Restriction map for
pF1KB7849
(Circular) (Six-base) MAPSORT of: pF1KB7849.seq Check: 2554 from: 1 to: 4748
pF1KB7849 4748 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1786 1786
Size: 4748
Acc65I G'GTAC_C
Cuts at: 1193 1764 1193
Size: 571 4177
AclI AA'CG_TT
Cuts at: 4588 4588
Size: 4748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 1173 1425 1509 1677 2573 3005 3752 4020
Size: 252 252 84 168 896 432 747 268
Cuts at: 4020 921
Size: 1649
Fragments arranged by size:
1649 896 747 432 268 252 252 168
84
AflIII A'CryG_T
Cuts at: 795 963 1299 1719 2100 3204 4181 795
Size: 168 336 420 381 1104 977 1362
Fragments arranged by size:
1362 1104 977 420 381 336 168
AgeI A'CCGG_T
Cuts at: 3136 3136
Size: 4748
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 214
Size: 32 4716
AlwNI CAG_nnn'CTG
Cuts at: 703 2053 3620 703
Size: 1350 1567 1831
Fragments arranged by size:
1831 1567 1350
ApaLI G'TGCA_C
Cuts at: 499 537 1633 3518 499
Size: 38 1096 1885 1729
Fragments arranged by size:
1885 1729 1096 38
ApoI r'AATT_y
Cuts at: 1752 4101 4357 1752
Size: 2349 256 2143
Fragments arranged by size:
2349 2143 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4748
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
AvaI C'yCGr_G
Cuts at: 1768 1768
Size: 4748
BamHI G'GATC_C
Cuts at: 1773 1773
Size: 4748
BanI G'GyrC_C
Cuts at: 1193 1764 2178 2426 2461 1193
Size: 571 414 248 35 3480
Fragments arranged by size:
3480 571 414 248 35
BanII G_rGCy'C
Cuts at: 1762 2792 1762
Size: 1030 3718
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4500
BbsI GAAGACnn'nnnn_
Cuts at: 853 1302 853
Size: 449 4299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 540 574 540
Size: 34 4714
BciVI GTATCCnnnnn_n'
Cuts at: 2646 3407 4748 2646
Size: 761 1341 2646
Fragments arranged by size:
2646 1341 761
BclI T'GATC_A
Cuts at: 2268 2268
Size: 4748
BfrBI ATG'CAT
Cuts at: 829 829
Size: 4748
BglII A'GATC_T
Cuts at: 574 2263 4300 574
Size: 1689 2037 1022
Fragments arranged by size:
2037 1689 1022
BlpI GC'TnA_GC
Cuts at: 1860 1860
Size: 4748
Bme1580I G_kGCm'C
Cuts at: 503 541 1637 2373 2466 3522 503
Size: 38 1096 736 93 1056 1729
Fragments arranged by size:
1729 1096 1056 736 93 38
BmrI ACTGGGnnnn_n'
Cuts at: 1960 2041 2376 4520 1960
Size: 81 335 2144 2188
Fragments arranged by size:
2188 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 4748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 404 1001 1085 1421 1673 1757 229
Size: 175 597 84 336 252 84 3220
Fragments arranged by size:
3220 597 336 252 175 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 675 1924 2803 3295 3593 495
Size: 180 1249 879 492 298 1650
Fragments arranged by size:
1650 1249 879 492 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 205 463 585 1653 4452 205
Size: 258 122 1068 2799 501
Fragments arranged by size:
2799 1068 501 258 122
BsaAI yAC'GTr
Cuts at: 714 882 1050 1386 1638 2101 714
Size: 168 168 336 252 463 3361
Fragments arranged by size:
3361 463 336 252 168 168
BsaBI GATnn'nnATC
Cuts at: 6 2262 4299 6
Size: 2256 2037 455
Fragments arranged by size:
2256 2037 455
BsaHI Gr'CG_yC
Cuts at: 2179 2427 3129 4625 2179
Size: 248 702 1496 2302
Fragments arranged by size:
2302 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1231 1937 2458 3136 3410 3557 3968 1231
Size: 706 521 678 274 147 411 2011
Fragments arranged by size:
2011 706 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 423 453 214
Size: 30 179 30 4509
Fragments arranged by size:
4509 179 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 198 1023 1191 1359 1443 198
Size: 825 168 168 84 3503
Fragments arranged by size:
3503 825 168 168 84
BseYI C'CCAG_C
Cuts at: 426 2173 2931 3508 4004 426
Size: 1747 758 577 496 1170
Fragments arranged by size:
1747 1170 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2290 2336 3544 104
Size: 2186 46 1208 1308
Fragments arranged by size:
2186 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 503 541 1215 1637 1762 2540 2730 3522 503
Size: 38 674 422 125 778 190 792 1729
Fragments arranged by size:
1729 792 778 674 422 190 125 38
BsiWI C'GTAC_G
Cuts at: 1496 1496
Size: 4748
BsmI GAATG_Cn'
Cuts at: 1180 1229 1675 1180
Size: 49 446 4253
Fragments arranged by size:
4253 446 49
BsmBI CGTCTCn'nnnn_
Cuts at: 1124 1124
Size: 4748
Bsp1286I G_dGCh'C
Cuts at: 503 541 1215 1637 1762 2373 2466 2540 2730
Size: 38 674 422 125 611 93 74 190
Cuts at: 2730 2792 3522 503
Size: 62 730 1729
Fragments arranged by size:
1729 730 674 611 422 190 125 93
74 62 38
BspEI T'CCGG_A
Cuts at: 3968 3968
Size: 4748
BspHI T'CATG_A
Cuts at: 3924 3924
Size: 4748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 223 1798 2012 2314 2695 223
Size: 1575 214 302 381 2276
Fragments arranged by size:
2276 1575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1741 3040 3094 4578 4743 50
Size: 1691 1299 54 1484 165 55
Fragments arranged by size:
1691 1484 1299 165 55 54
BsrDI GCAATG_nn'
Cuts at: 163 1089 2660 163
Size: 926 1571 2251
Fragments arranged by size:
2251 1571 926
BsrFI r'CCGG_y
Cuts at: 2746 2927 3136 2746
Size: 181 209 4358
Fragments arranged by size:
4358 209 181
BssSI C'ACGA_G
Cuts at: 3019 3377 3019
Size: 358 4390
BstAPI GCAn_nnn'nTGC
Cuts at: 2125 2125
Size: 4748
BstBI TT'CG_AA
Cuts at: 1377 3109 1377
Size: 1732 3016
BstXI CCAn_nnnn'nTGG
Cuts at: 158 158
Size: 4748
BstYI r'GATC_y
Cuts at: 1 574 1773 2240 2263 2598 2844 3856 3942
Size: 573 1199 467 23 335 246 1012 86
Cuts at: 3942 3954 4300 1
Size: 12 346 449
Fragments arranged by size:
1199 1012 573 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4748
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1691 2698 2879 3042 1691
Size: 1007 181 163 3397
Fragments arranged by size:
3397 1007 181 163
BtsI GCAGTG_nn'
Cuts at: 1264 1684 1991 1264
Size: 420 307 4021
Fragments arranged by size:
4021 420 307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1242 1277 1662 1697 1242
Size: 35 385 35 4293
Fragments arranged by size:
4293 385 35 35
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4748
DraIII CAC_nnn'GTG
Cuts at: 545 1567 545
Size: 1022 3726
DrdI GACnn_nn'nnGTC
Cuts at: 2454 3312 2454
Size: 858 3890
EaeI y'GGCC_r
Cuts at: 2333 2507 2898 2925 4270 2333
Size: 174 391 27 1345 2811
Fragments arranged by size:
2811 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2333 2333
Size: 4748
EarI CTCTTCn'nnn_
Cuts at: 149 1127 2771 2981 149
Size: 978 1644 210 1916
Fragments arranged by size:
1916 1644 978 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2956 3266 3412 4558 2956
Size: 310 146 1146 3146
Fragments arranged by size:
3146 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1760 1760
Size: 4748
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 404 921 1001 1085 1173 1421 1425 1509
Size: 175 517 80 84 88 248 4 84
Cuts at: 1509 1673 1677 1757 2573 3005 3752 4020 229
Size: 164 4 80 816 432 747 268 957
Fragments arranged by size:
957 816 747 517 432 268 248 175
164 88 84 84 80 80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1887 1887
Size: 4748
EcoRI G'AATT_C
Cuts at: 1752 1752
Size: 4748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2767 2767
Size: 4748
FspI TGC'GCA
Cuts at: 2529 2529
Size: 4748
HaeII r_GCGC'y
Cuts at: 2182 2430 3452 4431 2182
Size: 248 1022 979 2499
Fragments arranged by size:
2499 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 950 982 2590 2620 2622 2652 331
Size: 32 587 32 1608 30 2 30 2427
Fragments arranged by size:
2427 1608 587 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1787 1787
Size: 4748
HindIII A'AGCT_T
Cuts at: 764 764
Size: 4748
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4500
KpnI G_GTAC'C
Cuts at: 1197 1768 1197
Size: 571 4177
MluI A'CGCG_T
Cuts at: 4181 4181
Size: 4748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3419 3603 3419
Size: 184 4564
MscI TGG'CCA
Cuts at: 2509 2509
Size: 4748
MslI CAynn'nnrTG
Cuts at: 1222 2737 2864 1222
Size: 1515 127 3106
Fragments arranged by size:
3106 1515 127
MspA1I CmG'CkG
Cuts at: 426 623 941 1025 1316 1445 1484 1859 2173
Size: 197 318 84 291 129 39 375 314
Cuts at: 2173 2533 3546 3791 426
Size: 360 1013 245 1383
Fragments arranged by size:
1383 1013 375 360 318 314 291 245
197 129 84 39
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 4748
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4500
NcoI C'CATG_G
Cuts at: 107 107
Size: 4748
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 4748
NsiI A_TGCA'T
Cuts at: 831 831
Size: 4748
NspI r_CATG'y
Cuts at: 799 833 967 1303 1471 1555 1723 1735 1801
Size: 34 134 336 168 84 168 12 66
Cuts at: 1801 2301 3208 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500 336 168 168 134 84
66 34 12
PciI A'CATG_T
Cuts at: 795 963 1299 1719 3204 795
Size: 168 336 420 1485 2339
Fragments arranged by size:
2339 1485 420 336 168
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4748
PmlI CAC'GTG
Cuts at: 1050 1386 1638 1050
Size: 336 252 4160
Fragments arranged by size:
4160 336 252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 3887 3919 214
Size: 32 3641 32 1043
Fragments arranged by size:
3641 1043 32 32
PstI C_TGCA'G
Cuts at: 220 622 1795 2480 220
Size: 402 1173 685 2488
Fragments arranged by size:
2488 1173 685 402
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4748
PvuII CAG'CTG
Cuts at: 426 623 941 1025 1445 2173 2533 426
Size: 197 318 84 420 728 360 2641
Fragments arranged by size:
2641 728 420 360 318 197 84
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4748
SacI G_AGCT'C
Cuts at: 1762 1762
Size: 4748
SalI G'TCGA_C
Cuts at: 1785 1785
Size: 4748
SapI GCTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4538
SbfI CC_TGCA'GG
Cuts at: 220 1795 220
Size: 1575 3173
ScaI AGT'ACT
Cuts at: 1540 4265 1540
Size: 2725 2023
SfcI C'TryA_G
Cuts at: 33 192 216 618 662 1356 1608 1791 2476
Size: 159 24 402 44 694 252 183 685
Cuts at: 2476 3469 3660 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 694 685 402 252 191 183
159 44 24
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4500
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
SmaI CCC'GGG
Cuts at: 1770 1770
Size: 4748
SmlI C'TyrA_G
Cuts at: 474 690 1903 2818 3310 3572 474
Size: 216 1213 915 492 262 1650
Fragments arranged by size:
1650 1213 915 492 262 216
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4748
SphI G_CATG'C
Cuts at: 1801 2301 1801
Size: 500 4248
StuI AGG'CCT
Cuts at: 1270 1270
Size: 4748
StyI C'CwwG_G
Cuts at: 107 233 1882 2236 107
Size: 126 1649 354 2619
Fragments arranged by size:
2619 1649 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2331 2464 2923 3132 2331
Size: 133 459 209 3947
Fragments arranged by size:
3947 459 209 133
TatI w'GTAC_w
Cuts at: 1538 1588 4263 1538
Size: 50 2675 2023
Fragments arranged by size:
2675 2023 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 423 455 4265 4297 423
Size: 32 3810 32 874
Fragments arranged by size:
3810 874 32 32
Tth111I GACn'n_nGTC
Cuts at: 2545 2545
Size: 4748
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2243 2243
Size: 4748
XmaI C'CCGG_G
Cuts at: 1768 1768
Size: 4748
XmnI GAAnn'nnTTC
Cuts at: 339 934 1270 1438 1522 1690 339
Size: 595 336 168 84 168 3397
Fragments arranged by size:
3397 595 336 168 168 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsgI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI