(Circular) (Six-base) MAPSORT of: pF1KB7842.seq Check: 9236 from: 1 to: 4964 pF1KB7842 4964 bp With 182 enzymes: SgfI * October 7, 2009 14:24 .. AccI GT'mk_AC Cuts at: 2002 2002 Size: 4964 Acc65I G'GTAC_C Cuts at: 1980 1980 Size: 4964 AclI AA'CG_TT Cuts at: 4804 4804 Size: 4964 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 179 279 982 1299 2789 3221 3968 4236 179 Size: 100 703 317 1490 432 747 268 907 Fragments arranged by size: 1490 907 747 703 432 317 268 100 AflIII A'CryG_T Cuts at: 703 1132 2316 3420 4397 703 Size: 429 1184 1104 977 1270 Fragments arranged by size: 1270 1184 1104 977 429 AgeI A'CCGG_T Cuts at: 3352 3352 Size: 4964 AleI CACnn'nnGTG Cuts at: 1526 1526 Size: 4964 AlwNI CAG_nnn'CTG Cuts at: 2269 3836 2269 Size: 1567 3397 ApaLI G'TGCA_C Cuts at: 3734 3734 Size: 4964 ApoI r'AATT_y Cuts at: 151 402 808 1407 1744 1909 1968 4317 4573 Size: 251 406 599 337 165 59 2349 256 Cuts at: 4573 151 Size: 542 Fragments arranged by size: 2349 599 542 406 337 256 251 165 59 AseI AT'TA_AT Cuts at: 20 20 Size: 4964 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4964 AvaI C'yCGr_G Cuts at: 1984 1984 Size: 4964 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1725 1758 1725 Size: 33 4931 BamHI G'GATC_C Cuts at: 1989 1989 Size: 4964 BanI G'GyrC_C Cuts at: 1980 2394 2642 2677 1980 Size: 414 248 35 4267 Fragments arranged by size: 4267 414 248 35 BanII G_rGCy'C Cuts at: 823 1978 3008 823 Size: 1155 1030 2779 Fragments arranged by size: 2779 1155 1030 BbeI G_GCGC'C Cuts at: 2398 2646 2398 Size: 248 4716 BbsI GAAGACnn'nnnn_ Cuts at: 213 385 1315 213 Size: 172 930 3862 Fragments arranged by size: 3862 930 172 BciVI GTATCCnnnnn_n' Cuts at: 2862 3623 4964 2862 Size: 761 1341 2862 Fragments arranged by size: 2862 1341 761 BclI T'GATC_A Cuts at: 2484 2484 Size: 4964 BglII A'GATC_T Cuts at: 1898 2479 4516 1898 Size: 581 2037 2346 Fragments arranged by size: 2346 2037 581 BlpI GC'TnA_GC Cuts at: 2076 2076 Size: 4964 Bme1580I G_kGCm'C Cuts at: 2589 2682 3738 2589 Size: 93 1056 3815 Fragments arranged by size: 3815 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1031 2176 2257 2592 4736 1031 Size: 1145 81 335 2144 1259 Fragments arranged by size: 2144 1259 1145 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1670 1920 3331 1670 Size: 250 1411 3303 Fragments arranged by size: 3303 1411 250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 875 1715 1904 875 Size: 840 189 3935 Fragments arranged by size: 3935 840 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1522 2140 3019 3511 3809 1522 Size: 618 879 492 298 2677 Fragments arranged by size: 2677 879 618 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 518 1527 4668 518 Size: 1009 3141 814 Fragments arranged by size: 3141 1009 814 BsaAI yAC'GTr Cuts at: 600 2317 600 Size: 1717 3247 BsaBI GATnn'nnATC Cuts at: 6 668 2478 4515 6 Size: 662 1810 2037 455 Fragments arranged by size: 2037 1810 662 455 BsaHI Gr'CG_yC Cuts at: 2395 2643 3345 4841 2395 Size: 248 702 1496 2518 Fragments arranged by size: 2518 1496 702 248 BsaWI w'CCGG_w Cuts at: 2153 2674 3352 3626 3773 4184 2153 Size: 521 678 274 147 411 2933 Fragments arranged by size: 2933 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 244 247 1480 244 Size: 3 1233 3728 Fragments arranged by size: 3728 1233 3 BseYI C'CCAG_C Cuts at: 237 738 1571 1823 2389 3147 3724 4220 237 Size: 501 833 252 566 758 577 496 981 Fragments arranged by size: 981 833 758 577 566 501 496 252 BsiEI CG_ry'CG Cuts at: 104 2506 2552 3760 104 Size: 2402 46 1208 1308 Fragments arranged by size: 2402 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 823 1978 2756 2946 3738 823 Size: 1155 778 190 792 2049 Fragments arranged by size: 2049 1155 792 778 190 BsmI GAATG_Cn' Cuts at: 1089 1089 Size: 4964 Bsp1286I G_dGCh'C Cuts at: 823 1978 2589 2682 2756 2946 3008 3738 823 Size: 1155 611 93 74 190 62 730 2049 Fragments arranged by size: 2049 1155 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 4184 4184 Size: 4964 BspHI T'CATG_A Cuts at: 984 4140 984 Size: 3156 1808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 311 2014 2228 2530 2911 311 Size: 1703 214 302 381 2364 Fragments arranged by size: 2364 1703 381 302 214 BsrBI CCG'CTC Cuts at: 50 3256 3310 4794 4959 50 Size: 3206 54 1484 165 55 Fragments arranged by size: 3206 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 886 921 1222 2876 886 Size: 35 301 1654 2974 Fragments arranged by size: 2974 1654 301 35 BsrFI r'CCGG_y Cuts at: 2962 3143 3352 2962 Size: 181 209 4574 Fragments arranged by size: 4574 209 181 BssSI C'ACGA_G Cuts at: 3235 3593 3235 Size: 358 4606 BstAPI GCAn_nnn'nTGC Cuts at: 2341 2341 Size: 4964 BstBI TT'CG_AA Cuts at: 3325 3325 Size: 4964 BstYI r'GATC_y Cuts at: 1 416 1432 1497 1898 1989 2456 2479 2814 Size: 415 1016 65 401 91 467 23 335 Cuts at: 2814 3060 4072 4158 4170 4516 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1016 1012 467 449 415 401 346 335 246 91 86 65 23 12 BtgI C'CryG_G Cuts at: 1094 1178 1094 Size: 84 4880 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2914 3095 3258 2914 Size: 181 163 4620 Fragments arranged by size: 4620 181 163 BtsI GCAGTG_nn' Cuts at: 200 1726 2207 200 Size: 1526 481 2957 Fragments arranged by size: 2957 1526 481 DraI TTT'AAA Cuts at: 1963 1963 Size: 4964 DrdI GACnn_nn'nnGTC Cuts at: 2670 3528 2670 Size: 858 4106 EaeI y'GGCC_r Cuts at: 2549 2723 3114 3141 4486 2549 Size: 174 391 27 1345 3027 Fragments arranged by size: 3027 1345 391 174 27 EagI C'GGCC_G Cuts at: 2549 2549 Size: 4964 EarI CTCTTCn'nnn_ Cuts at: 455 1187 1628 1889 2987 3197 455 Size: 732 441 261 1098 210 2222 Fragments arranged by size: 2222 1098 732 441 261 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3172 3482 3628 4774 3172 Size: 310 146 1146 3362 Fragments arranged by size: 3362 1146 310 146 EcoICRI GAG'CTC Cuts at: 821 1976 821 Size: 1155 3809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 179 279 875 982 1299 1715 1904 2789 3221 Size: 100 596 107 317 416 189 885 432 Cuts at: 3221 3968 4236 179 Size: 747 268 907 Fragments arranged by size: 907 885 747 596 432 416 317 268 189 107 100 EcoO109I rG'GnC_Cy Cuts at: 2103 2103 Size: 4964 EcoRI G'AATT_C Cuts at: 402 1909 1968 402 Size: 1507 59 3398 Fragments arranged by size: 3398 1507 59 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2983 2983 Size: 4964 FspI TGC'GCA Cuts at: 2745 2745 Size: 4964 HaeII r_GCGC'y Cuts at: 2398 2646 3668 4647 2398 Size: 248 1022 979 2715 Fragments arranged by size: 2715 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 401 433 810 842 1482 1514 2806 2836 2838 Size: 32 377 32 640 32 1292 30 2 Cuts at: 2838 2868 401 Size: 30 2497 Fragments arranged by size: 2497 1292 640 377 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2003 2003 Size: 4964 HindIII A'AGCT_T Cuts at: 723 723 Size: 4964 KasI G'GCGC_C Cuts at: 2394 2642 2394 Size: 248 4716 KpnI G_GTAC'C Cuts at: 1984 1984 Size: 4964 MluI A'CGCG_T Cuts at: 4397 4397 Size: 4964 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1475 3635 3819 1475 Size: 2160 184 2620 Fragments arranged by size: 2620 2160 184 MscI TGG'CCA Cuts at: 2725 2725 Size: 4964 MslI CAynn'nnrTG Cuts at: 704 1038 1122 1206 1526 2953 3080 704 Size: 334 84 84 320 1427 127 2588 Fragments arranged by size: 2588 1427 334 320 127 84 84 MspA1I CmG'CkG Cuts at: 2075 2389 2749 3762 4007 2075 Size: 314 360 1013 245 3032 Fragments arranged by size: 3032 1013 360 314 245 NaeI GCC'GGC Cuts at: 3145 3145 Size: 4964 NarI GG'CG_CC Cuts at: 2395 2643 2395 Size: 248 4716 NcoI C'CATG_G Cuts at: 1094 1178 1094 Size: 84 4880 NdeI CA'TA_TG Cuts at: 767 767 Size: 4964 NgoMIV G'CCGG_C Cuts at: 3143 3143 Size: 4964 NspI r_CATG'y Cuts at: 707 1136 1688 1761 2017 2517 3424 707 Size: 429 552 73 256 500 907 2247 Fragments arranged by size: 2247 907 552 500 429 256 73 PciI A'CATG_T Cuts at: 703 1132 3420 703 Size: 429 2288 2247 Fragments arranged by size: 2288 2247 429 PmeI GTTT'AAAC Cuts at: 1963 1963 Size: 4964 PmlI CAC'GTG Cuts at: 600 600 Size: 4964 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 292 324 4103 4135 292 Size: 32 3779 32 1121 Fragments arranged by size: 3779 1121 32 32 PsiI TTA'TAA Cuts at: 953 953 Size: 4964 PstI C_TGCA'G Cuts at: 2011 2696 2011 Size: 685 4279 PvuI CG_AT'CG Cuts at: 104 104 Size: 4964 PvuII CAG'CTG Cuts at: 2389 2749 2389 Size: 360 4604 RsrII CG'GwC_CG Cuts at: 3159 3159 Size: 4964 SacI G_AGCT'C Cuts at: 823 1978 823 Size: 1155 3809 SalI G'TCGA_C Cuts at: 2001 2001 Size: 4964 SapI GCTCTTCn'nnn_ Cuts at: 2987 3197 2987 Size: 210 4754 SbfI CC_TGCA'GG Cuts at: 2011 2011 Size: 4964 ScaI AGT'ACT Cuts at: 4481 4481 Size: 4964 SexAI A'CCwGG_T Cuts at: 508 508 Size: 4964 SfcI C'TryA_G Cuts at: 33 2007 2692 3685 3876 33 Size: 1974 685 993 191 1121 Fragments arranged by size: 1974 1121 993 685 191 SfoI GGC'GCC Cuts at: 2396 2644 2396 Size: 248 4716 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4964 SmaI CCC'GGG Cuts at: 1986 1986 Size: 4964 SmlI C'TyrA_G Cuts at: 1501 2119 3034 3526 3788 1501 Size: 618 915 492 262 2677 Fragments arranged by size: 2677 915 618 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4964 SphI G_CATG'C Cuts at: 2017 2517 2017 Size: 500 4464 StyI C'CwwG_G Cuts at: 1094 1178 2098 2452 1094 Size: 84 920 354 3606 Fragments arranged by size: 3606 920 354 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2547 2680 3139 3348 2547 Size: 133 459 209 4163 Fragments arranged by size: 4163 459 209 133 TatI w'GTAC_w Cuts at: 1660 4479 1660 Size: 2819 2145 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4481 4513 4481 Size: 32 4932 Tth111I GACn'n_nGTC Cuts at: 2761 2761 Size: 4964 XbaI T'CTAG_A Cuts at: 1995 1995 Size: 4964 XcmI CCAnnnn_n'nnnnTGG Cuts at: 461 2459 461 Size: 1998 2966 XmaI C'CCGG_G Cuts at: 1984 1984 Size: 4964 XmnI GAAnn'nnTTC Cuts at: 131 865 892 976 1033 1312 131 Size: 734 27 84 57 279 3783 Fragments arranged by size: 3783 734 279 84 57 27 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PmlI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI