Restriction map for
pF1KB7850
(Circular) (Six-base) MAPSORT of: pF1KB7850.seq Check: 1573 from: 1 to: 4982
pF1KB7850 4982 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 227 2020 227
Size: 1793 3189
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 4822 4822
Size: 4982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 137 847 1543 1724 1736 1946 2807 3239
Size: 16 710 696 181 12 210 861 432
Cuts at: 3239 3986 4254 121
Size: 747 268 849
Fragments arranged by size:
861 849 747 710 696 432 268 210
181 16 12
AflIII A'CryG_T
Cuts at: 873 1483 1485 1722 2334 3438 4415 873
Size: 610 2 237 612 1104 977 1440
Fragments arranged by size:
1440 1104 977 612 610 237 2
AgeI A'CCGG_T
Cuts at: 200 3370 200
Size: 3170 1812
AleI CACnn'nnGTG
Cuts at: 1646 1646
Size: 4982
AlwNI CAG_nnn'CTG
Cuts at: 601 1858 2287 3854 601
Size: 1257 429 1567 1729
Fragments arranged by size:
1729 1567 1257 429
ApaLI G'TGCA_C
Cuts at: 1630 3752 1630
Size: 2122 2860
ApoI r'AATT_y
Cuts at: 289 1085 1694 1986 4335 4591 289
Size: 796 609 292 2349 256 680
Fragments arranged by size:
2349 796 680 609 292 256
AseI AT'TA_AT
Cuts at: 20 388 20
Size: 368 4614
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 2002 2002
Size: 4982
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1703 1736 1703
Size: 33 4949
BamHI G'GATC_C
Cuts at: 2007 2007
Size: 4982
BanI G'GyrC_C
Cuts at: 631 1998 2412 2660 2695 631
Size: 1367 414 248 35 2918
Fragments arranged by size:
2918 1367 414 248 35
BanII G_rGCy'C
Cuts at: 1996 3026 1996
Size: 1030 3952
BbeI G_GCGC'C
Cuts at: 2416 2664 2416
Size: 248 4734
BbvCI CC'TCA_GC
Cuts at: 1862 1892 1862
Size: 30 4952
BciVI GTATCCnnnnn_n'
Cuts at: 2880 3641 4982 2880
Size: 761 1341 2880
Fragments arranged by size:
2880 1341 761
BclI T'GATC_A
Cuts at: 2502 2502
Size: 4982
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4982
BglII A'GATC_T
Cuts at: 1608 2497 4534 1608
Size: 889 2037 2056
Fragments arranged by size:
2056 2037 889
BlpI GC'TnA_GC
Cuts at: 2094 2094
Size: 4982
Bme1580I G_kGCm'C
Cuts at: 1634 2607 2700 3756 1634
Size: 973 93 1056 2860
Fragments arranged by size:
2860 1056 973 93
BmrI ACTGGGnnnn_n'
Cuts at: 1085 2194 2275 2610 4754 1085
Size: 1109 81 335 2144 1313
Fragments arranged by size:
2144 1313 1109 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 4982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1373 1600 1268
Size: 105 227 4650
Fragments arranged by size:
4650 227 105
Bpu10I CC'TnA_GC
Cuts at: 1862 1892 1862
Size: 30 4952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2158 3037 3529 3827 2158
Size: 879 492 298 3313
Fragments arranged by size:
3313 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1897 4686 1897
Size: 2789 2193
BsaAI yAC'GTr
Cuts at: 1486 1806 2335 1486
Size: 320 529 4133
Fragments arranged by size:
4133 529 320
BsaBI GATnn'nnATC
Cuts at: 6 2496 4533 6
Size: 2490 2037 455
Fragments arranged by size:
2490 2037 455
BsaHI Gr'CG_yC
Cuts at: 2413 2661 3363 4859 2413
Size: 248 702 1496 2536
Fragments arranged by size:
2536 1496 702 248
BsaWI w'CCGG_w
Cuts at: 200 2171 2692 3370 3644 3791 4202 200
Size: 1971 521 678 274 147 411 980
Fragments arranged by size:
1971 980 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1825 1855 1825
Size: 30 4952
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1744 1846 1744
Size: 102 4880
BseYI C'CCAG_C
Cuts at: 1316 2407 3165 3742 4238 1316
Size: 1091 758 577 496 2060
Fragments arranged by size:
2060 1091 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 174 2524 2570 3778 104
Size: 70 2350 46 1208 1308
Fragments arranged by size:
2350 1308 1208 70 46
BsiHKAI G_wGCw'C
Cuts at: 715 944 1634 1996 2774 2964 3756 715
Size: 229 690 362 778 190 792 1941
Fragments arranged by size:
1941 792 778 690 362 229 190
BsmI GAATG_Cn'
Cuts at: 136 1291 136
Size: 1155 3827
BsmBI CGTCTCn'nnnn_
Cuts at: 115 115
Size: 4982
Bsp1286I G_dGCh'C
Cuts at: 715 944 1634 1996 2607 2700 2774 2964 3026
Size: 229 690 362 611 93 74 190 62
Cuts at: 3026 3756 715
Size: 730 1941
Fragments arranged by size:
1941 730 690 611 362 229 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4202 4202
Size: 4982
BspHI T'CATG_A
Cuts at: 1191 1820 4158 1191
Size: 629 2338 2015
Fragments arranged by size:
2338 2015 629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1653 2032 2246 2548 2929 1653
Size: 379 214 302 381 3706
Fragments arranged by size:
3706 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 3274 3328 4812 4977 50
Size: 3224 54 1484 165 55
Fragments arranged by size:
3224 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 577 1552 2894 577
Size: 975 1342 2665
Fragments arranged by size:
2665 1342 975
BsrFI r'CCGG_y
Cuts at: 200 2980 3161 3370 200
Size: 2780 181 209 1812
Fragments arranged by size:
2780 1812 209 181
BssSI C'ACGA_G
Cuts at: 825 3253 3611 825
Size: 2428 358 2196
Fragments arranged by size:
2428 2196 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1858 2359 1858
Size: 501 4481
BstBI TT'CG_AA
Cuts at: 1149 3343 1149
Size: 2194 2788
BstXI CCAn_nnnn'nTGG
Cuts at: 642 642
Size: 4982
BstYI r'GATC_y
Cuts at: 1 1608 2007 2474 2497 2832 3078 4090 4176
Size: 1607 399 467 23 335 246 1012 86
Cuts at: 4176 4188 4534 1
Size: 12 346 449
Fragments arranged by size:
1607 1012 467 449 399 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2932 3113 3276 2932
Size: 181 163 4638
Fragments arranged by size:
4638 181 163
BtsI GCAGTG_nn'
Cuts at: 1232 2225 1232
Size: 993 3989
DraI TTT'AAA
Cuts at: 918 1137 1981 918
Size: 219 844 3919
Fragments arranged by size:
3919 844 219
DrdI GACnn_nn'nnGTC
Cuts at: 2688 3546 2688
Size: 858 4124
EaeI y'GGCC_r
Cuts at: 2567 2741 3132 3159 4504 2567
Size: 174 391 27 1345 3045
Fragments arranged by size:
3045 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4982
EarI CTCTTCn'nnn_
Cuts at: 113 3005 3215 113
Size: 2892 210 1880
Fragments arranged by size:
2892 1880 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3190 3500 3646 4792 3190
Size: 310 146 1146 3380
Fragments arranged by size:
3380 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1994 1994
Size: 4982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 137 847 1268 1373 1543 1600 1724 1736
Size: 16 710 421 105 170 57 124 12
Cuts at: 1736 1946 2807 3239 3986 4254 121
Size: 210 861 432 747 268 849
Fragments arranged by size:
861 849 747 710 432 421 268 210
170 124 105 57 16 12
EcoO109I rG'GnC_Cy
Cuts at: 2121 2121
Size: 4982
EcoRI G'AATT_C
Cuts at: 1694 1986 1694
Size: 292 4690
EcoRV GAT'ATC
Cuts at: 997 997
Size: 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1162 1486 3001 1162
Size: 324 1515 3143
Fragments arranged by size:
3143 1515 324
FspI TGC'GCA
Cuts at: 2763 2763
Size: 4982
HaeII r_GCGC'y
Cuts at: 2416 2664 3686 4665 2416
Size: 248 1022 979 2733
Fragments arranged by size:
2733 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1825 1857 2824 2854 2856 2886 1825
Size: 32 967 30 2 30 3921
Fragments arranged by size:
3921 967 32 30 30 2
HincII GTy'rAC
Cuts at: 228 2021 228
Size: 1793 3189
KasI G'GCGC_C
Cuts at: 2412 2660 2412
Size: 248 4734
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MluI A'CGCG_T
Cuts at: 4415 4415
Size: 4982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3653 3837 3653
Size: 184 4798
MscI TGG'CCA
Cuts at: 2743 2743
Size: 4982
MslI CAynn'nnrTG
Cuts at: 640 1160 1424 1646 1886 2971 3098 640
Size: 520 264 222 240 1085 127 2524
Fragments arranged by size:
2524 1085 520 264 240 222 127
MspA1I CmG'CkG
Cuts at: 867 1316 2093 2407 2767 3780 4025 867
Size: 449 777 314 360 1013 245 1824
Fragments arranged by size:
1824 1013 777 449 360 314 245
NaeI GCC'GGC
Cuts at: 3163 3163
Size: 4982
NarI GG'CG_CC
Cuts at: 2413 2661 2413
Size: 248 4734
NcoI C'CATG_G
Cuts at: 107 107
Size: 4982
NgoMIV G'CCGG_C
Cuts at: 3161 3161
Size: 4982
NspI r_CATG'y
Cuts at: 877 1241 1546 1726 1741 2035 2535 3442 877
Size: 364 305 180 15 294 500 907 2417
Fragments arranged by size:
2417 907 500 364 305 294 180 15
PciI A'CATG_T
Cuts at: 873 1722 3438 873
Size: 849 1716 2417
Fragments arranged by size:
2417 1716 849
PfoI T'CCnGG_A
Cuts at: 1616 1616
Size: 4982
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PmlI CAC'GTG
Cuts at: 1486 1806 1486
Size: 320 4662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 702 734 4121 4153 702
Size: 32 3387 32 1531
Fragments arranged by size:
3387 1531 32 32
PstI C_TGCA'G
Cuts at: 591 2029 2714 591
Size: 1438 685 2859
Fragments arranged by size:
2859 1438 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 867 1316 2407 2767 867
Size: 449 1091 360 3082
Fragments arranged by size:
3082 1091 449 360
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 1996 1996
Size: 4982
SalI G'TCGA_C
Cuts at: 226 2019 226
Size: 1793 3189
SapI GCTCTTCn'nnn_
Cuts at: 3005 3215 3005
Size: 210 4772
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4982
ScaI AGT'ACT
Cuts at: 1102 4499 1102
Size: 3397 1585
SfcI C'TryA_G
Cuts at: 33 587 2025 2710 3703 3894 33
Size: 554 1438 685 993 191 1121
Fragments arranged by size:
1438 1121 993 685 554 191
SfoI GGC'GCC
Cuts at: 2414 2662 2414
Size: 248 4734
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 2004 2004
Size: 4982
SmlI C'TyrA_G
Cuts at: 2137 3052 3544 3806 2137
Size: 915 492 262 3313
Fragments arranged by size:
3313 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4982
SphI G_CATG'C
Cuts at: 1241 2035 2535 1241
Size: 794 500 3688
Fragments arranged by size:
3688 794 500
SspI AAT'ATT
Cuts at: 1440 1440
Size: 4982
StyI C'CwwG_G
Cuts at: 107 2116 2470 107
Size: 2009 354 2619
Fragments arranged by size:
2619 2009 354
SwaI ATTT'AAAT
Cuts at: 918 918
Size: 4982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 2565 2698 3157 3366 188
Size: 2377 133 459 209 1804
Fragments arranged by size:
2377 1804 459 209 133
TatI w'GTAC_w
Cuts at: 701 1100 1372 1719 1770 4497 701
Size: 399 272 347 51 2727 1186
Fragments arranged by size:
2727 1186 399 347 272 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 4950
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4982
XbaI T'CTAG_A
Cuts at: 1680 1785 2013 1680
Size: 105 228 4649
Fragments arranged by size:
4649 228 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1914 2477 1914
Size: 563 4419
XmaI C'CCGG_G
Cuts at: 2002 2002
Size: 4982
XmnI GAAnn'nnTTC
Cuts at: 134 1145 1466 134
Size: 1011 321 3650
Fragments arranged by size:
3650 1011 321
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BcgI BfrBI BmgBI BmtI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI XhoI ZraI