n'
Cuts at: 1235 2922 3683 5024 1235
Size: 1687 761 1341 1235
Fragments arranged by size:
1687 1341 1235 761
BclI T'GATC_A
Cuts at: 2544 2544
Size: 5024
BglII A'GATC_T
Cuts at: 1041 1388 2539 4576 1041
Size: 347 1151 2037 1489
Fragments arranged by size:
2037 1489 1151 347
BlpI GC'TnA_GC
Cuts at: 2136 2136
Size: 5024
Bme1580I G_kGCm'C
Cuts at: 1145 1850 2649 2742 3798 1145
Size: 705 799 93 1056 2371
Fragments arranged by size:
2371 1056 799 705 93
BmgBI CAC'GTC
Cuts at: 966 966
Size: 5024
BmrI ACTGGGnnnn_n'
Cuts at: 626 2236 2317 2652 4796 626
Size: 1610 81 335 2144 854
Fragments arranged by size:
2144 1610 854 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3391 3391
Size: 5024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 179
Size: 5024
Bpu10I CC'TnA_GC
Cuts at: 1734 1769 1734
Size: 35 4989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1625 1790 2200 3079 3571 3869 850
Size: 775 165 410 879 492 298 2005
Fragments arranged by size:
2005 879 775 492 410 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 4728 4728
Size: 5024
BsaAI yAC'GTr
Cuts at: 1517 2377 1517
Size: 860 4164
BsaBI GATnn'nnATC
Cuts at: 6 2538 4575 6
Size: 2532 2037 455
Fragments arranged by size:
2532 2037 455
BsaHI Gr'CG_yC
Cuts at: 2455 2703 3405 4901 2455
Size: 248 702 1496 2578
Fragments arranged by size:
2578 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2213 2734 3412 3686 3833 4244 2213
Size: 521 678 274 147 411 2993
Fragments arranged by size:
2993 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 4994
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 176 1459 1462 1722 176
Size: 1283 3 260 3478
Fragments arranged by size:
3478 1283 260 3
BseYI C'CCAG_C
Cuts at: 2449 3207 3784 4280 2449
Size: 758 577 496 3193
Fragments arranged by size:
3193 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1164 595
Size: 569 4455
BsiEI CG_ry'CG
Cuts at: 104 1110 2566 2612 3820 104
Size: 1006 1456 46 1208 1308
Fragments arranged by size:
1456 1308 1208 1006 46
BsiHKAI G_wGCw'C
Cuts at: 1145 2038 2816 3006 3798 1145
Size: 893 778 190 792 2371
Fragments arranged by size:
2371 893 792 778 190
BsmI GAATG_Cn'
Cuts at: 1819 1819
Size: 5024
Bsp1286I G_dGCh'C
Cuts at: 1145 1439 1850 2038 2649 2742 2816 3006 3068
Size: 294 411 188 611 93 74 190 62
Cuts at: 3068 3798 1145
Size: 730 2371
Fragments arranged by size:
2371 730 611 411 294 190 188 93
74 62
BspEI T'CCGG_A
Cuts at: 4244 4244
Size: 5024
BspHI T'CATG_A
Cuts at: 4200 4200
Size: 5024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 595 694 2074 2288 2590 2971 595
Size: 99 1380 214 302 381 2648
Fragments arranged by size:
2648 1380 381 302 214 99
BsrBI CCG'CTC
Cuts at: 50 3316 3370 4854 5019 50
Size: 3266 54 1484 165 55
Fragments arranged by size:
3266 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2936 2936
Size: 5024
BsrFI r'CCGG_y
Cuts at: 3022 3203 3412 3022
Size: 181 209 4634
Fragments arranged by size:
4634 209 181
BsrGI T'GTAC_A
Cuts at: 1270 1316 1270
Size: 46 4978
BssSI C'ACGA_G
Cuts at: 3295 3653 3295
Size: 358 4666
BstAPI GCAn_nnn'nTGC
Cuts at: 2401 2401
Size: 5024
BstBI TT'CG_AA
Cuts at: 1097 3385 1097
Size: 2288 2736
BstXI CCAn_nnnn'nTGG
Cuts at: 1677 1677
Size: 5024
BstYI r'GATC_y
Cuts at: 1 1041 1388 2049 2516 2539 2874 3120 4132
Size: 1040 347 661 467 23 335 246 1012
Cuts at: 4132 4218 4230 4576 1
Size: 86 12 346 449
Fragments arranged by size:
1040 1012 661 467 449 347 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 212 212
Size: 5024
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2974 3155 3318 2974
Size: 181 163 4680
Fragments arranged by size:
4680 181 163
BtsI GCAGTG_nn'
Cuts at: 554 2267 554
Size: 1713 3311
DraI TTT'AAA
Cuts at: 2023 2023
Size: 5024
DrdI GACnn_nn'nnGTC
Cuts at: 2730 3588 2730
Size: 858 4166
EaeI y'GGCC_r
Cuts at: 330 1400 2609 2783 3174 3201 4546 330
Size: 1070 1209 174 391 27 1345 808
Fragments arranged by size:
1345 1209 1070 808 391 174 27
EagI C'GGCC_G
Cuts at: 2609 2609
Size: 5024
EarI CTCTTCn'nnn_
Cuts at: 3047 3257 3047
Size: 210 4814
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3232 3542 3688 4834 3232
Size: 310 146 1146 3422
Fragments arranged by size:
3422 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2036 2036
Size: 5024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1019 2849 3281 4028 4296 179
Size: 840 1830 432 747 268 907
Fragments arranged by size:
1830 907 840 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 161 161
Size: 5024
EcoO109I rG'GnC_Cy
Cuts at: 1079 1505 2163 1079
Size: 426 658 3940
Fragments arranged by size:
3940 658 426
EcoRI G'AATT_C
Cuts at: 2028 2028
Size: 5024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1110 1513 3043 1110
Size: 403 1530 3091
Fragments arranged by size:
3091 1530 403
FspI TGC'GCA
Cuts at: 2805 2805
Size: 5024
HaeII r_GCGC'y
Cuts at: 2458 2706 3728 4707 2458
Size: 248 1022 979 2775
Fragments arranged by size:
2775 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2866 2896 2898 2928 2866
Size: 30 2 30 4962
Fragments arranged by size:
4962 30 30 2
HincII GTy'rAC
Cuts at: 1591 1632 2063 1591
Size: 41 431 4552
Fragments arranged by size:
4552 431 41
HindIII A'AGCT_T
Cuts at: 252 252
Size: 5024
KasI G'GCGC_C
Cuts at: 2454 2702 2454
Size: 248 4776
KpnI G_GTAC'C
Cuts at: 449 527 2044 449
Size: 78 1517 3429
Fragments arranged by size:
3429 1517 78
MfeI C'AATT_G
Cuts at: 629 1796 629
Size: 1167 3857
MluI A'CGCG_T
Cuts at: 4457 4457
Size: 5024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 1991 2013 3695 3879 326
Size: 1665 22 1682 184 1471
Fragments arranged by size:
1682 1665 1471 184 22
MscI TGG'CCA
Cuts at: 332 1402 2785 332
Size: 1070 1383 2571
Fragments arranged by size:
2571 1383 1070
MslI CAynn'nnrTG
Cuts at: 816 996 1675 3013 3140 816
Size: 180 679 1338 127 2700
Fragments arranged by size:
2700 1338 679 180 127
MspA1I CmG'CkG
Cuts at: 513 2135 2449 2809 3822 4067 513
Size: 1622 314 360 1013 245 1470
Fragments arranged by size:
1622 1470 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3205 3205
Size: 5024
NarI GG'CG_CC
Cuts at: 2455 2703 2455
Size: 248 4776
NdeI CA'TA_TG
Cuts at: 1263 1263
Size: 5024
NgoMIV G'CCGG_C
Cuts at: 3203 3203
Size: 5024
NspI r_CATG'y
Cuts at: 184 379 769 919 1630 1653 2077 2577 3484
Size: 195 390 150 711 23 424 500 907
Cuts at: 3484 184
Size: 1724
Fragments arranged by size:
1724 907 711 500 424 390 195 150
23
PciI A'CATG_T
Cuts at: 180 375 3480 180
Size: 195 3105 1724
Fragments arranged by size:
3105 1724 195
PflMI CCAn_nnn'nTGG
Cuts at: 1195 1195
Size: 5024
PfoI T'CCnGG_A
Cuts at: 156 156
Size: 5024
PmeI GTTT'AAAC
Cuts at: 2023 2023
Size: 5024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 4163
Size: 32 4992
PpuMI rG'GwC_Cy
Cuts at: 1505 1505
Size: 5024
PsiI TTA'TAA
Cuts at: 1226 1226
Size: 5024
PstI C_TGCA'G
Cuts at: 2071 2756 2071
Size: 685 4339
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5024
PvuII CAG'CTG
Cuts at: 513 2449 2809 513
Size: 1936 360 2728
Fragments arranged by size:
2728 1936 360
RsrII CG'GwC_CG
Cuts at: 3219 3219
Size: 5024
SacI G_AGCT'C
Cuts at: 2038 2038
Size: 5024
SalI G'TCGA_C
Cuts at: 2061 2061
Size: 5024
SanDI GG'GwC_CC
Cuts at: 1505 1505
Size: 5024
SapI GCTCTTCn'nnn_
Cuts at: 3047 3257 3047
Size: 210 4814
SbfI CC_TGCA'GG
Cuts at: 2071 2071
Size: 5024
ScaI AGT'ACT
Cuts at: 4541 4541
Size: 5024
SfcI C'TryA_G
Cuts at: 33 1045 2067 2752 3745 3936 33
Size: 1012 1022 685 993 191 1121
Fragments arranged by size:
1121 1022 1012 993 685 191
SfoI GGC'GCC
Cuts at: 2456 2704 2456
Size: 248 4776
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5024
SmaI CCC'GGG
Cuts at: 2046 2046
Size: 5024
SmlI C'TyrA_G
Cuts at: 865 1640 1805 2179 3094 3586 3848 865
Size: 775 165 374 915 492 262 2041
Fragments arranged by size:
2041 915 775 492 374 262 165
SnaBI TAC'GTA
Cuts at: 1517 1517
Size: 5024
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5024
SphI G_CATG'C
Cuts at: 919 2077 2577 919
Size: 1158 500 3366
Fragments arranged by size:
3366 1158 500
SspI AAT'ATT
Cuts at: 1306 1306
Size: 5024
StyI C'CwwG_G
Cuts at: 2158 2512 2158
Size: 354 4670
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1100 1271 1936 2607 2740 3199 3408 1100
Size: 171 665 671 133 459 209 2716
Fragments arranged by size:
2716 671 665 459 209 171 133
TatI w'GTAC_w
Cuts at: 429 1015 1270 1316 4539 429
Size: 586 255 46 3223 914
Fragments arranged by size:
3223 914 586 255 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 454 486 1327 1359 4541 4573 454
Size: 32 841 32 3182 32 905
Fragments arranged by size:
3182 905 841 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1586 2821 1586
Size: 1235 3789
XbaI T'CTAG_A
Cuts at: 411 1981 2055 411
Size: 1570 74 3380
Fragments arranged by size:
3380 1570 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 2519 651
Size: 1868 3156
XmaI C'CCGG_G
Cuts at: 2044 2044
Size: 5024
XmnI GAAnn'nnTTC
Cuts at: 1493 1493
Size: 5024
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI StuI SwaI XhoI
ZraI