Restriction map for
pF1KB7856
(Circular) (Six-base) MAPSORT of: pF1KB7856.seq Check: 6455 from: 1 to: 4994
pF1KB7856 4994 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 460 2032 460
Size: 1572 3422
Acc65I G'GTAC_C
Cuts at: 2010 2010
Size: 4994
AclI AA'CG_TT
Cuts at: 1976 4834 1976
Size: 2858 2136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 643 878 2819 3251 3998 4266 528
Size: 115 235 1941 432 747 268 1256
Fragments arranged by size:
1941 1256 747 432 268 235 115
AflIII A'CryG_T
Cuts at: 566 777 783 824 910 2346 3450 4427 566
Size: 211 6 41 86 1436 1104 977 1133
Fragments arranged by size:
1436 1133 1104 977 211 86 41 6
AgeI A'CCGG_T
Cuts at: 3382 3382
Size: 4994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 686 686
Size: 4994
AlwNI CAG_nnn'CTG
Cuts at: 456 2299 3866 456
Size: 1843 1567 1584
Fragments arranged by size:
1843 1584 1567
ApaLI G'TGCA_C
Cuts at: 3764 3764
Size: 4994
ApoI r'AATT_y
Cuts at: 968 1136 1447 1892 1936 1998 4347 4603 968
Size: 168 311 445 44 62 2349 256 1359
Fragments arranged by size:
2349 1359 445 311 256 168 62 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4994
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
AvaI C'yCGr_G
Cuts at: 2014 2014
Size: 4994
BamHI G'GATC_C
Cuts at: 2019 2019
Size: 4994
BanI G'GyrC_C
Cuts at: 294 2010 2424 2672 2707 294
Size: 1716 414 248 35 2581
Fragments arranged by size:
2581 1716 414 248 35
BanII G_rGCy'C
Cuts at: 946 1702 1870 2008 3038 946
Size: 756 168 138 1030 2902
Fragments arranged by size:
2902 1030 756 168 138
BbeI G_GCGC'C
Cuts at: 2428 2676 2428
Size: 248 4746
BbsI GAAGACnn'nnnn_
Cuts at: 187 376 187
Size: 189 4805
BciVI GTATCCnnnnn_n'
Cuts at: 2892 3653 4994 2892
Size: 761 1341 2892
Fragments arranged by size:
2892 1341 761
BclI T'GATC_A
Cuts at: 873 2514 873
Size: 1641 3353
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4994
BglII A'GATC_T
Cuts at: 817 1363 1405 2509 4546 817
Size: 546 42 1104 2037 1265
Fragments arranged by size:
2037 1265 1104 546 42
BlpI GC'TnA_GC
Cuts at: 2106 2106
Size: 4994
Bme1580I G_kGCm'C
Cuts at: 2619 2712 3768 2619
Size: 93 1056 3845
Fragments arranged by size:
3845 1056 93
BmgBI CAC'GTC
Cuts at: 162 162
Size: 4994
BmrI ACTGGGnnnn_n'
Cuts at: 391 753 2206 2287 2622 4766 391
Size: 362 1453 81 335 2144 619
Fragments arranged by size:
2144 1453 619 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 332 3361 332
Size: 3029 1965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 296 998 1082 1418 1586 1670 1686 259
Size: 37 702 84 336 168 84 16 3567
Fragments arranged by size:
3567 702 336 168 84 84 37 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 2170 3049 3541 3839 423
Size: 1747 879 492 298 1578
Fragments arranged by size:
1747 1578 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 794 4698 794
Size: 3904 1090
BsaAI yAC'GTr
Cuts at: 784 2347 784
Size: 1563 3431
BsaBI GATnn'nnATC
Cuts at: 6 2508 4545 6
Size: 2502 2037 455
Fragments arranged by size:
2502 2037 455
BsaHI Gr'CG_yC
Cuts at: 2425 2673 3375 4871 2425
Size: 248 702 1496 2548
Fragments arranged by size:
2548 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1298 1732 1816 2183 2704 3382 3656 3803 4214
Size: 434 84 367 521 678 274 147 411
Cuts at: 4214 1298
Size: 2078
Fragments arranged by size:
2078 678 521 434 411 367 274 147
84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 740 770 740
Size: 30 4964
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 304
Size: 4994
BseYI C'CCAG_C
Cuts at: 2419 3177 3754 4250 2419
Size: 758 577 496 3163
Fragments arranged by size:
3163 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 728 849 1101 1185 1269 1521 1773 567
Size: 161 121 252 84 84 252 252 3788
Fragments arranged by size:
3788 252 252 252 161 121 84 84
BsiEI CG_ry'CG
Cuts at: 104 119 2536 2582 3790 104
Size: 15 2417 46 1208 1308
Fragments arranged by size:
2417 1308 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 313 946 1702 1870 2008 2786 2976 3768 313
Size: 633 756 168 138 778 190 792 1539
Fragments arranged by size:
1539 792 778 756 633 190 168 138
BsmI GAATG_Cn'
Cuts at: 1840 1840
Size: 4994
Bsp1286I G_dGCh'C
Cuts at: 313 946 1702 1870 2008 2619 2712 2786 2976
Size: 633 756 168 138 611 93 74 190
Cuts at: 2976 3038 3768 313
Size: 62 730 1539
Fragments arranged by size:
1539 756 730 633 611 190 168 138
93 74 62
BspEI T'CCGG_A
Cuts at: 4214 4214
Size: 4994
BspHI T'CATG_A
Cuts at: 1410 1419 4170 1410
Size: 9 2751 2234
Fragments arranged by size:
2751 2234 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2044 2258 2560 2941 2044
Size: 214 302 381 4097
Fragments arranged by size:
4097 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3286 3340 4824 4989 50
Size: 3236 54 1484 165 55
Fragments arranged by size:
3236 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 558 1677 2906 558
Size: 1119 1229 2646
Fragments arranged by size:
2646 1229 1119
BsrFI r'CCGG_y
Cuts at: 2992 3173 3382 2992
Size: 181 209 4604
Fragments arranged by size:
4604 209 181
BsrGI T'GTAC_A
Cuts at: 1461 1461
Size: 4994
BssSI C'ACGA_G
Cuts at: 3265 3623 3265
Size: 358 4636
BstAPI GCAn_nnn'nTGC
Cuts at: 2371 2371
Size: 4994
BstBI TT'CG_AA
Cuts at: 3355 3355
Size: 4994
BstYI r'GATC_y
Cuts at: 1 817 1363 1405 2019 2486 2509 2844 3090
Size: 816 546 42 614 467 23 335 246
Cuts at: 3090 4102 4188 4200 4546 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 816 614 546 467 449 346 335
246 86 42 23 12
BstZ17I GTA'TAC
Cuts at: 461 461
Size: 4994
Bsu36I CC'TnA_GG
Cuts at: 347 347
Size: 4994
BtgI C'CryG_G
Cuts at: 107 444 107
Size: 337 4657
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2944 3125 3288 2944
Size: 181 163 4650
Fragments arranged by size:
4650 181 163
BtsI GCAGTG_nn'
Cuts at: 716 837 1089 1173 1257 1509 1761 1845 2237
Size: 121 252 84 84 252 252 84 392
Cuts at: 2237 716
Size: 3473
Fragments arranged by size:
3473 392 252 252 252 121 84 84
84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1659 1694 1659
Size: 35 4959
DraI TTT'AAA
Cuts at: 1993 1993
Size: 4994
DraIII CAC_nnn'GTG
Cuts at: 231 231
Size: 4994
DrdI GACnn_nn'nnGTC
Cuts at: 2700 3558 2700
Size: 858 4136
EaeI y'GGCC_r
Cuts at: 116 2579 2753 3144 3171 4516 116
Size: 2463 174 391 27 1345 594
Fragments arranged by size:
2463 1345 594 391 174 27
EagI C'GGCC_G
Cuts at: 116 2579 116
Size: 2463 2531
EarI CTCTTCn'nnn_
Cuts at: 1800 3017 3227 1800
Size: 1217 210 3567
Fragments arranged by size:
3567 1217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3202 3512 3658 4804 3202
Size: 310 146 1146 3392
Fragments arranged by size:
3392 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 1700 1868 2006 944
Size: 756 168 138 3932
Fragments arranged by size:
3932 756 168 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 296 528 643 878 998 1082 1418 1586
Size: 37 232 115 235 120 84 336 168
Cuts at: 1586 1670 1686 2819 3251 3998 4266 259
Size: 84 16 1133 432 747 268 987
Fragments arranged by size:
1133 987 747 432 336 268 235 232
168 120 115 84 84 37 16
EcoO109I rG'GnC_Cy
Cuts at: 351 1321 2133 351
Size: 970 812 3212
Fragments arranged by size:
3212 970 812
EcoRI G'AATT_C
Cuts at: 968 1136 1892 1998 968
Size: 168 756 106 3964
Fragments arranged by size:
3964 756 168 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 4994
FspI TGC'GCA
Cuts at: 2775 2775
Size: 4994
HaeII r_GCGC'y
Cuts at: 2428 2676 3698 4677 2428
Size: 248 1022 979 2745
Fragments arranged by size:
2745 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2836 2866 2868 2898 2836
Size: 30 2 30 4932
Fragments arranged by size:
4932 30 30 2
HincII GTy'rAC
Cuts at: 2033 2033
Size: 4994
HindIII A'AGCT_T
Cuts at: 761 761
Size: 4994
KasI G'GCGC_C
Cuts at: 2424 2672 2424
Size: 248 4746
KpnI G_GTAC'C
Cuts at: 2014 2014
Size: 4994
MluI A'CGCG_T
Cuts at: 4427 4427
Size: 4994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 510 1401 1744 1979 3665 3849 510
Size: 891 343 235 1686 184 1655
Fragments arranged by size:
1686 1655 891 343 235 184
MscI TGG'CCA
Cuts at: 2755 2755
Size: 4994
MslI CAynn'nnrTG
Cuts at: 782 995 1424 1497 2983 3110 782
Size: 213 429 73 1486 127 2666
Fragments arranged by size:
2666 1486 429 213 127 73
MspA1I CmG'CkG
Cuts at: 324 581 2105 2419 2779 3792 4037 324
Size: 257 1524 314 360 1013 245 1281
Fragments arranged by size:
1524 1281 1013 360 314 257 245
NaeI GCC'GGC
Cuts at: 3175 3175
Size: 4994
NarI GG'CG_CC
Cuts at: 2425 2673 2425
Size: 248 4746
NcoI C'CATG_G
Cuts at: 107 444 107
Size: 337 4657
NdeI CA'TA_TG
Cuts at: 1494 1494
Size: 4994
NgoMIV G'CCGG_C
Cuts at: 3173 3173
Size: 4994
NspI r_CATG'y
Cuts at: 476 529 570 781 828 914 2047 2547 3454
Size: 53 41 211 47 86 1133 500 907
Cuts at: 3454 476
Size: 2016
Fragments arranged by size:
2016 1133 907 500 211 86 53 47
41
PasI CC'CwG_GG
Cuts at: 138 264 138
Size: 126 4868
PciI A'CATG_T
Cuts at: 566 777 824 910 3450 566
Size: 211 47 86 2540 2110
Fragments arranged by size:
2540 2110 211 86 47
PfoI T'CCnGG_A
Cuts at: 178 178
Size: 4994
PmeI GTTT'AAAC
Cuts at: 1993 1993
Size: 4994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 4133
Size: 32 4962
PstI C_TGCA'G
Cuts at: 2041 2726 2041
Size: 685 4309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4994
PvuII CAG'CTG
Cuts at: 581 2419 2779 581
Size: 1838 360 2796
Fragments arranged by size:
2796 1838 360
RsrII CG'GwC_CG
Cuts at: 3189 3189
Size: 4994
SacI G_AGCT'C
Cuts at: 946 1702 1870 2008 946
Size: 756 168 138 3932
Fragments arranged by size:
3932 756 168 138
SalI G'TCGA_C
Cuts at: 2031 2031
Size: 4994
SapI GCTCTTCn'nnn_
Cuts at: 3017 3227 3017
Size: 210 4784
SbfI CC_TGCA'GG
Cuts at: 2041 2041
Size: 4994
ScaI AGT'ACT
Cuts at: 4511 4511
Size: 4994
SfcI C'TryA_G
Cuts at: 33 861 933 2037 2722 3715 3906 33
Size: 828 72 1104 685 993 191 1121
Fragments arranged by size:
1121 1104 993 828 685 191 72
SfoI GGC'GCC
Cuts at: 2426 2674 2426
Size: 248 4746
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
SmaI CCC'GGG
Cuts at: 2016 2016
Size: 4994
SmlI C'TyrA_G
Cuts at: 402 2149 3064 3556 3818 402
Size: 1747 915 492 262 1578
Fragments arranged by size:
1747 1578 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4994
SphI G_CATG'C
Cuts at: 2047 2547 2047
Size: 500 4494
StuI AGG'CCT
Cuts at: 1071 1155 1239 1575 1659 1743 1827 1911 1071
Size: 84 84 336 84 84 84 84 4154
Fragments arranged by size:
4154 336 84 84 84 84 84 84
StyI C'CwwG_G
Cuts at: 107 432 444 2128 2482 107
Size: 325 12 1684 354 2619
Fragments arranged by size:
2619 1684 354 325 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 2577 2710 3169 3378 256
Size: 2321 133 459 209 1872
Fragments arranged by size:
2321 1872 459 209 133
TatI w'GTAC_w
Cuts at: 1461 4509 1461
Size: 3048 1946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 740 772 4511 4543 740
Size: 32 3739 32 1191
Fragments arranged by size:
3739 1191 32 32
Tth111I GACn'n_nGTC
Cuts at: 2791 2791
Size: 4994
XbaI T'CTAG_A
Cuts at: 2025 2025
Size: 4994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 2489 708
Size: 1781 3213
XmaI C'CCGG_G
Cuts at: 2014 2014
Size: 4994
XmnI GAAnn'nnTTC
Cuts at: 541 607 634 763 541
Size: 66 27 129 4772
Fragments arranged by size:
4772 129 66 27
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstXI ClaI EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI