Restriction map for
pF1KB7873
(Circular) (Six-base) MAPSORT of: pF1KB7873.seq Check: 3611 from: 1 to: 4823
pF1KB7873 4823 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 192 973 1153 1162 192
Size: 781 180 9 3853
Fragments arranged by size:
3853 781 180 9
AccI GT'mk_AC
Cuts at: 1861 1861
Size: 4823
Acc65I G'GTAC_C
Cuts at: 1839 1839
Size: 4823
AclI AA'CG_TT
Cuts at: 4663 4663
Size: 4823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 763 1403 2648 3080 3827 4095 480
Size: 283 640 1245 432 747 268 1208
Fragments arranged by size:
1245 1208 747 640 432 283 268
AflIII A'CryG_T
Cuts at: 930 2175 3279 4256 930
Size: 1245 1104 977 1497
Fragments arranged by size:
1497 1245 1104 977
AgeI A'CCGG_T
Cuts at: 3211 3211
Size: 4823
AleI CACnn'nnGTG
Cuts at: 767 857 767
Size: 90 4733
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 854 886 854
Size: 32 4791
AlwNI CAG_nnn'CTG
Cuts at: 1154 1467 2128 3695 1154
Size: 313 661 1567 2282
Fragments arranged by size:
2282 1567 661 313
ApaI G_GGCC'C
Cuts at: 1398 1398
Size: 4823
ApaLI G'TGCA_C
Cuts at: 1120 3593 1120
Size: 2473 2350
ApoI r'AATT_y
Cuts at: 1827 4176 4432 1827
Size: 2349 256 2218
Fragments arranged by size:
2349 2218 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 214 1095 1843 214
Size: 881 748 3194
Fragments arranged by size:
3194 881 748
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 741 774 741
Size: 33 4790
BamHI G'GATC_C
Cuts at: 1848 1848
Size: 4823
BanI G'GyrC_C
Cuts at: 1062 1426 1585 1839 2253 2501 2536 1062
Size: 364 159 254 414 248 35 3349
Fragments arranged by size:
3349 414 364 254 248 159 35
BanII G_rGCy'C
Cuts at: 672 977 1398 1837 2867 672
Size: 305 421 439 1030 2628
Fragments arranged by size:
2628 1030 439 421 305
BbeI G_GCGC'C
Cuts at: 2257 2505 2257
Size: 248 4575
BbsI GAAGACnn'nnnn_
Cuts at: 1460 1460
Size: 4823
BbvCI CC'TCA_GC
Cuts at: 1634 1634
Size: 4823
BciVI GTATCCnnnnn_n'
Cuts at: 2721 3482 4823 2721
Size: 761 1341 2721
Fragments arranged by size:
2721 1341 761
BclI T'GATC_A
Cuts at: 1412 2343 1412
Size: 931 3892
BglI GCCn_nnn'nGGC
Cuts at: 1369 1540 1762 1369
Size: 171 222 4430
Fragments arranged by size:
4430 222 171
BglII A'GATC_T
Cuts at: 2338 4375 2338
Size: 2037 2786
BlpI GC'TnA_GC
Cuts at: 1935 1935
Size: 4823
Bme1580I G_kGCm'C
Cuts at: 790 880 1124 1209 1398 1431 2448 2541 3597
Size: 90 244 85 189 33 1017 93 1056
Cuts at: 3597 790
Size: 2016
Fragments arranged by size:
2016 1056 1017 244 189 93 90 85
33
BmrI ACTGGGnnnn_n'
Cuts at: 1517 2035 2116 2451 4595 1517
Size: 518 81 335 2144 1745
Fragments arranged by size:
2144 1745 518 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1293 3190 1293
Size: 1897 2926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1240 1738 1810 541
Size: 699 498 72 3554
Fragments arranged by size:
3554 699 498 72
Bpu10I CC'TnA_GC
Cuts at: 1634 1634
Size: 4823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1626 1999 2878 3370 3668 1288
Size: 338 373 879 492 298 2443
Fragments arranged by size:
2443 879 492 373 338 298
BsaI GGTCTCn'nnnn_
Cuts at: 821 4527 821
Size: 3706 1117
BsaAI yAC'GTr
Cuts at: 1023 1116 2176 1023
Size: 93 1060 3670
Fragments arranged by size:
3670 1060 93
BsaBI GATnn'nnATC
Cuts at: 6 2337 4374 6
Size: 2331 2037 455
Fragments arranged by size:
2331 2037 455
BsaHI Gr'CG_yC
Cuts at: 627 2254 2502 3204 4700 627
Size: 1627 248 702 1496 750
Fragments arranged by size:
1627 1496 750 702 248
BsaWI w'CCGG_w
Cuts at: 2012 2533 3211 3485 3632 4043 2012
Size: 521 678 274 147 411 2792
Fragments arranged by size:
2792 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 808 1246 1303 1753 808
Size: 438 57 450 3878
Fragments arranged by size:
3878 450 438 57
BseYI C'CCAG_C
Cuts at: 352 706 1359 1430 1493 2248 3006 3583 4079
Size: 354 653 71 63 755 758 577 496
Cuts at: 4079 352
Size: 1096
Fragments arranged by size:
1096 758 755 653 577 496 354 71
63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 313 521 822 1732 109
Size: 204 208 301 910 3200
Fragments arranged by size:
3200 910 301 208 204
BsiEI CG_ry'CG
Cuts at: 104 2365 2411 3619 104
Size: 2261 46 1208 1308
Fragments arranged by size:
2261 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 433 672 1124 1837 2615 2805 3597 433
Size: 239 452 713 778 190 792 1659
Fragments arranged by size:
1659 792 778 713 452 239 190
BsmI GAATG_Cn'
Cuts at: 598 598
Size: 4823
BsmBI CGTCTCn'nnnn_
Cuts at: 634 634
Size: 4823
Bsp1286I G_dGCh'C
Cuts at: 433 672 790 880 977 1124 1209 1398 1431
Size: 239 118 90 97 147 85 189 33
Cuts at: 1431 1837 2448 2541 2615 2805 2867 3597 433
Size: 406 611 93 74 190 62 730 1659
Fragments arranged by size:
1659 730 611 406 239 190 189 147
118 97 93 90 85 74 62 33
BspEI T'CCGG_A
Cuts at: 4043 4043
Size: 4823
BspHI T'CATG_A
Cuts at: 546 3999 546
Size: 3453 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 192 839 973 1153 1162 1425 1466 1873 2087
Size: 647 134 180 9 263 41 407 214
Cuts at: 2087 2389 2770 192
Size: 302 381 2245
Fragments arranged by size:
2245 647 407 381 302 263 214 180
134 41 9
BsrBI CCG'CTC
Cuts at: 50 643 899 3115 3169 4653 4818 50
Size: 593 256 2216 54 1484 165 55
Fragments arranged by size:
2216 1484 593 256 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 2735 1703
Size: 1032 3791
BsrFI r'CCGG_y
Cuts at: 1169 2821 3002 3211 1169
Size: 1652 181 209 2781
Fragments arranged by size:
2781 1652 209 181
BssSI C'ACGA_G
Cuts at: 3094 3452 3094
Size: 358 4465
BstAPI GCAn_nnn'nTGC
Cuts at: 1154 2200 1154
Size: 1046 3777
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstEII G'GTnAC_C
Cuts at: 132 679 1488 132
Size: 547 809 3467
Fragments arranged by size:
3467 809 547
BstXI CCAn_nnnn'nTGG
Cuts at: 360 1485 1752 360
Size: 1125 267 3431
Fragments arranged by size:
3431 1125 267
BstYI r'GATC_y
Cuts at: 1 1848 2315 2338 2673 2919 3931 4017 4029
Size: 1847 467 23 335 246 1012 86 12
Cuts at: 4029 4375 1
Size: 346 449
Fragments arranged by size:
1847 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 822 822
Size: 4823
BtgI C'CryG_G
Cuts at: 107 987 1209 1226 1568 107
Size: 880 222 17 342 3362
Fragments arranged by size:
3362 880 342 222 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1387 2773 2954 3117 1387
Size: 1386 181 163 3093
Fragments arranged by size:
3093 1386 181 163
BtsI GCAGTG_nn'
Cuts at: 389 515 1128 1209 1715 1757 2066 389
Size: 126 613 81 506 42 309 3146
Fragments arranged by size:
3146 613 506 309 126 81 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 603 638 603
Size: 35 4788
DraI TTT'AAA
Cuts at: 1822 1822
Size: 4823
DraIII CAC_nnn'GTG
Cuts at: 132 246 411 1116 1119 132
Size: 114 165 705 3 3836
Fragments arranged by size:
3836 705 165 114 3
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2529 3387 1800
Size: 729 858 3236
Fragments arranged by size:
3236 858 729
EaeI y'GGCC_r
Cuts at: 440 894 984 1187 1541 1682 1754 1763 2408
Size: 454 90 203 354 141 72 9 645
Cuts at: 2408 2582 2973 3000 4345 440
Size: 174 391 27 1345 918
Fragments arranged by size:
1345 918 645 454 391 354 203 174
141 90 72 27 9
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 739 1337 1661 2846 3056 739
Size: 598 324 1185 210 2506
Fragments arranged by size:
2506 1185 598 324 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1286 3031 3341 3487 4633 1286
Size: 1745 310 146 1146 1476
Fragments arranged by size:
1745 1476 1146 310 146
EcoICRI GAG'CTC
Cuts at: 670 1835 670
Size: 1165 3658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 541 763 1240 1403 1738 1810 2648 3080
Size: 61 222 477 163 335 72 838 432
Cuts at: 3080 3827 4095 480
Size: 747 268 1208
Fragments arranged by size:
1208 838 747 477 432 335 268 222
163 72 61
EcoNI CCTnn'n_nnAGG
Cuts at: 1330 1330
Size: 4823
EcoO109I rG'GnC_Cy
Cuts at: 468 956 1245 1394 1395 1464 1962 468
Size: 488 289 149 1 69 498 3329
Fragments arranged by size:
3329 498 488 289 149 69 1
EcoRI G'AATT_C
Cuts at: 1827 1827
Size: 4823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4823
FspI TGC'GCA
Cuts at: 937 1027 2604 937
Size: 90 1577 3156
Fragments arranged by size:
3156 1577 90
FspAI rTGC'GCAy
Cuts at: 937 1027 937
Size: 90 4733
HaeII r_GCGC'y
Cuts at: 1004 1580 2257 2505 3527 4506 1004
Size: 576 677 248 1022 979 1321
Fragments arranged by size:
1321 1022 979 677 576 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1340 1372 2665 2695 2697 2727 1340
Size: 32 1293 30 2 30 3436
Fragments arranged by size:
3436 1293 32 30 30 2
HincII GTy'rAC
Cuts at: 1862 1862
Size: 4823
HindIII A'AGCT_T
Cuts at: 187 187
Size: 4823
KasI G'GCGC_C
Cuts at: 2253 2501 2253
Size: 248 4575
KpnI G_GTAC'C
Cuts at: 1843 1843
Size: 4823
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 396 567 1784 3494 3678 396
Size: 171 1217 1710 184 1541
Fragments arranged by size:
1710 1541 1217 184 171
MscI TGG'CCA
Cuts at: 1189 1543 1684 1756 2584 1189
Size: 354 141 72 828 3428
Fragments arranged by size:
3428 828 354 141 72
MslI CAynn'nnrTG
Cuts at: 677 693 767 783 857 1483 1708 1750 2812
Size: 16 74 16 74 626 225 42 1062
Cuts at: 2812 2939 677
Size: 127 2561
Fragments arranged by size:
2561 1062 626 225 127 74 74 42
16 16
MspA1I CmG'CkG
Cuts at: 297 605 710 989 1716 1934 2248 2608 3621
Size: 308 105 279 727 218 314 360 1013
Cuts at: 3621 3866 297
Size: 245 1254
Fragments arranged by size:
1254 1013 727 360 314 308 279 245
218 105
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 2254 2502 2254
Size: 248 4575
NcoI C'CATG_G
Cuts at: 107 1209 1568 107
Size: 1102 359 3362
Fragments arranged by size:
3362 1102 359
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NspI r_CATG'y
Cuts at: 844 934 1876 2376 3283 844
Size: 90 942 500 907 2384
Fragments arranged by size:
2384 942 907 500 90
PasI CC'CwG_GG
Cuts at: 1467 1467
Size: 4823
PciI A'CATG_T
Cuts at: 930 3279 930
Size: 2349 2474
PflMI CCAn_nnn'nTGG
Cuts at: 411 411
Size: 4823
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PmlI CAC'GTG
Cuts at: 1023 1116 1023
Size: 93 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4791
PpuMI rG'GwC_Cy
Cuts at: 468 468
Size: 4823
PspOMI G'GGCC_C
Cuts at: 1394 1394
Size: 4823
PspXI vC'TCGA_Gb
Cuts at: 214 1095 214
Size: 881 3942
PstI C_TGCA'G
Cuts at: 836 1150 1284 1463 1870 2555 836
Size: 314 134 179 407 685 3104
Fragments arranged by size:
3104 685 407 314 179 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 297 710 1716 2248 2608 297
Size: 413 1006 532 360 2512
Fragments arranged by size:
2512 1006 532 413 360
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 672 1837 672
Size: 1165 3658
SacII CC_GC'GG
Cuts at: 990 990
Size: 4823
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 739 2846 3056 739
Size: 2107 210 2506
Fragments arranged by size:
2506 2107 210
SbfI CC_TGCA'GG
Cuts at: 1463 1870 1463
Size: 407 4416
ScaI AGT'ACT
Cuts at: 1093 4340 1093
Size: 3247 1576
SexAI A'CCwGG_T
Cuts at: 242 242
Size: 4823
SfcI C'TryA_G
Cuts at: 33 832 1146 1280 1459 1866 2551 3544 3735
Size: 799 314 134 179 407 685 993 191
Cuts at: 3735 33
Size: 1121
Fragments arranged by size:
1121 993 799 685 407 314 191 179
134
SfiI GGCCn_nnn'nGGCC
Cuts at: 1762 1762
Size: 4823
SfoI GGC'GCC
Cuts at: 2255 2503 2255
Size: 248 4575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4823
SmlI C'TyrA_G
Cuts at: 214 1095 1267 1641 1978 2893 3385 3647 214
Size: 881 172 374 337 915 492 262 1390
Fragments arranged by size:
1390 915 881 492 374 337 262 172
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 1876 2376 1876
Size: 500 4323
StuI AGG'CCT
Cuts at: 721 811 721
Size: 90 4733
StyI C'CwwG_G
Cuts at: 107 1184 1209 1568 1957 2311 107
Size: 1077 25 359 389 354 2619
Fragments arranged by size:
2619 1077 389 359 354 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 955 1317 1507 1603 2406 2539 2998 3207
Size: 607 362 190 96 803 133 459 209
Cuts at: 3207 348
Size: 1964
Fragments arranged by size:
1964 803 607 459 362 209 190 133
96
TatI w'GTAC_w
Cuts at: 1091 4338 1091
Size: 3247 1576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4340 4372 4340
Size: 32 4791
Tth111I GACn'n_nGTC
Cuts at: 2620 2620
Size: 4823
XbaI T'CTAG_A
Cuts at: 1854 1854
Size: 4823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 217 379 2318 114
Size: 103 162 1939 2619
Fragments arranged by size:
2619 1939 162 103
XhoI C'TCGA_G
Cuts at: 214 1095 214
Size: 881 3942
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4823
XmnI GAAnn'nnTTC
Cuts at: 1081 1081
Size: 4823
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BcgI
BfrBI BmgBI BmtI BsaXI BsiWI BsrGI BssHII BstZ17I
ClaI EcoRV FseI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PshAI PsiI PsrI SanDI
SgrAI SnaBI SrfI SspI SwaI ZraI