Restriction map for
pF1KB7865
(Circular) (Six-base) MAPSORT of: pF1KB7865.seq Check: 71 from: 1 to: 4784
pF1KB7865 4784 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 995 1822 995
Size: 827 3957
Acc65I G'GTAC_C
Cuts at: 1800 1800
Size: 4784
AclI AA'CG_TT
Cuts at: 4624 4624
Size: 4784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 3041 3788 4056 2609
Size: 432 747 268 3337
Fragments arranged by size:
3337 747 432 268
AflIII A'CryG_T
Cuts at: 1640 1742 2136 3240 4217 1640
Size: 102 394 1104 977 2207
Fragments arranged by size:
2207 1104 977 394 102
AgeI A'CCGG_T
Cuts at: 809 3172 809
Size: 2363 2421
AlwNI CAG_nnn'CTG
Cuts at: 714 1539 2089 3656 714
Size: 825 550 1567 1842
Fragments arranged by size:
1842 1567 825 550
ApaI G_GGCC'C
Cuts at: 827 827
Size: 4784
ApaLI G'TGCA_C
Cuts at: 3554 3554
Size: 4784
ApoI r'AATT_y
Cuts at: 1788 4137 4393 1788
Size: 2349 256 2179
Fragments arranged by size:
2349 2179 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4784
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
AvaI C'yCGr_G
Cuts at: 1804 1804
Size: 4784
BamHI G'GATC_C
Cuts at: 1809 1809
Size: 4784
BanI G'GyrC_C
Cuts at: 577 1800 2214 2462 2497 577
Size: 1223 414 248 35 2864
Fragments arranged by size:
2864 1223 414 248 35
BanII G_rGCy'C
Cuts at: 163 275 346 785 827 970 1579 1798 2828
Size: 112 71 439 42 143 609 219 1030
Cuts at: 2828 163
Size: 2119
Fragments arranged by size:
2119 1030 609 439 219 143 112 71
42
BbeI G_GCGC'C
Cuts at: 2218 2466 2218
Size: 248 4536
BbsI GAAGACnn'nnnn_
Cuts at: 282 595 1739 282
Size: 313 1144 3327
Fragments arranged by size:
3327 1144 313
BciVI GTATCCnnnnn_n'
Cuts at: 2682 3443 4784 2682
Size: 761 1341 2682
Fragments arranged by size:
2682 1341 761
BclI T'GATC_A
Cuts at: 479 2304 479
Size: 1825 2959
BglII A'GATC_T
Cuts at: 2299 4336 2299
Size: 2037 2747
BlpI GC'TnA_GC
Cuts at: 157 1896 157
Size: 1739 3045
Bme1580I G_kGCm'C
Cuts at: 580 827 2409 2502 3558 580
Size: 247 1582 93 1056 1806
Fragments arranged by size:
1806 1582 1056 247 93
BmgBI CAC'GTC
Cuts at: 125 125
Size: 4784
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1662 1996 2077 2412 4556 1336
Size: 326 334 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 334 326 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 661 3151 661
Size: 2490 2294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 256 1445 115
Size: 141 1189 3454
Fragments arranged by size:
3454 1189 141
Bpu10I CC'TnA_GC
Cuts at: 145 145
Size: 4784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1960 2839 3331 3629 367
Size: 1593 879 492 298 1522
Fragments arranged by size:
1593 1522 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4488 4488
Size: 4784
BsaAI yAC'GTr
Cuts at: 2137 2137
Size: 4784
BsaBI GATnn'nnATC
Cuts at: 6 2298 4335 6
Size: 2292 2037 455
Fragments arranged by size:
2292 2037 455
BsaHI Gr'CG_yC
Cuts at: 1055 2215 2463 3165 4661 1055
Size: 1160 248 702 1496 1178
Fragments arranged by size:
1496 1178 1160 702 248
BsaWI w'CCGG_w
Cuts at: 440 809 1973 2494 3172 3446 3593 4004 440
Size: 369 1164 521 678 274 147 411 1220
Fragments arranged by size:
1220 1164 678 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 1357 1387 328
Size: 30 999 30 3725
Fragments arranged by size:
3725 999 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 424 1039 1119 1124 1185 1282 1343 214
Size: 210 615 80 5 61 97 61 3655
Fragments arranged by size:
3655 615 210 97 80 61 61 5
BseYI C'CCAG_C
Cuts at: 560 833 874 1123 1263 1388 1532 1595 2209
Size: 273 41 249 140 125 144 63 614
Cuts at: 2209 2967 3544 4040 560
Size: 758 577 496 1304
Fragments arranged by size:
1304 758 614 577 496 273 249 144
140 125 63 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 1525
Size: 4784
BsiEI CG_ry'CG
Cuts at: 104 861 2326 2372 3580 104
Size: 757 1465 46 1208 1308
Fragments arranged by size:
1465 1308 1208 757 46
BsiHKAI G_wGCw'C
Cuts at: 163 1798 2576 2766 3558 163
Size: 1635 778 190 792 1389
Fragments arranged by size:
1635 1389 792 778 190
BsmI GAATG_Cn'
Cuts at: 913 1030 913
Size: 117 4667
BsmBI CGTCTCn'nnnn_
Cuts at: 232 232
Size: 4784
Bsp1286I G_dGCh'C
Cuts at: 163 275 346 580 785 827 970 1579 1798
Size: 112 71 234 205 42 143 609 219
Cuts at: 1798 2409 2502 2576 2766 2828 3558 163
Size: 611 93 74 190 62 730 1389
Fragments arranged by size:
1389 730 611 609 234 219 205 190
143 112 93 74 71 62 42
BspEI T'CCGG_A
Cuts at: 4004 4004
Size: 4784
BspHI T'CATG_A
Cuts at: 1409 3960 1409
Size: 2551 2233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1834 2048 2350 2731 1834
Size: 214 302 381 3887
Fragments arranged by size:
3887 381 302 214
BsrBI CCG'CTC
Cuts at: 50 607 3076 3130 4614 4779 50
Size: 557 2469 54 1484 165 55
Fragments arranged by size:
2469 1484 557 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1706 2696 1706
Size: 990 3794
BsrFI r'CCGG_y
Cuts at: 285 535 809 1495 2782 2963 3172 285
Size: 250 274 686 1287 181 209 1897
Fragments arranged by size:
1897 1287 686 274 250 209 181
BsrGI T'GTAC_A
Cuts at: 611 611
Size: 4784
BssSI C'ACGA_G
Cuts at: 3055 3413 3055
Size: 358 4426
BstAPI GCAn_nnn'nTGC
Cuts at: 2161 2161
Size: 4784
BstBI TT'CG_AA
Cuts at: 3145 3145
Size: 4784
BstEII G'GTnAC_C
Cuts at: 168 1230 168
Size: 1062 3722
BstXI CCAn_nnnn'nTGG
Cuts at: 676 754 676
Size: 78 4706
BstYI r'GATC_y
Cuts at: 1 641 1809 2276 2299 2634 2880 3892 3978
Size: 640 1168 467 23 335 246 1012 86
Cuts at: 3978 3990 4336 1
Size: 12 346 449
Fragments arranged by size:
1168 1012 640 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 747 957 1049 1101 1212 1226 107
Size: 640 210 92 52 111 14 3665
Fragments arranged by size:
3665 640 210 111 92 52 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2734 2915 3078 2734
Size: 181 163 4440
Fragments arranged by size:
4440 181 163
BtsI GCAGTG_nn'
Cuts at: 2027 2027
Size: 4784
DraI TTT'AAA
Cuts at: 1783 1783
Size: 4784
DraIII CAC_nnn'GTG
Cuts at: 1471 1471
Size: 4784
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2490 3348 1239
Size: 1251 858 2675
Fragments arranged by size:
2675 1251 858
EaeI y'GGCC_r
Cuts at: 309 1046 1493 2369 2543 2934 2961 4306 309
Size: 737 447 876 174 391 27 1345 787
Fragments arranged by size:
1345 876 787 737 447 391 174 27
EagI C'GGCC_G
Cuts at: 2369 2369
Size: 4784
EarI CTCTTCn'nnn_
Cuts at: 878 2807 3017 878
Size: 1929 210 2645
Fragments arranged by size:
2645 1929 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 433 445 2992 3302 3448 4594 433
Size: 12 2547 310 146 1146 623
Fragments arranged by size:
2547 1146 623 310 146 12
EcoICRI GAG'CTC
Cuts at: 161 1796 161
Size: 1635 3149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 256 1445 2609 3041 3788 4056 115
Size: 141 1189 1164 432 747 268 843
Fragments arranged by size:
1189 1164 843 747 432 268 141
EcoO109I rG'GnC_Cy
Cuts at: 456 823 1923 456
Size: 367 1100 3317
Fragments arranged by size:
3317 1100 367
EcoRI G'AATT_C
Cuts at: 1788 1788
Size: 4784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 342 2803 306
Size: 36 2461 2287
Fragments arranged by size:
2461 2287 36
FspI TGC'GCA
Cuts at: 2565 2565
Size: 4784
HaeII r_GCGC'y
Cuts at: 152 400 1226 2218 2466 3488 4467 152
Size: 248 826 992 248 1022 979 469
Fragments arranged by size:
1022 992 979 826 469 248 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 760 792 2626 2656 2658 2688 328
Size: 32 400 32 1834 30 2 30 2424
Fragments arranged by size:
2424 1834 400 32 32 30 30 2
HincII GTy'rAC
Cuts at: 739 1823 739
Size: 1084 3700
KasI G'GCGC_C
Cuts at: 2214 2462 2214
Size: 248 4536
KpnI G_GTAC'C
Cuts at: 1804 1804
Size: 4784
MfeI C'AATT_G
Cuts at: 1436 1436
Size: 4784
MluI A'CGCG_T
Cuts at: 4217 4217
Size: 4784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 672 995 1091 3455 3639 672
Size: 323 96 2364 184 1817
Fragments arranged by size:
2364 1817 323 184 96
MscI TGG'CCA
Cuts at: 1048 2545 1048
Size: 1497 3287
MslI CAynn'nnrTG
Cuts at: 583 1741 2773 2900 583
Size: 1158 1032 127 2467
Fragments arranged by size:
2467 1158 1032 127
MspA1I CmG'CkG
Cuts at: 263 426 711 1539 1615 1626 1895 2209 2569
Size: 163 285 828 76 11 269 314 360
Cuts at: 2569 3582 3827 263
Size: 1013 245 1220
Fragments arranged by size:
1220 1013 828 360 314 285 269 245
163 76 11
NaeI GCC'GGC
Cuts at: 1497 2965 1497
Size: 1468 3316
NarI GG'CG_CC
Cuts at: 2215 2463 2215
Size: 248 4536
NcoI C'CATG_G
Cuts at: 107 747 1049 1212 1226 107
Size: 640 302 163 14 3665
Fragments arranged by size:
3665 640 302 163 14
NgoMIV G'CCGG_C
Cuts at: 1495 2963 1495
Size: 1468 3316
NspI r_CATG'y
Cuts at: 495 1644 1746 1837 2337 3244 495
Size: 1149 102 91 500 907 2035
Fragments arranged by size:
2035 1149 907 500 102 91
PciI A'CATG_T
Cuts at: 1640 1742 3240 1640
Size: 102 1498 3184
Fragments arranged by size:
3184 1498 102
PflMI CCAn_nnn'nTGG
Cuts at: 963 963
Size: 4784
PmeI GTTT'AAAC
Cuts at: 1783 1783
Size: 4784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4752
PpuMI rG'GwC_Cy
Cuts at: 456 456
Size: 4784
PspOMI G'GGCC_C
Cuts at: 823 823
Size: 4784
PstI C_TGCA'G
Cuts at: 395 1517 1631 1831 2516 395
Size: 1122 114 200 685 2663
Fragments arranged by size:
2663 1122 685 200 114
PvuI CG_AT'CG
Cuts at: 104 861 104
Size: 757 4027
PvuII CAG'CTG
Cuts at: 711 1539 1615 1626 2209 2569 711
Size: 828 76 11 583 360 2926
Fragments arranged by size:
2926 828 583 360 76 11
RsrII CG'GwC_CG
Cuts at: 2979 2979
Size: 4784
SacI G_AGCT'C
Cuts at: 163 1798 163
Size: 1635 3149
SalI G'TCGA_C
Cuts at: 1821 1821
Size: 4784
SapI GCTCTTCn'nnn_
Cuts at: 2807 3017 2807
Size: 210 4574
SbfI CC_TGCA'GG
Cuts at: 1831 1831
Size: 4784
ScaI AGT'ACT
Cuts at: 4301 4301
Size: 4784
SexAI A'CCwGG_T
Cuts at: 616 616
Size: 4784
SfcI C'TryA_G
Cuts at: 33 391 1059 1161 1513 1627 1827 2512 3505
Size: 358 668 102 352 114 200 685 993
Cuts at: 3505 3696 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 668 358 352 200 191
114 102
SfoI GGC'GCC
Cuts at: 2216 2464 2216
Size: 248 4536
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
SmaI CCC'GGG
Cuts at: 1806 1806
Size: 4784
SmlI C'TyrA_G
Cuts at: 382 1939 2854 3346 3608 382
Size: 1557 915 492 262 1558
Fragments arranged by size:
1558 1557 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4784
SphI G_CATG'C
Cuts at: 1837 2337 1837
Size: 500 4284
StuI AGG'CCT
Cuts at: 193 853 895 193
Size: 660 42 4082
Fragments arranged by size:
4082 660 42
StyI C'CwwG_G
Cuts at: 107 173 194 317 460 747 1049 1212 1226
Size: 66 21 123 143 287 302 163 14
Cuts at: 1226 1918 2272 107
Size: 692 354 2619
Fragments arranged by size:
2619 692 354 302 287 163 143 123
66 21 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2367 2500 2959 3168 2367
Size: 133 459 209 3983
Fragments arranged by size:
3983 459 209 133
TatI w'GTAC_w
Cuts at: 611 1729 4299 611
Size: 1118 2570 1096
Fragments arranged by size:
2570 1118 1096
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 435 467 1357 1389 1457 1489 4301
Size: 32 41 32 890 32 68 32 2812
Cuts at: 4301 4333 362
Size: 32 813
Fragments arranged by size:
2812 890 813 68 41 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2581 2581
Size: 4784
XbaI T'CTAG_A
Cuts at: 1815 1815
Size: 4784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 180 2279 180
Size: 2099 2685
XmaI C'CCGG_G
Cuts at: 1804 1804
Size: 4784
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BsiWI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI