Restriction map for
pF1KB7842
(Circular) (Six-base) MAPSORT of: pF1KB7842.seq Check: 9236 from: 1 to: 4964
pF1KB7842 4964 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 2002 2002
Size: 4964
Acc65I G'GTAC_C
Cuts at: 1980 1980
Size: 4964
AclI AA'CG_TT
Cuts at: 4804 4804
Size: 4964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 279 982 1299 2789 3221 3968 4236 179
Size: 100 703 317 1490 432 747 268 907
Fragments arranged by size:
1490 907 747 703 432 317 268 100
AflIII A'CryG_T
Cuts at: 703 1132 2316 3420 4397 703
Size: 429 1184 1104 977 1270
Fragments arranged by size:
1270 1184 1104 977 429
AgeI A'CCGG_T
Cuts at: 3352 3352
Size: 4964
AleI CACnn'nnGTG
Cuts at: 1526 1526
Size: 4964
AlwNI CAG_nnn'CTG
Cuts at: 2269 3836 2269
Size: 1567 3397
ApaLI G'TGCA_C
Cuts at: 3734 3734
Size: 4964
ApoI r'AATT_y
Cuts at: 151 402 808 1407 1744 1909 1968 4317 4573
Size: 251 406 599 337 165 59 2349 256
Cuts at: 4573 151
Size: 542
Fragments arranged by size:
2349 599 542 406 337 256 251 165
59
AseI AT'TA_AT
Cuts at: 20 20
Size: 4964
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
AvaI C'yCGr_G
Cuts at: 1984 1984
Size: 4964
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1725 1758 1725
Size: 33 4931
BamHI G'GATC_C
Cuts at: 1989 1989
Size: 4964
BanI G'GyrC_C
Cuts at: 1980 2394 2642 2677 1980
Size: 414 248 35 4267
Fragments arranged by size:
4267 414 248 35
BanII G_rGCy'C
Cuts at: 823 1978 3008 823
Size: 1155 1030 2779
Fragments arranged by size:
2779 1155 1030
BbeI G_GCGC'C
Cuts at: 2398 2646 2398
Size: 248 4716
BbsI GAAGACnn'nnnn_
Cuts at: 213 385 1315 213
Size: 172 930 3862
Fragments arranged by size:
3862 930 172
BciVI GTATCCnnnnn_n'
Cuts at: 2862 3623 4964 2862
Size: 761 1341 2862
Fragments arranged by size:
2862 1341 761
BclI T'GATC_A
Cuts at: 2484 2484
Size: 4964
BglII A'GATC_T
Cuts at: 1898 2479 4516 1898
Size: 581 2037 2346
Fragments arranged by size:
2346 2037 581
BlpI GC'TnA_GC
Cuts at: 2076 2076
Size: 4964
Bme1580I G_kGCm'C
Cuts at: 2589 2682 3738 2589
Size: 93 1056 3815
Fragments arranged by size:
3815 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1031 2176 2257 2592 4736 1031
Size: 1145 81 335 2144 1259
Fragments arranged by size:
2144 1259 1145 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1670 1920 3331 1670
Size: 250 1411 3303
Fragments arranged by size:
3303 1411 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1715 1904 875
Size: 840 189 3935
Fragments arranged by size:
3935 840 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2140 3019 3511 3809 1522
Size: 618 879 492 298 2677
Fragments arranged by size:
2677 879 618 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 518 1527 4668 518
Size: 1009 3141 814
Fragments arranged by size:
3141 1009 814
BsaAI yAC'GTr
Cuts at: 600 2317 600
Size: 1717 3247
BsaBI GATnn'nnATC
Cuts at: 6 668 2478 4515 6
Size: 662 1810 2037 455
Fragments arranged by size:
2037 1810 662 455
BsaHI Gr'CG_yC
Cuts at: 2395 2643 3345 4841 2395
Size: 248 702 1496 2518
Fragments arranged by size:
2518 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2153 2674 3352 3626 3773 4184 2153
Size: 521 678 274 147 411 2933
Fragments arranged by size:
2933 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 247 1480 244
Size: 3 1233 3728
Fragments arranged by size:
3728 1233 3
BseYI C'CCAG_C
Cuts at: 237 738 1571 1823 2389 3147 3724 4220 237
Size: 501 833 252 566 758 577 496 981
Fragments arranged by size:
981 833 758 577 566 501 496 252
BsiEI CG_ry'CG
Cuts at: 104 2506 2552 3760 104
Size: 2402 46 1208 1308
Fragments arranged by size:
2402 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 823 1978 2756 2946 3738 823
Size: 1155 778 190 792 2049
Fragments arranged by size:
2049 1155 792 778 190
BsmI GAATG_Cn'
Cuts at: 1089 1089
Size: 4964
Bsp1286I G_dGCh'C
Cuts at: 823 1978 2589 2682 2756 2946 3008 3738 823
Size: 1155 611 93 74 190 62 730 2049
Fragments arranged by size:
2049 1155 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4184 4184
Size: 4964
BspHI T'CATG_A
Cuts at: 984 4140 984
Size: 3156 1808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 2014 2228 2530 2911 311
Size: 1703 214 302 381 2364
Fragments arranged by size:
2364 1703 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3256 3310 4794 4959 50
Size: 3206 54 1484 165 55
Fragments arranged by size:
3206 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 886 921 1222 2876 886
Size: 35 301 1654 2974
Fragments arranged by size:
2974 1654 301 35
BsrFI r'CCGG_y
Cuts at: 2962 3143 3352 2962
Size: 181 209 4574
Fragments arranged by size:
4574 209 181
BssSI C'ACGA_G
Cuts at: 3235 3593 3235
Size: 358 4606
BstAPI GCAn_nnn'nTGC
Cuts at: 2341 2341
Size: 4964
BstBI TT'CG_AA
Cuts at: 3325 3325
Size: 4964
BstYI r'GATC_y
Cuts at: 1 416 1432 1497 1898 1989 2456 2479 2814
Size: 415 1016 65 401 91 467 23 335
Cuts at: 2814 3060 4072 4158 4170 4516 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1016 1012 467 449 415 401 346 335
246 91 86 65 23 12
BtgI C'CryG_G
Cuts at: 1094 1178 1094
Size: 84 4880
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2914 3095 3258 2914
Size: 181 163 4620
Fragments arranged by size:
4620 181 163
BtsI GCAGTG_nn'
Cuts at: 200 1726 2207 200
Size: 1526 481 2957
Fragments arranged by size:
2957 1526 481
DraI TTT'AAA
Cuts at: 1963 1963
Size: 4964
DrdI GACnn_nn'nnGTC
Cuts at: 2670 3528 2670
Size: 858 4106
EaeI y'GGCC_r
Cuts at: 2549 2723 3114 3141 4486 2549
Size: 174 391 27 1345 3027
Fragments arranged by size:
3027 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2549 2549
Size: 4964
EarI CTCTTCn'nnn_
Cuts at: 455 1187 1628 1889 2987 3197 455
Size: 732 441 261 1098 210 2222
Fragments arranged by size:
2222 1098 732 441 261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3172 3482 3628 4774 3172
Size: 310 146 1146 3362
Fragments arranged by size:
3362 1146 310 146
EcoICRI GAG'CTC
Cuts at: 821 1976 821
Size: 1155 3809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 279 875 982 1299 1715 1904 2789 3221
Size: 100 596 107 317 416 189 885 432
Cuts at: 3221 3968 4236 179
Size: 747 268 907
Fragments arranged by size:
907 885 747 596 432 416 317 268
189 107 100
EcoO109I rG'GnC_Cy
Cuts at: 2103 2103
Size: 4964
EcoRI G'AATT_C
Cuts at: 402 1909 1968 402
Size: 1507 59 3398
Fragments arranged by size:
3398 1507 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4964
FspI TGC'GCA
Cuts at: 2745 2745
Size: 4964
HaeII r_GCGC'y
Cuts at: 2398 2646 3668 4647 2398
Size: 248 1022 979 2715
Fragments arranged by size:
2715 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 401 433 810 842 1482 1514 2806 2836 2838
Size: 32 377 32 640 32 1292 30 2
Cuts at: 2838 2868 401
Size: 30 2497
Fragments arranged by size:
2497 1292 640 377 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2003 2003
Size: 4964
HindIII A'AGCT_T
Cuts at: 723 723
Size: 4964
KasI G'GCGC_C
Cuts at: 2394 2642 2394
Size: 248 4716
KpnI G_GTAC'C
Cuts at: 1984 1984
Size: 4964
MluI A'CGCG_T
Cuts at: 4397 4397
Size: 4964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1475 3635 3819 1475
Size: 2160 184 2620
Fragments arranged by size:
2620 2160 184
MscI TGG'CCA
Cuts at: 2725 2725
Size: 4964
MslI CAynn'nnrTG
Cuts at: 704 1038 1122 1206 1526 2953 3080 704
Size: 334 84 84 320 1427 127 2588
Fragments arranged by size:
2588 1427 334 320 127 84 84
MspA1I CmG'CkG
Cuts at: 2075 2389 2749 3762 4007 2075
Size: 314 360 1013 245 3032
Fragments arranged by size:
3032 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3145 3145
Size: 4964
NarI GG'CG_CC
Cuts at: 2395 2643 2395
Size: 248 4716
NcoI C'CATG_G
Cuts at: 1094 1178 1094
Size: 84 4880
NdeI CA'TA_TG
Cuts at: 767 767
Size: 4964
NgoMIV G'CCGG_C
Cuts at: 3143 3143
Size: 4964
NspI r_CATG'y
Cuts at: 707 1136 1688 1761 2017 2517 3424 707
Size: 429 552 73 256 500 907 2247
Fragments arranged by size:
2247 907 552 500 429 256 73
PciI A'CATG_T
Cuts at: 703 1132 3420 703
Size: 429 2288 2247
Fragments arranged by size:
2288 2247 429
PmeI GTTT'AAAC
Cuts at: 1963 1963
Size: 4964
PmlI CAC'GTG
Cuts at: 600 600
Size: 4964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 292 324 4103 4135 292
Size: 32 3779 32 1121
Fragments arranged by size:
3779 1121 32 32
PsiI TTA'TAA
Cuts at: 953 953
Size: 4964
PstI C_TGCA'G
Cuts at: 2011 2696 2011
Size: 685 4279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4964
PvuII CAG'CTG
Cuts at: 2389 2749 2389
Size: 360 4604
RsrII CG'GwC_CG
Cuts at: 3159 3159
Size: 4964
SacI G_AGCT'C
Cuts at: 823 1978 823
Size: 1155 3809
SalI G'TCGA_C
Cuts at: 2001 2001
Size: 4964
SapI GCTCTTCn'nnn_
Cuts at: 2987 3197 2987
Size: 210 4754
SbfI CC_TGCA'GG
Cuts at: 2011 2011
Size: 4964
ScaI AGT'ACT
Cuts at: 4481 4481
Size: 4964
SexAI A'CCwGG_T
Cuts at: 508 508
Size: 4964
SfcI C'TryA_G
Cuts at: 33 2007 2692 3685 3876 33
Size: 1974 685 993 191 1121
Fragments arranged by size:
1974 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2396 2644 2396
Size: 248 4716
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
SmaI CCC'GGG
Cuts at: 1986 1986
Size: 4964
SmlI C'TyrA_G
Cuts at: 1501 2119 3034 3526 3788 1501
Size: 618 915 492 262 2677
Fragments arranged by size:
2677 915 618 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4964
SphI G_CATG'C
Cuts at: 2017 2517 2017
Size: 500 4464
StyI C'CwwG_G
Cuts at: 1094 1178 2098 2452 1094
Size: 84 920 354 3606
Fragments arranged by size:
3606 920 354 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2547 2680 3139 3348 2547
Size: 133 459 209 4163
Fragments arranged by size:
4163 459 209 133
TatI w'GTAC_w
Cuts at: 1660 4479 1660
Size: 2819 2145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4481 4513 4481
Size: 32 4932
Tth111I GACn'n_nGTC
Cuts at: 2761 2761
Size: 4964
XbaI T'CTAG_A
Cuts at: 1995 1995
Size: 4964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 461 2459 461
Size: 1998 2966
XmaI C'CCGG_G
Cuts at: 1984 1984
Size: 4964
XmnI GAAnn'nnTTC
Cuts at: 131 865 892 976 1033 1312 131
Size: 734 27 84 57 279 3783
Fragments arranged by size:
3783 734 279 84 57 27
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PmlI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI