Restriction map for
pF1KB7867
(Circular) (Six-base) MAPSORT of: pF1KB7867.seq Check: 3781 from: 1 to: 4793
pF1KB7867 4793 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 342 667 1426 342
Size: 325 759 3709
Fragments arranged by size:
3709 759 325
AatII G_ACGT'C
Cuts at: 1742 1742
Size: 4793
AccI GT'mk_AC
Cuts at: 1831 1831
Size: 4793
Acc65I G'GTAC_C
Cuts at: 1809 1809
Size: 4793
AclI AA'CG_TT
Cuts at: 4633 4633
Size: 4793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3050 3797 4065 2618
Size: 432 747 268 3346
Fragments arranged by size:
3346 747 432 268
AfeI AGC'GCT
Cuts at: 1342 1342
Size: 4793
AflIII A'CryG_T
Cuts at: 227 2145 3249 4226 227
Size: 1918 1104 977 794
Fragments arranged by size:
1918 1104 977 794
AgeI A'CCGG_T
Cuts at: 3181 3181
Size: 4793
AlwNI CAG_nnn'CTG
Cuts at: 1185 1441 2098 3665 1185
Size: 256 657 1567 2313
Fragments arranged by size:
2313 1567 657 256
ApaI G_GGCC'C
Cuts at: 793 793
Size: 4793
ApaLI G'TGCA_C
Cuts at: 3563 3563
Size: 4793
ApoI r'AATT_y
Cuts at: 1797 4146 4402 1797
Size: 2349 256 2188
Fragments arranged by size:
2349 2188 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4793
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
AvaI C'yCGr_G
Cuts at: 1486 1591 1813 1486
Size: 105 222 4466
Fragments arranged by size:
4466 222 105
BamHI G'GATC_C
Cuts at: 167 1818 167
Size: 1651 3142
BanI G'GyrC_C
Cuts at: 354 645 1074 1635 1809 2223 2471 2506 354
Size: 291 429 561 174 414 248 35 2641
Fragments arranged by size:
2641 561 429 414 291 248 174 35
BanII G_rGCy'C
Cuts at: 473 793 1376 1807 2837 473
Size: 320 583 431 1030 2429
Fragments arranged by size:
2429 1030 583 431 320
BbeI G_GCGC'C
Cuts at: 649 1078 2227 2475 649
Size: 429 1149 248 2967
Fragments arranged by size:
2967 1149 429 248
BbsI GAAGACnn'nnnn_
Cuts at: 129 129
Size: 4793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 879 913 1195 1229 879
Size: 34 282 34 4443
Fragments arranged by size:
4443 282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2691 3452 4793 2691
Size: 761 1341 2691
Fragments arranged by size:
2691 1341 761
BclI T'GATC_A
Cuts at: 2313 2313
Size: 4793
BglI GCCn_nnn'nGGC
Cuts at: 757 1427 757
Size: 670 4123
BglII A'GATC_T
Cuts at: 2308 4345 2308
Size: 2037 2756
BlpI GC'TnA_GC
Cuts at: 1442 1696 1905 1442
Size: 254 209 4330
Fragments arranged by size:
4330 254 209
Bme1580I G_kGCm'C
Cuts at: 793 2418 2511 3567 793
Size: 1625 93 1056 2019
Fragments arranged by size:
2019 1625 1056 93
BmgBI CAC'GTC
Cuts at: 135 135
Size: 4793
BmrI ACTGGGnnnn_n'
Cuts at: 2005 2086 2421 4565 2005
Size: 81 335 2144 2233
Fragments arranged by size:
2233 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 993 3160 993
Size: 2167 2626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 752 1564 1646 379
Size: 373 812 82 3526
Fragments arranged by size:
3526 812 373 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1969 2848 3340 3638 1969
Size: 879 492 298 3124
Fragments arranged by size:
3124 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1348 4497 1348
Size: 3149 1644
BsaAI yAC'GTr
Cuts at: 230 1332 1779 2146 230
Size: 1102 447 367 2877
Fragments arranged by size:
2877 1102 447 367
BsaBI GATnn'nnATC
Cuts at: 6 2307 4344 6
Size: 2301 2037 455
Fragments arranged by size:
2301 2037 455
BsaHI Gr'CG_yC
Cuts at: 646 1075 1603 1739 2224 2472 3174 4670 646
Size: 429 528 136 485 248 702 1496 769
Fragments arranged by size:
1496 769 702 528 485 429 248 136
BsaWI w'CCGG_w
Cuts at: 237 1982 2503 3181 3455 3602 4013 237
Size: 1745 521 678 274 147 411 1017
Fragments arranged by size:
1745 1017 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 1087
Size: 30 4763
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 393 1072 1101 1108 1111 256
Size: 137 679 29 7 3 3938
Fragments arranged by size:
3938 679 137 29 7 3
BseYI C'CCAG_C
Cuts at: 649 982 1434 1570 2218 2976 3553 4049 649
Size: 333 452 136 648 758 577 496 1393
Fragments arranged by size:
1393 758 648 577 496 452 333 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 370
Size: 4793
BsiEI CG_ry'CG
Cuts at: 104 1454 2335 2381 3589 104
Size: 1350 881 46 1208 1308
Fragments arranged by size:
1350 1308 1208 881 46
BsiHKAI G_wGCw'C
Cuts at: 134 473 768 1807 2585 2775 3567 134
Size: 339 295 1039 778 190 792 1360
Fragments arranged by size:
1360 1039 792 778 339 295 190
Bsp1286I G_dGCh'C
Cuts at: 134 473 768 793 1376 1807 2418 2511 2585
Size: 339 295 25 583 431 611 93 74
Cuts at: 2585 2775 2837 3567 134
Size: 190 62 730 1360
Fragments arranged by size:
1360 730 611 583 431 339 295 190
93 74 62 25
BspEI T'CCGG_A
Cuts at: 4013 4013
Size: 4793
BspHI T'CATG_A
Cuts at: 3969 3969
Size: 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 342 626 667 1217 1426 1466 1692 1843 2057
Size: 284 41 550 209 40 226 151 214
Cuts at: 2057 2359 2740 342
Size: 302 381 2395
Fragments arranged by size:
2395 550 381 302 284 226 214 209
151 41 40
BsrBI CCG'CTC
Cuts at: 50 516 548 3085 3139 4623 4788 50
Size: 466 32 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 466 165 55 54 32
BsrDI GCAATG_nn'
Cuts at: 2705 2705
Size: 4793
BsrFI r'CCGG_y
Cuts at: 388 751 810 1135 1599 2791 2972 3181 388
Size: 363 59 325 464 1192 181 209 2000
Fragments arranged by size:
2000 1192 464 363 325 209 181 59
BssHII G'CGCG_C
Cuts at: 437 1396 437
Size: 959 3834
BssSI C'ACGA_G
Cuts at: 3064 3422 3064
Size: 358 4435
BstAPI GCAn_nnn'nTGC
Cuts at: 1185 1343 2170 1185
Size: 158 827 3808
Fragments arranged by size:
3808 827 158
BstBI TT'CG_AA
Cuts at: 3154 3154
Size: 4793
BstXI CCAn_nnnn'nTGG
Cuts at: 1422 1422
Size: 4793
BstYI r'GATC_y
Cuts at: 1 167 1818 2285 2308 2643 2889 3901 3987
Size: 166 1651 467 23 335 246 1012 86
Cuts at: 3987 3999 4345 1
Size: 12 346 449
Fragments arranged by size:
1651 1012 467 449 346 335 246 166
86 23 12
BtgI C'CryG_G
Cuts at: 107 1359 107
Size: 1252 3541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2743 2924 3087 2743
Size: 181 163 4449
Fragments arranged by size:
4449 181 163
BtsI GCAGTG_nn'
Cuts at: 2036 2036
Size: 4793
DraI TTT'AAA
Cuts at: 1038 1792 1038
Size: 754 4039
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2499 3357 1602
Size: 897 858 3038
Fragments arranged by size:
3038 897 858
EaeI y'GGCC_r
Cuts at: 633 749 1451 2378 2552 2943 2970 4315 633
Size: 116 702 927 174 391 27 1345 1111
Fragments arranged by size:
1345 1111 927 702 391 174 116 27
EagI C'GGCC_G
Cuts at: 1451 2378 1451
Size: 927 3866
EarI CTCTTCn'nnn_
Cuts at: 313 2816 3026 313
Size: 2503 210 2080
Fragments arranged by size:
2503 2080 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1666 3001 3311 3457 4603 1666
Size: 1335 310 146 1146 1856
Fragments arranged by size:
1856 1335 1146 310 146
EcoICRI GAG'CTC
Cuts at: 471 1805 471
Size: 1334 3459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 752 1564 1646 2618 3050 3797 4065 379
Size: 373 812 82 972 432 747 268 1107
Fragments arranged by size:
1107 972 812 747 432 373 268 82
EcoO109I rG'GnC_Cy
Cuts at: 789 790 1221 1932 789
Size: 1 431 711 3650
Fragments arranged by size:
3650 711 431 1
EcoRI G'AATT_C
Cuts at: 1797 1797
Size: 4793
EcoRV GAT'ATC
Cuts at: 313 313
Size: 4793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1185 1731 2812 1185
Size: 546 1081 3166
Fragments arranged by size:
3166 1081 546
FspI TGC'GCA
Cuts at: 1336 2574 1336
Size: 1238 3555
HaeII r_GCGC'y
Cuts at: 649 1014 1078 1344 1449 2227 2475 3497 4476
Size: 365 64 266 105 778 248 1022 979
Cuts at: 4476 649
Size: 966
Fragments arranged by size:
1022 979 966 778 365 266 248 105
64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 297 329 1087 1119 1123 1155 2635 2665 2667
Size: 32 758 32 4 32 1480 30 2
Cuts at: 2667 2697 297
Size: 30 2393
Fragments arranged by size:
2393 1480 758 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 1832 1832
Size: 4793
KasI G'GCGC_C
Cuts at: 645 1074 2223 2471 645
Size: 429 1149 248 2967
Fragments arranged by size:
2967 1149 429 248
KpnI G_GTAC'C
Cuts at: 1813 1813
Size: 4793
MluI A'CGCG_T
Cuts at: 4226 4226
Size: 4793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3464 3648 3464
Size: 184 4609
MscI TGG'CCA
Cuts at: 2554 2554
Size: 4793
MslI CAynn'nnrTG
Cuts at: 2782 2909 2782
Size: 127 4666
MspA1I CmG'CkG
Cuts at: 121 303 1441 1633 1904 2218 2578 3591 3836
Size: 182 1138 192 271 314 360 1013 245
Cuts at: 3836 121
Size: 1078
Fragments arranged by size:
1138 1078 1013 360 314 271 245 192
182
NaeI GCC'GGC
Cuts at: 812 1137 2974 812
Size: 325 1837 2631
Fragments arranged by size:
2631 1837 325
NarI GG'CG_CC
Cuts at: 646 1075 2224 2472 646
Size: 429 1149 248 2967
Fragments arranged by size:
2967 1149 429 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4793
NgoMIV G'CCGG_C
Cuts at: 810 1135 2972 810
Size: 325 1837 2631
Fragments arranged by size:
2631 1837 325
NspI r_CATG'y
Cuts at: 930 1846 2346 3253 930
Size: 916 500 907 2470
Fragments arranged by size:
2470 916 907 500
PciI A'CATG_T
Cuts at: 3249 3249
Size: 4793
PflMI CCAn_nnn'nTGG
Cuts at: 124 124
Size: 4793
PfoI T'CCnGG_A
Cuts at: 251 395 251
Size: 144 4649
PmeI GTTT'AAAC
Cuts at: 1792 1792
Size: 4793
PmlI CAC'GTG
Cuts at: 230 1332 230
Size: 1102 3691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4761
PpuMI rG'GwC_Cy
Cuts at: 1221 1221
Size: 4793
PspOMI G'GGCC_C
Cuts at: 789 789
Size: 4793
PstI C_TGCA'G
Cuts at: 623 1190 1689 1840 2525 623
Size: 567 499 151 685 2891
Fragments arranged by size:
2891 685 567 499 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4793
PvuII CAG'CTG
Cuts at: 121 303 2218 2578 121
Size: 182 1915 360 2336
Fragments arranged by size:
2336 1915 360 182
RsrII CG'GwC_CG
Cuts at: 2988 2988
Size: 4793
SacI G_AGCT'C
Cuts at: 473 1807 473
Size: 1334 3459
SalI G'TCGA_C
Cuts at: 1830 1830
Size: 4793
SanDI GG'GwC_CC
Cuts at: 1221 1221
Size: 4793
SapI GCTCTTCn'nnn_
Cuts at: 2816 3026 2816
Size: 210 4583
SbfI CC_TGCA'GG
Cuts at: 1840 1840
Size: 4793
ScaI AGT'ACT
Cuts at: 556 4310 556
Size: 3754 1039
SfcI C'TryA_G
Cuts at: 33 619 1186 1685 1836 2521 3514 3705 33
Size: 586 567 499 151 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 567 499 191 151
SfoI GGC'GCC
Cuts at: 647 1076 2225 2473 647
Size: 429 1149 248 2967
Fragments arranged by size:
2967 1149 429 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
SgrAI Cr'CCGG_yG
Cuts at: 1599 1599
Size: 4793
SmaI CCC'GGG
Cuts at: 1815 1815
Size: 4793
SmlI C'TyrA_G
Cuts at: 1948 2863 3355 3617 1948
Size: 915 492 262 3124
Fragments arranged by size:
3124 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4793
SphI G_CATG'C
Cuts at: 930 1846 2346 930
Size: 916 500 3377
Fragments arranged by size:
3377 916 500
StuI AGG'CCT
Cuts at: 1168 1168
Size: 4793
StyI C'CwwG_G
Cuts at: 107 1160 1927 2281 107
Size: 1053 767 354 2619
Fragments arranged by size:
2619 1053 767 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1653 2376 2509 2968 3177 1653
Size: 723 133 459 209 3269
Fragments arranged by size:
3269 723 459 209 133
TatI w'GTAC_w
Cuts at: 554 4308 554
Size: 3754 1039
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 4310 4342 878
Size: 32 3400 32 1329
Fragments arranged by size:
3400 1329 32 32
Tth111I GACn'n_nGTC
Cuts at: 2590 2590
Size: 4793
XbaI T'CTAG_A
Cuts at: 1824 1824
Size: 4793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 429 726 2288 342
Size: 87 297 1562 2847
Fragments arranged by size:
2847 1562 297 87
XmaI C'CCGG_G
Cuts at: 1813 1813
Size: 4793
ZraI GAC'GTC
Cuts at: 1740 1740
Size: 4793
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI