Restriction map for
pF1KB7855
(Circular) (Six-base) MAPSORT of: pF1KB7855.seq Check: 1637 from: 1 to: 4766
pF1KB7855 4766 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1386 1386
Size: 4766
AccI GT'mk_AC
Cuts at: 1514 1804 1514
Size: 290 4476
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 4606 4606
Size: 4766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3023 3770 4038 2591
Size: 432 747 268 3319
Fragments arranged by size:
3319 747 432 268
AflIII A'CryG_T
Cuts at: 2118 3222 4199 2118
Size: 1104 977 2685
Fragments arranged by size:
2685 1104 977
AgeI A'CCGG_T
Cuts at: 3154 3154
Size: 4766
AleI CACnn'nnGTG
Cuts at: 1204 1204
Size: 4766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1120 1120
Size: 4766
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 4734
AlwNI CAG_nnn'CTG
Cuts at: 453 972 1126 1285 1333 1536 1539 1651 2071
Size: 519 154 159 48 203 3 112 420
Cuts at: 2071 3638 453
Size: 1567 1581
Fragments arranged by size:
1581 1567 519 420 203 159 154 112
48 3
ApaI G_GGCC'C
Cuts at: 1629 1668 1629
Size: 39 4727
ApaLI G'TGCA_C
Cuts at: 838 3536 838
Size: 2698 2068
ApoI r'AATT_y
Cuts at: 961 1770 4119 4375 961
Size: 809 2349 256 1352
Fragments arranged by size:
2349 1352 809 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 812 1668 1786 812
Size: 856 118 3792
Fragments arranged by size:
3792 856 118
BamHI G'GATC_C
Cuts at: 1791 1791
Size: 4766
BanI G'GyrC_C
Cuts at: 1782 2196 2444 2479 1782
Size: 414 248 35 4069
Fragments arranged by size:
4069 414 248 35
BanII G_rGCy'C
Cuts at: 419 689 947 956 1580 1629 1668 1676 1780
Size: 270 258 9 624 49 39 8 104
Cuts at: 1780 2810 419
Size: 1030 2375
Fragments arranged by size:
2375 1030 624 270 258 104 49 39
9 8
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BbsI GAAGACnn'nnnn_
Cuts at: 1457 1709 1457
Size: 252 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 553 587 708 742 553
Size: 34 121 34 4577
Fragments arranged by size:
4577 121 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3425 4766 2664
Size: 761 1341 2664
Fragments arranged by size:
2664 1341 761
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BglI GCCn_nnn'nGGC
Cuts at: 398 398
Size: 4766
BglII A'GATC_T
Cuts at: 692 740 2281 4318 692
Size: 48 1541 2037 1140
Fragments arranged by size:
2037 1541 1140 48
BlpI GC'TnA_GC
Cuts at: 413 1119 1878 413
Size: 706 759 3301
Fragments arranged by size:
3301 759 706
Bme1580I G_kGCm'C
Cuts at: 672 842 1629 1668 2391 2484 3540 672
Size: 170 787 39 723 93 1056 1898
Fragments arranged by size:
1898 1056 787 723 170 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 1333 1669 1978 2059 2394 4538 1333
Size: 336 309 81 335 2144 1561
Fragments arranged by size:
2144 1561 336 335 309 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 3133 246
Size: 2887 1879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1262 1280 505
Size: 757 18 3991
Fragments arranged by size:
3991 757 18
Bpu10I CC'TnA_GC
Cuts at: 1171 1171
Size: 4766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1942 2821 3313 3611 673
Size: 1269 879 492 298 1828
Fragments arranged by size:
1828 1269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1021 4470 1021
Size: 3449 1317
BsaAI yAC'GTr
Cuts at: 2119 2119
Size: 4766
BsaBI GATnn'nnATC
Cuts at: 6 2280 4317 6
Size: 2274 2037 455
Fragments arranged by size:
2274 2037 455
BsaHI Gr'CG_yC
Cuts at: 2197 2445 3147 4643 2197
Size: 248 702 1496 2320
Fragments arranged by size:
2320 1496 702 248
BsaWI w'CCGG_w
Cuts at: 863 995 1955 2476 3154 3428 3575 3986 863
Size: 132 960 521 678 274 147 411 1643
Fragments arranged by size:
1643 960 678 521 411 274 147 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1696 1726 1696
Size: 30 4736
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 270 341 1018 1349 1701 270
Size: 71 677 331 352 3335
Fragments arranged by size:
3335 677 352 331 71
BseYI C'CCAG_C
Cuts at: 803 886 1145 1326 1399 1485 1644 2191 2949
Size: 83 259 181 73 86 159 547 758
Cuts at: 2949 3526 4022 803
Size: 577 496 1547
Fragments arranged by size:
1547 758 577 547 496 259 181 159
86 83 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 784
Size: 4766
BsiEI CG_ry'CG
Cuts at: 104 939 990 2308 2354 3562 104
Size: 835 51 1318 46 1208 1308
Fragments arranged by size:
1318 1308 1208 835 51 46
BsiHKAI G_wGCw'C
Cuts at: 262 419 689 842 947 1177 1780 2558 2748
Size: 157 270 153 105 230 603 778 190
Cuts at: 2748 3540 262
Size: 792 1488
Fragments arranged by size:
1488 792 778 603 270 230 190 157
153 105
BsmI GAATG_Cn'
Cuts at: 576 576
Size: 4766
Bsp1286I G_dGCh'C
Cuts at: 262 419 672 689 842 947 956 1177 1580
Size: 157 253 17 153 105 9 221 403
Cuts at: 1580 1629 1668 1676 1780 2391 2484 2558 2748
Size: 49 39 8 104 611 93 74 190
Cuts at: 2748 2810 3540 262
Size: 62 730 1488
Fragments arranged by size:
1488 730 611 403 253 221 190 157
153 105 104 93 74 62 49 39
17 9 8
BspEI T'CCGG_A
Cuts at: 863 3986 863
Size: 3123 1643
BspHI T'CATG_A
Cuts at: 1043 3942 1043
Size: 2899 1867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 980 1386 1816 2030 2332 2713 980
Size: 406 430 214 302 381 3033
Fragments arranged by size:
3033 430 406 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3058 3112 4596 4761 50
Size: 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1097 1715 2678 1097
Size: 618 963 3185
Fragments arranged by size:
3185 963 618
BsrFI r'CCGG_y
Cuts at: 356 2764 2945 3154 356
Size: 2408 181 209 1968
Fragments arranged by size:
2408 1968 209 181
BssSI C'ACGA_G
Cuts at: 370 3037 3395 370
Size: 2667 358 1741
Fragments arranged by size:
2667 1741 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2143 2143
Size: 4766
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstEII G'GTnAC_C
Cuts at: 336 336
Size: 4766
BstXI CCAn_nnnn'nTGG
Cuts at: 157 1587 157
Size: 1430 3336
BstYI r'GATC_y
Cuts at: 1 692 740 1791 2258 2281 2616 2862 3874
Size: 691 48 1051 467 23 335 246 1012
Cuts at: 3874 3960 3972 4318 1
Size: 86 12 346 449
Fragments arranged by size:
1051 1012 691 467 449 346 335 246
86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 1361 1598 1361
Size: 237 4529
BtgI C'CryG_G
Cuts at: 107 233 956 1202 1301 107
Size: 126 723 246 99 3572
Fragments arranged by size:
3572 723 246 126 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 677 2009 677
Size: 1332 3434
DraI TTT'AAA
Cuts at: 1765 1765
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3330 2472
Size: 858 3908
EaeI y'GGCC_r
Cuts at: 851 2351 2525 2916 2943 4288 851
Size: 1500 174 391 27 1345 1329
Fragments arranged by size:
1500 1345 1329 391 174 27
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 539 1045 2789 2999 539
Size: 506 1744 210 2306
Fragments arranged by size:
2306 1744 506 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 616 2974 3284 3430 4576 616
Size: 2358 310 146 1146 806
Fragments arranged by size:
2358 1146 806 310 146
EcoICRI GAG'CTC
Cuts at: 417 687 945 1778 417
Size: 270 258 833 3405
Fragments arranged by size:
3405 833 270 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1262 1280 2591 3023 3770 4038 505
Size: 757 18 1311 432 747 268 1233
Fragments arranged by size:
1311 1233 757 747 432 268 18
EcoNI CCTnn'n_nnAGG
Cuts at: 487 1270 487
Size: 783 3983
EcoO109I rG'GnC_Cy
Cuts at: 345 480 606 620 1323 1354 1422 1460 1625
Size: 135 126 14 703 31 68 38 165
Cuts at: 1625 1634 1664 1665 1905 345
Size: 9 30 1 240 3206
Fragments arranged by size:
3206 703 240 165 135 126 68 38
31 30 14 9 1
EcoRI G'AATT_C
Cuts at: 961 1770 961
Size: 809 3957
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4766
FspI TGC'GCA
Cuts at: 325 2547 325
Size: 2222 2544
FspAI rTGC'GCAy
Cuts at: 325 325
Size: 4766
HaeII r_GCGC'y
Cuts at: 231 2200 2448 3470 4449 231
Size: 1969 248 1022 979 548
Fragments arranged by size:
1969 1022 979 548 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1524 1696 1728 2608 2638 2640 2670 1492
Size: 32 172 32 880 30 2 30 3588
Fragments arranged by size:
3588 880 172 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1515 1805 1515
Size: 290 4476
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 959 3437 3621 959
Size: 2478 184 2104
Fragments arranged by size:
2478 2104 184
MscI TGG'CCA
Cuts at: 853 2527 853
Size: 1674 3092
MslI CAynn'nnrTG
Cuts at: 781 1102 1204 1555 1585 2755 2882 781
Size: 321 102 351 30 1170 127 2665
Fragments arranged by size:
2665 1170 351 321 127 102 30
MspA1I CmG'CkG
Cuts at: 195 235 431 722 921 1064 1434 1489 1536
Size: 40 196 291 199 143 370 55 47
Cuts at: 1536 1877 2191 2551 3564 3809 195
Size: 341 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 370 360 341 314 291 245
199 196 143 55 47 40
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NcoI C'CATG_G
Cuts at: 107 956 1202 1301 107
Size: 849 246 99 3572
Fragments arranged by size:
3572 849 246 99
NdeI CA'TA_TG
Cuts at: 1023 1023
Size: 4766
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NspI r_CATG'y
Cuts at: 204 1819 2319 3226 204
Size: 1615 500 907 1744
Fragments arranged by size:
1744 1615 907 500
PciI A'CATG_T
Cuts at: 3222 3222
Size: 4766
PflMI CCAn_nnn'nTGG
Cuts at: 453 453
Size: 4766
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 697 729 3905 3937 697
Size: 32 3176 32 1526
Fragments arranged by size:
3176 1526 32 32
PpuMI rG'GwC_Cy
Cuts at: 345 480 606 345
Size: 135 126 4505
Fragments arranged by size:
4505 135 126
PshAI GACnn'nnGTC
Cuts at: 989 989
Size: 4766
PspOMI G'GGCC_C
Cuts at: 1625 1664 1625
Size: 39 4727
PstI C_TGCA'G
Cuts at: 896 1433 1439 1813 2498 896
Size: 537 6 374 685 3164
Fragments arranged by size:
3164 685 537 374 6
PvuI CG_AT'CG
Cuts at: 104 990 104
Size: 886 3880
PvuII CAG'CTG
Cuts at: 722 921 1434 1489 1536 2191 2551 722
Size: 199 513 55 47 655 360 2937
Fragments arranged by size:
2937 655 513 360 199 55 47
RsrII CG'GwC_CG
Cuts at: 426 2961 426
Size: 2535 2231
SacI G_AGCT'C
Cuts at: 419 689 947 1780 419
Size: 270 258 833 3405
Fragments arranged by size:
3405 833 270 258
SacII CC_GC'GG
Cuts at: 236 236
Size: 4766
SalI G'TCGA_C
Cuts at: 1513 1803 1513
Size: 290 4476
SanDI GG'GwC_CC
Cuts at: 606 606
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 539 2789 2999 539
Size: 2250 210 2306
Fragments arranged by size:
2306 2250 210
SbfI CC_TGCA'GG
Cuts at: 896 1813 896
Size: 917 3849
ScaI AGT'ACT
Cuts at: 4283 4283
Size: 4766
SfcI C'TryA_G
Cuts at: 33 539 892 1429 1435 1809 2494 3487 3678
Size: 506 353 537 6 374 685 993 191
Cuts at: 3678 33
Size: 1121
Fragments arranged by size:
1121 993 685 537 506 374 353 191
6
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1670 1788 1670
Size: 118 4648
SmlI C'TyrA_G
Cuts at: 688 1921 2836 3328 3590 688
Size: 1233 915 492 262 1864
Fragments arranged by size:
1864 1233 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 204 1819 2319 204
Size: 1615 500 2651
Fragments arranged by size:
2651 1615 500
StuI AGG'CCT
Cuts at: 154 1234 154
Size: 1080 3686
StyI C'CwwG_G
Cuts at: 107 341 956 1202 1241 1301 1900 2254 107
Size: 234 615 246 39 60 599 354 2619
Fragments arranged by size:
2619 615 599 354 246 234 60 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1408 2349 2482 2941 3150 1408
Size: 941 133 459 209 3024
Fragments arranged by size:
3024 941 459 209 133
TatI w'GTAC_w
Cuts at: 4281 4281
Size: 4766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 4734
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1722 2261 1722
Size: 539 4227
XmaI C'CCGG_G
Cuts at: 1668 1786 1668
Size: 118 4648
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI