Restriction map for
pF1KB7864
(Circular) (Six-base) MAPSORT of: pF1KB7864.seq Check: 7664 from: 1 to: 5006
pF1KB7864 5006 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1044 1847 1943 1044
Size: 803 96 4107
Fragments arranged by size:
4107 803 96
AccI GT'mk_AC
Cuts at: 899 1141 2044 899
Size: 242 903 3861
Fragments arranged by size:
3861 903 242
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 4846 4846
Size: 5006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3263 4010 4278 2831
Size: 432 747 268 3559
Fragments arranged by size:
3559 747 432 268
AflIII A'CryG_T
Cuts at: 959 2358 3462 4439 959
Size: 1399 1104 977 1526
Fragments arranged by size:
1526 1399 1104 977
AgeI A'CCGG_T
Cuts at: 3394 3394
Size: 5006
AleI CACnn'nnGTG
Cuts at: 889 889
Size: 5006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1909 1909
Size: 5006
AlwNI CAG_nnn'CTG
Cuts at: 195 747 867 1225 1509 1747 1839 2311 3878
Size: 552 120 358 284 238 92 472 1567
Cuts at: 3878 195
Size: 1323
Fragments arranged by size:
1567 1323 552 472 358 284 238 120
92
ApaI G_GGCC'C
Cuts at: 212 730 974 1234 212
Size: 518 244 260 3984
Fragments arranged by size:
3984 518 260 244
ApaLI G'TGCA_C
Cuts at: 3776 3776
Size: 5006
ApoI r'AATT_y
Cuts at: 2010 4359 4615 2010
Size: 2349 256 2401
Fragments arranged by size:
2401 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 308 990 1627 2026 308
Size: 682 637 399 3288
Fragments arranged by size:
3288 682 637 399
BamHI G'GATC_C
Cuts at: 2031 2031
Size: 5006
BanI G'GyrC_C
Cuts at: 1396 2022 2436 2684 2719 1396
Size: 626 414 248 35 3683
Fragments arranged by size:
3683 626 414 248 35
BanII G_rGCy'C
Cuts at: 143 212 316 674 730 874 974 1181 1234
Size: 69 104 358 56 144 100 207 53
Cuts at: 1234 1603 1909 2020 3050 143
Size: 369 306 111 1030 2099
Fragments arranged by size:
2099 1030 369 358 306 207 144 111
104 100 69 56 53
BbeI G_GCGC'C
Cuts at: 2440 2688 2440
Size: 248 4758
BbsI GAAGACnn'nnnn_
Cuts at: 350 577 1725 350
Size: 227 1148 3631
Fragments arranged by size:
3631 1148 227
BbvCI CC'TCA_GC
Cuts at: 382 382
Size: 5006
BciVI GTATCCnnnnn_n'
Cuts at: 2904 3665 5006 2904
Size: 761 1341 2904
Fragments arranged by size:
2904 1341 761
BclI T'GATC_A
Cuts at: 2526 2526
Size: 5006
BfrBI ATG'CAT
Cuts at: 830 830
Size: 5006
BglI GCCn_nnn'nGGC
Cuts at: 294 1585 294
Size: 1291 3715
BglII A'GATC_T
Cuts at: 2521 4558 2521
Size: 2037 2969
BlpI GC'TnA_GC
Cuts at: 169 281 1589 1858 2118 169
Size: 112 1308 269 260 3057
Fragments arranged by size:
3057 1308 269 260 112
Bme1580I G_kGCm'C
Cuts at: 212 562 730 974 1234 2631 2724 3780 212
Size: 350 168 244 260 1397 93 1056 1438
Fragments arranged by size:
1438 1397 1056 350 260 244 168 93
BmgBI CAC'GTC
Cuts at: 1806 1806
Size: 5006
BmrI ACTGGGnnnn_n'
Cuts at: 205 1043 1632 2218 2299 2634 4778 205
Size: 838 589 586 81 335 2144 433
Fragments arranged by size:
2144 838 589 586 433 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1383 3373 1383
Size: 1990 3016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 178 245 394 1195 1740 1866 1899 172
Size: 6 67 149 801 545 126 33 3279
Fragments arranged by size:
3279 801 545 149 126 67 33 6
Bpu10I CC'TnA_GC
Cuts at: 382 1696 382
Size: 1314 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 2182 3061 3553 3851 1095
Size: 1087 879 492 298 2250
Fragments arranged by size:
2250 1087 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 1221 1957 4710 415
Size: 806 736 2753 711
Fragments arranged by size:
2753 806 736 711
BsaAI yAC'GTr
Cuts at: 962 2359 962
Size: 1397 3609
BsaBI GATnn'nnATC
Cuts at: 6 2520 4557 6
Size: 2514 2037 455
Fragments arranged by size:
2514 2037 455
BsaHI Gr'CG_yC
Cuts at: 2437 2685 3387 4883 2437
Size: 248 702 1496 2560
Fragments arranged by size:
2560 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2195 2716 3394 3668 3815 4226 2195
Size: 521 678 274 147 411 2975
Fragments arranged by size:
2975 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 427 457 699 729 427
Size: 30 242 30 4704
Fragments arranged by size:
4704 242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 802 823 406
Size: 396 21 4589
Fragments arranged by size:
4589 396 21
BseYI C'CCAG_C
Cuts at: 297 729 737 867 1180 1225 1502 1810 2431
Size: 432 8 130 313 45 277 308 621
Cuts at: 2431 3189 3766 4262 297
Size: 758 577 496 1041
Fragments arranged by size:
1041 758 621 577 496 432 313 308
277 130 45 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1495 1567 1249
Size: 246 72 4688
Fragments arranged by size:
4688 246 72
BsiEI CG_ry'CG
Cuts at: 104 267 2548 2594 3802 104
Size: 163 2281 46 1208 1308
Fragments arranged by size:
2281 1308 1208 163 46
BsiHKAI G_wGCw'C
Cuts at: 143 1909 2020 2798 2988 3780 143
Size: 1766 111 778 190 792 1369
Fragments arranged by size:
1766 1369 792 778 190 111
BsmI GAATG_Cn'
Cuts at: 832 832
Size: 5006
BsmBI CGTCTCn'nnnn_
Cuts at: 319 790 319
Size: 471 4535
Bsp1286I G_dGCh'C
Cuts at: 143 212 316 562 674 730 874 974 1181
Size: 69 104 246 112 56 144 100 207
Cuts at: 1181 1234 1603 1909 2020 2631 2724 2798 2988
Size: 53 369 306 111 611 93 74 190
Cuts at: 2988 3050 3780 143
Size: 62 730 1369
Fragments arranged by size:
1369 730 611 369 306 246 207 190
144 112 111 104 100 93 74 69
62 56 53
BspEI T'CCGG_A
Cuts at: 4226 4226
Size: 5006
BspHI T'CATG_A
Cuts at: 1340 4182 1340
Size: 2842 2164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 1005 1044 1719 1847 1943 2056 2270 2572
Size: 523 39 675 128 96 113 214 302
Cuts at: 2572 2953 482
Size: 381 2535
Fragments arranged by size:
2535 675 523 381 302 214 128 113
96 39
BsrBI CCG'CTC
Cuts at: 50 1009 1533 3298 3352 4836 5001 50
Size: 959 524 1765 54 1484 165 55
Fragments arranged by size:
1765 1484 959 524 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1926 2918 1926
Size: 992 4014
BsrFI r'CCGG_y
Cuts at: 917 1446 1779 1825 1845 1853 3004 3185 3394
Size: 529 333 46 20 8 1151 181 209
Cuts at: 3394 917
Size: 2529
Fragments arranged by size:
2529 1151 529 333 209 181 46 20
8
BsrGI T'GTAC_A
Cuts at: 593 593
Size: 5006
BssSI C'ACGA_G
Cuts at: 3277 3635 3277
Size: 358 4648
BstAPI GCAn_nnn'nTGC
Cuts at: 1671 2383 1671
Size: 712 4294
BstBI TT'CG_AA
Cuts at: 3367 3367
Size: 5006
BstXI CCAn_nnnn'nTGG
Cuts at: 297 1173 1890 1983 297
Size: 876 717 93 3320
Fragments arranged by size:
3320 876 717 93
BstYI r'GATC_y
Cuts at: 1 743 2031 2498 2521 2856 3102 4114 4200
Size: 742 1288 467 23 335 246 1012 86
Cuts at: 4200 4212 4558 1
Size: 12 346 449
Fragments arranged by size:
1288 1012 742 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 389 389
Size: 5006
BtgI C'CryG_G
Cuts at: 107 413 442 953 1059 1166 1583 107
Size: 306 29 511 106 107 417 3530
Fragments arranged by size:
3530 511 417 306 107 106 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2956 3137 3300 2956
Size: 181 163 4662
Fragments arranged by size:
4662 181 163
BtsI GCAGTG_nn'
Cuts at: 181 1261 2249 181
Size: 1080 988 2938
Fragments arranged by size:
2938 1080 988
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DraIII CAC_nnn'GTG
Cuts at: 199 1065 1158 1441 199
Size: 866 93 283 3764
Fragments arranged by size:
3764 866 283 93
DrdI GACnn_nn'nnGTC
Cuts at: 2712 3570 2712
Size: 858 4148
EaeI y'GGCC_r
Cuts at: 950 1577 1706 1923 2591 2765 3156 3183 4528
Size: 627 129 217 668 174 391 27 1345
Cuts at: 4528 950
Size: 1428
Fragments arranged by size:
1428 1345 668 627 391 217 174 129
27
EagI C'GGCC_G
Cuts at: 2591 2591
Size: 5006
EarI CTCTTCn'nnn_
Cuts at: 662 1995 3029 3239 662
Size: 1333 1034 210 2429
Fragments arranged by size:
2429 1333 1034 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 720 838 3214 3524 3670 4816 720
Size: 118 2376 310 146 1146 910
Fragments arranged by size:
2376 1146 910 310 146 118
EcoICRI GAG'CTC
Cuts at: 141 1907 2018 141
Size: 1766 111 3129
Fragments arranged by size:
3129 1766 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 178 245 394 1195 1740 1866 1899 2831
Size: 6 67 149 801 545 126 33 932
Cuts at: 2831 3263 4010 4278 172
Size: 432 747 268 900
Fragments arranged by size:
932 900 801 747 545 432 268 149
126 67 33 6
EcoNI CCTnn'n_nnAGG
Cuts at: 241 430 553 751 762 241
Size: 189 123 198 11 4485
Fragments arranged by size:
4485 198 189 123 11
EcoO109I rG'GnC_Cy
Cuts at: 208 209 232 438 726 971 994 1230 1231
Size: 1 23 206 288 245 23 236 1
Cuts at: 1231 1375 1818 2145 208
Size: 144 443 327 3069
Fragments arranged by size:
3069 443 327 288 245 236 206 144
23 23 1 1
EcoRI G'AATT_C
Cuts at: 2010 2010
Size: 5006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1749 3025 1749
Size: 1276 3730
FspI TGC'GCA
Cuts at: 2787 2787
Size: 5006
HaeII r_GCGC'y
Cuts at: 1703 1865 2440 2688 3710 4689 1703
Size: 162 575 248 1022 979 2020
Fragments arranged by size:
2020 1022 979 575 248 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1267 1299 2848 2878 2880 2910 1267
Size: 32 1549 30 2 30 3363
Fragments arranged by size:
3363 1549 32 30 30 2
HincII GTy'rAC
Cuts at: 2045 2045
Size: 5006
KasI G'GCGC_C
Cuts at: 2436 2684 2436
Size: 248 4758
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 899 1970 3677 3861 899
Size: 1071 1707 184 2044
Fragments arranged by size:
2044 1707 1071 184
MscI TGG'CCA
Cuts at: 952 1579 1708 1925 2767 952
Size: 627 129 217 842 3191
Fragments arranged by size:
3191 842 627 217 129
MslI CAynn'nnrTG
Cuts at: 565 889 1981 2995 3122 565
Size: 324 1092 1014 127 2449
Fragments arranged by size:
2449 1092 1014 324 127
MspA1I CmG'CkG
Cuts at: 129 498 968 1118 1225 1509 1593 2117 2431
Size: 369 470 150 107 284 84 524 314
Cuts at: 2431 2791 3804 4049 129
Size: 360 1013 245 1086
Fragments arranged by size:
1086 1013 524 470 369 360 314 284
245 150 107 84
NaeI GCC'GGC
Cuts at: 1781 1827 1847 3187 1781
Size: 46 20 1340 3600
Fragments arranged by size:
3600 1340 46 20
NarI GG'CG_CC
Cuts at: 2437 2685 2437
Size: 248 4758
NcoI C'CATG_G
Cuts at: 107 953 1583 107
Size: 846 630 3530
Fragments arranged by size:
3530 846 630
NgoMIV G'CCGG_C
Cuts at: 1779 1825 1845 3185 1779
Size: 46 20 1340 3600
Fragments arranged by size:
3600 1340 46 20
NsiI A_TGCA'T
Cuts at: 832 832
Size: 5006
NspI r_CATG'y
Cuts at: 2059 2559 3466 2059
Size: 500 907 3599
Fragments arranged by size:
3599 907 500
PasI CC'CwG_GG
Cuts at: 979 1371 979
Size: 392 4614
PciI A'CATG_T
Cuts at: 3462 3462
Size: 5006
PflMI CCAn_nnn'nTGG
Cuts at: 195 195
Size: 5006
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4974
PpuMI rG'GwC_Cy
Cuts at: 438 994 1375 1818 438
Size: 556 381 443 3626
Fragments arranged by size:
3626 556 443 381
PspOMI G'GGCC_C
Cuts at: 208 726 970 1230 208
Size: 518 244 260 3984
Fragments arranged by size:
3984 518 260 244
PstI C_TGCA'G
Cuts at: 134 434 479 1101 1598 1844 2053 2738 134
Size: 300 45 622 497 246 209 685 2402
Fragments arranged by size:
2402 685 622 497 300 246 209 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 129 1225 1593 2431 2791 129
Size: 1096 368 838 360 2344
Fragments arranged by size:
2344 1096 838 368 360
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 143 1909 2020 143
Size: 1766 111 3129
Fragments arranged by size:
3129 1766 111
SalI G'TCGA_C
Cuts at: 2043 2043
Size: 5006
SapI GCTCTTCn'nnn_
Cuts at: 662 3029 3239 662
Size: 2367 210 2429
Fragments arranged by size:
2429 2367 210
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SexAI A'CCwGG_T
Cuts at: 598 598
Size: 5006
SfcI C'TryA_G
Cuts at: 33 130 430 475 1097 1594 1614 1840 2049
Size: 97 300 45 622 497 20 226 209
Cuts at: 2049 2734 3727 3918 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 622 497 300 226 209
191 97 45 20
SfoI GGC'GCC
Cuts at: 2438 2686 2438
Size: 248 4758
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 992 2028 992
Size: 1036 3970
SmlI C'TyrA_G
Cuts at: 1110 2161 3076 3568 3830 1110
Size: 1051 915 492 262 2286
Fragments arranged by size:
2286 1051 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 2059 2559 2059
Size: 500 4506
StuI AGG'CCT
Cuts at: 757 1292 1753 757
Size: 535 461 4010
Fragments arranged by size:
4010 535 461
StyI C'CwwG_G
Cuts at: 107 953 1583 2140 2494 107
Size: 846 630 557 354 2619
Fragments arranged by size:
2619 846 630 557 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 372 655 1660 1993 2589 2722 3181 3390
Size: 185 283 1005 333 596 133 459 209
Cuts at: 3390 187
Size: 1803
Fragments arranged by size:
1803 1005 596 459 333 283 209 185
133
TatI w'GTAC_w
Cuts at: 593 1198 1640 4521 593
Size: 605 442 2881 1078
Fragments arranged by size:
2881 1078 605 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 427 459 1144 1176 4523 4555 427
Size: 32 685 32 3347 32 878
Fragments arranged by size:
3347 878 685 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1137 2803 1137
Size: 1666 3340
XbaI T'CTAG_A
Cuts at: 2037 2037
Size: 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2501
Size: 5006
XmaI C'CCGG_G
Cuts at: 990 2026 990
Size: 1036 3970
XmnI GAAnn'nnTTC
Cuts at: 715 1570 715
Size: 855 4151
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BmtI BsiWI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI