Restriction map for pF1KB7842
 (Circular) (Six-base) MAPSORT of: pF1KB7842.seq  Check: 9236  from: 1  to: 4964



pF1KB7842  4964 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:   2002    2002 
   Size:       4964
 
Acc65I G'GTAC_C

Cuts at:   1980    1980 
   Size:       4964
 
AclI AA'CG_TT

Cuts at:   4804    4804 
   Size:       4964
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    179     279     982    1299    2789    3221    3968    4236     179 
   Size:        100     703     317    1490     432     747     268     907

  Fragments arranged by size: 

                1490     907     747     703     432     317     268     100
            
 
AflIII A'CryG_T

Cuts at:    703    1132    2316    3420    4397     703 
   Size:        429    1184    1104     977    1270

  Fragments arranged by size: 

                1270    1184    1104     977     429
 
AgeI A'CCGG_T

Cuts at:   3352    3352 
   Size:       4964
 
AleI CACnn'nnGTG

Cuts at:   1526    1526 
   Size:       4964
 
AlwNI CAG_nnn'CTG

Cuts at:   2269    3836    2269 
   Size:       1567    3397
 
ApaLI G'TGCA_C

Cuts at:   3734    3734 
   Size:       4964
 
ApoI r'AATT_y

Cuts at:    151     402     808    1407    1744    1909    1968    4317    4573 
   Size:        251     406     599     337     165      59    2349     256

Cuts at:   4573     151 
   Size:        542

  Fragments arranged by size: 

                2349     599     542     406     337     256     251     165
                  59
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4964
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4964
 
AvaI C'yCGr_G

Cuts at:   1984    1984 
   Size:       4964
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1725    1758    1725 
   Size:         33    4931
 
BamHI G'GATC_C

Cuts at:   1989    1989 
   Size:       4964
 
BanI G'GyrC_C

Cuts at:   1980    2394    2642    2677    1980 
   Size:        414     248      35    4267

  Fragments arranged by size: 

                4267     414     248      35
 
BanII G_rGCy'C

Cuts at:    823    1978    3008     823 
   Size:       1155    1030    2779

  Fragments arranged by size: 

                2779    1155    1030
 
BbeI G_GCGC'C

Cuts at:   2398    2646    2398 
   Size:        248    4716
 
BbsI GAAGACnn'nnnn_

Cuts at:    213     385    1315     213 
   Size:        172     930    3862

  Fragments arranged by size: 

                3862     930     172
 
BciVI GTATCCnnnnn_n'

Cuts at:   2862    3623    4964    2862 
   Size:        761    1341    2862

  Fragments arranged by size: 

                2862    1341     761
 
BclI T'GATC_A

Cuts at:   2484    2484 
   Size:       4964
 
BglII A'GATC_T

Cuts at:   1898    2479    4516    1898 
   Size:        581    2037    2346

  Fragments arranged by size: 

                2346    2037     581
 
BlpI GC'TnA_GC

Cuts at:   2076    2076 
   Size:       4964
 
Bme1580I G_kGCm'C

Cuts at:   2589    2682    3738    2589 
   Size:         93    1056    3815

  Fragments arranged by size: 

                3815    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1031    2176    2257    2592    4736    1031 
   Size:       1145      81     335    2144    1259

  Fragments arranged by size: 

                2144    1259    1145     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1670    1920    3331    1670 
   Size:        250    1411    3303

  Fragments arranged by size: 

                3303    1411     250
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    875    1715    1904     875 
   Size:        840     189    3935

  Fragments arranged by size: 

                3935     840     189
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1522    2140    3019    3511    3809    1522 
   Size:        618     879     492     298    2677

  Fragments arranged by size: 

                2677     879     618     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    518    1527    4668     518 
   Size:       1009    3141     814

  Fragments arranged by size: 

                3141    1009     814
 
BsaAI yAC'GTr

Cuts at:    600    2317     600 
   Size:       1717    3247
 
BsaBI GATnn'nnATC

Cuts at:      6     668    2478    4515       6 
   Size:        662    1810    2037     455

  Fragments arranged by size: 

                2037    1810     662     455
 
BsaHI Gr'CG_yC

Cuts at:   2395    2643    3345    4841    2395 
   Size:        248     702    1496    2518

  Fragments arranged by size: 

                2518    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2153    2674    3352    3626    3773    4184    2153 
   Size:        521     678     274     147     411    2933

  Fragments arranged by size: 

                2933     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    244     247    1480     244 
   Size:          3    1233    3728

  Fragments arranged by size: 

                3728    1233       3
 
BseYI C'CCAG_C

Cuts at:    237     738    1571    1823    2389    3147    3724    4220     237 
   Size:        501     833     252     566     758     577     496     981

  Fragments arranged by size: 

                 981     833     758     577     566     501     496     252
            
 
BsiEI CG_ry'CG

Cuts at:    104    2506    2552    3760     104 
   Size:       2402      46    1208    1308

  Fragments arranged by size: 

                2402    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    823    1978    2756    2946    3738     823 
   Size:       1155     778     190     792    2049

  Fragments arranged by size: 

                2049    1155     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1089    1089 
   Size:       4964
 
Bsp1286I G_dGCh'C

Cuts at:    823    1978    2589    2682    2756    2946    3008    3738     823 
   Size:       1155     611      93      74     190      62     730    2049

  Fragments arranged by size: 

                2049    1155     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   4184    4184 
   Size:       4964
 
BspHI T'CATG_A

Cuts at:    984    4140     984 
   Size:       3156    1808
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    311    2014    2228    2530    2911     311 
   Size:       1703     214     302     381    2364

  Fragments arranged by size: 

                2364    1703     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3256    3310    4794    4959      50 
   Size:       3206      54    1484     165      55

  Fragments arranged by size: 

                3206    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    886     921    1222    2876     886 
   Size:         35     301    1654    2974

  Fragments arranged by size: 

                2974    1654     301      35
 
BsrFI r'CCGG_y

Cuts at:   2962    3143    3352    2962 
   Size:        181     209    4574

  Fragments arranged by size: 

                4574     209     181
 
BssSI C'ACGA_G

Cuts at:   3235    3593    3235 
   Size:        358    4606
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2341    2341 
   Size:       4964
 
BstBI TT'CG_AA

Cuts at:   3325    3325 
   Size:       4964
 
BstYI r'GATC_y

Cuts at:      1     416    1432    1497    1898    1989    2456    2479    2814 
   Size:        415    1016      65     401      91     467      23     335

Cuts at:   2814    3060    4072    4158    4170    4516       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1016    1012     467     449     415     401     346     335
                 246      91      86      65      23      12
 
BtgI C'CryG_G

Cuts at:   1094    1178    1094 
   Size:         84    4880
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2914    3095    3258    2914 
   Size:        181     163    4620

  Fragments arranged by size: 

                4620     181     163
 
BtsI GCAGTG_nn'

Cuts at:    200    1726    2207     200 
   Size:       1526     481    2957

  Fragments arranged by size: 

                2957    1526     481
 
DraI TTT'AAA

Cuts at:   1963    1963 
   Size:       4964
 
DrdI GACnn_nn'nnGTC

Cuts at:   2670    3528    2670 
   Size:        858    4106
 
EaeI y'GGCC_r

Cuts at:   2549    2723    3114    3141    4486    2549 
   Size:        174     391      27    1345    3027

  Fragments arranged by size: 

                3027    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2549    2549 
   Size:       4964
 
EarI CTCTTCn'nnn_

Cuts at:    455    1187    1628    1889    2987    3197     455 
   Size:        732     441     261    1098     210    2222

  Fragments arranged by size: 

                2222    1098     732     441     261     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3172    3482    3628    4774    3172 
   Size:        310     146    1146    3362

  Fragments arranged by size: 

                3362    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    821    1976     821 
   Size:       1155    3809
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    179     279     875     982    1299    1715    1904    2789    3221 
   Size:        100     596     107     317     416     189     885     432

Cuts at:   3221    3968    4236     179 
   Size:        747     268     907

  Fragments arranged by size: 

                 907     885     747     596     432     416     317     268
                 189     107     100
 
EcoO109I rG'GnC_Cy

Cuts at:   2103    2103 
   Size:       4964
 
EcoRI G'AATT_C

Cuts at:    402    1909    1968     402 
   Size:       1507      59    3398

  Fragments arranged by size: 

                3398    1507      59
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2983    2983 
   Size:       4964
 
FspI TGC'GCA

Cuts at:   2745    2745 
   Size:       4964
 
HaeII r_GCGC'y

Cuts at:   2398    2646    3668    4647    2398 
   Size:        248    1022     979    2715

  Fragments arranged by size: 

                2715    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    401     433     810     842    1482    1514    2806    2836    2838 
   Size:         32     377      32     640      32    1292      30       2

Cuts at:   2838    2868     401 
   Size:         30    2497

  Fragments arranged by size: 

                2497    1292     640     377      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2003    2003 
   Size:       4964
 
HindIII A'AGCT_T

Cuts at:    723     723 
   Size:       4964
 
KasI G'GCGC_C

Cuts at:   2394    2642    2394 
   Size:        248    4716
 
KpnI G_GTAC'C

Cuts at:   1984    1984 
   Size:       4964
 
MluI A'CGCG_T

Cuts at:   4397    4397 
   Size:       4964
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1475    3635    3819    1475 
   Size:       2160     184    2620

  Fragments arranged by size: 

                2620    2160     184
 
MscI TGG'CCA

Cuts at:   2725    2725 
   Size:       4964
 
MslI CAynn'nnrTG

Cuts at:    704    1038    1122    1206    1526    2953    3080     704 
   Size:        334      84      84     320    1427     127    2588

  Fragments arranged by size: 

                2588    1427     334     320     127      84      84
 
MspA1I CmG'CkG

Cuts at:   2075    2389    2749    3762    4007    2075 
   Size:        314     360    1013     245    3032

  Fragments arranged by size: 

                3032    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   3145    3145 
   Size:       4964
 
NarI GG'CG_CC

Cuts at:   2395    2643    2395 
   Size:        248    4716
 
NcoI C'CATG_G

Cuts at:   1094    1178    1094 
   Size:         84    4880
 
NdeI CA'TA_TG

Cuts at:    767     767 
   Size:       4964
 
NgoMIV G'CCGG_C

Cuts at:   3143    3143 
   Size:       4964
 
NspI r_CATG'y

Cuts at:    707    1136    1688    1761    2017    2517    3424     707 
   Size:        429     552      73     256     500     907    2247

  Fragments arranged by size: 

                2247     907     552     500     429     256      73
 
PciI A'CATG_T

Cuts at:    703    1132    3420     703 
   Size:        429    2288    2247

  Fragments arranged by size: 

                2288    2247     429
 
PmeI GTTT'AAAC

Cuts at:   1963    1963 
   Size:       4964
 
PmlI CAC'GTG

Cuts at:    600     600 
   Size:       4964
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    292     324    4103    4135     292 
   Size:         32    3779      32    1121

  Fragments arranged by size: 

                3779    1121      32      32
 
PsiI TTA'TAA

Cuts at:    953     953 
   Size:       4964
 
PstI C_TGCA'G

Cuts at:   2011    2696    2011 
   Size:        685    4279
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4964
 
PvuII CAG'CTG

Cuts at:   2389    2749    2389 
   Size:        360    4604
 
RsrII CG'GwC_CG

Cuts at:   3159    3159 
   Size:       4964
 
SacI G_AGCT'C

Cuts at:    823    1978     823 
   Size:       1155    3809
 
SalI G'TCGA_C

Cuts at:   2001    2001 
   Size:       4964
 
SapI GCTCTTCn'nnn_

Cuts at:   2987    3197    2987 
   Size:        210    4754
 
SbfI CC_TGCA'GG

Cuts at:   2011    2011 
   Size:       4964
 
ScaI AGT'ACT

Cuts at:   4481    4481 
   Size:       4964
 
SexAI A'CCwGG_T

Cuts at:    508     508 
   Size:       4964
 
SfcI C'TryA_G

Cuts at:     33    2007    2692    3685    3876      33 
   Size:       1974     685     993     191    1121

  Fragments arranged by size: 

                1974    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   2396    2644    2396 
   Size:        248    4716
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4964
 
SmaI CCC'GGG

Cuts at:   1986    1986 
   Size:       4964
 
SmlI C'TyrA_G

Cuts at:   1501    2119    3034    3526    3788    1501 
   Size:        618     915     492     262    2677

  Fragments arranged by size: 

                2677     915     618     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4964
 
SphI G_CATG'C

Cuts at:   2017    2517    2017 
   Size:        500    4464
 
StyI C'CwwG_G

Cuts at:   1094    1178    2098    2452    1094 
   Size:         84     920     354    3606

  Fragments arranged by size: 

                3606     920     354      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2547    2680    3139    3348    2547 
   Size:        133     459     209    4163

  Fragments arranged by size: 

                4163     459     209     133
 
TatI w'GTAC_w

Cuts at:   1660    4479    1660 
   Size:       2819    2145
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4481    4513    4481 
   Size:         32    4932
 
Tth111I GACn'n_nGTC

Cuts at:   2761    2761 
   Size:       4964
 
XbaI T'CTAG_A

Cuts at:   1995    1995 
   Size:       4964
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    461    2459     461 
   Size:       1998    2966
 
XmaI C'CCGG_G

Cuts at:   1984    1984 
   Size:       4964
 
XmnI GAAnn'nnTTC

Cuts at:    131     865     892     976    1033    1312     131 
   Size:        734      27      84      57     279    3783

  Fragments arranged by size: 

                3783     734     279      84      57      27
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PciI     PmeI     PmlI     PpiI     PsiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI     HpaI     MfeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI    PspXI
     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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