Restriction map for
pF1KB7852
(Circular) (Six-base) MAPSORT of: pF1KB7852.seq Check: 9724 from: 1 to: 4955
pF1KB7852 4955 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1566 1566
Size: 4955
AccI GT'mk_AC
Cuts at: 1993 1993
Size: 4955
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 224 1237 1530 1885 1934 2780 3212 3959
Size: 15 1013 293 355 49 846 432 747
Cuts at: 3959 4227 209
Size: 268 937
Fragments arranged by size:
1013 937 846 747 432 355 293 268
49 15
AflIII A'CryG_T
Cuts at: 2307 3411 4388 2307
Size: 1104 977 2874
Fragments arranged by size:
2874 1104 977
AgeI A'CCGG_T
Cuts at: 3343 3343
Size: 4955
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 542 574 542
Size: 32 4923
AlwNI CAG_nnn'CTG
Cuts at: 247 307 958 2260 3827 247
Size: 60 651 1302 1567 1375
Fragments arranged by size:
1567 1375 1302 651 60
ApaI G_GGCC'C
Cuts at: 1858 1858
Size: 4955
ApaLI G'TGCA_C
Cuts at: 3725 3725
Size: 4955
ApoI r'AATT_y
Cuts at: 583 679 909 1389 1959 4308 4564 583
Size: 96 230 480 570 2349 256 974
Fragments arranged by size:
2349 974 570 480 256 230 96
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 1252 1975 1252
Size: 723 4232
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 1256 1971 2385 2633 2668 1256
Size: 715 414 248 35 3543
Fragments arranged by size:
3543 715 414 248 35
BanII G_rGCy'C
Cuts at: 1858 1969 2999 1858
Size: 111 1030 3814
Fragments arranged by size:
3814 1030 111
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BciVI GTATCCnnnnn_n'
Cuts at: 2853 3614 4955 2853
Size: 761 1341 2853
Fragments arranged by size:
2853 1341 761
BclI T'GATC_A
Cuts at: 2475 2475
Size: 4955
BglII A'GATC_T
Cuts at: 2470 4507 2470
Size: 2037 2918
BlpI GC'TnA_GC
Cuts at: 2067 2067
Size: 4955
Bme1580I G_kGCm'C
Cuts at: 1858 2580 2673 3729 1858
Size: 722 93 1056 3084
Fragments arranged by size:
3084 1056 722 93
BmgBI CAC'GTC
Cuts at: 234 234
Size: 4955
BmrI ACTGGGnnnn_n'
Cuts at: 801 1134 1861 2167 2248 2583 4727 801
Size: 333 727 306 81 335 2144 1029
Fragments arranged by size:
2144 1029 727 335 333 306 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1001 3322 1001
Size: 2321 2634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 721
Size: 4955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2131 3010 3502 3800 2131
Size: 879 492 298 3286
Fragments arranged by size:
3286 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4659 4659
Size: 4955
BsaAI yAC'GTr
Cuts at: 2308 2308
Size: 4955
BsaBI GATnn'nnATC
Cuts at: 6 1843 2469 4506 6
Size: 1837 626 2037 455
Fragments arranged by size:
2037 1837 626 455
BsaHI Gr'CG_yC
Cuts at: 2386 2634 3336 4832 2386
Size: 248 702 1496 2509
Fragments arranged by size:
2509 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2144 2665 3343 3617 3764 4175 2144
Size: 521 678 274 147 411 2924
Fragments arranged by size:
2924 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1857 2380 3138 3715 4211 1857
Size: 523 758 577 496 2601
Fragments arranged by size:
2601 758 577 523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 538 233
Size: 305 4650
BsiEI CG_ry'CG
Cuts at: 104 1160 1365 2497 2543 3751 104
Size: 1056 205 1132 46 1208 1308
Fragments arranged by size:
1308 1208 1132 1056 205 46
BsiHKAI G_wGCw'C
Cuts at: 1969 2747 2937 3729 1969
Size: 778 190 792 3195
Fragments arranged by size:
3195 792 778 190
BsmI GAATG_Cn'
Cuts at: 1896 1896
Size: 4955
Bsp1286I G_dGCh'C
Cuts at: 1858 1969 2580 2673 2747 2937 2999 3729 1858
Size: 111 611 93 74 190 62 730 3084
Fragments arranged by size:
3084 730 611 190 111 93 74 62
BspEI T'CCGG_A
Cuts at: 4175 4175
Size: 4955
BspHI T'CATG_A
Cuts at: 4131 4131
Size: 4955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1566 2005 2219 2521 2902 1566
Size: 439 214 302 381 3619
Fragments arranged by size:
3619 439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3247 3301 4785 4950 50
Size: 3197 54 1484 165 55
Fragments arranged by size:
3197 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2867 2867
Size: 4955
BsrFI r'CCGG_y
Cuts at: 2953 3134 3343 2953
Size: 181 209 4565
Fragments arranged by size:
4565 209 181
BsrGI T'GTAC_A
Cuts at: 1485 1734 1485
Size: 249 4706
BssSI C'ACGA_G
Cuts at: 3226 3584 3226
Size: 358 4597
BstAPI GCAn_nnn'nTGC
Cuts at: 958 1578 2332 958
Size: 620 754 3581
Fragments arranged by size:
3581 754 620
BstBI TT'CG_AA
Cuts at: 3316 3316
Size: 4955
BstEII G'GTnAC_C
Cuts at: 439 439
Size: 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 527 847 851 1279 1698 527
Size: 320 4 428 419 3784
Fragments arranged by size:
3784 428 419 320 4
BstYI r'GATC_y
Cuts at: 1 1220 1980 2447 2470 2805 3051 4063 4149
Size: 1219 760 467 23 335 246 1012 86
Cuts at: 4149 4161 4507 1
Size: 12 346 449
Fragments arranged by size:
1219 1012 760 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 563 1929 563
Size: 1366 3589
BtgI C'CryG_G
Cuts at: 707 1278 1604 707
Size: 571 326 4058
Fragments arranged by size:
4058 571 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 833 2905 3086 3249 833
Size: 2072 181 163 2539
Fragments arranged by size:
2539 2072 181 163
BtsI GCAGTG_nn'
Cuts at: 1653 2198 1653
Size: 545 4410
ClaI AT'CG_AT
Cuts at: 1790 1790
Size: 4955
DraI TTT'AAA
Cuts at: 972 1954 972
Size: 982 3973
DrdI GACnn_nn'nnGTC
Cuts at: 2661 3519 2661
Size: 858 4097
EaeI y'GGCC_r
Cuts at: 2540 2714 3105 3132 4477 2540
Size: 174 391 27 1345 3018
Fragments arranged by size:
3018 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2540 2540
Size: 4955
EarI CTCTTCn'nnn_
Cuts at: 185 1410 1549 1748 1751 2978 3188 185
Size: 1225 139 199 3 1227 210 1952
Fragments arranged by size:
1952 1227 1225 210 199 139 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 3473 3619 4765 3163
Size: 310 146 1146 3353
Fragments arranged by size:
3353 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1967 1967
Size: 4955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 224 721 1237 1530 1885 1934 2780 3212
Size: 15 497 516 293 355 49 846 432
Cuts at: 3212 3959 4227 209
Size: 747 268 937
Fragments arranged by size:
937 846 747 516 497 432 355 293
268 49 15
EcoO109I rG'GnC_Cy
Cuts at: 1629 2094 1629
Size: 465 4490
EcoRI G'AATT_C
Cuts at: 1959 1959
Size: 4955
EcoRV GAT'ATC
Cuts at: 1149 1149
Size: 4955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4955
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 2389 2637 3659 4638 2389
Size: 248 1022 979 2706
Fragments arranged by size:
2706 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1912 1944 2797 2827 2829 2859 1912
Size: 32 853 30 2 30 4008
Fragments arranged by size:
4008 853 32 30 30 2
HincII GTy'rAC
Cuts at: 1994 1994
Size: 4955
HindIII A'AGCT_T
Cuts at: 133 133
Size: 4955
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 669 1687 3626 3810 669
Size: 1018 1939 184 1814
Fragments arranged by size:
1939 1814 1018 184
MscI TGG'CCA
Cuts at: 2716 2716
Size: 4955
MslI CAynn'nnrTG
Cuts at: 350 461 1097 1115 1277 1304 2944 3071 350
Size: 111 636 18 162 27 1640 127 2234
Fragments arranged by size:
2234 1640 636 162 127 111 27 18
MspA1I CmG'CkG
Cuts at: 244 2066 2380 2740 3753 3998 244
Size: 1822 314 360 1013 245 1201
Fragments arranged by size:
1822 1201 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 4955
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 4955
NspI r_CATG'y
Cuts at: 1765 2008 2508 3415 1765
Size: 243 500 907 3305
Fragments arranged by size:
3305 907 500 243
PciI A'CATG_T
Cuts at: 3411 3411
Size: 4955
PfoI T'CCnGG_A
Cuts at: 916 916
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1073 1105 4094 4126 1073
Size: 32 2989 32 1902
Fragments arranged by size:
2989 1902 32 32
PpuMI rG'GwC_Cy
Cuts at: 1629 1629
Size: 4955
PspOMI G'GGCC_C
Cuts at: 1854 1854
Size: 4955
PstI C_TGCA'G
Cuts at: 2002 2687 2002
Size: 685 4270
PvuI CG_AT'CG
Cuts at: 104 1160 1365 104
Size: 1056 205 3694
Fragments arranged by size:
3694 1056 205
PvuII CAG'CTG
Cuts at: 2380 2740 2380
Size: 360 4595
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1969 1969
Size: 4955
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 1751 2978 3188 1751
Size: 1227 210 3518
Fragments arranged by size:
3518 1227 210
SbfI CC_TGCA'GG
Cuts at: 2002 2002
Size: 4955
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SfcI C'TryA_G
Cuts at: 33 422 1431 1998 2683 3676 3867 33
Size: 389 1009 567 685 993 191 1121
Fragments arranged by size:
1121 1009 993 685 567 389 191
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 1254 1977 1254
Size: 723 4232
SmlI C'TyrA_G
Cuts at: 2110 3025 3517 3779 2110
Size: 915 492 262 3286
Fragments arranged by size:
3286 915 492 262
SpeI A'CTAG_T
Cuts at: 67 774 67
Size: 707 4248
SphI G_CATG'C
Cuts at: 1765 2008 2508 1765
Size: 243 500 4212
Fragments arranged by size:
4212 500 243
StuI AGG'CCT
Cuts at: 610 610
Size: 4955
StyI C'CwwG_G
Cuts at: 206 323 728 888 2089 2443 206
Size: 117 405 160 1201 354 2718
Fragments arranged by size:
2718 1201 405 354 160 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1116 2538 2671 3130 3339 1116
Size: 1422 133 459 209 2732
Fragments arranged by size:
2732 1422 459 209 133
TatI w'GTAC_w
Cuts at: 1485 1734 4470 1485
Size: 249 2736 1970
Fragments arranged by size:
2736 1970 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1167 1199 4472 4504 1167
Size: 32 3273 32 1618
Fragments arranged by size:
3273 1618 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 2752 1828
Size: 924 4031
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 2450 156
Size: 2294 2661
XmaI C'CCGG_G
Cuts at: 1252 1975 1252
Size: 723 4232
XmnI GAAnn'nnTTC
Cuts at: 640 640
Size: 4955
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BssHII BstZ17I CspCI DraIII
EcoNI FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI