Restriction map for
pF1KB7860
(Circular) (Six-base) MAPSORT of: pF1KB7860.seq Check: 2266 from: 1 to: 4910
pF1KB7860 4910 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1289 1289
Size: 4910
AccI GT'mk_AC
Cuts at: 1948 1948
Size: 4910
Acc65I G'GTAC_C
Cuts at: 1926 1926
Size: 4910
AclI AA'CG_TT
Cuts at: 4750 4750
Size: 4910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 263 853 914 1418 1630 1659 1786 2735
Size: 100 590 61 504 212 29 127 949
Cuts at: 2735 3167 3914 4182 163
Size: 432 747 268 891
Fragments arranged by size:
949 891 747 590 504 432 268 212
127 100 61 29
AflIII A'CryG_T
Cuts at: 2262 3366 4343 2262
Size: 1104 977 2829
Fragments arranged by size:
2829 1104 977
AgeI A'CCGG_T
Cuts at: 3298 3298
Size: 4910
AleI CACnn'nnGTG
Cuts at: 1550 1550
Size: 4910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 4910
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1207 1239 1207
Size: 32 4878
AlwNI CAG_nnn'CTG
Cuts at: 2215 3782 2215
Size: 1567 3343
ApaLI G'TGCA_C
Cuts at: 1870 3680 1870
Size: 1810 3100
ApoI r'AATT_y
Cuts at: 549 700 727 997 1504 1914 4263 4519 549
Size: 151 27 270 507 410 2349 256 940
Fragments arranged by size:
2349 940 507 410 270 256 151 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4910
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
AvaI C'yCGr_G
Cuts at: 152 1930 152
Size: 1778 3132
BamHI G'GATC_C
Cuts at: 1935 1935
Size: 4910
BanI G'GyrC_C
Cuts at: 369 1926 2340 2588 2623 369
Size: 1557 414 248 35 2656
Fragments arranged by size:
2656 1557 414 248 35
BanII G_rGCy'C
Cuts at: 176 1924 2954 176
Size: 1748 1030 2132
Fragments arranged by size:
2132 1748 1030
BbeI G_GCGC'C
Cuts at: 2344 2592 2344
Size: 248 4662
BbsI GAAGACnn'nnnn_
Cuts at: 251 564 1136 1207 251
Size: 313 572 71 3954
Fragments arranged by size:
3954 572 313 71
BbvCI CC'TCA_GC
Cuts at: 497 497
Size: 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4876
BciVI GTATCCnnnnn_n'
Cuts at: 1341 2808 3569 4910 1341
Size: 1467 761 1341 1341
Fragments arranged by size:
1467 1341 1341 761
BclI T'GATC_A
Cuts at: 1031 1095 2430 1031
Size: 64 1335 3511
Fragments arranged by size:
3511 1335 64
BglII A'GATC_T
Cuts at: 2425 4462 2425
Size: 2037 2873
BlpI GC'TnA_GC
Cuts at: 656 2022 656
Size: 1366 3544
Bme1580I G_kGCm'C
Cuts at: 1874 2535 2628 3684 1874
Size: 661 93 1056 3100
Fragments arranged by size:
3100 1056 661 93
BmrI ACTGGGnnnn_n'
Cuts at: 727 2122 2203 2538 4682 727
Size: 1395 81 335 2144 955
Fragments arranged by size:
2144 1395 955 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 4910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 649 899 1739 245
Size: 404 250 840 3416
Fragments arranged by size:
3416 840 404 250
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 4910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2086 2965 3457 3755 2086
Size: 879 492 298 3241
Fragments arranged by size:
3241 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1212 4614 1212
Size: 3402 1508
BsaAI yAC'GTr
Cuts at: 123 2263 123
Size: 2140 2770
BsaBI GATnn'nnATC
Cuts at: 6 2424 4461 6
Size: 2418 2037 455
Fragments arranged by size:
2418 2037 455
BsaHI Gr'CG_yC
Cuts at: 2341 2589 3291 4787 2341
Size: 248 702 1496 2464
Fragments arranged by size:
2464 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1538 2099 2620 3298 3572 3719 4130 1253
Size: 285 561 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 561 521 411 285 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 1207 1237 1630 1660 121
Size: 30 1056 30 393 30 3371
Fragments arranged by size:
3371 1056 393 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 514 706 1228 514
Size: 192 522 4196
Fragments arranged by size:
4196 522 192
BseYI C'CCAG_C
Cuts at: 111 2335 3093 3670 4166 111
Size: 2224 758 577 496 855
Fragments arranged by size:
2224 855 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1683 1683
Size: 4910
BsiEI CG_ry'CG
Cuts at: 104 2452 2498 3706 104
Size: 2348 46 1208 1308
Fragments arranged by size:
2348 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1110 1657 1703 1874 1924 2702 2892 3684 1110
Size: 547 46 171 50 778 190 792 2336
Fragments arranged by size:
2336 792 778 547 190 171 50 46
BsmI GAATG_Cn'
Cuts at: 242 1828 242
Size: 1586 3324
Bsp1286I G_dGCh'C
Cuts at: 176 1110 1657 1703 1874 1924 2535 2628 2702
Size: 934 547 46 171 50 611 93 74
Cuts at: 2702 2892 2954 3684 176
Size: 190 62 730 1402
Fragments arranged by size:
1402 934 730 611 547 190 171 93
74 62 50 46
BspEI T'CCGG_A
Cuts at: 1253 1538 4130 1253
Size: 285 2592 2033
Fragments arranged by size:
2592 2033 285
BspHI T'CATG_A
Cuts at: 363 963 1377 4086 363
Size: 600 414 2709 1187
Fragments arranged by size:
2709 1187 600 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1289 1960 2174 2476 2857 1289
Size: 671 214 302 381 3342
Fragments arranged by size:
3342 671 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3202 3256 4740 4905 50
Size: 3152 54 1484 165 55
Fragments arranged by size:
3152 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 670 2822 670
Size: 2152 2758
BsrFI r'CCGG_y
Cuts at: 2908 3089 3298 2908
Size: 181 209 4520
Fragments arranged by size:
4520 209 181
BsrGI T'GTAC_A
Cuts at: 311 311
Size: 4910
BssSI C'ACGA_G
Cuts at: 3181 3539 3181
Size: 358 4552
BstAPI GCAn_nnn'nTGC
Cuts at: 747 2287 747
Size: 1540 3370
BstBI TT'CG_AA
Cuts at: 3271 3271
Size: 4910
BstYI r'GATC_y
Cuts at: 1 882 1935 2402 2425 2760 3006 4018 4104
Size: 881 1053 467 23 335 246 1012 86
Cuts at: 4104 4116 4462 1
Size: 12 346 449
Fragments arranged by size:
1053 1012 881 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 334 1887 334
Size: 1553 3357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1340 2860 3041 3204 1340
Size: 1520 181 163 3046
Fragments arranged by size:
3046 1520 181 163
BtsI GCAGTG_nn'
Cuts at: 1320 1521 2153 1320
Size: 201 632 4077
Fragments arranged by size:
4077 632 201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 699 734 1137 1172 699
Size: 35 403 35 4437
Fragments arranged by size:
4437 403 35 35
DraI TTT'AAA
Cuts at: 1909 1909
Size: 4910
DrdI GACnn_nn'nnGTC
Cuts at: 2616 3474 2616
Size: 858 4052
EaeI y'GGCC_r
Cuts at: 1497 2495 2669 3060 3087 4432 1497
Size: 998 174 391 27 1345 1975
Fragments arranged by size:
1975 1345 998 391 174 27
EagI C'GGCC_G
Cuts at: 2495 2495
Size: 4910
EarI CTCTTCn'nnn_
Cuts at: 226 829 2933 3143 226
Size: 603 2104 210 1993
Fragments arranged by size:
2104 1993 603 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3118 3428 3574 4720 3118
Size: 310 146 1146 3308
Fragments arranged by size:
3308 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1922 1922
Size: 4910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 245 263 649 853 899 914 1418 1630
Size: 82 18 386 204 46 15 504 212
Cuts at: 1630 1659 1739 1786 2735 3167 3914 4182 163
Size: 29 80 47 949 432 747 268 891
Fragments arranged by size:
949 891 747 504 432 386 268 212
204 82 80 47 46 29 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1338 1338
Size: 4910
EcoO109I rG'GnC_Cy
Cuts at: 2049 2049
Size: 4910
EcoRI G'AATT_C
Cuts at: 1914 1914
Size: 4910
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 604 2929 604
Size: 2325 2585
FspI TGC'GCA
Cuts at: 2691 2691
Size: 4910
HaeII r_GCGC'y
Cuts at: 2344 2592 3614 4593 2344
Size: 248 1022 979 2661
Fragments arranged by size:
2661 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 358 390 1080 1112 1520 1552 1785
Size: 32 205 32 690 32 408 32 233
Cuts at: 1785 1817 2752 2782 2784 2814 121
Size: 32 935 30 2 30 2217
Fragments arranged by size:
2217 935 690 408 233 205 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1949 1949
Size: 4910
HindIII A'AGCT_T
Cuts at: 895 895
Size: 4910
KasI G'GCGC_C
Cuts at: 2340 2588 2340
Size: 248 4662
KpnI G_GTAC'C
Cuts at: 1930 1930
Size: 4910
MluI A'CGCG_T
Cuts at: 4343 4343
Size: 4910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 3581 3765 368
Size: 3213 184 1513
Fragments arranged by size:
3213 1513 184
MscI TGG'CCA
Cuts at: 1499 2671 1499
Size: 1172 3738
MslI CAynn'nnrTG
Cuts at: 121 1178 1295 1325 1550 2899 3026 121
Size: 1057 117 30 225 1349 127 2005
Fragments arranged by size:
2005 1349 1057 225 127 117 30
MspA1I CmG'CkG
Cuts at: 571 818 2021 2335 2695 3708 3953 571
Size: 247 1203 314 360 1013 245 1528
Fragments arranged by size:
1528 1203 1013 360 314 247 245
NaeI GCC'GGC
Cuts at: 3091 3091
Size: 4910
NarI GG'CG_CC
Cuts at: 2341 2589 2341
Size: 248 4662
NcoI C'CATG_G
Cuts at: 1887 1887
Size: 4910
NdeI CA'TA_TG
Cuts at: 1028 1028
Size: 4910
NgoMIV G'CCGG_C
Cuts at: 3089 3089
Size: 4910
NspI r_CATG'y
Cuts at: 120 1963 2463 3370 120
Size: 1843 500 907 1660
Fragments arranged by size:
1843 1660 907 500
PciI A'CATG_T
Cuts at: 3366 3366
Size: 4910
PflMI CCAn_nnn'nTGG
Cuts at: 1495 1495
Size: 4910
PmeI GTTT'AAAC
Cuts at: 1909 1909
Size: 4910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1207 1239 4049 4081 1207
Size: 32 2810 32 2036
Fragments arranged by size:
2810 2036 32 32
PstI C_TGCA'G
Cuts at: 1957 2642 1957
Size: 685 4225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4910
PvuII CAG'CTG
Cuts at: 818 2335 2695 818
Size: 1517 360 3033
Fragments arranged by size:
3033 1517 360
RsrII CG'GwC_CG
Cuts at: 3105 3105
Size: 4910
SacI G_AGCT'C
Cuts at: 1924 1924
Size: 4910
SalI G'TCGA_C
Cuts at: 1947 1947
Size: 4910
SapI GCTCTTCn'nnn_
Cuts at: 829 2933 3143 829
Size: 2104 210 2596
Fragments arranged by size:
2596 2104 210
SbfI CC_TGCA'GG
Cuts at: 1957 1957
Size: 4910
ScaI AGT'ACT
Cuts at: 4427 4427
Size: 4910
SfcI C'TryA_G
Cuts at: 33 943 1953 2638 3631 3822 33
Size: 910 1010 685 993 191 1121
Fragments arranged by size:
1121 1010 993 910 685 191
SfoI GGC'GCC
Cuts at: 2342 2590 2342
Size: 248 4662
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
SmaI CCC'GGG
Cuts at: 154 1932 154
Size: 1778 3132
SmlI C'TyrA_G
Cuts at: 2065 2980 3472 3734 2065
Size: 915 492 262 3241
Fragments arranged by size:
3241 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4910
SphI G_CATG'C
Cuts at: 1963 2463 1963
Size: 500 4410
StuI AGG'CCT
Cuts at: 1420 1672 1691 1420
Size: 252 19 4639
Fragments arranged by size:
4639 252 19
StyI C'CwwG_G
Cuts at: 588 1887 2044 2398 588
Size: 1299 157 354 3100
Fragments arranged by size:
3100 1299 354 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1051 1093 2493 2626 3085 3294 1051
Size: 42 1400 133 459 209 2667
Fragments arranged by size:
2667 1400 459 209 133 42
TatI w'GTAC_w
Cuts at: 311 4425 311
Size: 4114 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 867 899 4427 4459 867
Size: 32 3528 32 1318
Fragments arranged by size:
3528 1318 32 32
Tth111I GACn'n_nGTC
Cuts at: 2707 2707
Size: 4910
XbaI T'CTAG_A
Cuts at: 553 1941 553
Size: 1388 3522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1812 2405 1812
Size: 593 4317
XmaI C'CCGG_G
Cuts at: 152 1930 152
Size: 1778 3132
XmnI GAAnn'nnTTC
Cuts at: 727 727
Size: 4910
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI